FastQCFastQC Report
Tue 24 May 2016
ERR840932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607146
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA54560.33948378056505135No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36470.22692400068195423No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA35380.22014179172271842No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC26270.16345745812763746No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT25530.1588530226874223No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG25350.15773302487764024No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT24760.1540619209455768No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG24050.149644151806992No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT21820.13576862338580314No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA21790.1355819570841728No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC21240.13215974155428317No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20490.1274930840135246No Hit
AATTCGTGGAGAAAGAAATGGCTCC20400.12693308510863358No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC19230.11965309934505017No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG18020.11212422517929298No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC17470.10870200964940334No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16900.10515534991842683No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC16770.10434646261136199No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT16220.10092424708147238No Hit
CTTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCCT16090.10011535977440754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC450.0400.7574844
CTAACAG258.367351E-11400.7574844
GGTATTC152.1269134E-6400.7574844
CAAGCGT152.1269134E-6400.7574844
ACCCAAT258.367351E-11400.7574844
ATATTCA300.0400.7574844
GAACGAA103.378159E-4400.7574544
TCGTAAA201.3371391E-8400.7574544
CAAAACG600.0367.3610244
ACGATAC351.8189894E-12343.506444
ATCGAGT302.5102054E-10333.9645744
CTAACTA254.0719897E-8320.60644
TAAGCCA7850.0311.4166344
TTGCGAC206.708715E-6300.5680844
AGGGTTA500.0280.5302444
GGCTATG750.0267.1716644
AACAGCA459.094947E-12267.1716344
TTATAGG1300.0246.6199844
AGTATCG251.6346085E-5240.4544844
ACTTCTA1600.0225.4260744
Produced by FastQC (version 0.11.5)