FastQCFastQC Report
Tue 24 May 2016
ERR840934.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840934.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192192
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC110840.9297160188962851No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT108500.910088307923556No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA69010.57884971548207No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60270.5055393762078592No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC60080.5039456731801589No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA53910.45219226433326176No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA46940.39372852694867944No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGT45910.3850889789564097No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT38350.3216763742752845No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA36690.3077524425595877No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA35850.3007065975950182No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA32570.27319425059050895No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31800.2667355593729869No Hit
AATTCGTGGAGAAAGAAATGGCTCC30480.25566351728580633No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29650.24870155142795788No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC27120.22748013742752848No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC26820.22496376422589648No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA26580.22295066566459096No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24720.20734915181447283No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23610.19803857096843464No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG23580.19778693364827143No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC21760.18252093622503757No Hit
AATTCGTGGAGAAAGAAATGGCTC21010.17623000322095772No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA20870.17505569572686278No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG20750.17404914644621No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20150.1690164000429461No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC19170.16079624758428174No Hit
TAGGCTATGGTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAA18930.15878314902297616No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18560.15567962207429675No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT17870.14989196371054328No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA17850.14972420549710114No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC17680.14829826068284302No Hit
AGGAGCTGGCTGAGAGGGGACTGGGCGCCGGCGGGG16880.14158793214515783No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAA16820.14108465750483143No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA16770.1406652619712261No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG15930.13361941700665664No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC15380.12900606613699808No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC15240.12783175864290316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.12187634206570755No Hit
AAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTG14440.12112143010521795No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAA14290.11986324350440197No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATT13310.11164309104573761No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC13180.11055266265836375No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13050.1094622342709899No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAA12970.10879120141722139No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTC12800.1073652566029633No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC12790.10728137749624221No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC12720.10669422374919475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12440.10434560876100493No Hit
ATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAAAA12300.10317130126691001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT152.6549646E-5212.9563144
CATGTGG152.6549646E-5212.9563144
ACCCGAA253.7234713E-9212.9563144
AGGGACC152.6549646E-5212.9563144
GCCAATA152.6549646E-5212.9563144
ACTGGTC450.0212.9563144
AGCATAG203.1356285E-7212.956344
GAAACTC100.0022445999212.956344
ATCGTGG550.0193.5966344
AGTTGCG450.0189.294544
GTGCACC301.10740075E-8177.463644
TAATAAC301.10740075E-8177.463644
TTTACTA301.10740075E-8177.463644
CCATACT1300.0172.0031744
TAAGCCA8450.0168.8529244
CTATCAT15250.0166.1757444
ATAGAAG208.359583E-5159.7172244
CCCTGCG550.0154.877344
AGAGTAT150.0075489245141.9708744
ACCACGA302.3625835E-6141.9708744
Produced by FastQC (version 0.11.5)