FastQCFastQC Report
Tue 24 May 2016
ERR840935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145044
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG78090.15177712765916093No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG75590.1469180827219359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT15950.0173.1853244
CGCATTA150.004551006168.1769144
CAAACGA1000.0126.1326844
ACGACAC3900.0119.66434544
CCACTTA4650.0113.9262944
TCTATGA902.564775E-1098.10319544
CCAGAAT7650.095.6300144
ACCCATA406.729264E-494.5995244
CGAATCA453.4742698E-1094.424843
CAAATAG1300.087.32262444
CGAATAA203.6493304E-581.86787442
CGTCACA351.0750227E-981.86787442
GACAATA1101.2569217E-980.2662544
ATCACGT1101.2569217E-980.2662544
AACGACA5750.080.2317643
ACTTCTA9150.079.9529644
CCTATCA17050.077.968743
CGCTACC7750.069.983344
CCCTATC20750.069.8342842
TGTGTTA15250.068.9837642
Produced by FastQC (version 0.11.5)