FastQCFastQC Report
Tue 24 May 2016
ERR840927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5555878
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC220440.39676897152889246No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA204400.3678986471625187No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGA201990.36356089892542637No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA193990.3491617346529208No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193830.3488737513674707No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG155270.27946977957399355No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC122190.21992923530718278No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114310.20574605849876473No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC104320.18776510211347333No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87950.1583008122208587No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78060.14049984538897364No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67980.12235689840561653No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAG65770.11837912927533686No Hit
AGAACTGCGTCTCGCGACCCAGGCGCGGG58630.10552787516212558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG304.404501E-8140.905144
TAAGCCA72050.0137.4044644
TCGGTAC351.10505425E-7120.77579544
CTATCAT27050.0112.5157244
TAGTCGA351.6034397E-596.62063644
TTAAGCC70550.096.4157943
GGCGAAT751.4551915E-1190.1792644
GTCCGAA1500.076.1503943
CGACGGG25750.073.21593544
CGTCTAA900.063.4586643
GGCCGAG50850.062.44781543
GTCACAT959.338692E-962.29488444
ATTAAGC99250.061.8853742
AGGCCGA100600.059.27836242
TCCGAAC2900.058.30555744
TCAGAGA60950.058.1659742
CGCGTGT903.6379788E-1157.11279343
AACGCGT602.3162374E-456.36203844
ATAAGTC1850.055.56920243
TCACGGA14850.054.280342
Produced by FastQC (version 0.11.5)