FastQCFastQC Report
Tue 24 May 2016
ERR840938.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840938.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7825767
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCAC286830.36651998455870205No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA114690.14655432496265222No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG104800.13391658606753817No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87150.1113628862193316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT44800.0146.7655844
CACTGCA187850.099.6283344
GAACGTA350.001299043380.1678344
CGAAACG251.8596565E-675.1812142
CCTATCA56050.074.26208543
CGGATTA1101.7462298E-1068.0211944
AGACGAT3000.066.61346443
GCACTGC192450.064.2622243
TTAAGCC17250.063.22072643
TTAGCGA255.315269E-662.9939541
GACGATC5200.062.95230544
TAAGCCA28500.062.6809344
CTCGGTA1900.059.0710344
CGAAATA1307.712515E-1057.55639644
CCCTATC76750.056.1287842
CCCATAC6400.055.53292544
GACCGTA1556.002665E-1154.3072444
AACGACA4900.052.43625343
CTAATCG2900.050.55288742
CTATTCG751.0506356E-449.88220644
Produced by FastQC (version 0.11.5)