FastQCFastQC Report
Tue 24 May 2016
ERR840926.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840926.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10256358
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA618720.6032550735845998No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA512040.4992415436356648No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA420160.40965808720795427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA369170.359942583907465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA357440.34850577563692686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA334840.3264706633680299No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA324220.31611611061158357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA285450.27831516801578104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA279320.2723383875640846No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA261980.25543180142502825No Hit
AAAAAAAAAAAAAAAAAAAAAAAA210870.20559929752842093No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA199950.1949522432816795No Hit
TGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA193570.18873171158807053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192430.18762020592494918No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA185330.180697670654632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166260.16210432592154056No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA143590.14000096330490805No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133650.13030941392646397No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA127650.1244593841205621No Hit
TGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA118730.11576233980912132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118280.1153235875736787No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG115950.11305182599905346No Hit
TGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA113350.11051681308316266No Hit
TGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA105620.10298002468322576No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA103450.10086426390342458No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA103270.10068876300924753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTGG25000.0146.1767144
CTATCAT50800.0144.3433444
TTTACGC4900.0111.7484644
TCGTGCG19500.087.1129943
GTGTTAG22000.085.1106543
AGGTCGA4800.083.1120643
CCTATCA51150.078.8742943
GCGATAA900.078.6436843
TATCATA15600.078.59453644
GGTGCGT8150.071.56789444
TGTGTTA29750.071.2995242
CACGGAT2400.069.437944
ACGTGTT3100.069.1179144
CCCTATC64800.067.86796642
ACGACAC7350.066.4012544
CGTGTGA28300.064.11745543
TACGCTA8850.062.48255542
AAAGTTG31850.061.8195143
AACGACA10900.061.39322743
CGTGCGC24400.060.00605444
Produced by FastQC (version 0.11.5)