FastQCFastQC Report
Tue 24 May 2016
ERR840928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098603
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA170810.2800805364769604No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159280.26117456735583544No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90890.14903413125924084No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89130.1461482244376294No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT79820.1308824332392189No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79660.13062007807361783No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT73670.12079815656142891No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67370.11046792191588796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT69500.0126.0460944
CACCGAT351.2870441E-7117.72883644
ATGTCGG403.531306E-582.41019444
TAAGCCA33200.080.9208544
ATTAGTC5650.080.2223144
AGTACGG855.4569682E-1177.5625344
CGGATTA2050.076.3801744
TCGTACC252.1785072E-673.2049942
GGTACGA802.9376679E-972.1089244
CTATGCG1200.068.67515644
TCGAATA802.424331E-761.80764444
CAACGAA850.060.28646542
TTAAGCC32000.059.6837243
CCTTAGC44100.059.04442643
CAATGCG1700.058.17189844
AATCGCA7100.058.03534344
ACCTTAG29700.057.5534242
TACGCTA8400.057.51820842
ACGAGTG604.066169E-757.35372543
ACTTCTA25050.056.25605444
Produced by FastQC (version 0.11.5)