FastQCFastQC Report
Tue 24 May 2016
ERR840933.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840933.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93382
Sequences flagged as poor quality0
Sequence length24-50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC30623.279004519072198No Hit
AATTCGTGGAGAAAGAAATGGCTCC16941.8140541003619541No Hit
AATTCGTGGAGAAAGAAATGGCTC14961.6020218029170505No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC13001.392131245850378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA11441.2250754963483328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA11441.2250754963483328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA10471.1212010880041121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.9712792615279177No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG8780.940224026043563No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT8180.8759718146966226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7890.8449165792122679No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT7150.7656721852177079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.6800025700884539No Hit
AAAAAAAAAAAAAAAAAAAAAAAA6200.6639395172517187No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC5250.5622068492857296No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCCC4660.49902550812790475No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.4776081043455912No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGCGAACATCTACAC4280.4583324409415091No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG4250.45511983037416204No Hit
AATGGTCAGCCTAAGGGTAATGTACAGCCCGTGCTTGGAGAACCATGGAA4140.4433402582938896No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4060.4347732967809642No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCGC3870.4144267631877664No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3740.40050545072926264No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.3951510997836842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3560.3812297873251804No Hit
TAAACTGAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCT3510.3758754363796021No Hit
ATCGGCATCGCGGTGTACGGCGACGACGGGACCGAAGTGGACCCGCTGCT3280.3512454220299415No Hit
TGCTGGGGGCTTCCGAGACAATCGCG3240.34696194127347885No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC3050.326615407680281No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2860.3062688740870832No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA2700.2891349510612324No Hit
AATGGTCAGCCTAAGGGTAATGTACAGCCCGTGCTTGGAGAACCATGG2610.27949711935919125No Hit
AGCGAAAAAATAGGAAAAAAAACTGAAGGAGACTATCCGACAAGAAAAAA2530.2709301578462659No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.2698592876571502No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.26771754727891883No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.2602214559551091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.2591505857659934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.24415840311837397No Hit
CCGATAAGGTGTACTTGCGTATATATATCTAATTATGTATCTCTGGTG2200.23559144160544856No Hit
ACGTCGCCGGGAACCCTAAGGACTCTGCAATATGAATAATTCCCTAGAG2170.23237883103810153No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.22702448009252318No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA2110.22595360990340752No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2070.2216701291469448No Hit
TAAACTGAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGC2050.21952838876871344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.21845751857959778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.21096142725578804No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1950.20881968687755673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.2056070763102097No Hit
ATCGGCATCGCGGTGTACGGCGACGACGGGACCGAAGTGGACCCGCTG1840.19704011479728428No Hit
TTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGT1780.1906148936625902No Hit
AGTGCAGTGCTGCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTC1730.18526054271701184No Hit
CGAGCCTGCCCTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.17883532158231777No Hit
ATGTGAATTAAGAAACCCACGCCTGTAATCATGCCTAGCATGAGCAAAGA1660.17776445139320213No Hit
AGCGAAAAAATAGGAAAAAAAACTGAAGGAGACTATCCGACAAGAAAA1650.17669358120408643No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA1640.17562271101497076No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1610.17241010044762373No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.16812661969116102No Hit
AATTCCTTCGATTCTATTTTGCTACCCGTTGTAGCGCCGGAAGATGC1570.16812661969116102No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.16598487931292968No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATCCCG1550.16598487931292968No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.16491400912381401No Hit
TAAACTGAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAT1530.16384313893469835No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1510.16170139855646698No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.15741791780000428No Hit
CCGATAAGGTGTACTTGCGTATATATATCTAATTATGTATCTCTGG1440.15420530723265724No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.15420530723265724No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.1520635668544259No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.14992182647619456No Hit
CAGTGGTATCAATGCTCAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1370.14670921590884753No Hit
CTAGGCTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAGCCCCC1360.14563834571973186No Hit
TATCAATACAGAGTGCAGTGCTGCTCAAGGGCTAGGCAGGTGCTGGGGG1320.14135486496326916No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.1349296438285751No Hit
AGTGCAGTGCTGCTCAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1230.13171703326122808No Hit
AGTGCAGTGCTGCTCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1230.13171703326122808No Hit
ATCGGCATCGCGGTGTACGGCGACGACGGGACCGAAGTGGACCCGC1220.1306461630721124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.1295752928829967No Hit
AATGGTCAGCCTAAGGGTAATGTACAGCCCGTGCTTGGAGAACCAT1190.12743355250476537No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.12743355250476537No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1263626823156497No Hit
ACGTCGCCGGGAACCCTAAGGACTCTGCAATATGAATAATTCCCTAG1180.1263626823156497No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAA1170.125291812126534No Hit
AGTGCAGTGCTGCTCAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCC1140.12207920155918699No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA1130.12100833137007132No Hit
AGTGCAGTGCTGCTCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.11779572080272428No Hit
TGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.11672485061360863No Hit
CGAGCCTGCCCTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.11565398042449294No Hit
AATTCCTTCGATTCTATTTTGCTACCCGTTGTAGCGCCGGAAGAT1080.11565398042449294No Hit
AGTGCAGTGCTGCTCAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.11565398042449294No Hit
AATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC1050.11244136985714592No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA1030.11029962947891457No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1010.10815788910068322No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1000.10708701891156754No Hit
TATCAACGCAGAGTGACTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.10708701891156754No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA990.10601614872245185No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC980.10494527853333618No Hit
AGTGCAGTGCTGCTCAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA980.10494527853333618No Hit
TCTCTCATTCATTTATGTAACAAACAGTTTTCATATACCTACTGACATT970.1038744083442205No Hit
TGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.10173266796598915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGCA108.7512453E-4290.4553544
TATACCA108.7512453E-4290.4553544
TAAGCCA255.456968E-10290.4553544
ATTCTAC108.7512453E-4290.4553544
CTATCAT700.0290.4553544
CATGGAA700.0269.7085644
AGAAAAC150.0029460334193.636944
GCCGCGG150.0029460334193.636944
CCTATCA700.0191.3588343
CCGCTGC351.8189894E-12191.3588343
TGACATT154.001546E-5191.3588343
TTAAGCC256.7757355E-9191.3588343
CCCCAGC100.003066185191.3588343
CCTAGAG205.200127E-7191.3588343
CTTTCTG154.001546E-5191.3588343
CCATGGA700.0177.6903443
GCATGCC600.0175.4122643
TTTCTGG255.757112E-5174.2732144
CATGCCT950.0168.1583744
CGCTGCT655.456968E-12156.3990544
Produced by FastQC (version 0.11.5)