FastQCFastQC Report
Tue 24 May 2016
ERR840931.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840931.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366086
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA35690.9749075353878596No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT22730.6208923586261151No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT19820.5414028397698901No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG17030.46519123921701455No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG12710.34718618029643306No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT12540.3425424627000213No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC12070.32970394934523584No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA12040.32888446976939845No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA11070.3023879634839901No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCC10030.2739793381882945No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGC8990.24557071289259902No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT8830.2412001551547997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8610.23519063826532563No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG8390.22918112137585156No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC7910.2160694481624536No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCG7810.21333784957632906No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7420.20268461509044322No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA7410.2024114552318308No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300.19940669678709375No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA7220.1972214179181941No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG7200.19667509820096915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.18793398272537054No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA6500.17755390809809718No Hit
AGACGAGTCACACGCGCCCTCCCAGGTGCTGCGGGAGAACCCGCGAGG6330.1729101905016854No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC6200.16935911233972345No Hit
TGGATCACAAGGTCAGGAGCTTGAGACCAGCCTGGCCAACATGAAACCCT6180.16881279262249854No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG5810.15870587785383763No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC5700.15570111940910059No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC5370.14668684407488952No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG5280.14422840534737738No Hit
AATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC5240.14313576591292757No Hit
ATTAGGCCTGGTTTGCGCTGGAGAG5100.13931152789235318No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCT5070.1384920483165158No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC4970.13576044973039122No Hit
AGGCGTCGTGCGAACAGCAGCTGGTACCGAAGGCGGAGG4940.13494097015455384No Hit
CAAAACCAACCCGGTCAGCCCCTCTCC4920.13439465043732893No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG4860.1327556912856542No Hit
CCTTGGTCCTCCTGAACGTCGCAGCGGCGGG4820.13166305185120436No Hit
CTCTCCGCCAGACCGCCGCCGCGCCGCCATCATGGACACCAGCCGTGTG4810.1313898919925919No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.13111673213397945No Hit
TGTCAGCTGACCCAAGGGGCCTTCGAGGTGCCTTAGGCCGCTTGCCTT4770.13029725255814206No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT4720.1289314532650798No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.1270193342547926No Hit
AAGGATGATGTGGATTGTTTTTGTTTTACACCTTCTGTTGAATGGTTGC4650.1270193342547926No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCGTG4590.12538037510311784No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC4590.12538037510311784No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGG4580.1251072152445054No Hit
CTACCGGAAGTCCGCTCTAGCTCTGGGCCCTACAACTGCACCCTGAGCC4550.12428773566866803No Hit
CCATGCAAGCCATGATCAGAGCGATGGACACGCTAAGGATACAGTATGT4530.1237414159514431No Hit
TCCCTGCCACACTCAGTCCCCCACCACACTGAATCTCCCCTCCTCACAG4530.1237414159514431No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAG4450.12155613708254344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.121282977223931No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC4400.12019033778948116No Hit
CCACGTGGGGCGGAACGGAAACCTCGCAGGGTCAGACCGTAGCGACGC4380.11964401807225623No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG4290.11718557934474413No Hit
TTTTCCTGGAGGACTGCAGTGACTGCGTGCTGGCAGTGGCCTGCCAACAG4270.11663925962751923No Hit
ACACCCATGTGGCTCCAGTTCCCATTCGGCTTCTTGGCGCCCGCCCCTC4130.11281502160694482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.11199554203110745No Hit
CTTTGGTGACTCTAGATAACCTCGGG4020.1098102631622078No Hit
TGAGCCACCGCGCCCAGCCGCATTTTTCATCTTTTAATCTCACCTCCAT3990.10899078358637042No Hit
CTTTTTTTTCGTCCTTTTCCCCGGTTGCTGCTTGCTGTGAGTGTCTCTA3920.10707866457608321No Hit
CTCTTGACCCCACCTACACTACGACGACGGACGCTCGAGAACGCTGCACC3910.10680550471747076No Hit
CTCCACGGTTACCCCGGCTCTCCGCCCCTCCTTCTCGCGGCGCTCGAGGG3870.10571286528302093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.10543970542440848No Hit
TGATAGACTGGAACTGGAGCTTGTTCCATATGTGGCAGTAGGGGCCTAAG3830.10462022584857109No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC3820.10434706598995865No Hit
AGTGCAGTGCTCGTGGTGGGGTAAATTTCCTGGAAAATATAGATGAGGC3810.10407390613134618No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3810.10407390613134618No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.10161546740383408No Hit
ATGGAAACGCAGAGCGCCGGGGCAGAGGAGGGCTTTACCCAGGTCACCC3710.10134230754522161No Hit
AGTCAGCTTTCTGCCGGGCTCCGAACTAGGACTGCTGGGTGCTCCCTCCT3710.10134230754522161No Hit
ACGAGGGCCAGCTGGCCTCGGAGGAGGAGGAGGCGGAGCACGGGCTGTT3690.1007959878279967No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG3690.1007959878279967No Hit
CTCACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCT3680.10052282796938425No Hit
AGCCCTGTGAGGTGGGGCCAAGGGCTGCGAAACACCTGCCATCAGCTGA3670.10024966811077178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA350.0334.3308444
TCCATTA203.2730895E-8334.3308444
CGAAAGG203.2730895E-8334.3308444
GCGTTGA400.0334.3308444
GTTGTTA105.7979196E-4334.3308444
CAACTAT105.7979196E-4334.3308444
TTACAGC355.456968E-12286.569344
GCACCCC307.348717E-10278.6090744
CCGACAC307.348717E-10278.6090744
TTATGGA259.96406E-8267.464744
TTGCGGT259.96406E-8267.464744
TGACAGA600.0250.7481544
GGTGTCA201.3752251E-5250.7481444
GTCCTAA201.3752251E-5250.7481444
CCGAAAT401.4551915E-11250.7481444
CCAACAG500.0234.031644
CACCGTG500.0234.031644
CTATCAT150.0019524307222.8872544
TTCACGT150.0019524307222.8872544
GATCCAG150.0019524307222.8872544
Produced by FastQC (version 0.11.5)