FastQCFastQC Report
Tue 24 May 2016
ERR840940.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840940.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4642659
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC461550.9941501195758724No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA372470.8022773156503633No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC167840.3615169668933256No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT166270.3581352841119712No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC132460.2853106377186005No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT104100.22422495384649185No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA95460.2056149288586562No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90590.19512525042222573No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG81680.17593366215352021No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA78930.17001033244095679No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG64970.13994135688190754No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT64640.13923055731639994No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG58240.1254453536217069No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA58030.12499302662547476No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG54840.11812196415890118No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC53750.11577417165464876No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53170.11452488756981721No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC52890.11392178490817438No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG50870.10957082999203688No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG50380.10851540033416196No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG47120.10149356220217767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACT205.0269045E-7193.7799444
ACCGATA205.0269045E-7193.7799444
CGCAATT450.0193.7799444
TAAGCCA126800.0157.3315944
TAATCCG201.2187358E-4145.3349644
CTATCAT33000.0143.2797244
ACGGATC600.0129.1866344
GCCGTTA1600.0121.11246544
ACTTCTA8250.0112.744744
GCCGAAT452.1931737E-7107.65552544
TAATGCG401.6552804E-10103.6833743
TTAAGCC121550.0101.1426243
CGGTACG1000.096.8899744
CGTTCGA2350.093.2835143
ATGTCGC452.8371694E-586.1244244
CGTATCC359.189389E-784.6394843
CGACGGG36700.079.46561444
ATTAAGC167200.069.31589542
ATAAGAT6000.068.1347943
TAATTGC1050.067.71158643
Produced by FastQC (version 0.11.5)