##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840932.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1607146
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.940535582952634	39.0	37.0	39.0	33.0	39.0
2	37.52696519171251	39.0	37.0	39.0	35.0	39.0
3	37.78723650495972	39.0	37.0	39.0	35.0	40.0
4	38.46034834420768	39.0	38.0	41.0	35.0	41.0
5	38.88695550995367	40.0	38.0	41.0	35.0	41.0
6	38.877673216994594	40.0	38.0	41.0	35.0	41.0
7	38.767953253780306	40.0	38.0	41.0	35.0	41.0
8	38.73438878608415	40.0	38.0	41.0	35.0	41.0
9	38.57884037915659	40.0	38.0	41.0	35.0	41.0
10	38.44234313497343	40.0	38.0	41.0	34.0	41.0
11	38.36168711492297	40.0	37.0	41.0	34.0	41.0
12	38.23380327611804	40.0	37.0	41.0	34.0	41.0
13	38.11571568482266	40.0	37.0	41.0	34.0	41.0
14	38.09242408592623	40.0	37.0	41.0	34.0	41.0
15	38.07596260700646	40.0	37.0	41.0	34.0	41.0
16	38.04546693330911	40.0	37.0	41.0	34.0	41.0
17	38.063072676657875	40.0	36.0	41.0	34.0	41.0
18	38.12338766981967	40.0	36.0	41.0	34.0	41.0
19	38.23302985540828	40.0	36.0	41.0	34.0	41.0
20	38.20423035617175	40.0	36.0	41.0	34.0	41.0
21	38.07244581388374	40.0	36.0	41.0	34.0	41.0
22	37.97746377740417	40.0	35.0	41.0	33.0	41.0
23	37.8082545083023	39.0	35.0	41.0	33.0	41.0
24	37.593223639918214	39.0	35.0	41.0	33.0	41.0
25	37.46842107901121	39.0	35.0	41.0	33.0	41.0
26	37.31676782646007	39.0	35.0	41.0	33.0	41.0
27	37.260177895195895	39.0	35.0	41.0	33.0	41.0
28	37.172395789944936	38.0	35.0	41.0	33.0	41.0
29	36.93993332752979	38.0	35.0	41.0	33.0	41.0
30	36.84680671456648	38.0	35.0	40.0	33.0	41.0
31	36.65401858953492	37.0	35.0	40.0	32.0	41.0
32	36.4917959087531	37.0	35.0	40.0	32.0	41.0
33	36.38270992790597	37.0	35.0	40.0	32.0	41.0
34	36.31888883666648	36.0	35.0	40.0	32.0	41.0
35	36.09083007677762	36.0	35.0	40.0	32.0	41.0
36	36.027814281071265	35.0	35.0	40.0	32.0	41.0
37	35.9805402819331	35.0	35.0	39.0	32.0	41.0
38	35.91968746926747	35.0	35.0	39.0	32.0	41.0
39	35.79842944805381	35.0	35.0	39.0	32.0	41.0
40	35.670869152708185	35.0	34.0	39.0	31.0	41.0
41	35.600190571888916	35.0	34.0	39.0	31.0	41.0
42	35.481588292759284	35.0	34.0	39.0	31.0	41.0
43	35.358323083608035	35.0	34.0	38.0	31.0	41.0
44	35.18199606058784	35.0	34.0	38.0	31.0	41.0
45	34.99390158126047	35.0	34.0	38.0	30.0	40.0
46	34.71669663608128	35.0	33.0	37.0	30.0	40.0
47	34.564098284209656	35.0	33.0	37.0	30.0	40.0
48	34.66037577090141	35.0	33.0	37.0	30.0	40.0
49	34.44317953882114	35.0	33.0	37.0	29.0	40.0
50	34.86065158006026	35.0	33.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	15.0
22	51.0
23	109.0
24	233.0
25	605.0
26	1227.0
27	2508.0
28	4940.0
29	9222.0
30	16858.0
31	29654.0
32	48615.0
33	82620.0
34	141276.0
35	131255.0
36	194058.0
37	253484.0
38	264378.0
39	256055.0
40	169975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	38.91743500590488	16.84588705693198	44.236677937163144
2	24.945586773074755	18.2088621693362	30.391887233642745	26.4536638239463
3	21.129629790946186	17.111450982051412	30.961779452520183	30.79713977448222
4	19.78793463692782	17.777227457866303	35.186224524716486	27.248613380489388
5	21.87069500841865	17.654898808197885	34.61757674784992	25.856829435533545
6	26.493361524093018	19.108967075797718	27.866354394684738	26.53131700542452
7	29.968030284740777	18.094746836939517	20.627684105862194	31.30953877245751
8	29.27948052012698	19.07636269511295	23.219919036602775	28.424237748157292
9	27.230133416627982	18.330257487496468	25.330741575438697	29.10886752043685
10	28.969863347822788	16.963237938556922	25.13256418520782	28.934334528412474
11	31.139423549571728	18.248622091583467	22.817279823986123	27.794674534858686
12	28.918343448572813	18.901145259982602	21.70611755248123	30.474393738963357
13	27.252533372823628	19.670023756397985	26.172793262093176	26.90464960868521
14	29.767861787292503	20.25024484396564	21.069212131318498	28.91268123742336
15	27.371253140660524	20.11764954770755	22.11180564802451	30.399291663607414
16	27.81489671753531	23.289421122909804	19.719303660028398	29.17637849952649
17	28.01761632110586	20.563595342302442	21.576633361250316	29.842154975341384
18	27.797598973584233	20.41805784913132	19.636548266305613	32.14779491097884
19	30.45591377510195	20.454893332653036	21.074625454065778	28.014567438179235
20	30.136776621414608	19.296068932131867	20.933070175329433	29.634084271124088
21	30.706171063487698	23.205359065075605	18.223857695567172	27.864612175869524
22	31.052125942509267	22.307929708937458	20.71510615712574	25.924838191427536
23	30.936455057598998	21.487095758568294	21.208651858636365	26.367797325196342
24	32.56443409621777	20.670866243639345	22.066881291432143	24.697818368710745
25	32.936769482146794	21.863534754104936	21.784029852242426	23.415665911505844
26	31.816598019145697	22.046345110210932	21.599868438711532	24.53718843193184
27	31.577315039568482	21.719531191156	19.84835931462309	26.85479445465242
28	32.01915904461689	21.36168610784848	20.385546520523416	26.233608327011215
29	31.282996983864443	22.585363293271815	21.68692359293948	24.444716129924263
30	31.973257724651223	22.686599864677643	19.76408802397139	25.57605438669975
31	30.09918568655092	20.697295607843035	22.318789644122067	26.884729061483974
32	26.795703218540652	20.080269400341514	24.632370452874188	28.491656928243646
33	28.620007325800167	19.1941225249942	25.67081378855005	26.515056360655585
34	29.606330591215734	20.255151317361662	24.140079026474332	25.99843906494827
35	26.819777552194317	19.819143681926434	24.71347530872015	28.6476034571591
36	28.422957202354826	20.709999296366906	24.870749302230514	25.99629419904775
37	27.539621573542494	21.8771057383898	25.62363548152341	24.959637206544297
38	28.131564044044588	20.90232397255861	22.12728438058484	28.83882760281196
39	27.240173468022295	24.0030094735606	20.697777304418356	28.05903975399875
40	26.70175952051675	24.331480447138468	21.986964529973545	26.97979550237124
41	29.77959494811247	21.48382737651808	20.774969248627016	27.961608426742433
42	27.212542433995836	23.176403833279995	20.98569857293197	28.62535515979221
43	28.48237070838458	21.45315863743997	24.317679679217804	25.746790974957644
44	28.266260217080593	21.692049240478728	22.72985760017502	27.311832942265664
45	30.341217735963976	19.912380640264406	24.12693676967764	25.61946485409398
46	29.363126248232042	22.938151657083562	21.464704795343252	26.234017299341144
47	28.497434190828603	21.492707343357324	21.997106096913413	28.012752368900664
48	26.69377609615939	21.306742550999154	20.81499331690031	31.184488035941143
49	25.173463999312283	23.44660174303176	20.991702382852957	30.388231874802997
50	25.596915047698204	29.333936308690788	21.598943978292887	23.47020466531812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19509.0
1	13546.5
2	8194.0
3	7801.5
4	5849.5
5	4583.5
6	3376.0
7	2694.5
8	3172.5
9	3344.0
10	3146.0
11	2757.5
12	2376.0
13	2144.0
14	2041.5
15	2382.5
16	2730.0
17	2470.5
18	2034.0
19	2160.0
20	2528.5
21	2908.5
22	3654.0
23	4796.0
24	5383.0
25	5655.5
26	6435.0
27	7020.0
28	7423.5
29	7765.0
30	7786.5
31	8741.5
32	11925.5
33	15556.5
34	18068.5
35	18463.0
36	18448.5
37	20048.5
38	22510.0
39	26883.0
40	31941.5
41	38752.5
42	49033.0
43	58036.0
44	59618.5
45	55136.5
46	53864.5
47	58807.5
48	72360.5
49	83215.0
50	88655.5
51	93860.0
52	97304.0
53	106260.5
54	118307.0
55	122196.5
56	117729.0
57	116661.0
58	119714.5
59	121515.0
60	124785.5
61	129214.5
62	126029.0
63	115508.0
64	105430.0
65	96511.0
66	87637.0
67	82915.5
68	79048.0
69	74289.0
70	72133.5
71	68902.0
72	62408.5
73	56450.0
74	48494.0
75	41567.5
76	37918.5
77	30888.5
78	25534.5
79	24543.0
80	22390.5
81	17759.0
82	12764.0
83	9469.5
84	8076.5
85	5960.0
86	3744.0
87	2683.0
88	1813.5
89	1370.0
90	1349.0
91	1095.5
92	717.5
93	685.0
94	376.0
95	177.5
96	271.5
97	270.0
98	139.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	11018.0
25	9035.0
26	7174.0
27	7398.0
28	10257.0
29	10414.0
30	9692.0
31	9569.0
32	11936.0
33	10020.0
34	10326.0
35	8052.0
36	8215.0
37	9625.0
38	9707.0
39	9141.0
40	12542.0
41	11979.0
42	18000.0
43	32637.0
44	86631.0
45	168186.0
46	167025.0
47	233604.0
48	236389.0
49	324941.0
50	163633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.591337562375795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10214345550557	6.723733217278473
2	10.738658382093954	2.0599619501939883
3	4.328242322792855	1.2454089950900333
4	2.3274758146556116	0.8929442482651031
5	1.4843309135671503	0.7118359423146094
6	1.0299662582297873	0.5927252436323406
7	0.7697217141455297	0.5167862552622212
8	0.5447594104443734	0.417997711668225
9	0.4676858574950272	0.4037159639145589
>10	3.755599833958977	7.846503373303633
>50	1.507115751222786	10.751989854306633
>100	2.7671847747950022	53.005091545015084
>500	0.1372481820280941	8.67942209049105
>1k	0.039213766293741174	5.809872003560464
>5k	6.53562771562353E-4	0.3420116057035907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	5456	0.33948378056505135	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3647	0.22692400068195423	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	3538	0.22014179172271842	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	2627	0.16345745812763746	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	2553	0.1588530226874223	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	2535	0.15773302487764024	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	2476	0.1540619209455768	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	2405	0.149644151806992	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	2182	0.13576862338580314	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	2179	0.1355819570841728	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	2124	0.13215974155428317	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2049	0.1274930840135246	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	2040	0.12693308510863358	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	1923	0.11965309934505017	No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG	1802	0.11212422517929298	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	1747	0.10870200964940334	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1690	0.10515534991842683	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC	1677	0.10434646261136199	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT	1622	0.10092424708147238	No Hit
CTTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCCT	1609	0.10011535977440754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.012382198008146118	0.0	0.0	0.0	0.0
2	0.024639951815205337	0.0	0.0	0.0	0.0
3	0.035591041510852156	0.0	0.0	0.0	0.0
4	0.044426579788021746	0.0	0.0	0.0	0.0
5	0.053635450668452024	0.0	0.0	0.0	0.0
6	0.06968875260866157	0.0	0.0	0.0	0.0
7	0.08250650532061182	0.0	0.0	0.0	0.0
8	0.09370648341843242	0.0	0.0	0.0	0.0
9	0.10216868909234134	0.0	0.0	0.0	0.0
10	0.11199978097820609	0.0	0.0	0.0	0.0
11	0.120461986652115	0.0	0.0	0.0	0.0
12	0.12911085862765423	0.0	0.0	0.0	0.0
13	0.13968861572004038	0.0	0.0	0.0	0.0
14	0.14939526340481823	0.0	0.0	0.0	0.0
15	0.15823080168198783	0.0	0.0	0.0	0.0
16	0.16669300735589673	0.0	0.0	0.0	0.0
17	0.17751965285045665	0.0	0.0	0.0	0.0
18	0.19338628848903586	0.0	0.0	0.0	0.0
19	0.2048973770895737	0.0	0.0	0.0	0.0
20	0.21578624468467705	0.0	0.0	0.0	0.0
21	0.22642622387760664	0.0	0.0	0.0	0.0
22	0.2362573157634714	0.0	0.0	0.0	0.0
23	0.2469595170569444	0.0	0.0	0.0	0.0
24	0.26002615817106844	0.0	0.0	0.0	0.0
25	0.26979502795638977	0.0	0.0	0.0	0.0
26	0.28622166249986	0.0	0.0	0.0	0.0
27	0.29860386050800614	0.0	0.0	0.0	0.0
28	0.3108616143150653	0.0	0.0	0.0	0.0
29	0.3254838079427756	0.0	0.0	0.0	0.0
30	0.339297114263421	0.0	0.0	0.0	0.0
31	0.35031042605961127	0.0	0.0	0.0	0.0
32	0.3636259555759091	0.0	0.0	0.0	0.0
33	0.3747014894726428	0.0	0.0	0.0	0.0
34	0.38422147085579034	0.0	0.0	0.0	0.0
35	0.39280812073078614	0.0	0.0	0.0	0.0
36	0.4017681032090426	0.0	0.0	0.0	0.0
37	0.4091103110731695	0.0	0.0	0.0	0.0
38	0.41377696861392804	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATC	45	0.0	400.75748	44
CTAACAG	25	8.367351E-11	400.75748	44
GGTATTC	15	2.1269134E-6	400.75748	44
CAAGCGT	15	2.1269134E-6	400.75748	44
ACCCAAT	25	8.367351E-11	400.75748	44
ATATTCA	30	0.0	400.75748	44
GAACGAA	10	3.378159E-4	400.75745	44
TCGTAAA	20	1.3371391E-8	400.75745	44
CAAAACG	60	0.0	367.36102	44
ACGATAC	35	1.8189894E-12	343.5064	44
ATCGAGT	30	2.5102054E-10	333.96457	44
CTAACTA	25	4.0719897E-8	320.606	44
TAAGCCA	785	0.0	311.41663	44
TTGCGAC	20	6.708715E-6	300.56808	44
AGGGTTA	50	0.0	280.53024	44
GGCTATG	75	0.0	267.17166	44
AACAGCA	45	9.094947E-12	267.17163	44
TTATAGG	130	0.0	246.61998	44
AGTATCG	25	1.6346085E-5	240.45448	44
ACTTCTA	160	0.0	225.42607	44
>>END_MODULE