##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840930.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1313429
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.45520009075481	39.0	37.0	39.0	35.0	39.0
2	37.84870289905278	39.0	38.0	39.0	35.0	39.0
3	38.05203783379231	39.0	38.0	39.0	35.0	40.0
4	38.68483260229522	39.0	38.0	41.0	35.0	41.0
5	39.28512085540977	40.0	39.0	41.0	35.0	41.0
6	39.37280583876251	41.0	39.0	41.0	35.0	41.0
7	39.31149304606492	40.0	39.0	41.0	35.0	41.0
8	39.25635264639352	40.0	39.0	41.0	35.0	41.0
9	39.187041705337705	40.0	39.0	41.0	35.0	41.0
10	39.0848207249878	40.0	38.0	41.0	35.0	41.0
11	38.96228726486167	40.0	38.0	41.0	35.0	41.0
12	38.92854124585341	40.0	38.0	41.0	35.0	41.0
13	38.826083480721074	40.0	38.0	41.0	35.0	41.0
14	38.843185280666106	40.0	38.0	41.0	35.0	41.0
15	38.85620844369966	40.0	38.0	41.0	35.0	41.0
16	38.819043891980456	40.0	38.0	41.0	35.0	41.0
17	38.8164895095205	40.0	38.0	41.0	35.0	41.0
18	38.89404680420487	40.0	38.0	41.0	35.0	41.0
19	38.92143465691712	40.0	38.0	41.0	35.0	41.0
20	38.82390749709349	40.0	38.0	41.0	35.0	41.0
21	38.817990922996216	40.0	38.0	41.0	35.0	41.0
22	38.81559795009856	40.0	38.0	41.0	35.0	41.0
23	38.650234614889726	40.0	38.0	41.0	35.0	41.0
24	38.44385573944233	40.0	37.0	41.0	34.0	41.0
25	38.3985062481035	40.0	37.0	41.0	34.0	41.0
26	38.38828601010743	40.0	37.0	41.0	34.0	41.0
27	38.32523281932016	40.0	37.0	41.0	34.0	41.0
28	38.23703711173391	40.0	36.0	41.0	34.0	41.0
29	38.13924869709846	40.0	36.0	41.0	34.0	41.0
30	37.98255075025517	40.0	35.0	41.0	34.0	41.0
31	37.84944138557759	40.0	35.0	41.0	33.0	41.0
32	37.78025554950729	39.0	35.0	41.0	33.0	41.0
33	37.68191257927142	39.0	35.0	41.0	33.0	41.0
34	37.56995661474906	39.0	35.0	41.0	33.0	41.0
35	37.4045701209809	39.0	35.0	41.0	33.0	41.0
36	37.34399483144303	39.0	35.0	41.0	33.0	41.0
37	37.26789835145854	38.0	35.0	41.0	33.0	41.0
38	37.23182669136652	38.0	35.0	41.0	33.0	41.0
39	37.09205240821161	38.0	35.0	41.0	33.0	41.0
40	37.009214870837965	38.0	35.0	41.0	33.0	41.0
41	36.899441699608865	37.0	35.0	40.0	33.0	41.0
42	36.84079961181284	37.0	35.0	40.0	33.0	41.0
43	36.70953220838708	37.0	35.0	40.0	33.0	41.0
44	36.566654205542925	37.0	35.0	40.0	32.0	41.0
45	36.37824254644467	36.0	35.0	40.0	32.0	41.0
46	36.101332128273576	36.0	35.0	40.0	31.0	41.0
47	36.0151453279756	36.0	35.0	40.0	31.0	41.0
48	36.10379714929097	36.0	35.0	40.0	31.0	41.0
49	36.067241814426055	36.0	34.0	40.0	31.0	41.0
50	36.60656959849073	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	1.0
21	12.0
22	22.0
23	55.0
24	108.0
25	193.0
26	430.0
27	951.0
28	2009.0
29	3889.0
30	7557.0
31	13856.0
32	24017.0
33	42325.0
34	86706.0
35	69435.0
36	107935.0
37	164573.0
38	204625.0
39	267006.0
40	317722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.58499774255022	19.483656901134356	36.93134535631542
2	28.301644017301275	18.017342391556756	31.914096612759423	21.76691697838254
3	24.0003075918074	17.564786524433373	30.127399349336738	28.307506534422494
4	21.83977969117478	17.941358078738933	34.94197250098787	25.27688972909841
5	19.8170590111837	21.725574812190075	29.3407561428901	29.116610033736123
6	22.54099764814086	24.087331709593744	27.863706374687936	25.507964267577464
7	27.654939855903898	19.364579280646307	21.730143007349465	31.25033785610033
8	26.53969114432527	21.336440721196198	24.459258932153926	27.664609202324602
9	24.409465604916598	20.84338019032624	25.421244696135076	29.32590950862209
10	27.597152187137635	17.233059419275804	26.197076507371165	28.972711886215397
11	26.640952803691707	19.92562978280516	25.179663308789436	28.253754104713693
12	29.080597428562942	21.144652661087886	23.46377307033726	26.310976840011907
13	28.186753909042668	17.23031850218017	26.696456374878274	27.886471213898883
14	24.699469860951755	20.56921234417696	22.453592847424567	32.27772494744672
15	26.720896218981004	21.19322780294938	24.648610621510564	27.437265356559053
16	26.578749212938042	23.21023823899122	22.068341722316166	28.142670825754575
17	25.349143349202734	24.81976566681564	21.472801346703932	28.35828963727769
18	27.863477964929967	23.284547546917267	19.011838477755553	29.84013601039721
19	30.30616805324079	19.81485105019	19.64026985851538	30.23871103805383
20	26.335797367044584	20.993673811070106	25.3979469008222	27.27258192106311
21	27.626160226399755	27.491474605783793	19.57486853115014	25.307496636666315
22	32.14631320002832	21.113436660832065	18.978642926264	27.76160721287561
23	31.333935827517134	22.900133924254757	21.265329149881723	24.50060109834639
24	29.696009453118517	22.78600518185604	22.691367405470718	24.826617959554724
25	29.176589764573546	23.5867056540969	21.10884297444668	26.127861606882874
26	28.480152727580645	21.77645886016266	23.173254198276428	26.570134213980268
27	28.586374983302488	24.421764137678625	21.06118200637324	25.93067887264565
28	27.827400921995004	21.606455671556926	22.05069808968779	28.515445316760278
29	30.275748706053108	24.649503328404382	20.583080072478158	24.49166789306435
30	30.54580721355582	24.493005830755575	21.47016381646612	23.491023139222488
31	28.254286693562335	22.62899386514642	23.048544269367554	26.06817517192369
32	24.315678617223245	25.36281061795325	24.90466074076649	25.416850024057013
33	25.768188231996508	21.607055833197965	25.07174640790182	27.55300952690371
34	30.437352482538177	20.75684316836473	23.76457754843298	25.041226800664113
35	26.181024167979338	22.913371551963774	23.239613086725136	27.665991193331752
36	25.39377457675558	22.738088394954183	26.369113014416744	25.499024013873495
37	24.67943220788806	27.95985170361206	22.551634275941502	24.80908181255837
38	25.918992219873356	21.47391765862396	20.75253379137559	31.854556330127092
39	24.96405777075931	24.92408540597447	23.25996841689696	26.851888406369255
40	25.697256630141336	22.92832896617437	25.258555661426072	26.11585874225822
41	25.253145392499437	22.57700716623138	23.083423074581024	29.086424366688163
42	24.716121352140487	21.680890681325547	23.859994056303925	29.74299391023004
43	24.7898050012016	22.56451060931711	23.337525486223214	29.308158903258075
44	26.741370096238438	23.3444044518156	20.64839860170651	29.265826850239453
45	26.866115771881	20.32255915060093	26.844026430535866	25.9672986469822
46	24.373999679693483	25.221791859930164	25.2680016851795	25.13620677519685
47	25.45384033615476	19.829071394225544	25.20157928946472	29.51550898015498
48	23.634737530995046	21.888082262710437	21.657432053849764	32.81974815244475
49	24.773570620729622	24.810257320982924	22.711871535794067	27.704300522493387
50	18.173150557102357	24.33398755458183	31.826088720515667	25.666773167800145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15357.0
1	11646.0
2	8754.5
3	8227.0
4	5799.5
5	3323.5
6	1544.5
7	1692.5
8	2489.0
9	2659.0
10	2493.0
11	2493.5
12	2527.0
13	2452.5
14	2245.5
15	2267.5
16	2747.0
17	2760.0
18	2383.5
19	2313.5
20	2356.0
21	2319.5
22	2148.5
23	2594.5
24	3796.0
25	4874.0
26	6129.0
27	7771.0
28	8679.5
29	9020.0
30	10261.5
31	14144.0
32	20155.0
33	23749.5
34	25836.0
35	27510.5
36	27894.0
37	29020.5
38	29022.5
39	30314.5
40	34283.0
41	40841.5
42	49187.0
43	49665.0
44	44330.5
45	42542.5
46	46977.5
47	57811.0
48	69836.5
49	84650.0
50	97741.0
51	98192.5
52	96562.0
53	89707.5
54	79481.0
55	78225.0
56	81703.5
57	85617.5
58	87339.5
59	87671.5
60	90115.5
61	94831.0
62	93071.5
63	87072.0
64	80072.5
65	71800.5
66	64513.0
67	60066.5
68	55699.0
69	50836.0
70	49036.0
71	45881.5
72	39999.5
73	35705.0
74	32181.5
75	27569.5
76	23675.0
77	20922.5
78	18638.0
79	14549.5
80	10169.0
81	6985.5
82	5391.0
83	4601.0
84	3714.0
85	3378.5
86	3177.5
87	2723.5
88	1473.0
89	427.0
90	185.0
91	92.5
92	46.0
93	39.0
94	31.0
95	18.0
96	13.0
97	13.0
98	6.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	8393.0
25	5991.0
26	3942.0
27	5942.0
28	4912.0
29	5684.0
30	6489.0
31	6333.0
32	9931.0
33	7231.0
34	7476.0
35	5944.0
36	4922.0
37	6480.0
38	7919.0
39	6816.0
40	10207.0
41	7639.0
42	13581.0
43	19328.0
44	62718.0
45	115240.0
46	152597.0
47	169141.0
48	230625.0
49	230767.0
50	197181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.74201440295919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54452245758085	6.580197106227531
2	11.991145692628761	2.3363582809114285
3	5.080430475253879	1.4848088058946827
4	2.680888838400515	1.044690307057214
5	1.5825731708527753	0.7708725312092266
6	1.0644562305268173	0.622196875746714
7	0.7620552855685375	0.519676749750191
8	0.5957515457171452	0.46430561111693025
9	0.4836331528255332	0.4240405026517417
>10	3.6949784093975895	8.020717390209718
>50	1.6413403690899164	11.814015835716168
>100	2.7379187723166565	52.549702751286645
>500	0.10895239317263535	6.961589405510435
>1k	0.029001716168255448	4.191828736445154
>5k	0.0015676603334192137	1.0778333657878376
>10k+	7.838301667096068E-4	1.13716574447839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	14892	1.1338260385601353	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8972	0.683097449500506	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5143	0.391570461745553	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3167	0.24112456782970376	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2846	0.21668472372697722	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	2541	0.19346306500008756	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	2055	0.15646068420904366	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA	1978	0.1505981670878289	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	1922	0.14633451827239996	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	1883	0.14336519141879767	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1811	0.1378833572275319	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1800	0.13704585478164408	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	1609	0.12250376685759183	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	1586	0.12075262537982638	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	1544	0.1175548887682547	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	1527	0.11626056680642807	No Hit
CCTTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCC	1515	0.11534692777455044	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	1463	0.11138782530308072	No Hit
CTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCTGGGTCCTCT	1451	0.11047418627120309	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	1409	0.10727644965963139	No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG	1357	0.10331734718816167	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	1342	0.10217529839831464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01416140499410322	0.0	0.0	0.0	0.0
2	0.028246673402216642	0.0	0.0	0.0	0.0
3	0.036469424689115286	0.0	0.0	0.0	0.0
4	0.046291044281799774	0.0	0.0	0.0	0.0
5	0.05504675167062704	0.0	0.0	0.0	0.0
6	0.07035020545457729	0.0	0.0	0.0	0.0
7	0.08298887872888447	0.0	0.0	0.0	0.0
8	0.09326731783750777	0.0	0.0	0.0	0.0
9	0.10385030329009029	0.0	0.0	0.0	0.0
10	0.11222532774896854	0.0	0.0	0.0	0.0
11	0.12326513271748987	0.0	0.0	0.0	0.0
12	0.13224924986428654	0.0	0.0	0.0	0.0
13	0.1406242743231648	0.0	0.0	0.0	0.0
14	0.14839020609412462	0.0	0.0	0.0	0.0
15	0.1582118256868091	0.0	0.0	0.0	0.0
16	0.16963231358527944	0.0	0.0	0.0	0.0
17	0.18630622591704615	0.0	0.0	0.0	0.0
18	0.2217097384023042	0.0	0.0	0.0	0.0
19	0.23495750436452978	0.0	0.0	0.0	0.0
20	0.24622571909102053	0.0	0.0	0.0	0.0
21	0.2616053094609606	0.0	0.0	0.0	0.0
22	0.2711985192956757	0.0	0.0	0.0	0.0
23	0.2852837877037891	0.0	0.0	0.0	0.0
24	0.29761791463413706	0.0	0.0	0.0	0.0
25	0.31360659769199556	0.0	0.0	0.0	0.0
26	0.32731118317015995	0.0	0.0	0.0	0.0
27	0.3423862271961408	0.0	0.0	0.0	0.0
28	0.3554817199863868	0.0	0.0	0.0	0.0
29	0.3724601786621127	0.0	0.0	0.0	0.0
30	0.3889818178218998	0.0	0.0	0.0	0.0
31	0.4030670862300132	0.0	0.0	0.0	0.0
32	0.41570575950432037	0.0	0.0	0.0	0.0
33	0.42803988643466834	0.0	0.0	0.0	0.0
34	0.43938423774714885	0.0	0.0	0.0	0.0
35	0.4501956329577008	0.0	0.0	0.0	0.0
36	0.46268203306002836	0.0	0.0	0.0	0.0
37	0.46938205262713095	0.0	0.0	0.0	0.0
38	0.47509229657636615	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	20	9.933501E-8	268.15625	44
CGACCAT	20	9.933501E-8	268.15625	44
GGATAGT	20	9.933501E-8	268.15625	44
AACATAC	15	1.0582702E-5	268.15625	44
GAACGTA	30	9.094947E-12	268.15625	44
TCAGTAT	20	9.933501E-8	268.15625	44
ACGACAC	105	0.0	255.38687	44
TTATAAC	65	0.0	247.52882	44
CTATCAT	1785	0.0	240.36415	44
TGTTCGA	30	2.7903297E-9	223.46353	44
GGTATGA	30	2.7903297E-9	223.46353	44
TGGTCCA	60	0.0	223.46353	44
TATAATC	55	0.0	219.40056	44
GACGTGG	25	3.022069E-7	214.525	44
CATCGTA	20	3.334708E-5	201.11717	44
GTAATAT	90	0.0	193.6684	44
TATGTGT	70	0.0	191.54016	44
TAGGTAT	15	0.0037887585	178.77083	44
ATGCTTG	45	1.5097612E-10	178.77083	44
GGTGCGT	120	0.0	178.77083	44
>>END_MODULE