##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840925.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2736385
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.190135525519985	39.0	37.0	39.0	34.0	39.0
2	37.72327797440784	39.0	37.0	39.0	35.0	39.0
3	38.04820959039024	39.0	38.0	39.0	35.0	41.0
4	38.75850693524486	40.0	38.0	41.0	35.0	41.0
5	39.11407093665548	40.0	38.0	41.0	35.0	41.0
6	39.110695315169465	40.0	38.0	41.0	35.0	41.0
7	39.036125764466625	40.0	38.0	41.0	35.0	41.0
8	38.960301638841024	40.0	38.0	41.0	35.0	41.0
9	38.86694854707945	40.0	38.0	41.0	35.0	41.0
10	38.74726911600524	40.0	38.0	41.0	35.0	41.0
11	38.68173849805491	40.0	38.0	41.0	35.0	41.0
12	38.66225732124683	40.0	38.0	41.0	35.0	41.0
13	38.563551181577154	40.0	38.0	41.0	35.0	41.0
14	38.547816919037345	40.0	38.0	41.0	35.0	41.0
15	38.559479020678744	40.0	38.0	41.0	35.0	41.0
16	38.5324539492798	40.0	38.0	41.0	35.0	41.0
17	38.53321992336605	40.0	38.0	41.0	35.0	41.0
18	38.57025418572314	40.0	38.0	41.0	35.0	41.0
19	38.66063474255267	40.0	38.0	41.0	35.0	41.0
20	38.634974245217684	40.0	38.0	41.0	35.0	41.0
21	38.53447413284315	40.0	37.0	41.0	35.0	41.0
22	38.438853450811926	40.0	37.0	41.0	34.0	41.0
23	38.28635298030065	40.0	37.0	41.0	34.0	41.0
24	38.10973565488774	40.0	36.0	41.0	34.0	41.0
25	38.026045938088814	40.0	36.0	41.0	33.0	41.0
26	37.91285731053121	40.0	35.0	41.0	33.0	41.0
27	37.780941764876836	39.0	35.0	41.0	33.0	41.0
28	37.68935274121124	39.0	35.0	41.0	33.0	41.0
29	37.49706062640693	39.0	35.0	41.0	33.0	41.0
30	37.350248660897144	39.0	35.0	41.0	33.0	41.0
31	37.20222054936332	39.0	35.0	41.0	33.0	41.0
32	37.05854569609057	38.0	35.0	41.0	33.0	41.0
33	36.96510272650035	38.0	35.0	41.0	33.0	41.0
34	36.895307464581975	38.0	35.0	40.0	33.0	41.0
35	36.74425564845089	37.0	35.0	40.0	32.0	41.0
36	36.616313045945084	37.0	35.0	40.0	32.0	41.0
37	36.542920115694756	37.0	35.0	40.0	32.0	41.0
38	36.523014361759635	37.0	35.0	40.0	33.0	41.0
39	36.41129445320425	36.0	35.0	40.0	32.0	41.0
40	36.27905898840081	36.0	35.0	40.0	32.0	41.0
41	36.160623582794116	36.0	35.0	40.0	32.0	41.0
42	36.04308577918364	35.0	35.0	40.0	32.0	41.0
43	35.87326398398558	35.0	35.0	39.0	32.0	41.0
44	35.731733348913195	35.0	34.0	39.0	31.0	41.0
45	35.5480992431915	35.0	34.0	39.0	31.0	41.0
46	35.23975569105076	35.0	34.0	39.0	30.0	41.0
47	35.12264601541049	35.0	34.0	39.0	30.0	41.0
48	35.170183950568436	35.0	34.0	39.0	30.0	41.0
49	34.97926634359254	35.0	33.0	38.0	30.0	40.0
50	36.120834809524695	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	23.0
22	56.0
23	121.0
24	303.0
25	632.0
26	1268.0
27	2909.0
28	5967.0
29	11763.0
30	21754.0
31	38423.0
32	64227.0
33	108683.0
34	203355.0
35	176553.0
36	279058.0
37	415027.0
38	456062.0
39	513212.0
40	436983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.06047577369412	17.44005320888691	43.49947101741897
2	26.563550085240202	16.79862300078388	30.222501585120515	26.415325328855406
3	23.56850370105084	16.073286471019248	30.30607900569547	30.052130822234446
4	21.6534953962984	16.678976094372686	34.49499978986875	27.172528719460164
5	22.535315754179326	19.219042642025887	32.521337457996594	25.7243041457982
6	27.02872585546259	18.648289622987992	28.556946482311517	25.7660380392379
7	29.73543562035313	18.473021888367317	22.03279143833927	29.758751052940287
8	28.915412122197715	17.40745545674311	24.599645152272068	29.077487268787106
9	27.665441814656926	17.368937485039567	25.626437800236445	29.33918290006706
10	27.60627616362464	15.884862692932463	26.42928535275555	30.079575790687347
11	30.089406278721743	16.766207971466006	25.117773997445536	28.026611752366716
12	31.190018948357046	17.504079287088622	23.581696289082128	27.724205475472203
13	28.91866458849906	17.09028517551441	26.4872084885716	27.50384174741493
14	28.141982944651428	19.360835554938358	22.06162510026915	30.43555640014106
15	26.130058453032014	21.600213420260673	23.055564184133445	29.21416394257387
16	28.135002932701358	19.679431074209223	21.881935473261255	30.303630519828168
17	29.421517805425772	20.479939774556577	20.306426179064715	29.792116240952936
18	27.39815486490388	18.315660990686617	20.832631373143766	33.453552771265734
19	29.799790599641497	18.189655329933473	22.86118364192173	29.149370428503303
20	30.64831885864014	18.473606601410257	23.1761977938046	27.701876746145004
21	30.24779042422758	21.508669284475687	19.756247750225207	28.487292541071525
22	32.087334201875834	19.569249210180583	21.617389365896976	26.726027222046607
23	31.638420763160152	20.87754464375444	22.196839991448574	25.28719460163683
24	33.30799576813936	19.98720940218573	20.955348023030385	25.74944680664453
25	31.170983283215314	21.057027251857015	22.685091285343493	25.086898179584182
26	33.40184945547584	19.779259730605776	21.580105546078045	25.238785267840335
27	31.071842693696954	20.02038095859455	22.54431320098932	26.36346314671918
28	31.49039715409412	20.462176545717654	21.398019967242874	26.649406332945354
29	32.061203415893075	21.1895676779061	22.42062005233546	24.32860885386536
30	31.57125928093891	22.070059538312183	21.152356375985235	25.206324804763668
31	29.636631510056322	21.7357138545744	22.878446048212503	25.74920858715677
32	29.31307775232191	19.959976795260236	25.32792756205298	25.399017890364874
33	28.109098632887928	18.600254885301617	24.665907159959836	28.624739321850623
34	30.185691480649012	19.012493429578896	23.97901200320127	26.82280308657082
35	27.663073197253564	19.874843769999693	23.46426839345261	28.99781463929414
36	27.714172015303205	20.222219595513057	24.709085393001658	27.35452299618208
37	27.239386622823442	22.97572811494343	24.79238465693192	24.99250060530121
38	30.304633352044036	20.47178095154886	22.046073006841574	27.177512689565532
39	28.31953078707656	22.003073183959184	20.2365347340089	29.440861294955354
40	28.109240233794115	20.466440588105982	22.293926913478078	29.13039226462183
41	27.373847827378118	20.900079815082854	22.912827447276076	28.813244910262952
42	27.893005110711584	21.044241583111077	23.187337407415757	27.87541589876158
43	27.337843198767715	21.142089808899225	24.60308370555907	26.916983286773995
44	28.327708917313675	21.81079523781098	21.98035328191131	27.881142562964033
45	29.590966707523584	19.943819457904176	23.25127370770649	27.213940126865747
46	27.760416041374462	22.41113368977816	23.249073088589398	26.579377180257975
47	27.72544982311474	21.186077849312817	23.699964486834762	27.388507840737685
48	26.317394687557698	22.316576982933714	21.057377805913852	30.308650523594732
49	25.331108187040662	23.398055136557655	23.71167238999968	27.559164286402005
50	23.664901452572302	27.395159335434187	29.75955173506871	19.1803874769248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12965.0
1	11742.5
2	17614.0
3	23510.0
4	20302.0
5	13125.5
6	6187.5
7	5460.5
8	7538.0
9	8898.0
10	8754.5
11	7751.5
12	6890.5
13	6056.0
14	5325.0
15	4904.5
16	4497.0
17	4527.0
18	5504.0
19	7522.5
20	9359.0
21	9363.0
22	8388.5
23	8884.5
24	11284.0
25	14007.5
26	15426.5
27	16643.0
28	18345.0
29	18711.0
30	19014.5
31	21040.0
32	25851.0
33	30359.5
34	32603.0
35	34054.5
36	35591.5
37	39038.0
38	44023.5
39	50227.0
40	54733.5
41	59313.5
42	66357.5
43	68019.5
44	69667.0
45	74010.5
46	83260.5
47	100494.0
48	112702.0
49	115463.5
50	128557.0
51	149454.5
52	166577.0
53	177185.0
54	178993.0
55	183144.0
56	188209.5
57	196975.0
58	214810.5
59	223129.5
60	215360.0
61	205664.0
62	199556.5
63	192695.0
64	183689.0
65	179642.0
66	175179.0
67	169901.5
68	161239.0
69	147551.0
70	132628.5
71	124362.0
72	119821.5
73	106061.5
74	91391.5
75	82776.0
76	74286.0
77	58034.0
78	43359.0
79	37402.0
80	31589.5
81	22275.0
82	14507.5
83	10664.5
84	9389.0
85	9032.5
86	7565.5
87	5550.5
88	2978.0
89	1263.0
90	893.0
91	692.0
92	549.5
93	496.0
94	374.0
95	216.0
96	171.0
97	165.0
98	137.5
99	110.0
100	110.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	14809.0
25	12238.0
26	11722.0
27	13620.0
28	15060.0
29	17534.0
30	17189.0
31	19230.0
32	25583.0
33	19169.0
34	15504.0
35	16697.0
36	14493.0
37	16950.0
38	17312.0
39	14619.0
40	16474.0
41	19187.0
42	29167.0
43	47805.0
44	164109.0
45	282143.0
46	299469.0
47	403150.0
48	434177.0
49	564459.0
50	214516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.617235991768743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6522846966534	9.620888340719604
2	11.32736707693735	3.084948613040951
3	4.210634146486748	1.7201159654312939
4	2.273778057347254	1.2385028959921214
5	1.3779926932022715	0.9382225849134156
6	0.9348859358486891	0.7638337448302303
7	0.7008457419583931	0.6680507303451588
8	0.5592277687148036	0.60921091997918
9	0.4448600074161942	0.5451987333857677
>10	3.964181926825977	12.089004408113365
>50	1.7397785586228742	17.447099578872397
>100	1.7310544116039557	39.95358854805999
>500	0.05739570330251842	5.323122655168016
>1k	0.025713275079528253	5.998212281148508
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	3762	0.1374806542208059	No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCT	3415	0.12479969010208725	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	3404	0.12439769988506734	No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGG	3189	0.11654061837058746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.02437522497747941	0.0	0.0	0.0	0.0
2	0.043890022785536394	0.0	0.0	0.0	0.0
3	0.054926481470991835	0.0	0.0	0.0	0.0
4	0.06596294015644728	0.0	0.0	0.0	0.0
5	0.07718212166782086	0.0	0.0	0.0	0.0
6	0.09384644339155492	0.0	0.0	0.0	0.0
7	0.10393274338223606	0.0	0.0	0.0	0.0
8	0.11420176619883532	0.0	0.0	0.0	0.0
9	0.1234109966251094	0.0	0.0	0.0	0.0
10	0.1315604346610583	0.0	0.0	0.0	0.0
11	0.14022149660957797	0.0	0.0	0.0	0.0
12	0.14837093464552686	0.0	0.0	0.0	0.0
13	0.1567396400725775	0.0	0.0	0.0	0.0
14	0.164340909630772	0.0	0.0	0.0	0.0
15	0.1750484672295748	0.0	0.0	0.0	0.0
16	0.18425769765584887	0.0	0.0	0.0	0.0
17	0.196207770470895	0.0	0.0	0.0	0.0
18	0.21035051719695877	0.0	0.0	0.0	0.0
19	0.22146006501278145	0.0	0.0	0.0	0.0
20	0.23450647478333642	0.0	0.0	0.0	0.0
21	0.2446658639043848	0.0	0.0	0.0	0.0
22	0.2549348867209841	0.0	0.0	0.0	0.0
23	0.26739658344860096	0.0	0.0	0.0	0.0
24	0.27792141822148564	0.0	0.0	0.0	0.0
25	0.29074856060093884	0.0	0.0	0.0	0.0
26	0.3071936149335711	0.0	0.0	0.0	0.0
27	0.3194725888352699	0.0	0.0	0.0	0.0
28	0.3344558605605571	0.0	0.0	0.0	0.0
29	0.3459673985933997	0.0	0.0	0.0	0.0
30	0.36065831379721786	0.0	0.0	0.0	0.0
31	0.37308346595965114	0.0	0.0	0.0	0.0
32	0.38726275725089854	0.0	0.0	0.0	0.0
33	0.4007111572384734	0.0	0.0	0.0	0.0
34	0.41258814092315227	0.0	0.0	0.0	0.0
35	0.42223590613162987	0.0	0.0	0.0	0.0
36	0.43221257242676014	0.0	0.0	0.0	0.0
37	0.4399600202456891	0.0	0.0	0.0	0.0
38	0.44430882350254075	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	10	1.6821192E-4	505.79016	44
TTAATCG	55	0.0	459.80923	44
TCGATAT	25	1.2754754E-8	404.63214	44
CTATCAT	1090	0.0	366.58185	44
CGTATCG	30	3.1684976E-8	337.19342	44
TATATTC	45	7.0940587E-10	280.99454	44
CCTATAT	90	0.0	280.99454	44
CATACAG	20	0.0013417143	252.89508	44
AATCGTT	40	1.3307908E-7	252.89508	44
GTGTAAT	65	2.3646862E-11	233.4416	44
GTATAAC	45	2.394172E-7	224.79562	44
ACAGTAA	40	4.212992E-5	189.67131	44
TATATTA	100	1.8189894E-12	177.02655	44
ATTCGAG	180	0.0	168.59673	44
TATAGAC	30	0.004514893	168.59671	44
TAATTGG	140	0.0	162.57541	44
ATCACAA	80	2.2115273E-8	158.05943	44
GTATCCG	145	0.0	156.96936	44
AATCTTG	210	0.0	156.5541	44
TGATAAG	65	1.4955549E-6	155.62773	44
>>END_MODULE