##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840926.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10256358
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.31420519837548	39.0	35.0	39.0	31.0	39.0
2	37.15258262240846	39.0	37.0	39.0	33.0	39.0
3	36.64987415610882	39.0	35.0	39.0	33.0	39.0
4	37.40417495177138	39.0	35.0	39.0	34.0	41.0
5	38.21082883417291	40.0	37.0	41.0	34.0	41.0
6	38.122923068793035	40.0	36.0	41.0	34.0	41.0
7	38.27038993763673	40.0	37.0	41.0	34.0	41.0
8	37.889800063531325	39.0	36.0	41.0	33.0	41.0
9	38.07623310340766	39.0	36.0	41.0	34.0	41.0
10	38.05221756104847	40.0	36.0	41.0	34.0	41.0
11	37.87477406697387	39.0	36.0	41.0	33.0	41.0
12	37.952485082911494	39.0	36.0	41.0	33.0	41.0
13	37.442851253827136	39.0	35.0	40.0	33.0	41.0
14	37.520739818169375	39.0	35.0	40.0	33.0	41.0
15	37.223682324661446	38.0	35.0	40.0	33.0	41.0
16	37.346372659768704	38.0	35.0	40.0	33.0	41.0
17	37.47039299915233	39.0	35.0	40.0	33.0	41.0
18	37.48569794463103	39.0	35.0	40.0	33.0	41.0
19	37.5149854363508	39.0	35.0	41.0	33.0	41.0
20	37.42698558299155	39.0	35.0	41.0	33.0	41.0
21	37.14716305729578	38.0	35.0	40.0	33.0	41.0
22	37.19256806363428	38.0	35.0	40.0	33.0	41.0
23	37.137082383434745	38.0	35.0	40.0	33.0	41.0
24	36.871317576863056	38.0	35.0	40.0	33.0	41.0
25	36.70365838766202	37.0	35.0	40.0	33.0	41.0
26	36.48177796078942	37.0	35.0	40.0	33.0	41.0
27	36.307118498444716	36.0	35.0	40.0	33.0	41.0
28	36.13409645865622	35.0	35.0	40.0	33.0	41.0
29	35.9793998918737	35.0	35.0	39.0	33.0	41.0
30	35.834317151154934	35.0	35.0	39.0	33.0	41.0
31	35.690040248950474	35.0	35.0	39.0	32.0	41.0
32	35.57393546144306	35.0	34.0	39.0	32.0	41.0
33	35.462940487642115	35.0	34.0	39.0	32.0	41.0
34	35.399372800391106	35.0	34.0	38.0	32.0	41.0
35	35.282302611626854	35.0	34.0	38.0	32.0	41.0
36	35.21528339223287	35.0	34.0	37.0	32.0	40.0
37	35.180814218310886	35.0	34.0	37.0	32.0	40.0
38	35.1405063109328	35.0	34.0	37.0	32.0	40.0
39	35.05920779108825	35.0	34.0	37.0	32.0	40.0
40	34.96613435114367	35.0	34.0	36.0	31.0	40.0
41	34.88422238643777	35.0	34.0	36.0	31.0	40.0
42	34.793896239276954	35.0	34.0	36.0	31.0	40.0
43	34.695265627398484	35.0	34.0	36.0	31.0	40.0
44	34.541700528642494	35.0	34.0	36.0	31.0	40.0
45	34.29809160242579	35.0	33.0	36.0	30.0	39.0
46	34.098994903926354	35.0	33.0	36.0	29.0	39.0
47	33.85403083593815	35.0	33.0	35.0	28.0	39.0
48	33.88523316748599	35.0	33.0	36.0	28.0	39.0
49	33.94089010930744	35.0	33.0	35.0	29.0	39.0
50	34.24288482323393	35.0	33.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	24.0
21	121.0
22	373.0
23	949.0
24	2326.0
25	5382.0
26	11242.0
27	23310.0
28	44666.0
29	81859.0
30	140619.0
31	238552.0
32	395948.0
33	691959.0
34	1230359.0
35	1006792.0
36	1814554.0
37	1845377.0
38	1207605.0
39	990402.0
40	523933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.41396760916497	18.184291148963403	31.401741241871626
2	37.59603555180114	20.778067614254496	23.995428006705694	17.630468827238673
3	22.16496343048868	17.71388050222116	34.635062465643266	25.486093601646903
4	29.081248918963244	22.828366560527627	28.079567815398022	20.01081670511111
5	22.723144024418804	20.320137031098174	24.12397266164071	32.832746282842315
6	19.70642990426036	33.95156448322104	26.5855482033681	19.7564574091505
7	39.47350511750857	19.895717368679993	18.453295019538125	22.17748249427331
8	21.571204905288994	21.395509010118406	31.200295465505395	25.832990619087205
9	34.24040970488745	21.961665144683913	23.540071436663972	20.25785371376467
10	24.045426261446803	18.546359243700348	24.07629491872261	33.33191957613024
11	23.244820432360104	23.13817438899851	32.04465951754024	21.572345661101146
12	28.978054393187136	19.933362310480973	30.254355395940742	20.834227900391152
13	23.66568132664636	30.798339917541877	23.751569514246675	21.784409241565086
14	32.63289951462303	20.692910680379917	19.416229425688925	27.257960379308134
15	24.03968348218734	23.000532937715317	31.137729396731277	21.822054183366063
16	25.303845673093704	24.398914312468424	17.1092799217812	33.187960092656674
17	37.177885171324945	24.389661515325418	16.848007840599948	21.58444547274968
18	33.24207286836127	24.061835595052354	18.36565182299604	24.33043971359034
19	32.791425572313294	25.347399144998644	18.139080168613457	23.722095114074605
20	30.008459143099337	29.708762116142985	18.59787850619099	21.684900234566694
21	25.163513208099797	37.639940025494425	16.261210850869283	20.93533591553649
22	24.51758216708114	36.76508756812116	18.041891673438077	20.675438591359622
23	25.01349894377712	37.19510375905365	17.674207550087466	20.117189747081763
24	24.45579610228114	37.567009653914184	19.112671379060675	18.864522864743996
25	25.007141540661813	38.29294594874642	17.150630074294657	19.549282436297112
26	25.198849129145888	38.68082058111421	17.12130151429906	18.999028775440845
27	23.714491641273362	38.76761593505923	17.539597716446476	19.97829470722093
28	23.211827185174748	37.87089930995782	17.956809909158583	20.96046359570885
29	23.910078587600385	37.85543926337248	18.277836541090593	19.95664560793654
30	24.30250822925845	38.05445293661732	17.80003993992361	19.842998894200623
31	23.422039217239895	37.65941707107654	18.013181378308502	20.90536233337506
32	22.825269961709168	36.291734867602074	21.0280700774612	19.854925093227553
33	21.41325615307371	35.85967750280609	20.09710183338314	22.62996451073706
34	22.96180621393073	36.63904179162413	20.30516148271299	20.09399051173215
35	21.99737522242172	36.12099741547974	18.92025560717439	22.961371754924144
36	22.28734990129608	36.55520069797464	20.820874647316142	20.336574753413135
37	21.754665339745	38.1142474769106	20.097021356034023	20.034065827310386
38	22.581593033178407	36.035574848631136	19.964517689130354	21.418314429060104
39	23.55366482079893	37.55054040903302	18.678048700055918	20.217746070112135
40	22.73443945202345	37.924324749487624	18.37353314736143	20.967702651127496
41	21.804486204480913	37.84110664850272	18.13048172544344	22.223925421572925
42	20.479679441957597	38.00926899631338	19.384363053072367	22.12668850865666
43	21.915624684457846	36.69657933335318	19.90492149457107	21.482874487617906
44	22.16034809591582	36.843588246367304	18.85437212163214	22.141691536084736
45	22.620372339221152	35.27892426734541	20.105159259976528	21.99554413345691
46	22.75005281928594	34.27755497047793	20.16470137749538	22.80769083274075
47	22.655752952892037	33.47220685458217	21.21352106442422	22.658519128101574
48	22.042681724976042	32.796585944529575	20.06681715497819	25.0939151755162
49	23.222247751750157	32.85055746312903	19.645421636221368	24.281773148899454
50	22.39470655447725	32.758541806918295	22.23825852487274	22.60849311373172
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432598.0
1	361578.5
2	191928.5
3	84312.5
4	69766.0
5	51627.5
6	30508.5
7	21519.0
8	21102.5
9	20596.0
10	19624.5
11	19850.0
12	20877.5
13	21922.0
14	24932.0
15	31366.5
16	39855.5
17	53536.5
18	73125.5
19	97597.0
20	126709.0
21	181821.5
22	271055.5
23	385959.5
24	490426.5
25	539220.0
26	518063.5
27	423612.0
28	304424.0
29	233000.0
30	202463.0
31	188816.5
32	194427.0
33	198806.5
34	204618.5
35	211550.0
36	211746.0
37	207793.0
38	206999.0
39	227124.0
40	256585.0
41	293756.5
42	335993.0
43	346705.5
44	337467.5
45	343719.5
46	365904.5
47	373847.0
48	385070.0
49	429449.5
50	484598.5
51	531132.0
52	552277.5
53	550297.0
54	557815.0
55	557490.0
56	547166.0
57	553030.0
58	563887.0
59	566763.5
60	567694.5
61	560868.0
62	534433.0
63	501483.0
64	456176.5
65	421230.0
66	405970.5
67	391698.5
68	389073.5
69	374983.5
70	323768.5
71	273891.0
72	252248.5
73	236246.0
74	208872.5
75	177643.5
76	151100.5
77	121438.5
78	98947.5
79	85498.5
80	68305.0
81	51185.0
82	39617.5
83	31384.5
84	25110.5
85	20182.0
86	14906.5
87	11128.5
88	7195.5
89	4500.0
90	3082.5
91	1635.0
92	964.5
93	657.0
94	391.5
95	209.5
96	169.0
97	165.0
98	84.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	93985.0
25	79226.0
26	78214.0
27	89742.0
28	95194.0
29	90384.0
30	87374.0
31	80420.0
32	91349.0
33	77607.0
34	77788.0
35	63163.0
36	59709.0
37	71602.0
38	84020.0
39	86125.0
40	104359.0
41	121312.0
42	158180.0
43	226375.0
44	434697.0
45	943114.0
46	1178259.0
47	1141939.0
48	1530356.0
49	1428878.0
50	1682987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.472744739824142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55336435488869	10.500463223237597
2	11.883594086915773	3.4397644762323107
3	4.200931456572604	1.8239702592141878
4	2.063265848389672	1.194444798165617
5	1.284335627940116	0.9293930851719524
6	0.8630082531376877	0.7494058893613975
7	0.6342622867239798	0.6425661321697318
8	0.502221052858369	0.5814813680787922
9	0.3883515806355628	0.5058461966261159
>10	3.4728196629909793	11.631634654194595
>50	1.2735811815842961	13.215736472557122
>100	0.7770067524062315	19.497896211178293
>500	0.05350945132665356	5.4020479408999975
>1k	0.04188439480840294	11.509568199161842
>5k	0.00341913427007371	3.2947360921984954
>10k+	0.004444874551095823	15.08104500155191
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	61872	0.6032550735845998	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	51204	0.4992415436356648	No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	42016	0.40965808720795427	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	36917	0.359942583907465	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	35744	0.34850577563692686	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	33484	0.3264706633680299	No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	32422	0.31611611061158357	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28545	0.27831516801578104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	27932	0.2723383875640846	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	26198	0.25543180142502825	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	21087	0.20559929752842093	No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA	19995	0.1949522432816795	No Hit
TGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	19357	0.18873171158807053	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19243	0.18762020592494918	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA	18533	0.180697670654632	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16626	0.16210432592154056	No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	14359	0.14000096330490805	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13365	0.13030941392646397	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	12765	0.1244593841205621	No Hit
TGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11873	0.11576233980912132	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11828	0.1153235875736787	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	11595	0.11305182599905346	No Hit
TGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	11335	0.11051681308316266	No Hit
TGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10562	0.10298002468322576	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	10345	0.10086426390342458	No Hit
AGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA	10327	0.10068876300924753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.08316792374057146	0.0	0.0	0.0	0.0
2	0.13192792217276347	0.0	0.0	0.0	0.0
3	0.1556107928369895	0.0	0.0	0.0	0.0
4	0.20742255681792698	0.0	0.0	0.0	0.0
5	0.2389054672233555	0.0	0.0	0.0	0.0
6	0.3738266546468054	0.0	0.0	0.0	0.0
7	0.39848453027868175	0.0	0.0	0.0	0.0
8	0.4296164388957562	0.0	0.0	0.0	0.0
9	0.45946134095553215	0.0	0.0	0.0	0.0
10	0.48426546733255604	0.0	0.0	0.0	0.0
11	0.5123553604505615	0.0	0.0	0.0	0.0
12	0.537520238665616	0.0	0.0	0.0	0.0
13	0.5779536946740743	0.0	0.0	0.0	0.0
14	0.5951722824027789	0.0	0.0	0.0	0.0
15	0.6164468907969086	0.0	0.0	0.0	0.0
16	0.6341139808107322	0.0	0.0	0.0	0.0
17	0.6614433700539705	0.0	0.0	0.0	0.0
18	0.6944180380599039	0.0	0.0	0.0	0.0
19	0.7281727100399576	0.0	0.0	0.0	0.0
20	0.7617421310761578	0.0	0.0	0.0	0.0
21	0.8098293760806712	0.0	0.0	0.0	0.0
22	0.8490733260285961	0.0	0.0	0.0	0.0
23	0.8904232867066457	0.0	0.0	0.0	0.0
24	0.9270152231425619	0.0	0.0	0.0	0.0
25	0.9683456837212585	0.0	0.0	0.0	0.0
26	1.017407933693422	0.0	0.0	0.0	0.0
27	1.0582118915895877	0.0	0.0	0.0	0.0
28	1.1003808564404636	0.0	0.0	0.0	0.0
29	1.1444120807795515	0.0	0.0	0.0	0.0
30	1.1892330591424363	0.0	0.0	0.0	0.0
31	1.230193017833426	0.0	0.0	0.0	0.0
32	1.2652054462217486	0.0	0.0	0.0	0.0
33	1.2946213460957583	0.0	0.0	0.0	0.0
34	1.3163542068246838	0.0	0.0	0.0	0.0
35	1.3316617848167935	0.0	0.0	0.0	0.0
36	1.3446781011349254	0.0	0.0	0.0	0.0
37	1.3529851434593059	0.0	0.0	0.0	0.0
38	1.3591861750535619	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTTGG	2500	0.0	146.17671	44
CTATCAT	5080	0.0	144.34334	44
TTTACGC	490	0.0	111.74846	44
TCGTGCG	1950	0.0	87.11299	43
GTGTTAG	2200	0.0	85.11065	43
AGGTCGA	480	0.0	83.11206	43
CCTATCA	5115	0.0	78.87429	43
GCGATAA	90	0.0	78.64368	43
TATCATA	1560	0.0	78.594536	44
GGTGCGT	815	0.0	71.567894	44
TGTGTTA	2975	0.0	71.29952	42
CACGGAT	240	0.0	69.4379	44
ACGTGTT	310	0.0	69.11791	44
CCCTATC	6480	0.0	67.867966	42
ACGACAC	735	0.0	66.40125	44
CGTGTGA	2830	0.0	64.117455	43
TACGCTA	885	0.0	62.482555	42
AAAGTTG	3185	0.0	61.81951	43
AACGACA	1090	0.0	61.393227	43
CGTGCGC	2440	0.0	60.006054	44
>>END_MODULE