FastQCFastQC Report
Tue 24 May 2016
ERR840930.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840930.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313429
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT148921.1338260385601353No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC89720.683097449500506No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA51430.391570461745553No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA31670.24112456782970376No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28460.21668472372697722No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC25410.19346306500008756No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC20550.15646068420904366No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA19780.1505981670878289No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA19220.14633451827239996No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG18830.14336519141879767No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18110.1378833572275319No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC18000.13704585478164408No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA16090.12250376685759183No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA15860.12075262537982638No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG15440.1175548887682547No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA15270.11626056680642807No Hit
CCTTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCC15150.11534692777455044No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC14630.11138782530308072No Hit
CTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCCTCT14510.11047418627120309No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG14090.10727644965963139No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG13570.10331734718816167No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC13420.10217529839831464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT209.933501E-8268.1562544
CGACCAT209.933501E-8268.1562544
GGATAGT209.933501E-8268.1562544
AACATAC151.0582702E-5268.1562544
GAACGTA309.094947E-12268.1562544
TCAGTAT209.933501E-8268.1562544
ACGACAC1050.0255.3868744
TTATAAC650.0247.5288244
CTATCAT17850.0240.3641544
TGTTCGA302.7903297E-9223.4635344
GGTATGA302.7903297E-9223.4635344
TGGTCCA600.0223.4635344
TATAATC550.0219.4005644
GACGTGG253.022069E-7214.52544
CATCGTA203.334708E-5201.1171744
GTAATAT900.0193.668444
TATGTGT700.0191.5401644
TAGGTAT150.0037887585178.7708344
ATGCTTG451.5097612E-10178.7708344
GGTGCGT1200.0178.7708344
Produced by FastQC (version 0.11.5)