##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840938.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7825767
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.53881568413678	39.0	38.0	39.0	35.0	39.0
2	37.90311569460221	39.0	38.0	39.0	35.0	39.0
3	38.048292518803585	39.0	38.0	39.0	35.0	39.0
4	38.43858269738928	39.0	38.0	40.0	35.0	41.0
5	39.245635986862375	40.0	39.0	41.0	35.0	41.0
6	39.31279042680417	41.0	39.0	41.0	35.0	41.0
7	39.14979528524169	40.0	38.0	41.0	35.0	41.0
8	39.2019693405132	40.0	39.0	41.0	35.0	41.0
9	39.15563113494179	40.0	38.0	41.0	35.0	41.0
10	38.836043674696676	40.0	38.0	41.0	35.0	41.0
11	38.84004903289352	40.0	38.0	41.0	35.0	41.0
12	38.90188092745414	40.0	38.0	41.0	35.0	41.0
13	38.79295460751643	40.0	38.0	41.0	35.0	41.0
14	38.801820447759304	40.0	38.0	41.0	35.0	41.0
15	38.88283205978404	40.0	38.0	41.0	35.0	41.0
16	38.79486240773588	40.0	38.0	41.0	35.0	41.0
17	38.77181482658505	40.0	38.0	41.0	35.0	41.0
18	38.82899848666591	40.0	38.0	41.0	35.0	41.0
19	38.88142235770628	40.0	38.0	41.0	35.0	41.0
20	38.89957380535352	40.0	38.0	41.0	35.0	41.0
21	38.81830931076788	40.0	38.0	41.0	35.0	41.0
22	38.711299480293754	40.0	38.0	41.0	35.0	41.0
23	38.39942027407665	40.0	37.0	41.0	34.0	41.0
24	38.3705161423794	40.0	37.0	41.0	34.0	41.0
25	38.368647262943654	40.0	37.0	41.0	34.0	41.0
26	38.352083784569196	40.0	37.0	41.0	34.0	41.0
27	38.3029524983723	40.0	37.0	41.0	34.0	41.0
28	38.11756682267039	40.0	36.0	41.0	34.0	41.0
29	38.08397390268005	40.0	36.0	41.0	34.0	41.0
30	38.01799021693374	40.0	35.0	41.0	33.0	41.0
31	37.92487382158635	40.0	35.0	41.0	33.0	41.0
32	37.87621248563984	40.0	35.0	41.0	33.0	41.0
33	37.77647065003382	39.0	35.0	41.0	33.0	41.0
34	37.65978156943295	39.0	35.0	41.0	33.0	41.0
35	37.575625622477254	39.0	35.0	41.0	33.0	41.0
36	37.47446880673324	39.0	35.0	41.0	33.0	41.0
37	37.41352670489085	39.0	35.0	41.0	33.0	41.0
38	37.351912582249525	39.0	35.0	41.0	33.0	41.0
39	37.255841200761616	39.0	35.0	41.0	33.0	41.0
40	37.216800589477614	38.0	35.0	41.0	33.0	41.0
41	37.12394720194957	38.0	35.0	41.0	33.0	41.0
42	36.99249778151391	38.0	35.0	40.0	33.0	41.0
43	36.90692994818632	37.0	35.0	40.0	33.0	41.0
44	36.78415597742432	37.0	35.0	40.0	33.0	41.0
45	36.67931575005252	37.0	35.0	40.0	32.0	41.0
46	36.41538847761711	37.0	35.0	40.0	31.0	41.0
47	36.282021904419196	37.0	35.0	40.0	31.0	41.0
48	36.17149572452145	37.0	35.0	40.0	31.0	41.0
49	36.465238587162155	37.0	35.0	40.0	32.0	41.0
50	36.28075807713566	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	18.0
21	59.0
22	147.0
23	339.0
24	719.0
25	1718.0
26	3147.0
27	6534.0
28	13320.0
29	26392.0
30	49033.0
31	87284.0
32	143287.0
33	244328.0
34	491923.0
35	411121.0
36	622584.0
37	982552.0
38	1196303.0
39	1656094.0
40	1888856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.71662202567493	15.524471914382321	34.75890605994275
2	27.197168021997076	23.832207117845446	30.32935940975498	18.641265450402496
3	29.706570614739743	15.985883556206057	25.023106361331738	29.28443946772246
4	21.53542266208539	18.237202819864173	31.24418347747895	28.983191040571487
5	20.67137955934543	25.65435439107758	26.070748081306277	27.60351796827071
6	26.08682318295446	24.04453135392352	24.1043849120476	25.76426055107442
7	28.322846821276432	23.536836708785223	24.846075790398565	23.294240679539783
8	30.32216522674391	21.871287504470807	24.286245680455348	23.520301588329936
9	32.2828164958144	22.54801350461878	23.392569699557882	21.776600300008933
10	28.89320625058221	19.464264652908785	27.00105944886936	24.64146964763965
11	28.30553222450911	24.794822028307255	24.345179200965223	22.55446654621841
12	27.992783327180582	21.126823734977034	26.490694138989827	24.38969879885256
13	25.12653392312856	20.72011599629787	24.71195475152787	29.4413953290457
14	22.136756691069387	27.23899139854279	22.88033875784955	27.743913152538273
15	22.974936514210047	27.84421003078676	23.41615844172207	25.764695013281123
16	24.140534723305716	27.009620910001537	19.97477052409048	28.875073842602266
17	30.765240007784538	26.859488660983644	18.914542178421616	23.4607291528102
18	25.03533519462054	22.018927984950228	20.231256565650373	32.71448025477886
19	26.81119946453811	26.256467896373607	20.997111209674397	25.935221429413886
20	31.049442182472337	21.328363085688597	22.26804094729628	25.35415378454278
21	26.10669343976124	28.470666197958614	20.36016405804057	25.062476304239574
22	31.25669343337209	23.013731433609	22.85855175601318	22.87102337700573
23	27.799409310295083	22.856596676082997	26.852716161879087	22.49127785174284
24	31.055946337272754	23.655547117617992	22.40460264150466	22.88390390360459
25	26.68062339480139	25.089873098142597	20.85055876908198	27.378944737974038
26	27.87509377295804	29.248286985749615	21.16292959271419	21.713689648578157
27	29.38880371954221	24.78958104087466	21.919432526582778	23.90218271300035
28	26.776037817174224	22.875230422438197	25.568963284881637	24.779768475505943
29	30.70638492167691	25.57064735870968	20.33172951818948	23.391238201423928
30	26.75163921769812	24.97657788696283	21.461497586066113	26.810285309272935
31	25.132276749106243	25.290820184932354	24.947719063104355	24.62918400285705
32	25.1520690330389	25.334863301938682	21.985929546148668	27.52713811887375
33	24.301158891918195	22.762362910071055	26.350184106525067	26.586294091485684
34	24.271218134811804	24.968167681026745	23.725060391269825	27.035553792891626
35	25.005181767111534	24.134986022532875	21.12131939320859	29.738512817147
36	24.237455848229867	27.914085429970736	24.327666503487137	23.520792218312256
37	25.01399757504079	26.81954684815982	26.199185354923337	21.967270221876046
38	29.52340485015637	25.225419998158365	21.27726257609225	23.973912575593012
39	28.566226987929333	24.861706289316782	22.18089830948137	24.39116841327251
40	28.94555871993689	25.60843955298135	21.977157461650528	23.46884426543123
41	28.6007837343285	25.52525625621801	20.9856400284737	24.888319980979794
42	24.416364214963888	29.19640331506837	22.747452447976787	23.63978002199096
43	28.602801830231765	23.783305935820152	22.158551911256	25.455340322692077
44	24.451205746536687	26.062640181778203	21.94646338781793	27.539690683867185
45	24.994227844876317	26.99166911128409	23.550515390678445	24.463587653161152
46	24.538324681092945	25.466150470898498	22.69645959223221	27.299065255776345
47	26.36752183040423	24.38093197883934	25.622618332685455	23.62892785807098
48	27.19423620059167	25.54606513070352	21.620303801577005	25.639394867127802
49	25.042848946437175	26.0718068877631	21.300428161399278	27.584916004400444
50	21.26386865068826	34.1156843462802	23.36752492588631	21.25292207714523
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79498.0
1	67094.5
2	62425.5
3	68315.5
4	60744.5
5	47394.5
6	36780.5
7	32657.0
8	32034.0
9	32103.0
10	30534.5
11	29832.5
12	30418.5
13	30501.0
14	30611.0
15	32018.0
16	34091.5
17	35105.5
18	36851.5
19	40700.5
20	44939.0
21	50016.5
22	54632.5
23	62134.0
24	71202.5
25	77461.0
26	84045.0
27	91502.5
28	96509.5
29	103254.5
30	114744.5
31	128964.0
32	147181.0
33	163667.5
34	174565.0
35	174697.5
36	179240.0
37	196729.0
38	208184.0
39	214953.5
40	223467.5
41	231614.0
42	237295.5
43	246646.0
44	260106.0
45	266594.0
46	274390.0
47	292736.5
48	328688.0
49	374554.5
50	429722.5
51	483530.0
52	508636.0
53	536687.0
54	578014.5
55	638494.5
56	667754.0
57	585652.0
58	490275.0
59	452418.0
60	438534.5
61	433027.5
62	425062.5
63	407786.0
64	378995.0
65	363853.0
66	360225.0
67	351602.5
68	345271.5
69	331521.5
70	297587.0
71	262947.0
72	242302.5
73	228792.5
74	203928.0
75	169632.5
76	138252.5
77	104421.0
78	83062.0
79	72302.0
80	57476.0
81	39639.0
82	27114.0
83	22142.0
84	18248.0
85	14898.5
86	13407.0
87	11579.0
88	7615.5
89	4652.5
90	2532.0
91	1200.5
92	1179.0
93	1117.5
94	659.0
95	168.5
96	54.0
97	52.0
98	140.0
99	228.0
100	228.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	58426.0
25	38567.0
26	32343.0
27	42554.0
28	40988.0
29	42052.0
30	44193.0
31	45009.0
32	57129.0
33	46784.0
34	49141.0
35	40085.0
36	37240.0
37	40272.0
38	48330.0
39	43130.0
40	53354.0
41	52378.0
42	80681.0
43	111611.0
44	290144.0
45	576251.0
46	969627.0
47	807817.0
48	1434303.0
49	1071602.0
50	1671756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.320213008694253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67840984385047	14.621229983424552
2	11.170723766597094	4.316415252638784
3	3.66370748838111	2.123508272312138
4	1.7157874165262044	1.325975134596939
5	0.9781543485335167	0.944907518452405
6	0.6508443188292236	0.7544670525167468
7	0.44953855293943956	0.6079626418887106
8	0.3631396868668054	0.5612748881741769
9	0.2605160125707306	0.4529902369537966
>10	2.9002165255328696	13.516528093984654
>50	1.3001649013225967	18.26977764085968
>100	0.8117340041992372	26.129762991600774
>500	0.033758724224639616	4.340667516845078
>1k	0.021562023859608526	8.46723667369105
>5k	0.0010889911040206327	1.4367481139562632
>10k+	6.533946624123797E-4	2.1305479881043086
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAGCAC	28683	0.36651998455870205	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	11469	0.14655432496265222	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	10480	0.13391658606753817	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8715	0.1113628862193316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05250603551064068	0.0	0.0	0.0	0.0
2	0.09696174189699233	0.0	0.0	0.0	0.0
3	0.10804052816803772	0.0	0.0	0.0	0.0
4	0.13046644501427143	0.0	0.0	0.0	0.0
5	0.19466462520542716	0.0	0.0	0.0	0.0
6	0.2310572241672925	0.0	0.0	0.0	0.0
7	0.24911296234605504	0.0	0.0	0.0	0.0
8	0.26493249799029284	0.0	0.0	0.0	0.0
9	0.2830010144692527	0.0	0.0	0.0	0.0
10	0.2966226824795576	0.0	0.0	0.0	0.0
11	0.31497232156285765	0.0	0.0	0.0	0.0
12	0.3291690130820404	0.0	0.0	0.0	0.0
13	0.34478409592312165	0.0	0.0	0.0	0.0
14	0.3588530044403315	0.0	0.0	0.0	0.0
15	0.38000109126683684	0.0	0.0	0.0	0.0
16	0.39824850394855865	0.0	0.0	0.0	0.0
17	0.42634798608238655	0.0	0.0	0.0	0.0
18	0.4519045864769549	0.0	0.0	0.0	0.0
19	0.5068001641244877	0.0	0.0	0.0	0.0
20	0.5232458364783925	0.0	0.0	0.0	0.0
21	0.5541565446556229	0.0	0.0	0.0	0.0
22	0.5692093822880236	0.0	0.0	0.0	0.0
23	0.5863323045523845	0.0	0.0	0.0	0.0
24	0.6010656846798531	0.0	0.0	0.0	0.0
25	0.6195175501647314	0.0	0.0	0.0	0.0
26	0.67970334409394	0.0	0.0	0.0	0.0
27	0.6950500826308782	0.0	0.0	0.0	0.0
28	0.7147925564356823	0.0	0.0	0.0	0.0
29	0.7319410353004376	0.0	0.0	0.0	0.0
30	0.7486678302586827	0.0	0.0	0.0	0.0
31	0.7634012103861513	0.0	0.0	0.0	0.0
32	0.7781984820146064	0.0	0.0	0.0	0.0
33	0.7914751359195846	0.0	0.0	0.0	0.0
34	0.8055568227369918	0.0	0.0	0.0	0.0
35	0.8175684249224389	0.0	0.0	0.0	0.0
36	0.8290050035990083	0.0	0.0	0.0	0.0
37	0.8386781768483523	0.0	0.0	0.0	0.0
38	0.844773426042457	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	4480	0.0	146.76558	44
CACTGCA	18785	0.0	99.62833	44
GAACGTA	35	0.0012990433	80.16783	44
CGAAACG	25	1.8596565E-6	75.18121	42
CCTATCA	5605	0.0	74.262085	43
CGGATTA	110	1.7462298E-10	68.02119	44
AGACGAT	300	0.0	66.613464	43
GCACTGC	19245	0.0	64.26222	43
TTAAGCC	1725	0.0	63.220726	43
TTAGCGA	25	5.315269E-6	62.99395	41
GACGATC	520	0.0	62.952305	44
TAAGCCA	2850	0.0	62.68093	44
CTCGGTA	190	0.0	59.07103	44
CGAAATA	130	7.712515E-10	57.556396	44
CCCTATC	7675	0.0	56.12878	42
CCCATAC	640	0.0	55.532925	44
GACCGTA	155	6.002665E-11	54.30724	44
AACGACA	490	0.0	52.436253	43
CTAATCG	290	0.0	50.552887	42
CTATTCG	75	1.0506356E-4	49.882206	44
>>END_MODULE