FastQCFastQC Report
Tue 24 May 2016
ERR840929.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840929.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220297
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA35321.6032901038143959No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT19350.8783596689923149No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC14210.6450382892186458No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13420.6091776102261947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13070.5932899676346024No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAA11460.520206811713278No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA11080.5029573711852636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11070.5025034385397894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10500.47662927774776775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10230.46437309631996804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9990.4534787128285905No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA9280.4212494949999319No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8730.3962831994988584No Hit
AGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACT8660.3931056709805399No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8560.38856634452579925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8050.36541577960662197No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT7640.3468045411421853No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT7310.3318247638415412No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTC6480.2941483542671938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.2877932972305569No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5400.24512362855599487No Hit
ACAGCAACATGATCTCAGCTCACTGCAACCTCTGCCTCCTGAGCTCAAGC5190.2355910430010395No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGAC5140.2333213797736692No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5140.2333213797736692No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC5110.231959581837247No Hit
CCGATCGCACGCCCCCCCGTGGCGGCGACGACCCATTCGAACG5090.23105171654629886No Hit
ATACTTTTTGCGATGCCTACTGGAGACTTTGATTCGAAGCCCAGTTGGG5070.23014385125535072No Hit
AGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA4920.22333486157323978No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG4910.2228809289277657No Hit
CTTCCCACGGGGAGGCAGGAAGAAATAAAGG4880.22151913099134352No Hit
CTTCCCACGGGGAGGCAGGAAGAAATAAAGGTCTTTGGCTTTCTCCA4730.21471014130923252No Hit
AGGATTGAGGGAAGGGAACCCGCGCCGCCGGAAGGAGCCGCTCGCTTCAC4720.2142562086637585No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA4680.21244047808186223No Hit
CCCCTCCCGGCCTCCCGCCGCAATCTTGGCGGG4660.2115326127909141No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC4580.20790115162712158No Hit
AGCCCAGCTGTGAGCCTGGCGTGGGAGCTGCCTAGGCCGGGCCCTGCCA4570.2074472189816475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.2047236231088031No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGG4480.20336182517238094No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGA4470.20290789252690686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.19700676813574403No Hit
CTTCCCACGGGGAGGCAGGAAGAAATAAAGGTCTTTGGCTTTCTC4300.19519103755384776No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCC4300.19519103755384776No Hit
AGACACCAGAAGAACGTTGCAGGCGGCGACTCACAGTTCTAGCACTGCC4290.1947371049083737No Hit
CTGCCGCCGCCGCCGCCGGCCGCGGCTGCACTCCCAAGATGGCGGCTCCT4190.19019777845363303No Hit
CTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGA4150.1883820478717368No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC4000.18157305818962582No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.18111912554415174No Hit
AGATGGCGACCGTGTGGGATGAGGCCGAGGTGGGCACCGGGCGAGCTGG3880.17612586644393705No Hit
CAGACCGGGGCACTTGGCCTGAAGACCTGGAATTGGCGACTTCGATATT3730.16931687676182608No Hit
AGTAAGACCAACTTGAACCTGGCATATAGTAGCTGCTTAATAAATACTC3700.1679550788254039No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA3460.15706069533402633No Hit
ATCATCAACGGGTACAAACGAGTCCTGGCCTTGTCTGTGGAGACGGATTA3430.15569889739760415No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.15297530152475974No Hit
CTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTT3300.1497977730064413No Hit
CTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAA3280.14888990771549315No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCC3250.14752810977907097No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAAA3250.14752810977907097No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT3240.1470741771335969No Hit
ATACTTTTTGCGATGCCTACTGGAGACTTTGATTCGAAGCCCAGTTG3210.14571237919717472No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.14525844655170064No Hit
CAAGAGGCAGAGTTCTGTGAAGAGGAACAGTGTGGTTTGGGAAAGATGGA3150.14298878332433032No Hit
TCTAATTTGAGAGCGAGAGTGTGTATGTGTGTGTGTGTAAGTGTGTGGAT3140.14253485067885627No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.1420809180333822No Hit
TCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGTGACAGAGA3120.14162698538790813No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.14162698538790813No Hit
TCCTCTCCGATCCCGGGCGCTGAGGGCCTCTGTACGGCGCCGCGTAGGGT3110.14117305274243408No Hit
ACAGCAACATGATCTCAGCTCACTGCAACCTCTGCCTCCTGAGCTCAA3080.13981125480601186No Hit
TGAGCACCGAGCCCCCTTCTCCTTGCCCTTTTCTTTCCGTTCACCCTA3070.1393573221605378No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACT3050.13844945686958968No Hit
CCGATCGCACGCCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG3030.13754159157864154No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA2980.13527192835127122No Hit
AGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC2950.13391013041484903No Hit
ATCTTCCAAGTCTGGAAAGTTGAAAGGAGATGACCTTCAGGCCATTAAGA2950.13391013041484903No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAA2920.13254833247842684No Hit
TACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.13209439983295276No Hit
CTAGGCAGCCAGAAACCTGCGGAAAATGGTAGCGATGGCGGCTGGGCCG2850.12937080396010842No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT2780.12619327544178996No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACCC2720.12346967956894557No Hit
TCCTCCTCCGCAGAACCCGCCTCCACTCACTCCTCCTCCGCCTCCAGCG2700.12256181427799744No Hit
AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT2700.12256181427799744No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.12165394898704932No Hit
AATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC2680.12165394898704932No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC2670.12120001634157525No Hit
ACGCTGTGACAGCCACACGCCCCAAGGCCTCCAAGATGAGCTACACGTT2660.12074608369610118No Hit
AGTGTGAGCGGCGAGTGGGACGTGCGTGGCGTGCGTGCGTTGACCTGGGA2650.12029215105062711No Hit
CTTTCCCTGGTGTGATTCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGT2650.12029215105062711No Hit
TGGGAAGTGCCTGCTGTTTATAATGTTGAATTTCTGTAAAATAAACTGTA2640.11983821840515305No Hit
ACTGCGGTGAGGGGGCGAGCCGCGAAGATGGCGGCAGTGGTGGAGG2640.11983821840515305No Hit
CCCGGAGCGTCGTGGAAAGGCTTTCAAGATGAATCTGGAAAAACTCAG2640.11983821840515305No Hit
CCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTCGGG2600.11802248782325679No Hit
CCCTCACTTCATCCGGCGACTAGCACCGCGTCCGGCAGCGCCAGCCCTAC2600.11802248782325679No Hit
ATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGGGCTGACCT2570.1166606898868346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.11620675724136054No Hit
TTTTGCGAACGGCGAGCAGCGGCGACGGCGCGGAGAGACGCAGCGGAGGT2550.11575282459588647No Hit
ATACTTTTTGCGATGCCTACTGGAGACTTTGATTCGAAGCCCAGT2540.1152988919504124No Hit
CAGAGACATAGTGACCGTCCGCTCGCCGCCCCGCCTCTGCCAAGCCGC2520.11439102665946428No Hit
CCCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT2500.11348316136851615No Hit
CAGCCCGTTCGCTCACACAAAGCCCAGACGCGGAGAAAATGGCGGCAGG2500.11348316136851615No Hit
AGGATTGAGGGAAGGGAACCCGCGCCGCCGGAAGGAGCCGCTCGCTTC2490.11302922872304208No Hit
AGACACCAGAAGAACGTTGCAGGCGGCGACTCACAGTTCTAGCACTG2470.11212136343209396No Hit
CAAAACCAACCCGGTCAGCCCCTCTCC2460.11166743078661989No Hit
CTCTTGGGGCGCAGAGAGGGGCCGCAGTCTCCGCGGCTGCGTCGAGCTCC2450.11121349814114583No Hit
AGCCCAGCTGTGAGCCTGGCGTGGGAGCTGCCTAGGCCGGGCCCTGC2430.11030563285019769No Hit
AGGAGTCGCGGCTGCGAGCAGGTTAGAGTTACTTGTTATTGGTAAATA2400.1089438349137755No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC2400.1089438349137755No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG2390.10848990226830144No Hit
CGGCTCCGGAAGAGCCTCGTCCTGGGCGGCGGTGGTGCGGCGG2370.10758203697735332No Hit
ATGGATCTTTTCTAAATGTTATTACTTGTAAATAAAGTCTATTTTTCTCC2350.10667417168640518No Hit
AGGCCAAGATTTCAGCTGCGGGACGGTCAGGGGAGACCTCCAGGCGCAG2340.10622023904093111No Hit
ACCCAAACTGGGATTAGTTACCCCACTATGCTTAGCCCTAAACCT2330.10576630639545705No Hit
CTGCCGCCGCCGCCGCCGGCCGCGGCTGCACTCCCAAGATGGCGGCTC2290.10395057581356079No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA2280.10349664316808672No Hit
AGATGGCGACCGTGTGGGATGAGGCCGAGGTGGGCACCGGGCGAGCT2260.1025887778771386No Hit
CAGACCGGGGCACTTGGCCTGAAGACCTGGAATTGGCGACTTCGATA2250.10213484523166454No Hit
AGTACAGCTCTTTCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCC2250.10213484523166454No Hit
CCTAGGCCCTTCCTCGCTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGA2230.1012269799407164No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG2230.1012269799407164No Hit
ACAGGGCCGCTCAGAGGCCGCCGCAATGCTCCCCTCTTTGCAGGAG2220.10077304729524234No Hit
ACCAGCCCTTGCCTTTTCTCTTGCAGCAACAGCCCAGGCTGCTCCAAGGA2210.10031911464976828No Hit
AGTAAGACCAACTTGAACCTGGCATATAGTAGCTGCTTAATAAATAC2210.10031911464976828No Hit
ATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAAAAA2210.10031911464976828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTG203.349487E-8332.237644
GCGAACC105.893421E-4332.237644
GTAGGGT105.893421E-4332.237644
CTGGTCA203.349487E-8332.237644
TCGTTGA154.450316E-6332.2375844
TAAGCCA1400.0320.3719844
GGACATT400.0290.707944
GTTAAGA355.456968E-12284.775144
CTATCAT2750.0277.8714344
GCCCTAC251.0196527E-7265.7900744
CGGATTA401.4551915E-11249.1781944
AGCCTCA201.4031662E-5249.1781944
GGTCGCT351.895387E-9237.3125844
CTCAAGC900.0221.4917444
ATTGTAA150.001984574221.4917344
CTAGTTC150.001984574221.4917344
CCCCACA150.001984574221.4917344
CGGAGGT302.5309419E-7221.4917344
ACACGTT152.446199E-5217.008143
CGTTAAG303.8198777E-11217.008143
Produced by FastQC (version 0.11.5)