FastQCFastQC Report
Tue 24 May 2016
ERR840925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736385
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC37620.1374806542208059No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCT34150.12479969010208725No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG34040.12439769988506734No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG31890.11654061837058746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA101.6821192E-4505.7901644
TTAATCG550.0459.8092344
TCGATAT251.2754754E-8404.6321444
CTATCAT10900.0366.5818544
CGTATCG303.1684976E-8337.1934244
TATATTC457.0940587E-10280.9945444
CCTATAT900.0280.9945444
CATACAG200.0013417143252.8950844
AATCGTT401.3307908E-7252.8950844
GTGTAAT652.3646862E-11233.441644
GTATAAC452.394172E-7224.7956244
ACAGTAA404.212992E-5189.6713144
TATATTA1001.8189894E-12177.0265544
ATTCGAG1800.0168.5967344
TATAGAC300.004514893168.5967144
TAATTGG1400.0162.5754144
ATCACAA802.2115273E-8158.0594344
GTATCCG1450.0156.9693644
AATCTTG2100.0156.554144
TGATAAG651.4955549E-6155.6277344
Produced by FastQC (version 0.11.5)