##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840934.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1192192
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.27866400713979	38.0	35.0	39.0	25.0	39.0
2	36.385791047079664	39.0	35.0	39.0	31.0	39.0
3	36.69545425569036	39.0	35.0	39.0	33.0	39.0
4	37.2374550407988	39.0	35.0	39.0	33.0	41.0
5	37.92556232553146	40.0	35.0	41.0	34.0	41.0
6	37.901135890863216	40.0	35.0	41.0	34.0	41.0
7	37.936641916738246	40.0	35.0	41.0	34.0	41.0
8	37.85611881307709	39.0	35.0	41.0	34.0	41.0
9	37.78023338522654	39.0	35.0	41.0	33.0	41.0
10	37.64821018762078	39.0	35.0	41.0	33.0	41.0
11	37.49113481721065	39.0	35.0	41.0	33.0	41.0
12	37.62251969481426	39.0	35.0	41.0	33.0	41.0
13	37.48897157504832	39.0	35.0	41.0	33.0	41.0
14	37.465913208610694	39.0	35.0	41.0	33.0	41.0
15	37.41636917543483	39.0	35.0	40.0	33.0	41.0
16	37.35236270667812	38.0	35.0	40.0	33.0	41.0
17	37.44414825799871	39.0	35.0	41.0	33.0	41.0
18	37.50192670308138	39.0	35.0	41.0	33.0	41.0
19	37.542345528237064	39.0	35.0	41.0	33.0	41.0
20	37.51246443525875	39.0	35.0	41.0	33.0	41.0
21	37.56594239853983	39.0	35.0	41.0	33.0	41.0
22	37.490763232767875	39.0	35.0	41.0	33.0	41.0
23	37.44459030089113	39.0	35.0	41.0	33.0	41.0
24	37.25756925059051	38.0	35.0	41.0	33.0	41.0
25	37.22522751000706	39.0	35.0	41.0	33.0	41.0
26	37.13361075265699	38.0	35.0	41.0	33.0	41.0
27	37.09507277500427	38.0	35.0	41.0	33.0	41.0
28	37.02562171412912	38.0	35.0	41.0	33.0	41.0
29	36.945879010217475	38.0	35.0	41.0	33.0	41.0
30	36.93992460087942	38.0	35.0	41.0	33.0	41.0
31	36.86094249145323	37.0	35.0	41.0	33.0	41.0
32	36.80753031899709	37.0	35.0	40.0	33.0	41.0
33	36.707421100050155	37.0	35.0	40.0	33.0	41.0
34	36.64735553470835	37.0	35.0	40.0	33.0	41.0
35	36.50173684390138	36.0	35.0	40.0	33.0	41.0
36	36.418176941956666	36.0	35.0	40.0	33.0	41.0
37	36.27007526692234	36.0	35.0	40.0	32.0	41.0
38	36.24937318644074	36.0	35.0	40.0	33.0	41.0
39	36.18471624990925	35.0	35.0	40.0	33.0	41.0
40	36.06102811166204	35.0	35.0	39.0	32.0	41.0
41	35.91315792916428	35.0	35.0	39.0	32.0	41.0
42	35.802288465393154	35.0	35.0	39.0	32.0	41.0
43	35.70688385092273	35.0	35.0	39.0	32.0	41.0
44	35.587683402418385	35.0	34.0	39.0	32.0	41.0
45	35.3369462419114	35.0	34.0	38.0	31.0	41.0
46	35.19148131274977	35.0	34.0	38.0	31.0	41.0
47	35.037926148661924	35.0	34.0	37.0	31.0	40.0
48	35.020101680487855	35.0	34.0	38.0	30.0	40.0
49	35.10633726629521	35.0	34.0	37.0	31.0	40.0
50	35.4398436063795	36.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	10.0
21	42.0
22	82.0
23	178.0
24	388.0
25	936.0
26	1507.0
27	2833.0
28	5022.0
29	9190.0
30	15920.0
31	28568.0
32	48139.0
33	88942.0
34	175562.0
35	75697.0
36	106428.0
37	138379.0
38	152320.0
39	204393.0
40	137652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.05009931286236	24.046042919261325	34.90385776787632
2	26.118947283659004	23.986572632596094	29.07073692828001	20.82374315546489
3	25.08756978741679	23.053836965857847	24.152737143010523	27.705856103714837
4	20.828440385441272	26.442468998282152	27.898945807386728	24.83014480888984
5	20.12603674575907	24.84331382864505	27.798374758428174	27.232274667167705
6	23.60374838952115	27.695455094481424	24.5683581168134	24.132438399184025
7	27.380405169637108	25.18906350654928	20.527985425166417	26.902545898647194
8	23.74248443203779	25.646372396392525	25.010988162980457	25.60015500858922
9	24.32250845501396	29.054799898003008	25.013252898861925	21.609438748121107
10	30.108740873953188	22.106003059909813	21.942271043590296	25.842985022546706
11	21.83087958986472	26.350369739102426	23.152646553575263	28.666104117457593
12	23.129160403693362	25.80003891990552	25.075323437835518	25.995477238565602
13	23.073213039510414	25.89616437620786	23.74214891561091	27.28847366867082
14	20.73558621430105	27.744356613699807	20.749258508696585	30.770798663302557
15	23.166822122611123	28.937704665020398	22.374164564097057	25.521308648271418
16	21.382713522654072	32.9713670281297	20.893949967790423	24.75196948142581
17	23.940942398539832	34.0664087663732	19.466243692291172	22.52640514279579
18	24.823686117672324	27.28318928494739	19.43764091689929	28.455483680481
19	25.64964368155465	27.846940761219667	22.49386004938802	24.009555507837664
20	25.07817532746403	24.14569129804595	24.139819760575477	26.636313613914535
21	24.86151559480352	31.104553628945673	20.836744417006656	23.19718635924415
22	26.26951028022332	27.47040745114881	18.914235290959848	27.345846977668025
23	25.662812701309857	25.504868343354094	21.836835006441916	26.99548394889414
24	25.683279203349795	26.58607002898862	23.348420388662227	24.382230378999356
25	24.84016180367989	25.37519602000144	21.746025707679756	28.03861646863891
26	24.33872677665072	28.521594953572492	23.391242271677047	23.748435998099744
27	24.054645361756652	30.6522949147978	20.74750148157233	24.545558241873223
28	23.51634393572899	24.778859436034473	22.720625682379676	28.984170945856864
29	23.181839838183638	30.07176919511952	22.126135334959006	24.620255631737837
30	24.497135948377384	28.399580496574984	23.355347971819405	23.747935583228223
31	22.771797763828953	29.311827467354146	21.82315894188197	26.093215826934934
32	19.33481477304367	28.737316420719477	25.400640743233954	26.527228063002894
33	20.710643227097822	27.33047217919642	26.29690613668742	25.661978457018336
34	24.28339734772445	28.938670256114097	23.173269089841213	23.60466330632024
35	21.366143577898438	29.05064507496089	20.412406130502177	29.170805216638495
36	21.134914313888032	29.504051781899093	22.90381013785269	26.457223766360187
37	18.4638317276511	34.24163534329747	22.016710237791155	25.277822691260283
38	22.901293071176436	29.139571703271116	20.109347693892197	27.849787531660247
39	23.7609167345971	32.49899138240806	21.598569279392127	22.141522603602716
40	24.460560123686502	30.173381310273804	21.455925580844507	23.910132985195183
41	24.248461413387805	31.043753235564008	18.892049436614716	25.815735914433464
42	23.2845018966541	32.992704594706005	20.66190013350327	23.060893375136622
43	22.384544830268773	26.247376837414343	23.43160088551246	27.936477446804425
44	22.60292855215717	28.722081871918764	20.51261926369233	28.162370312231733
45	20.638688402190144	27.733636137381783	27.638024722083955	23.989650738344118
46	21.435236178470305	28.751434486497086	25.3617212684609	24.451608066571705
47	24.100976979348616	27.151065552029753	23.07795907653532	25.669998392086317
48	22.613268339396036	26.42362658189968	20.947743278381093	30.0153618003232
49	18.206752233560035	28.35237171314943	22.292173524377425	31.14870252891311
50	18.568603801333474	32.28622543458864	24.88443103301394	24.26073973106395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39473.0
1	27871.0
2	15429.0
3	11358.5
4	6236.5
5	4052.0
6	3345.5
7	3272.5
8	3817.0
9	3900.0
10	3546.5
11	3792.0
12	4337.5
13	3956.5
14	3506.0
15	3501.5
16	3275.5
17	3034.5
18	3630.5
19	4343.5
20	4086.5
21	3703.5
22	4157.5
23	6184.0
24	8303.0
25	9609.0
26	12052.5
27	14297.5
28	13757.0
29	12592.0
30	13816.5
31	18252.5
32	25523.0
33	30602.5
34	33115.0
35	35214.0
36	35857.0
37	37015.5
38	38054.5
39	44291.5
40	56999.0
41	65486.0
42	66373.5
43	67683.5
44	67844.5
45	60416.5
46	55754.5
47	54847.5
48	57384.0
49	68299.5
50	81959.0
51	89186.5
52	82702.5
53	65910.0
54	55705.5
55	59280.0
56	63273.5
57	62100.0
58	60499.0
59	58926.5
60	59048.0
61	56658.0
62	51723.5
63	52592.5
64	55775.5
65	55624.0
66	50226.5
67	44039.5
68	38208.0
69	32855.5
70	29530.0
71	28749.5
72	30623.5
73	30382.0
74	27151.0
75	22644.0
76	19248.0
77	16292.0
78	13846.5
79	11358.5
80	7783.0
81	5008.5
82	3587.0
83	3020.5
84	2619.5
85	1994.5
86	1342.5
87	1036.5
88	701.0
89	435.5
90	296.5
91	185.0
92	109.5
93	45.0
94	4.5
95	1.0
96	0.0
97	0.0
98	99.0
99	198.0
100	198.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	9277.0
25	10442.0
26	6482.0
27	5530.0
28	6066.0
29	6371.0
30	6072.0
31	7020.0
32	8347.0
33	6264.0
34	6807.0
35	6100.0
36	6532.0
37	5660.0
38	7099.0
39	7326.0
40	10659.0
41	12486.0
42	16447.0
43	21930.0
44	54955.0
45	97285.0
46	139384.0
47	126159.0
48	207726.0
49	170739.0
50	223027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.237826828831688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89176385118061	6.086967039034782
2	12.582553939100913	2.3247090870769647
3	5.208069310874723	1.443337272192404
4	2.909922252115504	1.0752543140162771
5	1.674530901093981	0.7734513241916839
6	1.0977640786134746	0.6084572674285952
7	0.8313042321125613	0.537561117683152
8	0.6022618038172954	0.4450872199427182
9	0.502797320024981	0.41802791151522967
>10	4.13553072867225	8.632078188075623
>50	1.7985726092577432	12.202385504460173
>100	2.60888842712729	46.090463942251816
>500	0.09672688816911254	6.077968979604072
>1k	0.0538385509620532	9.38459585810882
>5k	0.0036500712516646236	2.050688626762479
>10k+	0.0018250356258323118	1.8489663476552065
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	11084	0.9297160188962851	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	10850	0.910088307923556	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6901	0.57884971548207	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6027	0.5055393762078592	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6008	0.5039456731801589	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	5391	0.45219226433326176	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	4694	0.39372852694867944	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGT	4591	0.3850889789564097	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	3835	0.3216763742752845	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3669	0.3077524425595877	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	3585	0.3007065975950182	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3257	0.27319425059050895	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3180	0.2667355593729869	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	3048	0.25566351728580633	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2965	0.24870155142795788	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC	2712	0.22748013742752848	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	2682	0.22496376422589648	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	2658	0.22295066566459096	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2472	0.20734915181447283	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2361	0.19803857096843464	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	2358	0.19778693364827143	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	2176	0.18252093622503757	No Hit
AATTCGTGGAGAAAGAAATGGCTC	2101	0.17623000322095772	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	2087	0.17505569572686278	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	2075	0.17404914644621	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2015	0.1690164000429461	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	1917	0.16079624758428174	No Hit
TAGGCTATGGTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAA	1893	0.15878314902297616	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1856	0.15567962207429675	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT	1787	0.14989196371054328	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1785	0.14972420549710114	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1768	0.14829826068284302	No Hit
AGGAGCTGGCTGAGAGGGGACTGGGCGCCGGCGGGG	1688	0.14158793214515783	No Hit
TGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAA	1682	0.14108465750483143	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA	1677	0.1406652619712261	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	1593	0.13361941700665664	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	1538	0.12900606613699808	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	1524	0.12783175864290316	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1453	0.12187634206570755	No Hit
AAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTG	1444	0.12112143010521795	No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAA	1429	0.11986324350440197	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATT	1331	0.11164309104573761	No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	1318	0.11055266265836375	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1305	0.1094622342709899	No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAA	1297	0.10879120141722139	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTC	1280	0.1073652566029633	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	1279	0.10728137749624221	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC	1272	0.10669422374919475	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1244	0.10434560876100493	No Hit
ATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAAAA	1230	0.10317130126691001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0261702812969723	0.0	0.0	0.0	0.0
2	0.044959201202490875	0.0	0.0	0.0	0.0
3	0.06416751664161477	0.0	0.0	0.0	0.0
4	0.0782592065707537	0.0	0.0	0.0	0.0
5	0.09042167704530814	0.0	0.0	0.0	0.0
6	0.11239800300622718	0.0	0.0	0.0	0.0
7	0.12741236310929782	0.0	0.0	0.0	0.0
8	0.14133629482499463	0.0	0.0	0.0	0.0
9	0.15593125939445995	0.0	0.0	0.0	0.0
10	0.1668355432681984	0.0	0.0	0.0	0.0
11	0.1776559480352158	0.0	0.0	0.0	0.0
12	0.1937607365256603	0.0	0.0	0.0	0.0
13	0.20592320700021474	0.0	0.0	0.0	0.0
14	0.2213569626368907	0.0	0.0	0.0	0.0
15	0.23343555400472407	0.0	0.0	0.0	0.0
16	0.24853379321451577	0.0	0.0	0.0	0.0
17	0.2639675488511918	0.0	0.0	0.0	0.0
18	0.29735143332617564	0.0	0.0	0.0	0.0
19	0.30943002469400904	0.0	0.0	0.0	0.0
20	0.31949551750053684	0.0	0.0	0.0	0.0
21	0.3320773835086966	0.0	0.0	0.0	0.0
22	0.34440761219669314	0.0	0.0	0.0	0.0
23	0.35539577517715265	0.0	0.0	0.0	0.0
24	0.36571290530384365	0.0	0.0	0.0	0.0
25	0.37728822203135065	0.0	0.0	0.0	0.0
26	0.39221870302770023	0.0	0.0	0.0	0.0
27	0.40387789886192826	0.0	0.0	0.0	0.0
28	0.41645976487008807	0.0	0.0	0.0	0.0
29	0.43113860854627445	0.0	0.0	0.0	0.0
30	0.4432171999141078	0.0	0.0	0.0	0.0
31	0.45823156001717846	0.0	0.0	0.0	0.0
32	0.47181997530599096	0.0	0.0	0.0	0.0
33	0.4818015890057977	0.0	0.0	0.0	0.0
34	0.4914476862787202	0.0	0.0	0.0	0.0
35	0.5011776626583637	0.0	0.0	0.0	0.0
36	0.5110753972514495	0.0	0.0	0.0	0.0
37	0.5186245168563453	0.0	0.0	0.0	0.0
38	0.5244121752200988	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACT	15	2.6549646E-5	212.95631	44
CATGTGG	15	2.6549646E-5	212.95631	44
ACCCGAA	25	3.7234713E-9	212.95631	44
AGGGACC	15	2.6549646E-5	212.95631	44
GCCAATA	15	2.6549646E-5	212.95631	44
ACTGGTC	45	0.0	212.95631	44
AGCATAG	20	3.1356285E-7	212.9563	44
GAAACTC	10	0.0022445999	212.9563	44
ATCGTGG	55	0.0	193.59663	44
AGTTGCG	45	0.0	189.2945	44
GTGCACC	30	1.10740075E-8	177.4636	44
TAATAAC	30	1.10740075E-8	177.4636	44
TTTACTA	30	1.10740075E-8	177.4636	44
CCATACT	130	0.0	172.00317	44
TAAGCCA	845	0.0	168.85292	44
CTATCAT	1525	0.0	166.17574	44
ATAGAAG	20	8.359583E-5	159.71722	44
CCCTGCG	55	0.0	154.8773	44
AGAGTAT	15	0.0075489245	141.97087	44
ACCACGA	30	2.3625835E-6	141.97087	44
>>END_MODULE