##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840927.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5555878
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.314118488562926	39.0	37.0	39.0	35.0	39.0
2	37.78430537891581	39.0	38.0	39.0	35.0	39.0
3	37.97098550400135	39.0	38.0	39.0	35.0	40.0
4	38.35240262655155	39.0	38.0	40.0	35.0	41.0
5	39.21805428412935	40.0	39.0	41.0	35.0	41.0
6	39.18345327237207	40.0	38.0	41.0	35.0	41.0
7	39.26720547139444	40.0	39.0	41.0	35.0	41.0
8	39.149047909259345	40.0	38.0	41.0	35.0	41.0
9	39.060845288539454	40.0	38.0	41.0	35.0	41.0
10	39.05024300389606	40.0	38.0	41.0	35.0	41.0
11	38.72914776026399	40.0	38.0	41.0	35.0	41.0
12	38.918604764179484	40.0	38.0	41.0	35.0	41.0
13	38.86129897020777	40.0	38.0	41.0	35.0	41.0
14	38.85757138655672	40.0	38.0	41.0	35.0	41.0
15	38.89671119488225	40.0	38.0	41.0	35.0	41.0
16	38.79415116746624	40.0	38.0	41.0	35.0	41.0
17	38.83638319631928	40.0	38.0	41.0	35.0	41.0
18	38.83406493087141	40.0	38.0	41.0	35.0	41.0
19	38.88786794814429	40.0	38.0	41.0	35.0	41.0
20	38.91907363696611	40.0	38.0	41.0	35.0	41.0
21	38.85070154528231	40.0	38.0	41.0	35.0	41.0
22	38.60816616203596	40.0	37.0	41.0	35.0	41.0
23	38.66930231369371	40.0	37.0	41.0	35.0	41.0
24	38.49110617619753	40.0	37.0	41.0	35.0	41.0
25	38.46819879249246	40.0	37.0	41.0	35.0	41.0
26	38.441011503825024	40.0	37.0	41.0	35.0	41.0
27	38.384870294232364	40.0	37.0	41.0	35.0	41.0
28	38.27964415971746	40.0	36.0	41.0	34.0	41.0
29	38.17990166597911	40.0	36.0	41.0	34.0	41.0
30	38.125170666243264	40.0	35.0	41.0	34.0	41.0
31	37.91644984723275	40.0	35.0	41.0	33.0	41.0
32	37.84906120686352	40.0	35.0	41.0	33.0	41.0
33	37.7958724317821	39.0	35.0	41.0	33.0	41.0
34	37.709799320759316	39.0	35.0	41.0	33.0	41.0
35	37.65104071129478	39.0	35.0	41.0	33.0	41.0
36	37.5507966298411	39.0	35.0	41.0	33.0	41.0
37	37.54841211075511	39.0	35.0	41.0	33.0	41.0
38	37.351349068225964	39.0	35.0	41.0	33.0	41.0
39	37.3029879231977	39.0	35.0	41.0	33.0	41.0
40	37.30714879451739	38.0	35.0	41.0	33.0	41.0
41	37.24372314072074	38.0	35.0	41.0	33.0	41.0
42	37.08510170106397	38.0	35.0	41.0	33.0	41.0
43	37.06407836972428	38.0	35.0	41.0	33.0	41.0
44	36.88530276306851	37.0	35.0	41.0	33.0	41.0
45	36.833655316302135	37.0	35.0	40.0	33.0	41.0
46	36.58455237062298	37.0	35.0	40.0	32.0	41.0
47	36.50177861898063	37.0	35.0	40.0	32.0	41.0
48	36.111255104326176	37.0	35.0	40.0	30.0	41.0
49	36.74740005093986	37.0	35.0	40.0	32.0	41.0
50	36.44328111744031	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	13.0
21	60.0
22	152.0
23	371.0
24	606.0
25	1262.0
26	2099.0
27	4102.0
28	8295.0
29	16160.0
30	30576.0
31	55704.0
32	94687.0
33	171616.0
34	377142.0
35	277519.0
36	457854.0
37	713952.0
38	781903.0
39	1090169.0
40	1471632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.24359390180994	18.07813274517547	32.67827335301459
2	29.16860305427873	23.440705501452697	27.312730769106164	20.077960675162412
3	26.396151967339815	19.116258492357105	24.836704477672118	29.650885062630966
4	24.243818888751697	21.971810756103714	27.496914079106848	26.28745627603774
5	25.30359737920811	25.096735385478226	26.835146488097834	22.764520747215833
6	21.3271421726683	26.942366265061974	27.073344663075755	24.65714689919397
7	31.642685458535986	23.37400137296031	23.73281054767581	21.250502620827888
8	26.515989011997743	23.251824464108104	23.426612319420983	26.80557420447317
9	25.810339967868266	24.75509721415769	28.950671703014358	20.483891114959686
10	32.51657073823435	20.835662698137	21.814229902096482	24.833536661532165
11	25.239341108642055	25.93804255601005	26.67506377929825	22.147552556049646
12	27.543783358813855	21.999115171355456	25.035808921650187	25.421292548180503
13	24.703926184124274	22.780575815379674	27.253028234241285	25.262469766254767
14	19.66726051219987	23.31294891644489	24.062047438766655	32.95774313258858
15	20.663196708063065	24.63551215487453	27.885187543714963	26.81610359334744
16	26.58258514675808	26.858923108102807	20.545555535956694	26.01293620918242
17	25.75468719795503	31.19884921879134	19.894191341134558	23.152272242119068
18	23.977956319415224	23.611353597037226	19.756229348448617	32.65446073509894
19	26.175898750836502	25.56022648445484	21.30161965399528	26.96225511071337
20	29.171950859972085	22.237961308725644	22.22618999193287	26.363897839369404
21	26.391166256710463	25.52451655705903	22.133171390732482	25.951145795498032
22	23.84462005825182	26.324732112548187	27.722135007284177	22.108512821915816
23	29.189265855009776	23.986883801264174	24.168115282589	22.655735061137054
24	27.582553108617574	24.508583521812394	25.749377506129544	22.159485863440487
25	22.71487903454476	31.46281332987154	20.783511976059295	25.0387956595244
26	27.586085780404552	29.379832236188385	23.707630465372418	19.326451518034645
27	23.560329464250646	28.65595429811637	23.61168379543401	24.172032442198972
28	25.70532444099291	24.66185309891757	22.32902539552985	27.303797064559664
29	27.58821722139476	27.37217617359568	20.777088751157198	24.262517853852355
30	27.774415959667063	26.286672655020837	20.100236462266892	25.838674923045208
31	21.751731129640568	30.904577436951534	22.79720787041989	24.546483562988005
32	24.93763968327708	26.085185417135975	23.68884431279333	25.288330586793617
33	22.465862093839288	25.915955105190307	21.02787289434336	30.590309906627038
34	20.5221960878735	30.29929444829961	27.318218316329602	21.860291147497293
35	21.313014543652958	27.099484773003162	20.69612883551156	30.891371847832318
36	24.131805314516697	26.588802737243995	27.748297791710634	21.531094156528674
37	23.76841798513413	28.434551036743315	24.987159407118302	22.80987157100425
38	21.934611989084278	31.931849616351084	24.07663970211995	22.056898692444687
39	24.981910472008302	29.823874131119123	20.31121379565758	24.883001601214993
40	25.176055029542976	30.593421724308563	20.50501771014045	23.72550553600801
41	27.187170482140083	27.22553947273988	20.360802586219815	25.226487458900227
42	21.799639213425635	33.950096160058045	23.86329866270431	20.386965963812013
43	25.843789391327025	25.972075322254256	22.497689847079148	25.686445439339572
44	25.203131885642332	29.81347911083529	22.3312951746749	22.65209382884748
45	23.93871137867214	27.793774414240602	22.58610398176069	25.681410225326573
46	23.544239007163252	31.18139828088488	20.933651658714343	24.340711053237527
47	30.395351376743534	26.58594805823738	21.28628629886664	21.73241426615245
48	21.764417117201578	30.799139556444473	20.80335192766441	26.63309139868954
49	23.94352106730748	28.965742177949572	20.250381155722337	26.840355599020604
50	21.460277542116106	34.494303720761124	23.93149315234517	20.113925584777604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130269.0
1	101200.0
2	69268.5
3	59510.5
4	45299.5
5	32681.0
6	23750.0
7	19972.0
8	19709.0
9	21093.0
10	22973.5
11	23468.0
12	22775.5
13	21721.0
14	22515.0
15	23819.0
16	23879.5
17	24111.5
18	26143.0
19	28936.0
20	31147.5
21	36674.5
22	44721.0
23	55149.5
24	63873.5
25	66160.0
26	70730.0
27	78467.0
28	81015.5
29	86894.5
30	97925.5
31	106746.5
32	119556.5
33	134596.5
34	148543.0
35	154279.0
36	156949.5
37	166206.5
38	171438.5
39	181094.0
40	209592.0
41	250033.5
42	261895.0
43	272783.0
44	292203.5
45	261679.0
46	237017.5
47	236827.5
48	241554.0
49	258821.5
50	280668.5
51	301168.5
52	305760.0
53	290107.0
54	275190.5
55	269010.5
56	264230.0
57	260010.0
58	253309.0
59	248705.5
60	248298.0
61	249092.5
62	241207.0
63	231693.0
64	225379.5
65	219770.0
66	224014.5
67	235641.5
68	270513.5
69	295008.0
70	269117.0
71	242372.0
72	221007.5
73	190334.5
74	151125.5
75	115842.5
76	90511.5
77	62551.5
78	45163.5
79	39168.5
80	32002.0
81	21544.0
82	14386.0
83	11363.0
84	9071.0
85	7772.5
86	6139.5
87	4610.0
88	3214.0
89	1946.5
90	1342.0
91	1184.0
92	917.0
93	712.5
94	468.0
95	150.0
96	74.0
97	74.0
98	95.0
99	116.0
100	116.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	36575.0
25	24435.0
26	21133.0
27	19153.0
28	22281.0
29	28479.0
30	27997.0
31	26670.0
32	39588.0
33	26609.0
34	33039.0
35	27732.0
36	19935.0
37	29163.0
38	31993.0
39	28206.0
40	41086.0
41	41129.0
42	61307.0
43	83749.0
44	224917.0
45	358605.0
46	721510.0
47	508683.0
48	1112731.0
49	639013.0
50	1320160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.72115095588685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52279508460384	19.338145285477186
2	8.940575392694388	4.241614770451819
3	2.783493566545628	1.9808301323030613
4	1.250256514849648	1.1863009408931795
5	0.7348410895355497	0.8715638206730569
6	0.4614477454046798	0.6567642976198831
7	0.34025023531699267	0.5649789036311285
8	0.2620318539717313	0.4972557730651469
9	0.1994545275237367	0.42581618606030824
>10	2.00977200041401	11.548110445932874
>50	0.943967095642859	16.15923146762335
>100	0.5104607488906274	20.51287002066277
>500	0.021240648586503433	3.389248701367234
>1k	0.015759190886760612	7.932157395811515
>5k	0.001598758495758323	2.5102696590228177
>10k+	0.002055546637403558	8.184842199404608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	22044	0.39676897152889246	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	20440	0.3678986471625187	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGA	20199	0.36356089892542637	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	19399	0.3491617346529208	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19383	0.3488737513674707	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	15527	0.27946977957399355	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC	12219	0.21992923530718278	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11431	0.20574605849876473	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	10432	0.18776510211347333	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8795	0.1583008122208587	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7806	0.14049984538897364	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6798	0.12235689840561653	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAG	6577	0.11837912927533686	No Hit
AGAACTGCGTCTCGCGACCCAGGCGCGGG	5863	0.10552787516212558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.029050313919780097	0.0	0.0	0.0	0.0
2	0.055004807520971484	0.0	0.0	0.0	0.0
3	0.07048390911391503	0.0	0.0	0.0	0.0
4	0.0860350065282211	0.0	0.0	0.0	0.0
5	0.10084814677356126	0.0	0.0	0.0	0.0
6	0.12669464664270885	0.0	0.0	0.0	0.0
7	0.1450895790008348	0.0	0.0	0.0	0.0
8	0.15992071820151557	0.0	0.0	0.0	0.0
9	0.17473385844685574	0.0	0.0	0.0	0.0
10	0.1875671136047264	0.0	0.0	0.0	0.0
11	0.20245224967142908	0.0	0.0	0.0	0.0
12	0.21704940245268164	0.0	0.0	0.0	0.0
13	0.2333744549466349	0.0	0.0	0.0	0.0
14	0.24881755862889718	0.0	0.0	0.0	0.0
15	0.26481862992671906	0.0	0.0	0.0	1.7998955340632028E-5
16	0.2804597221177283	0.0	0.0	0.0	1.7998955340632028E-5
17	0.29674877670100025	0.0	0.0	0.0	1.7998955340632028E-5
18	0.3161516505582016	0.0	0.0	0.0	1.7998955340632028E-5
19	0.3311087824462668	0.0	0.0	0.0	1.7998955340632028E-5
20	0.3457419331382007	0.0	0.0	0.0	1.7998955340632028E-5
21	0.3596551256165092	0.0	0.0	0.0	1.7998955340632028E-5
22	0.37259637450642363	0.0	0.0	0.0	1.7998955340632028E-5
23	0.3860595931012164	0.0	0.0	0.0	1.7998955340632028E-5
24	0.40035076364167826	0.0	0.0	0.0	1.7998955340632028E-5
25	0.4164598286715439	0.0	0.0	0.0	1.7998955340632028E-5
26	0.43717662626861137	0.0	0.0	0.0	1.7998955340632028E-5
27	0.4513238051663481	0.0	0.0	0.0	1.7998955340632028E-5
28	0.46658691929520413	0.0	0.0	0.0	1.7998955340632028E-5
29	0.48262398850370725	0.0	0.0	0.0	1.7998955340632028E-5
30	0.4993450180151544	0.0	0.0	0.0	1.7998955340632028E-5
31	0.5154360840896794	0.0	0.0	0.0	1.7998955340632028E-5
32	0.531797134494314	0.0	0.0	0.0	1.7998955340632028E-5
33	0.5464302851862478	0.0	0.0	0.0	1.7998955340632028E-5
34	0.560901445280116	0.0	0.0	0.0	1.7998955340632028E-5
35	0.5724567746088017	0.0	0.0	0.0	1.7998955340632028E-5
36	0.5844080809549814	0.0	0.0	0.0	1.7998955340632028E-5
37	0.5915896641358935	0.0	0.0	0.0	1.7998955340632028E-5
38	0.5979072974604553	0.0	0.0	0.0	1.7998955340632028E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	30	4.404501E-8	140.9051	44
TAAGCCA	7205	0.0	137.40446	44
TCGGTAC	35	1.10505425E-7	120.775795	44
CTATCAT	2705	0.0	112.51572	44
TAGTCGA	35	1.6034397E-5	96.620636	44
TTAAGCC	7055	0.0	96.41579	43
GGCGAAT	75	1.4551915E-11	90.17926	44
GTCCGAA	150	0.0	76.15039	43
CGACGGG	2575	0.0	73.215935	44
CGTCTAA	90	0.0	63.45866	43
GGCCGAG	5085	0.0	62.447815	43
GTCACAT	95	9.338692E-9	62.294884	44
ATTAAGC	9925	0.0	61.88537	42
AGGCCGA	10060	0.0	59.278362	42
TCCGAAC	290	0.0	58.305557	44
TCAGAGA	6095	0.0	58.16597	42
CGCGTGT	90	3.6379788E-11	57.112793	43
AACGCGT	60	2.3162374E-4	56.362038	44
ATAAGTC	185	0.0	55.569202	43
TCACGGA	1485	0.0	54.2803	42
>>END_MODULE