##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840928.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6098603
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.28214002452693	39.0	35.0	39.0	31.0	39.0
2	36.98471240052845	39.0	35.0	39.0	33.0	39.0
3	37.13938864359592	39.0	35.0	39.0	33.0	39.0
4	37.40605233690404	39.0	35.0	39.0	34.0	41.0
5	38.29685601768143	40.0	35.0	41.0	34.0	41.0
6	38.337691599207226	40.0	36.0	41.0	34.0	41.0
7	38.2981627103781	40.0	36.0	41.0	34.0	41.0
8	38.22711020868222	40.0	35.0	41.0	34.0	41.0
9	38.10018146778861	40.0	35.0	41.0	34.0	41.0
10	38.03256762245386	40.0	35.0	41.0	34.0	41.0
11	37.837891890978966	40.0	35.0	41.0	33.0	41.0
12	37.828540569045074	39.0	35.0	41.0	33.0	41.0
13	37.66146246935568	39.0	35.0	41.0	33.0	41.0
14	37.64900027104568	39.0	35.0	41.0	33.0	41.0
15	37.61762292118375	39.0	35.0	41.0	33.0	41.0
16	37.54849364682371	39.0	35.0	41.0	33.0	41.0
17	37.531397436429295	39.0	35.0	41.0	33.0	41.0
18	37.54352677162294	39.0	35.0	41.0	33.0	41.0
19	37.52388407640242	39.0	35.0	41.0	33.0	41.0
20	37.49427631213247	39.0	35.0	41.0	33.0	41.0
21	37.463625686079254	39.0	35.0	41.0	33.0	41.0
22	37.33294477440161	38.0	35.0	41.0	33.0	41.0
23	37.21014025671125	38.0	35.0	41.0	33.0	41.0
24	37.001615615904164	38.0	35.0	41.0	33.0	41.0
25	36.948664827325764	37.0	35.0	41.0	33.0	41.0
26	36.858493289493694	37.0	35.0	40.0	33.0	41.0
27	36.78376715106928	37.0	35.0	40.0	33.0	41.0
28	36.69368400466031	37.0	35.0	40.0	33.0	41.0
29	36.59130298878762	36.0	35.0	40.0	33.0	41.0
30	36.48314251295756	36.0	35.0	40.0	33.0	41.0
31	36.36620395999034	36.0	35.0	40.0	33.0	41.0
32	36.28212679133403	35.0	35.0	40.0	33.0	41.0
33	36.19759888014595	35.0	35.0	40.0	33.0	41.0
34	36.09896022629465	35.0	35.0	40.0	33.0	41.0
35	35.964568530247824	35.0	35.0	39.0	33.0	41.0
36	35.92161892086488	35.0	35.0	39.0	33.0	41.0
37	35.84731924190381	35.0	35.0	39.0	33.0	41.0
38	35.743193096437906	35.0	35.0	39.0	33.0	41.0
39	35.68732444557168	35.0	35.0	39.0	33.0	41.0
40	35.61486561475285	35.0	35.0	38.0	33.0	41.0
41	35.50421772067211	35.0	35.0	37.0	33.0	41.0
42	35.42700035164339	35.0	35.0	37.0	33.0	40.0
43	35.29966867068837	35.0	35.0	37.0	32.0	40.0
44	35.135342877858825	35.0	34.0	37.0	32.0	40.0
45	34.970833804605704	35.0	34.0	37.0	31.0	39.0
46	34.71538382365337	35.0	34.0	36.0	31.0	39.0
47	34.593308250882444	35.0	34.0	36.0	31.0	39.0
48	34.34815992544002	35.0	34.0	36.0	30.0	39.0
49	34.62770314738466	35.0	34.0	36.0	31.0	39.0
50	34.30175563369725	35.0	33.0	36.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	29.0
21	88.0
22	279.0
23	673.0
24	1571.0
25	3512.0
26	6770.0
27	13248.0
28	25389.0
29	45580.0
30	79185.0
31	134402.0
32	216567.0
33	389190.0
34	854272.0
35	499449.0
36	736276.0
37	850414.0
38	857106.0
39	869378.0
40	515219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.00374823545654	16.838512033001656	36.1577397315418
2	27.830094859429284	21.2247788550919	30.481866092939647	20.463260192539177
3	27.889255949272318	18.386423907245643	25.40239133454006	28.321928808941983
4	22.19465671072539	22.016796305645737	28.692866218706154	27.09568076492272
5	22.869270224672768	24.512220257655727	27.28975799867609	25.328751518995418
6	22.14333348145469	29.710705878051087	22.63793855740405	25.508022083090175
7	29.03983092521353	23.68963843030937	22.007039972924947	25.26349067155216
8	26.465684026325377	25.62627867398485	22.026536241168674	25.881501058521106
9	24.65207195811893	27.06000046240098	24.70631388860695	23.58161369087314
10	28.07946344433307	22.98890418018684	22.135856359235056	26.795776016245032
11	26.360545193710756	23.956125689768623	24.245946161768522	25.437382954752096
12	26.140822742519887	23.70080492204526	23.613178296734514	26.545194038700338
13	24.83588454601816	23.023682636826827	25.519696887959427	26.62073592919559
14	21.247636548894885	24.545063844949407	24.105192615423565	30.102106990732143
15	23.02668332403339	27.233564801643915	24.67402780604017	25.065724068282524
16	23.61549030163137	29.85004926538094	20.067202275668706	26.46725815731898
17	24.649432009265073	31.041666427540864	19.971295065443677	24.337606497750386
18	25.285380930681995	25.823192622966275	20.526028665909227	28.365397780442503
19	26.118637333828747	25.065592890699723	22.03975238263583	26.776017392835705
20	26.94931937691304	22.87586189820849	23.556017665029188	26.61880105984928
21	26.02848554004909	26.81033672793589	20.075564846572238	27.085612885442785
22	26.024419034982273	25.178700761469468	22.458389240945838	26.338490962602418
23	28.682699956039116	24.04412945062992	22.304829483079978	24.96834111025099
24	26.36684171768518	26.02620632954793	23.991182898772063	23.615769053994825
25	26.09545096532387	26.022002768914476	22.15859462735521	25.72395163840644
26	26.42323936573905	27.134525343127997	22.88034009604417	23.561895195088784
27	25.272122639844362	27.62395291976953	21.270073269807526	25.833851170578587
28	24.74409228357039	24.761008486518023	22.292295939944136	28.202603289967453
29	26.140472128358105	28.19061669187834	21.759197783678314	23.909713396085245
30	26.67692473814923	27.281020543137892	22.01997961883166	24.02207509988122
31	25.11100414912988	27.58855909321567	22.299317388083395	25.001119369571057
32	24.438481578018795	26.005184349489042	23.24874639890416	26.307587673588007
33	23.755996274479635	25.44784889663055	22.695692847880082	28.10046198100974
34	23.151553220352913	28.96857784213762	23.903227026208526	23.976641911300938
35	23.638950354787422	26.04225540106238	22.585102751748913	27.733691492401285
36	23.402386926450376	27.16329282394781	25.527737656273864	23.90658259332795
37	22.545603632444923	29.75056388855585	22.39121350499945	25.31261897399978
38	25.667097062281407	25.73664824235364	22.330383600289544	26.265871095075404
39	24.104996757905226	28.160989266638342	22.050725455234915	25.683288520221513
40	24.399340085092042	28.79264466724658	22.45242721456017	24.355588033101206
41	25.180969684526982	27.821666256296428	20.457847559062806	26.53951650011378
42	24.288193464642475	28.04037676490975	21.596023246088148	26.07540652435962
43	24.583296145050397	25.167895952793074	22.658820792468898	27.58998710968763
44	25.07567999197569	25.840785740746647	21.911783706144803	27.171750561132857
45	25.540107339136814	26.03975663462274	25.39490029109545	23.025235735144992
46	24.954880278668547	28.985836579567213	22.669626221070327	23.38965692069391
47	26.827189240446476	27.103488113525355	22.66103163507079	23.408291010957385
48	23.378403792750877	27.323274163848648	21.062598719958483	28.235723323441995
49	24.97568086593804	28.586967550595432	21.817032609515227	24.6203189739513
50	23.8079713043262	29.63913281092974	25.105622370364294	21.447273514379766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103907.0
1	82236.0
2	61458.5
3	56211.0
4	43202.0
5	33773.5
6	29262.0
7	26835.5
8	26944.5
9	28129.0
10	28817.0
11	28207.0
12	26323.5
13	23241.5
14	20314.5
15	19235.5
16	19663.0
17	22695.0
18	27749.0
19	29628.5
20	28638.5
21	30211.5
22	34146.0
23	41642.5
24	49406.0
25	55786.5
26	64065.0
27	71291.0
28	75573.5
29	82963.5
30	93956.0
31	107691.5
32	127149.0
33	138385.0
34	143537.0
35	153041.5
36	161205.5
37	165649.5
38	170848.0
39	186786.0
40	208113.5
41	239923.5
42	265448.5
43	263691.0
44	254966.0
45	248089.5
46	248720.5
47	250704.0
48	265184.5
49	314756.5
50	366235.0
51	389455.5
52	382384.5
53	345728.0
54	331870.5
55	333301.5
56	331176.5
57	337871.5
58	343238.0
59	349488.5
60	348679.0
61	332634.5
62	315715.0
63	310994.0
64	306380.5
65	297155.0
66	278330.5
67	264042.5
68	267100.0
69	269606.5
70	241729.5
71	209590.5
72	194661.5
73	167638.5
74	134683.5
75	117306.5
76	99808.5
77	72658.0
78	58578.0
79	54291.5
80	43248.0
81	28691.0
82	19432.0
83	15680.5
84	12661.5
85	10617.0
86	8692.5
87	5823.0
88	3458.5
89	2198.0
90	1333.5
91	825.0
92	611.5
93	530.5
94	468.0
95	282.5
96	101.0
97	13.0
98	115.0
99	216.0
100	215.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	39911.0
25	27925.0
26	25019.0
27	23318.0
28	25533.0
29	29857.0
30	30864.0
31	35460.0
32	37100.0
33	31875.0
34	37873.0
35	24743.0
36	24097.0
37	25757.0
38	33230.0
39	32590.0
40	41247.0
41	43876.0
42	60656.0
43	103969.0
44	226900.0
45	414921.0
46	809452.0
47	551914.0
48	1212002.0
49	660903.0
50	1487611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.110105096671443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22098374000393	16.723632926131828
2	9.731407158236426	4.108620556977435
3	3.1094556393653874	1.9692280602132306
4	1.3653267074680704	1.152887611437734
5	0.8046654907306063	0.8493286538493902
6	0.5428393943340039	0.6875637999002576
7	0.3750220374656016	0.5541728237126693
8	0.3018331194570138	0.5097383098714998
9	0.22551425370043948	0.4284566636772334
>10	2.385901452710133	12.21591548873467
>50	1.141150652343236	17.47977965429208
>100	0.744271777143947	25.96436366385189
>500	0.03136315427732952	4.510391013052788
>1k	0.017129107336079964	7.343632837392117
>5k	0.0026538053619278817	3.8211662712939813
>10k+	4.825100658050694E-4	1.681121665611204
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	17081	0.2800805364769604	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15928	0.26117456735583544	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9089	0.14903413125924084	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8913	0.1461482244376294	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	7982	0.1308824332392189	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7966	0.13062007807361783	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7367	0.12079815656142891	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6737	0.11046792191588796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.014790272460758636	0.0	0.0	0.0	0.0
2	0.03313873685498138	0.0	0.0	0.0	0.0
3	0.04453478936077656	0.0	0.0	0.0	0.0
4	0.055307748348269264	0.0	0.0	0.0	0.0
5	0.0671465251960162	0.0	0.0	0.0	0.0
6	0.08585572794294037	0.0	0.0	0.0	0.0
7	0.09766171039498718	0.0	0.0	0.0	0.0
8	0.10956607603413437	0.0	0.0	0.0	0.0
9	0.11983072188827507	0.0	0.0	0.0	0.0
10	0.1297018349940142	0.0	0.0	0.0	0.0
11	0.13929419573630222	0.0	0.0	0.0	0.0
12	0.1491981032377415	0.0	0.0	0.0	0.0
13	0.15760986573482486	0.0	0.0	0.0	0.0
14	0.16698906290506202	0.0	0.0	0.0	0.0
15	0.17838511541085722	0.0	0.0	0.0	0.0
16	0.18851858368219737	0.0	0.0	0.0	0.0
17	0.20281694020745406	0.0	0.0	0.0	0.0
18	0.2304462185848136	0.0	0.0	0.0	0.0
19	0.2425801449938617	0.0	0.0	0.0	0.0
20	0.2530251600243531	0.0	0.0	0.0	0.0
21	0.26379811901184586	0.0	0.0	0.0	0.0
22	0.2741447508552369	0.0	0.0	0.0	0.0
23	0.2856063921524323	0.0	0.0	0.0	0.0
24	0.2973139914173787	0.0	0.0	0.0	0.0
25	0.30774260925002006	0.0	0.0	0.0	0.0
26	0.32189339099462616	0.0	0.0	0.0	0.0
27	0.3329287051477199	0.0	0.0	0.0	0.0
28	0.3438984305094134	0.0	0.0	0.0	1.6397197850065008E-5
29	0.3572129551636662	0.0	0.0	0.0	1.6397197850065008E-5
30	0.3698060031125161	0.0	0.0	0.0	1.6397197850065008E-5
31	0.38275978941406746	0.0	0.0	0.0	1.6397197850065008E-5
32	0.39497570181236585	0.0	0.0	0.0	1.6397197850065008E-5
33	0.4054699084364075	0.0	0.0	0.0	1.6397197850065008E-5
34	0.41765302643900576	0.0	0.0	0.0	1.6397197850065008E-5
35	0.4262287609145898	0.0	0.0	0.0	1.6397197850065008E-5
36	0.4365753927579808	0.0	0.0	0.0	1.6397197850065008E-5
37	0.44301949151305636	0.0	0.0	0.0	1.6397197850065008E-5
38	0.4478730620766756	0.0	0.0	0.0	1.6397197850065008E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	6950	0.0	126.04609	44
CACCGAT	35	1.2870441E-7	117.728836	44
ATGTCGG	40	3.531306E-5	82.410194	44
TAAGCCA	3320	0.0	80.92085	44
ATTAGTC	565	0.0	80.22231	44
AGTACGG	85	5.4569682E-11	77.56253	44
CGGATTA	205	0.0	76.38017	44
TCGTACC	25	2.1785072E-6	73.20499	42
GGTACGA	80	2.9376679E-9	72.10892	44
CTATGCG	120	0.0	68.675156	44
TCGAATA	80	2.424331E-7	61.807644	44
CAACGAA	85	0.0	60.286465	42
TTAAGCC	3200	0.0	59.68372	43
CCTTAGC	4410	0.0	59.044426	43
CAATGCG	170	0.0	58.171898	44
AATCGCA	710	0.0	58.035343	44
ACCTTAG	2970	0.0	57.55342	42
TACGCTA	840	0.0	57.518208	42
ACGAGTG	60	4.066169E-7	57.353725	43
ACTTCTA	2505	0.0	56.256054	44
>>END_MODULE