##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840936.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8656568
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.31173462739506	39.0	37.0	39.0	34.0	39.0
2	37.84130165673047	39.0	38.0	39.0	35.0	39.0
3	38.02307843015846	39.0	38.0	39.0	35.0	40.0
4	38.57754089149418	39.0	38.0	41.0	35.0	41.0
5	39.254397470221456	40.0	39.0	41.0	35.0	41.0
6	39.22093894485667	40.0	39.0	41.0	35.0	41.0
7	39.244173557003194	40.0	39.0	41.0	35.0	41.0
8	39.171376115800165	40.0	38.0	41.0	35.0	41.0
9	39.062800407736646	40.0	38.0	41.0	35.0	41.0
10	38.98066508574761	40.0	38.0	41.0	35.0	41.0
11	38.74760563308692	40.0	38.0	41.0	35.0	41.0
12	38.78679853262864	40.0	38.0	41.0	35.0	41.0
13	38.69664583007954	40.0	38.0	41.0	35.0	41.0
14	38.65426390689705	40.0	38.0	41.0	35.0	41.0
15	38.593126629398625	40.0	38.0	41.0	35.0	41.0
16	38.55178126019457	40.0	38.0	41.0	35.0	41.0
17	38.51216117057014	40.0	37.0	41.0	35.0	41.0
18	38.565044484141985	40.0	38.0	41.0	35.0	41.0
19	38.639337321672976	40.0	38.0	41.0	35.0	41.0
20	38.62301688151702	40.0	37.0	41.0	35.0	41.0
21	38.54887514312831	40.0	37.0	41.0	35.0	41.0
22	38.44939899969595	40.0	37.0	41.0	35.0	41.0
23	38.321892463618376	40.0	37.0	41.0	34.0	41.0
24	38.12249993299885	40.0	36.0	41.0	34.0	41.0
25	38.05797550637374	40.0	36.0	41.0	34.0	41.0
26	37.99331507561058	40.0	36.0	41.0	33.0	41.0
27	37.91222449235032	40.0	35.0	41.0	33.0	41.0
28	37.801110295467055	39.0	35.0	41.0	33.0	41.0
29	37.7102847230884	39.0	35.0	41.0	33.0	41.0
30	37.619185552334294	39.0	35.0	41.0	33.0	41.0
31	37.45279589373969	39.0	35.0	41.0	33.0	41.0
32	37.33623356741944	39.0	35.0	41.0	33.0	41.0
33	37.189622873322215	38.0	35.0	41.0	33.0	41.0
34	37.11654607796587	38.0	35.0	41.0	33.0	41.0
35	36.96202446233945	38.0	35.0	40.0	33.0	41.0
36	36.88934749178468	37.0	35.0	40.0	33.0	41.0
37	36.80803238408552	37.0	35.0	40.0	33.0	41.0
38	36.75034345955293	37.0	35.0	40.0	33.0	41.0
39	36.635128827791064	37.0	35.0	40.0	33.0	41.0
40	36.53887186478844	36.0	35.0	40.0	33.0	41.0
41	36.43089801839124	36.0	35.0	40.0	33.0	41.0
42	36.30442939154963	36.0	35.0	40.0	32.0	41.0
43	36.185687414445695	35.0	35.0	40.0	32.0	41.0
44	36.05931287909034	35.0	35.0	40.0	32.0	41.0
45	35.85827109802677	35.0	35.0	39.0	31.0	41.0
46	35.58719287282283	35.0	34.0	39.0	31.0	41.0
47	35.39337848557974	35.0	34.0	39.0	30.0	41.0
48	35.39667443954884	35.0	34.0	39.0	30.0	41.0
49	35.45477880194377	35.0	34.0	39.0	31.0	41.0
50	35.85122330951262	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	23.0
21	74.0
22	169.0
23	371.0
24	874.0
25	1953.0
26	3911.0
27	7755.0
28	15855.0
29	31198.0
30	58427.0
31	105480.0
32	177966.0
33	310359.0
34	552839.0
35	536612.0
36	962097.0
37	1340967.0
38	1425239.0
39	1576760.0
40	1547633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.15183569285195	17.059820935964463	39.78834337118359
2	26.05948454399018	21.394714394896454	30.53497644794103	22.010824613172332
3	24.49654412695655	19.372226961077416	28.87709078239783	27.254138129568204
4	23.020682099418615	21.13789206068733	32.62309035174217	23.218335488151887
5	22.54668362796896	22.08681315736213	29.87426425807549	25.49223895659342
6	22.63430495780776	24.17929368775247	26.942444164939268	26.243957189500506
7	29.668339693051564	21.695630416118718	21.39233469892456	27.243695191905154
8	26.505908577163606	21.953088106048494	22.66748207834791	28.87352123843999
9	27.802877537610748	23.017736359259235	24.108688339304905	25.070697763825113
10	29.122753959767888	19.717202013546245	23.752230676175593	27.407813350510274
11	27.401944974035903	21.96576056469492	24.74898828265428	25.883306178614895
12	29.72214854662957	22.42689019482086	22.54775795673297	25.303203301816605
13	28.734043329873916	21.641602076019044	23.814102771444755	25.810251822662284
14	26.454618042623824	22.133806376845882	22.263615326535874	29.147960253994427
15	23.96441638302847	25.972625641016162	24.19131923875605	25.871638737199316
16	27.17768750849066	25.477429392341165	19.531204514306364	27.813678584861805
17	27.002098291147252	28.04761656120532	19.58474767367391	25.36553747397352
18	26.134687557470816	22.862894394175612	19.82521248605683	31.177205562296745
19	29.22530037308088	22.658540890570027	21.205620980508673	26.91053775584042
20	27.466739705620057	22.866094276623254	22.543818751264936	27.123347266491756
21	27.22510814909558	26.798403247106705	19.28734343679851	26.689145166999207
22	28.60507767050406	24.758276028097974	21.63323848435084	25.003407817047123
23	30.975959525761247	25.23653715883708	20.531554768587274	23.255948546814395
24	29.71811692578398	25.06499111426145	22.416193114869543	22.800698845085027
25	28.57595940743518	27.830136230296205	20.513636128127853	23.080268234140764
26	29.347747719865524	28.529456140492787	20.410631120027002	21.712165019614684
27	28.26506492279303	27.441000165039455	21.28795420798109	23.00598070418642
28	27.810493345263716	25.693001615545754	19.898229650922307	26.598275388268227
29	26.779757439427254	27.68462979863861	20.464905567778917	25.07070719415522
30	29.024376796545504	26.938407159509666	20.02634832652096	24.01086771742387
31	26.867870593200056	28.548646555053075	20.70754125153588	23.87594160021099
32	27.644248112838234	25.54830169268409	23.0641127988992	23.743337395578482
33	24.730780860353388	25.223379275064282	23.046755916510325	26.999083948072006
34	27.146324107486347	27.205969772688192	22.050474299426245	23.597231820399216
35	24.644610731883766	25.52944371062555	21.564236185339066	28.261709372151618
36	25.21164131624305	25.73021568536124	25.016539782348772	24.041603216046934
37	23.943749122084732	27.99519173541889	24.273111673628964	23.78794746886742
38	25.149123531284257	25.5110674295306	23.59927634333808	25.74053269584706
39	27.584189385330287	26.605528316998605	21.237758653668205	24.572523644002903
40	26.708602500657147	26.911246942269184	21.131434286502135	25.248716270571535
41	25.93182511676371	26.544017280977112	21.556435488606184	25.96772211365299
42	24.275772564443827	28.902352621786086	21.474040377955674	25.347834435814416
43	26.971768820875415	26.220726626054386	22.00443354875778	24.803071004312418
44	27.396070444062804	27.107540238765665	20.75589543121205	24.740493885959484
45	25.24928978981904	25.6179458853648	23.16613073826334	25.966633586552817
46	24.774263327404704	26.430482782931946	22.13810508099793	26.657148808665415
47	24.939274362664396	26.712587751570886	22.84422096872138	25.503916917043334
48	24.40190813314573	27.785665186218537	20.90356990146113	26.908856779174606
49	24.839560479740005	27.29416033193947	21.08857695928876	26.777702229031764
50	22.378463312215267	30.87593245094614	24.756365967858564	21.98923826898002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256275.0
1	219512.0
2	145097.5
3	100458.5
4	85553.0
5	58516.0
6	30891.0
7	23856.5
8	26380.5
9	28441.5
10	29024.5
11	28214.5
12	26820.5
13	25387.5
14	26122.0
15	28317.5
16	31120.5
17	32581.5
18	32053.0
19	33298.0
20	36762.5
21	44639.5
22	55373.0
23	67432.0
24	76343.5
25	79336.0
26	82180.0
27	86597.0
28	90371.5
29	100054.5
30	116360.0
31	134546.5
32	154048.0
33	161739.5
34	174701.5
35	194807.0
36	200305.0
37	202105.0
38	207885.0
39	218269.5
40	231980.0
41	258316.5
42	289067.0
43	294891.5
44	290414.0
45	293304.0
46	309737.0
47	327619.0
48	350353.0
49	395535.0
50	457975.0
51	501816.5
52	505765.0
53	499925.5
54	506548.0
55	514897.0
56	521951.0
57	531822.0
58	536563.5
59	530859.5
60	528629.5
61	539638.5
62	533588.5
63	522587.5
64	505310.0
65	474435.0
66	446256.5
67	423798.0
68	427868.0
69	426840.0
70	371074.0
71	308008.0
72	271284.0
73	245247.0
74	228382.0
75	210501.0
76	182914.5
77	139145.0
78	102494.0
79	88243.0
80	75368.0
81	55194.5
82	38827.0
83	31622.5
84	27538.5
85	23030.5
86	18632.0
87	14741.0
88	8561.0
89	3857.0
90	2221.0
91	1382.5
92	963.5
93	845.5
94	624.0
95	309.0
96	203.5
97	202.0
98	166.0
99	130.0
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	67803.0
25	58676.0
26	53328.0
27	63505.0
28	67217.0
29	75305.0
30	69403.0
31	65436.0
32	86133.0
33	59240.0
34	61681.0
35	49354.0
36	41316.0
37	50500.0
38	55372.0
39	51358.0
40	64705.0
41	65086.0
42	88054.0
43	126949.0
44	355115.0
45	740132.0
46	951536.0
47	1039975.0
48	1405015.0
49	1453148.0
50	1391226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.777256032967497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90074066386119	13.315296438403603
2	11.415514280389074	4.0587304022094655
3	3.849798577337287	2.0531656495403694
4	1.7991482990539993	1.279356798142437
5	1.0875355108880338	0.9666698611000345
6	0.7502810905998931	0.8002763426573032
7	0.5267692956588605	0.6555158847463464
8	0.40622313018093087	0.5777226073391916
9	0.3145315449283059	0.5032357024171782
>10	2.8758069004757183	12.099199437362945
>50	1.2212067990132545	15.646502602258018
>100	0.7681233410902879	23.58803715813436
>500	0.04933441071641747	6.041481911950095
>1k	0.030465472753166165	10.08571041541643
>5k	0.002555168682523614	3.2571024094685463
>10k+	0.0019655143711720105	5.071996378853692
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	17311	0.19997532509419438	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	17288	0.19970963088374052	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17152	0.19813856946540478	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	16319	0.1885158182780982	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14704	0.169859463935361	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	14442	0.16683286032062591	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	14363	0.1659202584673279	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	11644	0.13451058202280627	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	11513	0.13299728021543872	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10421	0.12038258117997803	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9693	0.11197278182300421	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	9308	0.10752529177845077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05181037103849932	0.0	0.0	0.0	0.0
2	0.12239261564167231	0.0	0.0	0.0	0.0
3	0.15711769375577017	0.0	0.0	0.0	0.0
4	0.18778804717989855	0.0	0.0	0.0	0.0
5	0.21703751417420852	0.0	0.0	0.0	0.0
6	0.2543040151709084	0.0	0.0	0.0	0.0
7	0.28135861694842573	0.0	0.0	0.0	0.0
8	0.3069345726851565	0.0	0.0	0.0	0.0
9	0.3320715553785288	0.0	0.0	0.0	0.0
10	0.3569197400170599	0.0	0.0	0.0	0.0
11	0.3823801765318542	0.0	0.0	0.0	0.0
12	0.4086261437558164	0.0	0.0	0.0	0.0
13	0.4360041993547558	0.0	0.0	0.0	0.0
14	0.4572943919576442	0.0	0.0	0.0	0.0
15	0.48067548247758235	0.0	0.0	0.0	1.1551922193645334E-5
16	0.4991239022208339	0.0	0.0	0.0	1.1551922193645334E-5
17	0.5225858561961276	0.0	0.0	0.0	6.9311533161872E-5
18	0.5467293735808464	0.0	0.0	0.0	6.9311533161872E-5
19	0.5670954124082431	0.0	0.0	0.0	6.9311533161872E-5
20	0.587161101258605	0.0	0.0	0.0	6.9311533161872E-5
21	0.6083935342505251	0.0	0.0	0.0	6.9311533161872E-5
22	0.6276274847029446	0.0	0.0	0.0	6.9311533161872E-5
23	0.6484440484958935	0.0	0.0	0.0	6.9311533161872E-5
24	0.6687638796345157	0.0	0.0	0.0	6.9311533161872E-5
25	0.6915904778891588	0.0	0.0	0.0	6.9311533161872E-5
26	0.7166812528937565	0.0	0.0	0.0	6.9311533161872E-5
27	0.737347641698188	0.0	0.0	0.0	6.9311533161872E-5
28	0.7587302496786256	0.0	0.0	0.0	6.9311533161872E-5
29	0.7806904537687454	0.0	0.0	0.0	6.9311533161872E-5
30	0.8041639596662327	0.0	0.0	0.0	6.9311533161872E-5
31	0.8274872905752025	0.0	0.0	0.0	6.9311533161872E-5
32	0.8487890351002846	0.0	0.0	0.0	6.9311533161872E-5
33	0.8690280027835512	0.0	0.0	0.0	6.9311533161872E-5
34	0.8886893743571356	0.0	0.0	0.0	6.9311533161872E-5
35	0.9036606655200999	0.0	0.0	0.0	6.9311533161872E-5
36	0.9182622951728676	0.0	0.0	0.0	6.9311533161872E-5
37	0.9284857463142437	0.0	0.0	0.0	6.9311533161872E-5
38	0.9369995129709603	0.0	0.0	0.0	6.9311533161872E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGTC	50	6.2937033E-10	145.69958	44
CTATCAT	5195	0.0	135.55623	44
CTATCGT	140	0.0	112.74373	44
ATATCGG	195	0.0	99.623634	44
AACGACA	1095	0.0	87.90074	43
CCGTTAT	85	2.518027E-8	85.705635	44
ACATTGG	1155	0.0	83.04665	44
ACTTCTA	2085	0.0	73.95622	44
GTGTTAG	1760	0.0	72.80373	43
TCGCAAA	1295	0.0	65.4992	43
TAACGGT	20	1.1655391E-4	64.763016	41
CCTATCA	5695	0.0	63.273197	43
CATATCG	145	0.0	62.23145	43
CGATATC	60	2.2500899E-8	60.708157	44
TTAATCG	100	1.8189894E-12	60.15707	43
TACGTTA	70	1.231092E-8	60.15707	43
ACGACAC	630	0.0	59.744537	44
TGCACCG	1425	0.0	59.523834	43
CACGAAT	105	0.0	57.700737	42
TAACGAT	175	0.0	57.700737	42
>>END_MODULE