##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840940.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4642659
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.0601743526716	39.0	37.0	39.0	33.0	39.0
2	37.64699238087484	39.0	37.0	39.0	35.0	39.0
3	37.78263576971731	39.0	37.0	39.0	35.0	39.0
4	38.128316983866355	39.0	38.0	40.0	35.0	41.0
5	38.86655233563352	40.0	38.0	41.0	35.0	41.0
6	38.84206529060179	40.0	38.0	41.0	35.0	41.0
7	38.766316027087065	40.0	38.0	41.0	35.0	41.0
8	38.63788768462211	40.0	38.0	41.0	35.0	41.0
9	38.547925229916736	40.0	38.0	41.0	35.0	41.0
10	38.36626575417234	40.0	38.0	41.0	34.0	41.0
11	38.31736683654776	40.0	37.0	41.0	34.0	41.0
12	38.30361544968088	40.0	37.0	41.0	34.0	41.0
13	38.17250437734066	40.0	37.0	41.0	34.0	41.0
14	38.18706607571222	40.0	37.0	41.0	34.0	41.0
15	38.12985769577305	40.0	37.0	41.0	34.0	41.0
16	38.07985488488386	40.0	37.0	41.0	34.0	41.0
17	38.09139590049581	40.0	36.0	41.0	34.0	41.0
18	38.189546335408224	40.0	37.0	41.0	34.0	41.0
19	38.1320913295592	40.0	36.0	41.0	34.0	41.0
20	38.21073699360647	40.0	36.0	41.0	34.0	41.0
21	38.119760680248106	40.0	36.0	41.0	34.0	41.0
22	37.989297512481535	40.0	35.0	41.0	33.0	41.0
23	37.83277470949299	39.0	35.0	41.0	33.0	41.0
24	37.70918798903818	39.0	35.0	41.0	33.0	41.0
25	37.60625584503208	39.0	35.0	41.0	33.0	41.0
26	37.49995166667067	39.0	35.0	41.0	33.0	41.0
27	37.375366912897704	39.0	35.0	41.0	33.0	41.0
28	37.29494649404002	39.0	35.0	41.0	33.0	41.0
29	37.137915403971	38.0	35.0	41.0	33.0	41.0
30	37.047081460359834	38.0	35.0	41.0	33.0	41.0
31	36.86885108974169	38.0	35.0	41.0	33.0	41.0
32	36.796151967661146	37.0	35.0	40.0	33.0	41.0
33	36.681688971818886	37.0	35.0	40.0	33.0	41.0
34	36.50974037580202	37.0	35.0	40.0	32.0	41.0
35	36.42513742557437	36.0	35.0	40.0	32.0	41.0
36	36.354010911342094	36.0	35.0	40.0	32.0	41.0
37	36.25865056926507	36.0	35.0	40.0	32.0	41.0
38	36.15418755692946	36.0	35.0	40.0	32.0	41.0
39	36.13546971646702	35.0	35.0	40.0	32.0	41.0
40	35.957875905561494	35.0	35.0	39.0	32.0	41.0
41	35.90309490351169	35.0	35.0	39.0	32.0	41.0
42	35.82225785280308	35.0	35.0	39.0	32.0	41.0
43	35.7371904166139	35.0	34.0	39.0	32.0	41.0
44	35.50571441280498	35.0	34.0	39.0	31.0	41.0
45	35.324475834473084	35.0	34.0	39.0	31.0	41.0
46	35.12327045538944	35.0	34.0	38.0	31.0	41.0
47	35.0070532753402	35.0	34.0	38.0	30.0	40.0
48	34.86451219928214	35.0	34.0	38.0	30.0	40.0
49	35.11787609629682	35.0	34.0	38.0	31.0	40.0
50	34.88975264066926	35.0	33.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	50.0
22	101.0
23	283.0
24	570.0
25	1296.0
26	2726.0
27	5906.0
28	12268.0
29	24040.0
30	44435.0
31	77727.0
32	128772.0
33	218433.0
34	392889.0
35	395898.0
36	566376.0
37	718513.0
38	733070.0
39	748600.0
40	570683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.686141713186345	21.413741564909248	35.90011672190441
2	27.03648921878605	24.047749360872725	28.105617922832582	20.810143497508648
3	26.35771871248782	17.602046585803524	26.617569802132785	29.422664899575867
4	19.251360050350456	20.98323396140014	28.656336810435572	31.109069177813836
5	23.419122533013947	20.412375752774434	32.611936392485426	23.556565321726193
6	26.675467657650497	23.862079898609828	23.45401632986614	26.008436113873536
7	32.02113271726397	18.70247201011317	21.054076984762396	28.22231828786047
8	26.67535996074663	19.692701962388366	27.00620484941927	26.625733227445735
9	24.341675750900507	24.874689267508124	27.468763051518536	23.31487193007283
10	30.920750371715865	18.53106161792197	21.83991975288299	28.708268257479176
11	26.17090766304396	24.721867360923987	23.468038466749334	25.63918650928272
12	25.60104026593381	20.680088716401528	25.948707410990124	27.770163606674537
13	24.261980042040562	20.365139890739336	23.356766025676233	32.016114041543865
14	23.41011907185085	21.97126689683649	20.948749412782632	33.66986461853003
15	23.69922064058549	23.50584008000588	26.921081216604538	25.873858062804096
16	28.06525312326406	23.527228685113425	19.031442972658557	29.37607521896396
17	27.1970222236869	23.285621450983154	18.713737106257426	30.80361921907252
18	26.426321640249697	22.570126300467038	17.977154901964585	33.026397157318684
19	25.686120819986996	27.48295750344792	21.050049120557855	25.780872556007235
20	32.42624539084176	20.193126395886495	20.11504614058452	27.265582072687227
21	28.30039854316244	23.806292902407865	22.52144729992015	25.371861254509536
22	26.974994286679248	26.859478587593877	20.906596844609954	25.25893028111692
23	32.769626199124254	22.153942385171945	20.155454018914593	24.92097739678921
24	29.603940328161084	22.79448049059817	20.269505040107404	27.332074141133344
25	26.34673446686795	27.511030123678054	20.9761214429425	25.166113966511496
26	28.13800594794533	23.025147078430265	24.816229699183395	24.02061727444101
27	25.735623635724224	29.51299575446588	19.372584426142527	25.37879618366737
28	25.614076073122057	21.920836965229668	24.451523963904002	28.013562997744273
29	33.59421409053107	23.581251695662118	20.66790468472654	22.156629529080273
30	27.59564814802431	26.090270078209045	19.083889151175764	27.23019262259088
31	25.329949192988504	23.891420108511635	26.281401867564075	24.497228830935786
32	24.780543917647435	22.238675686629048	25.236127922259527	27.744652473463987
33	29.13560208422355	22.068889802009693	22.655909050738032	26.13959906302872
34	24.655659945004583	25.223120989917508	25.702016498625113	24.419202566452796
35	23.05308095269421	22.486690233580198	22.477734344794793	31.9824944689308
36	23.812314891902904	22.500605618468718	29.73417293864644	23.952906550981936
37	24.283349156941036	24.786649492746676	22.672932675388424	28.257068674923865
38	26.04592452505318	27.590605662603206	20.210694422713278	26.152775389630328
39	24.646099990867402	29.186134402122725	19.676573845322093	26.491191761687784
40	24.18530050139191	29.659040673153143	20.572247635865995	25.58341118958895
41	29.927702594327766	23.663515425441506	20.071052549680527	26.3377294305502
42	25.66321138505639	27.520087443718964	21.321428054377254	25.495273116847393
43	24.552735539354025	21.72872548754053	26.85460863886898	26.863930334236464
44	26.336496175688467	22.59192872583666	25.63102569180912	25.440549406665752
45	25.944136816948532	26.254731212376836	25.976706072249506	21.824425898425122
46	24.760138867876087	29.698195038533658	21.166204767078824	24.375461326511434
47	32.13659340371684	21.543075335511062	21.28165156447833	25.038679696293777
48	24.041276536105048	22.7196448248353	20.611264941618618	32.627813697441034
49	26.050001493397595	21.965654397293328	19.84312334892186	32.14122076038722
50	22.64947579856508	33.59535643731366	20.578334249172382	23.176833514948875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57510.0
1	46921.5
2	34420.5
3	28311.5
4	20962.0
5	15388.5
6	9557.5
7	6719.0
8	7589.5
9	7691.5
10	7258.0
11	7169.5
12	7315.0
13	7609.5
14	8393.5
15	9353.5
16	9794.0
17	9265.0
18	8839.0
19	9999.5
20	11880.0
21	13554.0
22	16042.5
23	20125.0
24	23590.5
25	25965.0
26	30120.0
27	37256.0
28	44282.0
29	49886.5
30	55646.0
31	63032.0
32	68819.5
33	69319.5
34	73150.5
35	83374.0
36	93008.0
37	98474.0
38	102980.0
39	113766.5
40	147945.0
41	186622.0
42	183881.5
43	247867.0
44	327312.0
45	258701.5
46	187722.0
47	189227.5
48	208182.5
49	241176.0
50	276774.0
51	293440.0
52	294320.0
53	294952.5
54	301931.0
55	294299.5
56	282978.5
57	288220.0
58	280137.0
59	266029.5
60	264290.5
61	271362.5
62	274522.5
63	268296.5
64	260184.5
65	250755.0
66	229916.0
67	216057.0
68	203329.5
69	186271.5
70	180433.5
71	186600.5
72	182520.0
73	151433.0
74	120506.5
75	103235.5
76	85533.5
77	63414.0
78	52089.5
79	48941.5
80	40550.0
81	26875.5
82	16714.5
83	13552.0
84	11553.5
85	9084.5
86	6882.0
87	4783.5
88	2824.0
89	1796.5
90	1319.0
91	1084.5
92	777.5
93	530.0
94	356.0
95	112.5
96	28.0
97	28.0
98	135.0
99	242.0
100	242.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	36183.0
25	23717.0
26	21731.0
27	23683.0
28	25833.0
29	25330.0
30	32466.0
31	27090.0
32	36310.0
33	26316.0
34	31655.0
35	22701.0
36	21747.0
37	24946.0
38	25384.0
39	27886.0
40	32491.0
41	32810.0
42	46413.0
43	73844.0
44	189218.0
45	344969.0
46	586604.0
47	470279.0
48	859460.0
49	607266.0
50	966327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.372312633293719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72951014110278	9.369491750319108
2	9.809966129642959	2.427439357559301
3	3.3292373462919937	1.235710958362851
4	1.6709041344293782	0.8269179332569321
5	0.9593595965418318	0.5934748428083036
6	0.6937130112111753	0.5149700551492952
7	0.483625278916685	0.41884942036847705
8	0.3971418231369082	0.39308502364848547
9	0.3114202208074316	0.34676894989430146
>10	3.1744596536705743	9.242721572962562
>50	1.5609860657416097	14.221136734720444
>100	1.769803688987097	37.89040709873914
>500	0.06798765671931117	5.765319254317535
>1k	0.03581492630749428	8.067443914062187
>5k	0.004249228544956948	3.4113728503359115
>10k+	0.0018210979478386921	5.274890283495169
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	46155	0.9941501195758724	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	37247	0.8022773156503633	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	16784	0.3615169668933256	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	16627	0.3581352841119712	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	13246	0.2853106377186005	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	10410	0.22422495384649185	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	9546	0.2056149288586562	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9059	0.19512525042222573	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG	8168	0.17593366215352021	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	7893	0.17001033244095679	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG	6497	0.13994135688190754	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	6464	0.13923055731639994	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	5824	0.1254453536217069	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	5803	0.12499302662547476	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG	5484	0.11812196415890118	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	5375	0.11577417165464876	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5317	0.11452488756981721	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	5289	0.11392178490817438	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	5087	0.10957082999203688	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG	5038	0.10851540033416196	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	4712	0.10149356220217767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.011717423140489104	0.0	0.0	0.0	0.0
2	0.027656564912477956	0.0	0.0	0.0	0.0
3	0.0371554318333524	0.0	0.0	0.0	0.0
4	0.04751587398514515	0.0	0.0	0.0	0.0
5	0.06705209234621798	0.0	0.0	0.0	0.0
6	0.08184964693724006	0.0	0.0	0.0	0.0
7	0.09404093645473424	0.0	0.0	0.0	0.0
8	0.10422906356034332	0.0	0.0	0.0	0.0
9	0.1144818088082713	0.0	0.0	0.0	0.0
10	0.1235929668752325	0.0	0.0	0.0	0.0
11	0.13528885063494864	0.0	0.0	0.0	0.0
12	0.1445723237480935	0.0	0.0	0.0	0.0
13	0.15385579686123835	0.0	0.0	0.0	0.0
14	0.16447471158230662	0.0	0.0	0.0	0.0
15	0.18224470071999688	0.0	0.0	0.0	0.0
16	0.19198050082937385	0.0	0.0	0.0	0.0
17	0.20875967845150806	0.0	0.0	0.0	0.0
18	0.23085908312456288	0.0	0.0	0.0	0.0
19	0.25892489627172705	0.0	0.0	0.0	0.0
20	0.2701253742736652	0.0	0.0	0.0	0.0
21	0.2874861151766692	0.0	0.0	0.0	2.153938077295791E-5
22	0.2984496599901048	0.0	0.0	0.0	2.153938077295791E-5
23	0.31221332430402493	0.0	0.0	0.0	2.153938077295791E-5
24	0.32436153505997317	0.0	0.0	0.0	4.307876154591582E-5
25	0.33879292017785495	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
26	0.3716189364758428	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
27	0.38798886586329084	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
28	0.402743341692767	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
29	0.4169808723836922	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
30	0.42990450084746695	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
31	0.44304352311897127	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
32	0.45657025424438885	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
33	0.46740456277318665	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
34	0.4797466279560915	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
35	0.4912055785273052	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
36	0.5007044454481796	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
37	0.508157071195623	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
38	0.5132188256772682	0.0	0.0	2.153938077295791E-5	4.307876154591582E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACT	20	5.0269045E-7	193.77994	44
ACCGATA	20	5.0269045E-7	193.77994	44
CGCAATT	45	0.0	193.77994	44
TAAGCCA	12680	0.0	157.33159	44
TAATCCG	20	1.2187358E-4	145.33496	44
CTATCAT	3300	0.0	143.27972	44
ACGGATC	60	0.0	129.18663	44
GCCGTTA	160	0.0	121.112465	44
ACTTCTA	825	0.0	112.7447	44
GCCGAAT	45	2.1931737E-7	107.655525	44
TAATGCG	40	1.6552804E-10	103.68337	43
TTAAGCC	12155	0.0	101.14262	43
CGGTACG	100	0.0	96.88997	44
CGTTCGA	235	0.0	93.28351	43
ATGTCGC	45	2.8371694E-5	86.12442	44
CGTATCC	35	9.189389E-7	84.63948	43
CGACGGG	3670	0.0	79.465614	44
ATTAAGC	16720	0.0	69.315895	42
ATAAGAT	600	0.0	68.13479	43
TAATTGC	105	0.0	67.711586	43
>>END_MODULE