##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840939.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7118176
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.41641201341467	39.0	37.0	39.0	35.0	39.0
2	37.831023565587586	39.0	38.0	39.0	35.0	39.0
3	38.019987002288225	39.0	38.0	39.0	35.0	40.0
4	38.50734542107416	39.0	38.0	41.0	35.0	41.0
5	39.27416883201539	41.0	39.0	41.0	35.0	41.0
6	39.25392965838439	40.0	39.0	41.0	35.0	41.0
7	39.27016036130604	40.0	39.0	41.0	35.0	41.0
8	39.14412048817	40.0	38.0	41.0	35.0	41.0
9	39.06890655696066	40.0	38.0	41.0	35.0	41.0
10	39.03104994875092	40.0	38.0	41.0	35.0	41.0
11	38.8823360085505	40.0	38.0	41.0	35.0	41.0
12	38.90897766506476	40.0	38.0	41.0	35.0	41.0
13	38.82464426279991	40.0	38.0	41.0	35.0	41.0
14	38.84469799566631	40.0	38.0	41.0	35.0	41.0
15	38.842860867727914	40.0	38.0	41.0	35.0	41.0
16	38.81782889324456	40.0	38.0	41.0	35.0	41.0
17	38.80027256982688	40.0	38.0	41.0	35.0	41.0
18	38.80675836056877	40.0	38.0	41.0	35.0	41.0
19	38.827474060770626	40.0	38.0	41.0	35.0	41.0
20	38.83780774737798	40.0	38.0	41.0	35.0	41.0
21	38.77777818362457	40.0	38.0	41.0	35.0	41.0
22	38.70205527371057	40.0	38.0	41.0	35.0	41.0
23	38.5947617760505	40.0	37.0	41.0	35.0	41.0
24	38.4196542766012	40.0	37.0	41.0	35.0	41.0
25	38.34205011367624	40.0	37.0	41.0	34.0	41.0
26	38.30677716444295	40.0	36.0	41.0	34.0	41.0
27	38.201947595880625	40.0	36.0	41.0	34.0	41.0
28	38.13090808639364	40.0	36.0	41.0	34.0	41.0
29	38.02126625209545	40.0	35.0	41.0	34.0	41.0
30	37.92915679272071	40.0	35.0	41.0	33.0	41.0
31	37.85107443083621	40.0	35.0	41.0	33.0	41.0
32	37.746216994644165	39.0	35.0	41.0	33.0	41.0
33	37.61293385885396	39.0	35.0	41.0	33.0	41.0
34	37.50217965965351	39.0	35.0	41.0	33.0	41.0
35	37.390461213750456	39.0	35.0	41.0	33.0	41.0
36	37.329979082041234	38.0	35.0	41.0	33.0	41.0
37	37.23650412236708	38.0	35.0	41.0	33.0	41.0
38	37.1304130016331	38.0	35.0	41.0	33.0	41.0
39	37.02941316895494	38.0	35.0	41.0	33.0	41.0
40	36.922495626765276	37.0	35.0	40.0	33.0	41.0
41	36.813956500375504	37.0	35.0	40.0	33.0	41.0
42	36.7135380078968	37.0	35.0	40.0	33.0	41.0
43	36.59430585808637	36.0	35.0	40.0	33.0	41.0
44	36.456500876390436	36.0	35.0	40.0	33.0	41.0
45	36.286018502129515	36.0	35.0	40.0	32.0	41.0
46	36.05812336022151	36.0	35.0	40.0	31.0	41.0
47	35.91564128761038	35.0	35.0	40.0	31.0	41.0
48	35.86248348151326	36.0	34.0	40.0	31.0	41.0
49	36.07087722237176	36.0	34.0	40.0	31.0	41.0
50	36.13024368140167	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	17.0
21	67.0
22	154.0
23	339.0
24	730.0
25	1707.0
26	3087.0
27	6037.0
28	12176.0
29	23285.0
30	43777.0
31	78200.0
32	132679.0
33	235030.0
34	478243.0
35	409000.0
36	591080.0
37	913773.0
38	1134088.0
39	1412166.0
40	1642537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.064403015604	21.33186928786251	35.6037276965335
2	28.31354549255315	21.367594732133625	28.250959234500524	22.0679005408127
3	26.289979343022708	17.42987248418696	26.478047185121582	29.80210098766875
4	22.410530450497433	20.653394914652292	30.139504839442015	26.79656979540826
5	24.289705677409493	18.903761300647805	31.99840801913299	24.808125002809707
6	24.357363459403082	23.26289768614881	25.91300074625859	26.466738108189514
7	33.24178834577847	18.50082099683964	20.23706634958169	28.0203243078002
8	25.966146383567924	20.40164502816452	26.390229744249087	27.241978844018465
9	25.29700024275882	23.387789231398607	27.189507536762225	24.12570298908035
10	29.66110981240138	19.149596750628252	22.128070449508414	29.061222987461953
11	27.03876667281056	22.606957175546093	24.73038036710528	25.62389578453806
12	26.603683303138332	19.846994510953365	25.593466640892277	27.95585554501603
13	26.64400262089614	20.38794769896108	24.02408987920501	28.94395980093777
14	23.876917345117626	20.117569444756633	22.86366619763265	33.14184701249309
15	24.233061952949743	23.501203117203058	26.0298424764996	26.2358924533476
16	28.50461972280539	22.178546863690922	21.32549124944368	27.991342164060008
17	27.952090535552927	25.07402177186965	18.347509249560563	28.626378443016865
18	28.623006792751404	21.409908942965163	18.28146704998584	31.685617214297597
19	28.468683550392686	24.248810369398	20.879815278520788	26.40269080168852
20	30.688549988086837	20.31259131552802	21.632648588627198	27.366210107757944
21	28.94027908273131	23.260888744532306	21.8303958766965	25.968436296039883
22	28.337723034665057	24.503960003236784	21.885199242053023	25.273117720045136
23	32.91715461938564	21.87242911667259	20.430936239845714	24.77948002409606
24	29.589476292803102	22.91556151463521	21.630737986810104	25.864224205751583
25	28.43169770179193	25.88797101559913	21.373097538197676	24.30723374441126
26	29.954518429131383	22.967163779805524	23.657147709527415	23.42117008153568
27	26.701914582155332	26.858355431613457	20.980956856123615	25.45877313010759
28	27.326681573314904	22.93751666543988	22.827735989072913	26.9080657721723
29	31.089518760342404	23.887716260114072	21.25652906517235	23.76623591437117
30	27.418684314830305	25.060602442484413	20.6618697382119	26.858843504473377
31	26.681489740725933	24.33122265964054	24.301525200508674	24.685762399124847
32	26.02807540127164	22.75590150003409	23.918237769804602	27.297785328889674
33	27.876881385596054	22.525462991550857	22.49474979254991	27.102905830303182
34	25.389387538623158	25.20253000459568	25.35049645627	24.057586000511158
35	25.307670159980155	22.683535779743384	21.557419568226692	30.45137449204977
36	24.53416993220407	23.014489039354018	28.079066478963604	24.372274549478306
37	24.514901647462537	24.974223492552213	22.44395024583074	28.06692461415451
38	26.667749774386053	25.248250700654147	22.172079090575338	25.911920434384474
39	26.65226552047609	27.175544889424568	20.17325027513697	25.99893931496237
40	26.71905047650997	27.61595035340248	20.58890099929385	25.0760981707937
41	29.18462219802405	23.67656442154621	20.555593578331642	26.5832198020981
42	26.820235674281722	27.31909651342776	20.772339780930725	25.08832803135979
43	27.160215810755183	22.91713386725902	24.37534630105188	25.547304020933918
44	26.741144105204334	22.70411035611292	23.447323114363037	27.10742242431971
45	26.99372228938936	24.731889789895195	23.300596437508396	24.97379148320705
46	26.460268803314047	27.73161050090352	21.003626209701277	24.804494486081154
47	30.330311290061278	22.545228437305482	21.46919533861832	25.65526493401492
48	24.277182401591837	22.98312235501429	20.617172418029877	32.122522825363994
49	26.475244996588632	24.754962751410698	19.719999251856738	29.049793000143936
50	23.176417279226662	32.46443316023419	22.889580965806925	21.469568594732223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90628.0
1	72448.0
2	53620.0
3	47924.0
4	37255.0
5	26598.0
6	18474.0
7	15773.0
8	16650.0
9	17944.0
10	18749.5
11	18262.5
12	16882.0
13	15090.5
14	14724.0
15	16236.0
16	17868.5
17	18584.0
18	19180.5
19	22050.0
20	26623.0
21	30225.0
22	31990.5
23	36720.5
24	41924.5
25	43268.0
26	46534.0
27	53849.5
28	60001.0
29	66421.0
30	75493.5
31	86384.0
32	98835.5
33	108553.0
34	120834.0
35	130302.5
36	135416.0
37	142710.0
38	147860.5
39	165969.5
40	211444.0
41	260171.5
42	265265.5
43	316898.5
44	383715.0
45	323746.5
46	273598.0
47	286478.5
48	311336.0
49	357696.0
50	397330.0
51	411636.5
52	407315.5
53	402272.5
54	411106.5
55	409803.0
56	408404.0
57	422552.0
58	425483.5
59	431472.5
60	437445.0
61	431125.0
62	420382.0
63	419662.5
64	418087.0
65	402370.5
66	383902.0
67	373358.0
68	366553.0
69	351743.5
70	311734.0
71	275963.5
72	272321.0
73	256140.5
74	210396.5
75	172650.0
76	143977.0
77	108793.5
78	90089.5
79	85477.0
80	69779.0
81	44397.5
82	29205.5
83	25262.5
84	22741.0
85	19162.5
86	14024.0
87	9334.5
88	4932.0
89	2092.5
90	1233.0
91	815.5
92	654.0
93	550.5
94	399.5
95	203.5
96	115.0
97	109.0
98	55.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	44578.0
25	31405.0
26	29958.0
27	29221.0
28	30449.0
29	34527.0
30	31927.0
31	36200.0
32	48275.0
33	36589.0
34	40963.0
35	28402.0
36	25849.0
37	34605.0
38	37605.0
39	36690.0
40	44371.0
41	43815.0
42	69359.0
43	106537.0
44	296893.0
45	557912.0
46	868209.0
47	813656.0
48	1300759.0
49	1069082.0
50	1390340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.926257056202225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85537115693715	16.501477670923016
2	9.959678460629409	4.16837583328503
3	3.1223532388744304	1.9601749949085572
4	1.4093179681976034	1.1796700030571072
5	0.8152242738365938	0.8529796356380179
6	0.5542189894294031	0.6958637422936986
7	0.4068600245516552	0.5959840221762479
8	0.296290134460503	0.4960194813549766
9	0.23819639321962857	0.44861030589367545
>10	2.410171788115152	12.092579812384923
>50	1.1712008570943913	17.749783482962602
>100	0.7061769521476152	25.105491170690424
>500	0.0337231875548016	4.699775669143548
>1k	0.018536586536745253	7.43761161363319
>5k	0.0022333236791259336	3.494804446638744
>10k+	4.4666473582518674E-4	2.5207981150162557
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	37992	0.5337322370225182	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15946	0.22401806305435548	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	9955	0.13985324330277868	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	9000	0.1264368849547974	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8683	0.12198349689583399	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	8293	0.11650456521445944	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	7936	0.1114892354445858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.018810998772719304	0.0	0.0	0.0	0.0
2	0.03670884226520951	0.0	0.0	0.0	0.0
3	0.048074113368368525	0.0	0.0	0.0	0.0
4	0.060015374725210506	0.0	0.0	0.0	0.0
5	0.06951218963959306	0.0	0.0	0.0	0.0
6	0.08592088759817122	0.0	0.0	0.0	0.0
7	0.09644324613496491	0.0	0.0	0.0	0.0
8	0.10720442989889545	0.0	0.0	0.0	0.0
9	0.11956714753892009	0.0	0.0	0.0	0.0
10	0.13100266135594288	0.0	0.0	0.0	0.0
11	0.14170765094878238	0.0	0.0	0.0	0.0
12	0.15298863079530486	0.0	0.0	0.0	0.0
13	0.1650422804943289	0.0	0.0	0.0	0.0
14	0.17550844486003156	0.0	0.0	0.0	0.0
15	0.18966937597496886	0.0	0.0	0.0	5.619417109102107E-5
16	0.20038841411058114	0.0	0.0	0.0	5.619417109102107E-5
17	0.21476007336710978	0.0	0.0	0.0	5.619417109102107E-5
18	0.23379584882419316	0.0	0.0	0.0	5.619417109102107E-5
19	0.2463131004347181	0.0	0.0	0.0	5.619417109102107E-5
20	0.2591675170717892	0.0	0.0	0.0	5.619417109102107E-5
21	0.27116497259972216	0.0	0.0	0.0	5.619417109102107E-5
22	0.28425821446393007	0.0	0.0	0.0	5.619417109102107E-5
23	0.29673332044613676	0.0	0.0	0.0	5.619417109102107E-5
24	0.3102058729652091	0.0	0.0	0.0	5.619417109102107E-5
25	0.324099881767464	0.0	0.0	0.0	5.619417109102107E-5
26	0.33941279338976726	0.0	0.0	0.0	5.619417109102107E-5
27	0.3548240448114798	0.0	0.0	0.0	5.619417109102107E-5
28	0.36749583039250505	0.0	0.0	0.0	5.619417109102107E-5
29	0.38252777115935316	0.0	0.0	0.0	5.619417109102107E-5
30	0.3965341683037902	0.0	0.0	0.0	5.619417109102107E-5
31	0.4098100412240439	0.0	0.0	0.0	5.619417109102107E-5
32	0.4234511762563893	0.0	0.0	0.0	5.619417109102107E-5
33	0.43783688405569066	0.0	0.0	0.0	5.619417109102107E-5
34	0.4513937278313995	0.0	0.0	0.0	5.619417109102107E-5
35	0.46305401833278637	0.0	0.0	0.0	5.619417109102107E-5
36	0.4734780370701708	0.0	0.0	0.0	5.619417109102107E-5
37	0.48117663850964065	0.0	0.0	0.0	5.619417109102107E-5
38	0.4870629779314251	0.0	0.0	0.0	5.619417109102107E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	12530	0.0	177.8027	44
CTATCAT	5365	0.0	134.10432	44
TTAAGCC	12170	0.0	104.42238	43
TCGCAAA	790	0.0	73.22054	43
ATTAAGC	16990	0.0	71.70135	42
CGACGGG	4450	0.0	70.58877	44
GAGTTAC	565	0.0	68.25278	43
ATTAGTC	525	0.0	67.13866	44
TCCGCAC	195	0.0	65.91935	43
ATGTGCG	35	1.6650847E-7	65.26103	42
ATCTACG	160	0.0	64.79374	44
CGTACAA	280	0.0	62.942486	44
TTACGAG	165	0.0	62.83029	44
GATATCC	105	0.0	61.210827	43
AACGACA	470	0.0	59.671677	43
AGGCGTA	150	0.0	58.437027	41
TTACGAT	80	6.7484507E-10	58.42852	43
ATAACGG	60	3.5768244E-7	58.42852	43
CGTATCC	110	0.0	58.428516	43
ACGAAAC	90	9.038662E-6	57.594433	44
>>END_MODULE