##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840935.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5145044
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.251086288086164	39.0	37.0	39.0	34.0	39.0
2	37.78842824279054	39.0	38.0	39.0	35.0	39.0
3	37.917739284639744	39.0	38.0	39.0	35.0	39.0
4	38.47131297613781	39.0	38.0	40.0	35.0	41.0
5	39.0680151617751	40.0	38.0	41.0	35.0	41.0
6	39.07737076689723	40.0	38.0	41.0	35.0	41.0
7	39.03682378615227	40.0	38.0	41.0	35.0	41.0
8	38.94600920808452	40.0	38.0	41.0	35.0	41.0
9	38.79921571127477	40.0	38.0	41.0	35.0	41.0
10	38.658685717750906	40.0	38.0	41.0	35.0	41.0
11	38.53337911201537	40.0	38.0	41.0	34.0	41.0
12	38.43770276794523	40.0	38.0	41.0	34.0	41.0
13	38.269826069514664	40.0	37.0	41.0	34.0	41.0
14	38.1870469912405	40.0	37.0	41.0	34.0	41.0
15	38.19964707784812	40.0	37.0	41.0	34.0	41.0
16	38.11872532091076	40.0	37.0	41.0	34.0	41.0
17	38.13704858500724	40.0	37.0	41.0	34.0	41.0
18	38.133646281742195	40.0	37.0	41.0	34.0	41.0
19	38.20640873042097	40.0	37.0	41.0	34.0	41.0
20	38.176903054667754	40.0	37.0	41.0	34.0	41.0
21	38.135477364236344	40.0	36.0	41.0	34.0	41.0
22	37.98533481929406	40.0	36.0	41.0	33.0	41.0
23	37.79144085065162	39.0	35.0	41.0	33.0	41.0
24	37.55633984859993	39.0	35.0	41.0	33.0	41.0
25	37.43407033007602	39.0	35.0	41.0	33.0	41.0
26	37.330442385280705	39.0	35.0	41.0	33.0	41.0
27	37.20662099641871	38.0	35.0	41.0	33.0	41.0
28	37.081655082717056	38.0	35.0	40.0	33.0	41.0
29	36.926946391910946	38.0	35.0	40.0	33.0	41.0
30	36.79548152347176	38.0	35.0	40.0	32.0	41.0
31	36.60304833531526	37.0	35.0	40.0	32.0	41.0
32	36.437661882520274	37.0	35.0	40.0	32.0	41.0
33	36.35260679695562	37.0	35.0	40.0	32.0	41.0
34	36.237439043429546	36.0	35.0	40.0	32.0	41.0
35	36.04362784467556	36.0	35.0	40.0	31.0	41.0
36	35.969803083118926	36.0	35.0	40.0	31.0	41.0
37	35.877716265116135	35.0	34.0	39.0	31.0	41.0
38	35.83518188330778	35.0	34.0	39.0	31.0	41.0
39	35.725767462645265	35.0	34.0	39.0	31.0	41.0
40	35.62285138045418	35.0	34.0	39.0	31.0	41.0
41	35.505883113880266	35.0	34.0	39.0	31.0	41.0
42	35.42222078727829	35.0	34.0	39.0	31.0	41.0
43	35.28898581231958	35.0	34.0	38.0	31.0	41.0
44	35.106286726366086	35.0	34.0	38.0	31.0	41.0
45	34.955452083857274	35.0	34.0	37.0	30.0	40.0
46	34.633824909726314	35.0	33.0	37.0	30.0	40.0
47	34.50788111034643	35.0	33.0	37.0	29.0	40.0
48	34.452647216110286	35.0	33.0	37.0	29.0	40.0
49	34.528966960407516	35.0	33.0	37.0	30.0	40.0
50	34.66129036252901	35.0	33.0	37.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	20.0
22	89.0
23	230.0
24	519.0
25	1260.0
26	2821.0
27	6300.0
28	13502.0
29	27176.0
30	50227.0
31	87563.0
32	144334.0
33	242012.0
34	403040.0
35	450375.0
36	692682.0
37	874723.0
38	841039.0
39	787218.0
40	519907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.873072416873406	17.636253450893715	40.49067413223288
2	27.353216026918332	16.605086370495567	31.018957272279884	25.022740330306213
3	22.199576913239227	16.528974290598875	30.45505538922505	30.816393406936847
4	21.03921754605014	16.31228032257839	34.54178428794778	28.106717843423688
5	22.587076028893048	20.808743326587685	29.733215109530647	26.87096553498862
6	26.360999050736982	18.340601168814104	27.668840149860717	27.62955963058819
7	28.96027711327639	19.539230373928778	21.840182513502317	29.660309999292522
8	27.710744553399348	17.594251866456343	23.136692319832445	31.558311260311868
9	30.371538124844022	17.205022930804866	24.50379433101058	27.919644613340527
10	29.68744290622199	15.89504385190875	24.629779648142954	29.787733593726312
11	32.995908295439264	17.114100481939513	22.972203930617503	26.91778729200372
12	32.198325223263396	18.10089476397092	22.68316850157161	27.017611511194072
13	30.390410655380208	16.61494051362826	24.797727677353194	28.19692115363834
14	27.791560188795277	20.417687390039813	22.12725488839357	29.663497532771345
15	27.82145303324908	20.73053213927811	21.28629026301816	30.16172456445465
16	28.18450143477879	21.110528889548856	20.873757347847754	29.831212327824602
17	28.831706784237415	21.646073386350047	19.416432590275225	30.105787239137317
18	28.562710056512636	19.20290671955381	20.173743898011367	32.06063932592219
19	30.312976915260588	17.634659684154304	22.187720843592395	29.86464255699271
20	30.216748389323783	17.899691431210307	23.176808594834174	28.706751584631736
21	31.24014099782237	21.762262868888975	19.06807794063569	27.92951819265297
22	32.1974117228152	20.237455695228263	21.820843514652157	25.744289067304383
23	32.542656583694914	19.93195782193505	21.541876026716196	25.983509567653844
24	32.544988925264775	20.45292518392457	22.52519123257255	24.476894658238102
25	31.585300352969142	21.546774142663963	21.395224521332263	25.472700983034635
26	32.39243377003982	20.162409679244433	21.64277445791765	25.802382092798094
27	31.2177239261663	20.901850600636322	21.131827539500524	26.748597933696853
28	31.54348287207524	19.77344713141749	22.449590680954422	26.23347931555285
29	32.092616847488756	21.764730537685256	21.62408779251856	24.51856482230743
30	32.37127154625956	21.454991270867954	20.458994074526277	25.714743108346212
31	29.878691559561737	21.328263075468946	22.21676450239386	26.57628086257545
32	28.587259264117264	19.82821341542144	24.11867661616089	27.465850704300404
33	28.086375346881148	19.774932228144028	24.089103817478154	28.049588607496666
34	30.379122525941764	19.483957391138098	24.225086597778052	25.911833485142083
35	27.677882896692278	20.308474906509204	23.680329553863803	28.33331264293471
36	29.43653175716756	20.006090540525935	24.546488900869353	26.010888801437147
37	28.094623087120347	22.249543276485966	24.22427390826283	25.431559728130864
38	29.10940845971072	20.893535296496417	21.964095628672442	28.032960615120423
39	29.150492589026623	22.16764286683934	21.649213565808477	27.032650978325563
40	28.38700487763167	21.05888735203343	22.510834031171385	28.043273739163517
41	28.64458151384665	20.639265448412672	21.53318899906214	29.182964038678534
42	27.153059404016012	21.37064956805229	23.481281970033045	27.995009057898656
43	28.455398633093754	20.873900406497675	23.076140746172218	27.594560214236353
44	28.480161075961085	21.871255356511774	22.139303823771048	27.50927974375609
45	29.343627394172188	18.775921688070817	23.674095887585047	28.20635503017194
46	28.661857655540864	21.117218092194964	22.45268215744444	27.768242094819733
47	28.84442513001214	20.809404235518368	22.163567266051004	28.18260336841849
48	26.393564781168244	22.061767509280568	21.206727525151823	30.337940184399372
49	28.290411598455982	21.926291254828026	21.486811436956085	28.296485709759907
50	26.046385483978828	24.005379606535776	23.600546877128497	26.3476880323569
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29654.0
1	25345.0
2	30108.5
3	36281.5
4	30021.5
5	20635.0
6	12394.5
7	11427.0
8	14119.0
9	15988.0
10	16301.0
11	14845.0
12	12333.0
13	10707.0
14	10783.0
15	11409.5
16	11687.0
17	12573.5
18	13797.5
19	14119.5
20	13877.0
21	15611.0
22	18706.5
23	21486.0
24	23180.0
25	23952.0
26	25181.0
27	26406.5
28	27529.5
29	34517.5
30	43763.0
31	47438.5
32	49632.5
33	51142.5
34	55230.5
35	61334.0
36	64200.5
37	66985.0
38	71638.5
39	81868.5
40	94088.0
41	106518.5
42	125095.0
43	136509.5
44	132758.0
45	133807.0
46	151757.0
47	183877.5
48	215590.0
49	229467.5
50	245414.5
51	272688.5
52	294900.0
53	306233.5
54	317050.0
55	329931.5
56	348579.5
57	374920.5
58	389513.5
59	387668.0
60	389098.5
61	394835.5
62	390425.5
63	388464.0
64	380895.5
65	363599.5
66	344100.0
67	322357.0
68	306982.0
69	290919.5
70	267769.0
71	246484.0
72	229184.5
73	210705.5
74	187689.5
75	163386.0
76	136767.0
77	104981.0
78	84856.0
79	74926.0
80	60803.5
81	45566.0
82	35021.5
83	27228.0
84	21936.5
85	18253.0
86	14177.0
87	10959.0
88	6629.5
89	3517.5
90	2376.0
91	1309.0
92	803.0
93	759.5
94	650.0
95	402.5
96	235.0
97	218.0
98	109.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	41765.0
25	34123.0
26	25172.0
27	27848.0
28	29743.0
29	32865.0
30	34909.0
31	39309.0
32	48765.0
33	39429.0
34	38509.0
35	33814.0
36	30508.0
37	34367.0
38	35159.0
39	34362.0
40	38930.0
41	38903.0
42	52418.0
43	79564.0
44	215420.0
45	459025.0
46	577544.0
47	622476.0
48	820859.0
49	871764.0
50	807494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.458096818903385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71813020363103	17.90317486335092
2	9.395443412685314	4.220075556372296
3	2.9903785717874287	2.014746344711283
4	1.3768430177067148	1.2368509518435402
5	0.806456226606108	0.9055736008663731
6	0.5491099802922702	0.739917906097796
7	0.38920293818599544	0.6118530087587929
8	0.2973740956325471	0.534276498491966
9	0.2438437909671482	0.4928640719605788
>10	2.3802529550639697	12.42952668765254
>50	1.0179484343895993	16.679445044656585
>100	0.7818608179374043	29.72589060172495
>500	0.0381821593075734	5.684879646138014
>1k	0.014474282613328478	5.959615481865721
>5k	4.99113193563051E-4	0.8613097355086509
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	7809	0.15177712765916093	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	7559	0.1469180827219359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.017162146718278794	0.0	0.0	0.0	0.0
2	0.03350797388710378	0.0	0.0	0.0	0.0
3	0.04616092690363775	0.0	0.0	0.0	0.0
4	0.057239549360510816	0.0	0.0	0.0	0.0
5	0.06825986327813717	0.0	0.0	0.0	0.0
6	0.08369218999876386	0.0	0.0	0.0	0.0
7	0.09665612189128023	0.0	0.0	0.0	0.0
8	0.1077153081684044	0.0	0.0	0.0	0.0
9	0.11927983511899995	0.0	0.0	0.0	0.0
10	0.1288813079149566	0.0	0.0	0.0	0.0
11	0.13766646116145945	0.0	0.0	0.0	0.0
12	0.1475206042941518	0.0	0.0	0.0	0.0
13	0.15770516248257546	0.0	0.0	0.0	0.0
14	0.16963897684840012	0.0	0.0	0.0	0.0
15	0.18176715301171378	0.0	0.0	0.0	1.9436179748900106E-5
16	0.19302070108632696	0.0	0.0	0.0	1.9436179748900106E-5
17	0.20431312152043793	0.0	0.0	0.0	1.9436179748900106E-5
18	0.22141695969947	0.0	0.0	0.0	1.9436179748900106E-5
19	0.23298148665006557	0.0	0.0	0.0	1.9436179748900106E-5
20	0.24324378955748482	0.0	0.0	0.0	1.9436179748900106E-5
21	0.2567519344829704	0.0	0.0	0.0	1.9436179748900106E-5
22	0.2672863439068743	0.0	0.0	0.0	1.9436179748900106E-5
23	0.278617636700483	0.0	0.0	0.0	1.9436179748900106E-5
24	0.28967682297760716	0.0	0.0	0.0	1.9436179748900106E-5
25	0.30310722318409716	0.0	0.0	0.0	1.9436179748900106E-5
26	0.31888940114020403	0.0	0.0	0.0	1.9436179748900106E-5
27	0.33183389685297154	0.0	0.0	0.0	1.9436179748900106E-5
28	0.3458668186316774	0.0	0.0	0.0	1.9436179748900106E-5
29	0.35842259074946686	0.0	0.0	0.0	1.9436179748900106E-5
30	0.37183355477620794	0.0	0.0	0.0	1.9436179748900106E-5
31	0.386216327790394	0.0	0.0	0.0	1.9436179748900106E-5
32	0.400035451591862	0.0	0.0	0.0	1.9436179748900106E-5
33	0.41408780955031677	0.0	0.0	0.0	1.9436179748900106E-5
34	0.42536079380467884	0.0	0.0	0.0	1.9436179748900106E-5
35	0.434476362106913	0.0	0.0	0.0	1.9436179748900106E-5
36	0.4425423767027065	0.0	0.0	0.0	1.9436179748900106E-5
37	0.45000586972628415	0.0	0.0	0.0	1.9436179748900106E-5
38	0.4557395427522097	0.0	0.0	0.0	1.9436179748900106E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	1595	0.0	173.18532	44
CGCATTA	15	0.004551006	168.17691	44
CAAACGA	100	0.0	126.13268	44
ACGACAC	390	0.0	119.664345	44
CCACTTA	465	0.0	113.92629	44
TCTATGA	90	2.564775E-10	98.103195	44
CCAGAAT	765	0.0	95.63001	44
ACCCATA	40	6.729264E-4	94.59952	44
CGAATCA	45	3.4742698E-10	94.4248	43
CAAATAG	130	0.0	87.322624	44
CGAATAA	20	3.6493304E-5	81.867874	42
CGTCACA	35	1.0750227E-9	81.867874	42
GACAATA	110	1.2569217E-9	80.26625	44
ATCACGT	110	1.2569217E-9	80.26625	44
AACGACA	575	0.0	80.23176	43
ACTTCTA	915	0.0	79.95296	44
CCTATCA	1705	0.0	77.9687	43
CGCTACC	775	0.0	69.9833	44
CCCTATC	2075	0.0	69.83428	42
TGTGTTA	1525	0.0	68.98376	42
>>END_MODULE