FastQCFastQC Report
Tue 24 May 2016
ERR840937.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840937.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9679102
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC289340.29893269024337177No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC227740.2352904226032539No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG205120.21192048601202879No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG113770.11754189593208131No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA107340.11089871767029627No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100090.10340835337823695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT32950.0129.9663244
TAAGCCA40900.091.2174644
ACGGTAT402.1590658E-673.31547543
CACGATT2950.071.39300544
TATGCGC850.071.3637242
TTAAGCC34900.063.8621943
CCTATCA42750.058.85817743
AATCGCA4450.056.34279344
TTATACG909.813393E-755.7167644
ATTAAGC38650.055.12685842
ATAGTCG1107.2759576E-1254.70372844
GTGTTAG22950.054.43554343
AGGCCGA116850.052.98267742
CCCTATC49550.051.72249242
CGAGATT9050.050.9762244
TACGCTA15450.050.79468542
TGTGTTA28500.050.41627542
CAACTAG3000.050.1450944
ACGACAC5500.049.23335644
TCGCAAA10950.047.671843
Produced by FastQC (version 0.11.5)