##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840937.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9679102
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.56668407875028	39.0	38.0	39.0	35.0	39.0
2	37.92510524220119	39.0	38.0	39.0	35.0	39.0
3	38.09146922927354	39.0	38.0	39.0	35.0	40.0
4	38.55274931496744	39.0	38.0	41.0	35.0	41.0
5	39.3193253878304	41.0	39.0	41.0	35.0	41.0
6	39.321098486202544	41.0	39.0	41.0	35.0	41.0
7	39.352159425533486	41.0	39.0	41.0	35.0	41.0
8	39.26941104660329	40.0	39.0	41.0	35.0	41.0
9	39.17119429054472	40.0	39.0	41.0	35.0	41.0
10	39.110753042999235	40.0	39.0	41.0	35.0	41.0
11	38.84170225708955	40.0	38.0	41.0	35.0	41.0
12	38.91982913290923	40.0	38.0	41.0	35.0	41.0
13	38.86341088253848	40.0	38.0	41.0	35.0	41.0
14	38.84681647119743	40.0	38.0	41.0	35.0	41.0
15	38.88178438454311	40.0	38.0	41.0	35.0	41.0
16	38.811900525482635	40.0	38.0	41.0	35.0	41.0
17	38.7914226960311	40.0	38.0	41.0	35.0	41.0
18	38.81132020305189	40.0	38.0	41.0	35.0	41.0
19	38.86235437956951	40.0	38.0	41.0	35.0	41.0
20	38.891501918256466	40.0	38.0	41.0	35.0	41.0
21	38.818101204016656	40.0	38.0	41.0	35.0	41.0
22	38.70125617025216	40.0	38.0	41.0	35.0	41.0
23	38.60512679791989	40.0	37.0	41.0	35.0	41.0
24	38.44802183095084	40.0	37.0	41.0	34.0	41.0
25	38.40077359507632	40.0	37.0	41.0	34.0	41.0
26	38.343067703300555	40.0	37.0	41.0	34.0	41.0
27	38.27529237120324	40.0	36.0	41.0	34.0	41.0
28	38.16275723265139	40.0	36.0	41.0	34.0	41.0
29	38.05692717652082	40.0	36.0	41.0	34.0	41.0
30	37.956432593698594	40.0	35.0	41.0	34.0	41.0
31	37.82113727171716	40.0	35.0	41.0	33.0	41.0
32	37.72414219232708	39.0	35.0	41.0	33.0	41.0
33	37.62945772813971	39.0	35.0	41.0	33.0	41.0
34	37.54619494351125	39.0	35.0	41.0	33.0	41.0
35	37.42101838876262	39.0	35.0	41.0	33.0	41.0
36	37.34939201703467	39.0	35.0	41.0	33.0	41.0
37	37.28335228997025	38.0	35.0	41.0	33.0	41.0
38	37.19467835762236	38.0	35.0	41.0	33.0	41.0
39	37.11902230306924	38.0	35.0	41.0	33.0	41.0
40	37.04089862838966	38.0	35.0	41.0	33.0	41.0
41	36.93428303735808	37.0	35.0	40.0	33.0	41.0
42	36.8225301274747	37.0	35.0	40.0	33.0	41.0
43	36.72325134507903	37.0	35.0	40.0	33.0	41.0
44	36.5721488721957	37.0	35.0	40.0	33.0	41.0
45	36.45394131878328	37.0	35.0	40.0	32.0	41.0
46	36.17648136589441	36.0	35.0	40.0	31.0	41.0
47	36.04825971513854	36.0	35.0	40.0	31.0	41.0
48	35.867297185184725	36.0	34.0	40.0	30.0	41.0
49	36.19369450631604	36.0	34.0	40.0	31.0	41.0
50	36.187636513112764	37.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	21.0
21	73.0
22	153.0
23	440.0
24	834.0
25	1892.0
26	3710.0
27	7653.0
28	15402.0
29	30524.0
30	57004.0
31	102509.0
32	172518.0
33	298356.0
34	639160.0
35	516040.0
36	813414.0
37	1279804.0
38	1480446.0
39	1955853.0
40	2303290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	45.60862154361014	16.574926062355786	37.81645239403407
2	28.088814437537696	19.567331762801963	28.760684617229987	23.583169182430353
3	27.309899203459164	17.065860035362785	28.14955354329358	27.47468721788447
4	24.028458425172087	18.55264052388331	31.817218167553147	25.601682883391458
5	24.73552815126858	20.916971429787598	29.157549946265675	25.189950472678145
6	23.162024741551438	22.98783502849748	27.65535480460894	26.194785425342143
7	31.78615123593077	20.90604066368967	22.132590399398623	25.17521770098094
8	26.605835954616452	20.752865296801296	24.445542572027858	28.195756176554394
9	28.656356757062795	20.452010940684374	25.907475714172655	24.984156588080175
10	30.591329650209286	18.628773619701498	24.618885099051543	26.161011631037674
11	28.388966249141706	20.689202366087265	26.12274361815797	24.79908776661306
12	30.638358806426464	20.2190451138959	23.65366125907135	25.48893482060629
13	29.22002475022993	18.987835855020435	26.555624685017264	25.236514709732372
14	24.75841250562294	20.67962503133039	23.070342682616634	31.491619780430042
15	24.688085733573217	22.348984440911977	26.20851603795476	26.754413787560043
16	26.537441179977233	23.799459908574164	21.418474565099118	28.244624346349482
17	27.065248408375076	26.783352422569777	20.020875903570392	26.13052326548475
18	25.815401056833576	20.789697226044318	21.32278386982594	32.07211784729617
19	28.726797176019016	21.309394197932825	22.738721009449016	27.22508761659914
20	28.248271378894447	20.202452665546865	23.105180625227423	28.444095330331265
21	27.456348739790116	23.544374261165963	21.642710243160987	27.356566755882934
22	28.541872996069262	22.365246280078463	24.80520403649016	24.28767668736211
23	31.593034147176052	22.48661084468373	21.993197302807637	23.92715770533258
24	29.57004689071362	22.352796778048212	24.88106851234753	23.196087818890636
25	28.22116821927293	25.752608522070158	21.901682440303006	24.12454081835391
26	29.69785558650902	25.736849216625668	22.500933428203567	22.064361768661744
27	28.48483131774894	23.8581406266802	23.632489594234144	24.02453846133671
28	28.30875821263547	22.40063435529165	22.395797659629395	26.89480977244348
29	28.982303696324006	23.536607009732162	21.57995571052917	25.90113358341466
30	28.975655256556244	23.99977373447101	21.38775978057783	25.63681122839492
31	26.715988852591842	26.14154406192281	22.12441526398399	25.018051821501363
32	28.87664295027617	22.68453824793199	23.670109095108984	24.76870970668286
33	25.550250181305746	22.114471052836652	23.91671852774309	28.418560238114516
34	26.682693252758476	24.911738380415663	24.228196802662218	24.177371564163643
35	25.932460843651935	22.35886803581661	22.56009766127581	29.148573459255644
36	26.985284787250173	22.805551121901228	26.901190277755056	23.30797381309354
37	26.122860105127444	24.055977803245764	26.221675947331235	23.599486144295557
38	26.364379783915076	24.167243895318382	24.610088653832797	24.858287666933744
39	28.79446231422678	23.875352845629692	22.132918888745163	25.19726595139836
40	27.6411796335173	24.59489181302586	22.350209283798783	25.413719269658063
41	28.34856604738064	23.4000752637763	22.187471952625735	26.063886736217324
42	24.843205910630754	26.472226472627934	23.628460349602815	25.056107267138493
43	28.12598314530193	23.12479510860534	22.74870524873337	26.00051649735936
44	27.880575746165814	23.813895137505856	23.09923437708879	25.20629473923954
45	26.54285552352384	22.615372514484346	23.575727125192827	27.266044836798986
46	26.368834758854387	23.815586556414246	22.832956239007636	26.98262244572373
47	29.17530015444919	22.515785948550125	23.344907863849386	24.9640060331513
48	23.821952309379697	26.4960932251321	22.34915833648417	27.332796129004034
49	26.595146426420545	24.51118417851897	22.257940036980933	26.63572935807955
50	24.14356869544329	28.317174805639496	24.823836930281825	22.71541956863539
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103489.0
1	89996.5
2	86309.0
3	86759.5
4	68293.5
5	48841.5
6	32668.0
7	27534.5
8	28239.0
9	28148.0
10	27519.5
11	28113.0
12	28986.0
13	27490.0
14	26954.0
15	28806.0
16	30090.5
17	30502.5
18	32842.0
19	36386.0
20	39054.5
21	44381.5
22	50316.0
23	57097.5
24	65314.0
25	73130.5
26	83875.5
27	97055.0
28	107361.5
29	116986.0
30	128826.5
31	144170.0
32	164073.0
33	182540.0
34	199816.5
35	209465.0
36	215866.5
37	223405.0
38	231334.0
39	250267.5
40	272881.0
41	307152.5
42	341372.0
43	357405.0
44	361019.5
45	352386.5
46	360695.0
47	383347.5
48	411902.5
49	453947.5
50	510309.0
51	543766.5
52	542150.5
53	541808.0
54	555101.5
55	573333.0
56	582160.5
57	583711.0
58	577204.0
59	571485.5
60	575961.0
61	578125.0
62	564288.5
63	546719.0
64	538086.5
65	539862.0
66	545939.5
67	551003.0
68	570086.0
69	567556.0
70	492731.0
71	395518.5
72	330954.5
73	290981.5
74	259909.5
75	233123.5
76	200151.0
77	155304.5
78	122884.5
79	104222.5
80	81391.0
81	58848.5
82	44981.5
83	36390.5
84	27605.0
85	21907.5
86	17858.5
87	13990.5
88	9118.0
89	5275.0
90	3014.0
91	1527.0
92	955.0
93	832.0
94	629.0
95	336.0
96	173.0
97	111.5
98	68.0
99	20.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	65804.0
25	43735.0
26	37588.0
27	42025.0
28	46622.0
29	56127.0
30	51578.0
31	53997.0
32	67908.0
33	56850.0
34	59301.0
35	50603.0
36	41323.0
37	51939.0
38	55689.0
39	54758.0
40	63467.0
41	61666.0
42	94651.0
43	140705.0
44	384195.0
45	730388.0
46	1181035.0
47	1066812.0
48	1811063.0
49	1377682.0
50	1931591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.340660813605425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37421168831764	13.824128506683275
2	11.328726359056665	4.155526552032188
3	3.718952354374452	2.0462413114062343
4	1.6356246254767202	1.1999374589699572
5	0.9774856702062626	0.896386656370642
6	0.6111764623007782	0.6725628115390726
7	0.4570766956344269	0.5868162048304211
8	0.3491537165239229	0.5122967909260008
9	0.2784745726042558	0.45966669132135535
>10	2.909497848508275	13.112723369409515
>50	1.4339041763509326	19.03154836862216
>100	0.8628966857331253	26.10011624781503
>500	0.040080926815391146	5.07364205086277
>1k	0.020233160171231106	7.02055442346169
>5k	0.0013488773447487407	1.6202829043379698
>10k+	0.0011561805812132062	3.6875696514117644
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	28934	0.29893269024337177	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	22774	0.2352904226032539	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	20512	0.21192048601202879	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAG	11377	0.11754189593208131	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	10734	0.11089871767029627	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10009	0.10340835337823695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01673708986639463	0.0	0.0	0.0	0.0
2	0.03598474321274846	0.0	0.0	0.0	0.0
3	0.048155293745225536	0.0	0.0	0.0	0.0
4	0.05917904367574595	0.0	0.0	0.0	0.0
5	0.06838444310226299	0.0	0.0	0.0	0.0
6	0.08674358427052427	0.0	0.0	0.0	0.0
7	0.10093911604609601	0.0	0.0	0.0	0.0
8	0.11289270430252724	0.0	0.0	0.0	0.0
9	0.12458800413509435	0.0	0.0	0.0	0.0
10	0.1355394333069328	0.0	0.0	0.0	0.0
11	0.14624290559186173	0.0	0.0	0.0	0.0
12	0.15711168246806367	0.0	0.0	0.0	0.0
13	0.16795979627035648	0.0	0.0	0.0	0.0
14	0.1781880178553754	0.0	0.0	0.0	0.0
15	0.1920839350592648	0.0	0.0	0.0	0.0
16	0.2044817794047423	0.0	0.0	0.0	0.0
17	0.2172928852284024	0.0	0.0	0.0	0.0
18	0.2321702984429754	0.0	0.0	0.0	0.0
19	0.244423501271089	0.0	0.0	0.0	0.0
20	0.25503398972342683	0.0	0.0	0.0	0.0
21	0.26786575862099604	0.0	0.0	0.0	0.0
22	0.2799743199317457	0.0	0.0	0.0	0.0
23	0.2923205065924504	0.0	0.0	0.0	0.0
24	0.3051109493422014	0.0	0.0	0.0	0.0
25	0.31838697432881685	0.0	0.0	0.0	0.0
26	0.33498975421480215	0.0	0.0	0.0	0.0
27	0.348637714531782	0.0	0.0	0.0	0.0
28	0.36209970718357964	0.0	0.0	0.0	0.0
29	0.37616092897874204	0.0	0.0	0.0	0.0
30	0.3912553044693609	0.0	0.0	0.0	0.0
31	0.4076101274684366	0.0	0.0	0.0	0.0
32	0.42177466463314467	0.0	0.0	0.0	0.0
33	0.43731329621280984	0.0	0.0	0.0	0.0
34	0.4504756742929251	0.0	0.0	0.0	0.0
35	0.46253257791890195	0.0	0.0	0.0	0.0
36	0.47359765399724063	0.0	0.0	0.0	0.0
37	0.48050945221984437	0.0	0.0	0.0	0.0
38	0.48725594585117504	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	3295	0.0	129.96632	44
TAAGCCA	4090	0.0	91.21746	44
ACGGTAT	40	2.1590658E-6	73.315475	43
CACGATT	295	0.0	71.393005	44
TATGCGC	85	0.0	71.36372	42
TTAAGCC	3490	0.0	63.86219	43
CCTATCA	4275	0.0	58.858177	43
AATCGCA	445	0.0	56.342793	44
TTATACG	90	9.813393E-7	55.71676	44
ATTAAGC	3865	0.0	55.126858	42
ATAGTCG	110	7.2759576E-12	54.703728	44
GTGTTAG	2295	0.0	54.435543	43
AGGCCGA	11685	0.0	52.982677	42
CCCTATC	4955	0.0	51.722492	42
CGAGATT	905	0.0	50.97622	44
TACGCTA	1545	0.0	50.794685	42
TGTGTTA	2850	0.0	50.416275	42
CAACTAG	300	0.0	50.14509	44
ACGACAC	550	0.0	49.233356	44
TCGCAAA	1095	0.0	47.6718	43
>>END_MODULE