FastQCFastQC Report
Tue 24 May 2016
ERR840939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7118176
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA379920.5337322370225182No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159460.22401806305435548No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA99550.13985324330277868No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT90000.1264368849547974No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86830.12198349689583399No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC82930.11650456521445944No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC79360.1114892354445858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA125300.0177.802744
CTATCAT53650.0134.1043244
TTAAGCC121700.0104.4223843
TCGCAAA7900.073.2205443
ATTAAGC169900.071.7013542
CGACGGG44500.070.5887744
GAGTTAC5650.068.2527843
ATTAGTC5250.067.1386644
TCCGCAC1950.065.9193543
ATGTGCG351.6650847E-765.2610342
ATCTACG1600.064.7937444
CGTACAA2800.062.94248644
TTACGAG1650.062.8302944
GATATCC1050.061.21082743
AACGACA4700.059.67167743
AGGCGTA1500.058.43702741
TTACGAT806.7484507E-1058.4285243
ATAACGG603.5768244E-758.4285243
CGTATCC1100.058.42851643
ACGAAAC909.038662E-657.59443344
Produced by FastQC (version 0.11.5)