FastQCFastQC Report
Tue 24 May 2016
ERR840924.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840924.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17446991
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA394220.2259530024403635No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA365340.2094000048489737No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG283420.16244634963129173No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC261230.14972782412738106No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208020.11922972849587644No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA195510.1120594376417114No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG179460.1028601436201807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAT165400.0243.7787244
ACTTCTA59350.0113.27515444
ACCACTC25850.0108.3111544
AATCGCA26300.0102.7442844
CCTATCA173100.093.5446143
CCCATAC30000.086.2742544
ACATTGG24200.084.7540644
TAACTCG1851.8189894E-1279.1910544
TAATTCC26350.077.2208744
CCCTATC231100.072.4781742
AACGACA17100.071.8107143
CGATACT3700.070.39204444
TGCACCG24300.064.934243
TCATACG1808.227289E-963.3039544
CTAATTC13450.059.1310743
ATTAAGC14550.055.77657742
ATTAGTC4400.055.49372544
TACGCTA27800.054.92387842
TCGCAAA23250.053.6365943
AAACTAC38200.053.29846243
Produced by FastQC (version 0.11.5)