##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840924.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17446991
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.31585257308839	39.0	37.0	39.0	34.0	39.0
2	37.804012336568526	39.0	38.0	39.0	35.0	39.0
3	38.09975966629432	39.0	38.0	39.0	35.0	40.0
4	38.788805530993855	39.0	38.0	41.0	35.0	41.0
5	39.32362772468903	41.0	39.0	41.0	35.0	41.0
6	39.2407108480769	40.0	39.0	41.0	35.0	41.0
7	39.285503099073075	40.0	39.0	41.0	35.0	41.0
8	39.20531637805052	40.0	39.0	41.0	35.0	41.0
9	39.14258900001725	40.0	38.0	41.0	35.0	41.0
10	39.06485519480121	40.0	38.0	41.0	35.0	41.0
11	38.86986042464285	40.0	38.0	41.0	35.0	41.0
12	38.891673183072086	40.0	38.0	41.0	35.0	41.0
13	38.83806164627471	40.0	38.0	41.0	35.0	41.0
14	38.80399989889374	40.0	38.0	41.0	35.0	41.0
15	38.805519129344425	40.0	38.0	41.0	35.0	41.0
16	38.73472113328883	40.0	38.0	41.0	35.0	41.0
17	38.72605860804307	40.0	38.0	41.0	35.0	41.0
18	38.7581443699948	40.0	38.0	41.0	35.0	41.0
19	38.840105093193436	40.0	38.0	41.0	35.0	41.0
20	38.76854197953103	40.0	38.0	41.0	35.0	41.0
21	38.72096632594125	40.0	38.0	41.0	35.0	41.0
22	38.58661622511297	40.0	37.0	41.0	35.0	41.0
23	38.457780484898514	40.0	37.0	41.0	35.0	41.0
24	38.24834803892545	40.0	37.0	41.0	34.0	41.0
25	38.16431382757737	40.0	36.0	41.0	34.0	41.0
26	38.094165600916476	40.0	36.0	41.0	34.0	41.0
27	38.00286368232547	40.0	35.0	41.0	34.0	41.0
28	37.88187743828391	39.0	35.0	41.0	33.0	41.0
29	37.74591293398549	39.0	35.0	41.0	33.0	41.0
30	37.64346762464431	39.0	35.0	41.0	33.0	41.0
31	37.47402532535701	39.0	35.0	41.0	33.0	41.0
32	37.34441469525289	39.0	35.0	41.0	33.0	41.0
33	37.245517782893316	39.0	35.0	41.0	33.0	41.0
34	37.1519443760175	38.0	35.0	41.0	33.0	41.0
35	37.01540918223464	38.0	35.0	41.0	33.0	41.0
36	36.93790057078172	38.0	35.0	41.0	33.0	41.0
37	36.84693035470385	37.0	35.0	40.0	33.0	41.0
38	36.77213832290063	37.0	35.0	40.0	33.0	41.0
39	36.67049053308886	37.0	35.0	40.0	33.0	41.0
40	36.582169819009636	36.0	35.0	40.0	33.0	41.0
41	36.46948876065926	36.0	35.0	40.0	33.0	41.0
42	36.360064088300696	36.0	35.0	40.0	33.0	41.0
43	36.24755801638739	35.0	35.0	40.0	32.0	41.0
44	36.08387131235651	35.0	35.0	40.0	32.0	41.0
45	35.94835567067451	35.0	35.0	40.0	31.0	41.0
46	35.69236816220206	35.0	34.0	39.0	31.0	41.0
47	35.544470300789065	35.0	34.0	39.0	31.0	41.0
48	35.548425141443424	35.0	34.0	39.0	31.0	41.0
49	35.5580616603525	35.0	34.0	39.0	31.0	41.0
50	36.325226775469915	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	53.0
21	177.0
22	431.0
23	886.0
24	1782.0
25	4015.0
26	7609.0
27	15661.0
28	31763.0
29	62790.0
30	116339.0
31	204796.0
32	347109.0
33	608176.0
34	1185555.0
35	1001036.0
36	1685104.0
37	2604057.0
38	2815490.0
39	3226386.0
40	3527765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.16498942425086	18.669293748131125	40.16571682761801
2	26.09412706179535	20.359138145941614	29.378991483402494	24.167743308860537
3	25.4638923124337	17.59444938098495	28.286430594249744	28.655227712331598
4	22.65192318835953	20.81987661941248	31.827058316244905	24.701141875983083
5	24.26412669095777	20.12692618457819	28.709804458545317	26.899142665918724
6	23.386611479308954	22.087155315205926	27.692964362737392	26.833268842747728
7	30.719325756515836	19.046023466166744	20.868538305545066	29.366112471772354
8	26.45170734598304	21.191923581550537	22.84048865503513	29.515880417431294
9	27.957904030557472	21.2229260621502	24.745682507659915	26.073487399632405
10	29.84178188663019	18.290311492680885	23.318863407449456	28.54904321323946
11	27.647907882797668	20.31005231790399	24.97016247672736	27.07187732257098
12	28.804898219985326	21.481595307752492	22.912879361260632	26.800627111001546
13	29.510005478881716	20.457252485543208	23.559162723245517	26.47357931232956
14	26.045488302252235	21.72109219291739	21.534882433308987	30.698537071521386
15	25.125203537962506	24.491392240644817	22.254720025934557	28.128684195458113
16	26.3123709985292	23.485476664715424	20.19674338113661	30.00540895561877
17	26.548021948311888	27.29965298887355	18.800370791731368	27.35195427108319
18	26.540651049799934	22.773239236496426	18.307242778998393	32.37886693470525
19	30.033511222651516	21.954169633032997	19.918483364839243	28.093835779476244
20	27.907832359172996	22.23134063633093	22.33917011821695	27.52165688627913
21	28.300582031595017	25.584606537597228	18.50589594503717	27.608915485770584
22	30.70993158648388	23.32856135479178	21.452558782199176	24.50894827652516
23	31.843915091146663	22.84565286931139	20.255177526027268	25.05525451351468
24	30.789074173305874	23.021918220740755	22.774230811490646	23.414776794462725
25	28.7127933470065	25.563966273672662	20.247757156786914	25.475483222533924
26	30.214868570034202	25.759056123520956	20.837553588180214	23.188521718264628
27	29.58785214330854	25.531182431918808	20.22747523839797	24.65349018637468
28	28.900014456029112	23.528004150502195	19.78855320208071	27.783428191387987
29	28.61620195995906	26.313037182759224	19.449770800648654	25.620990056633065
30	29.864980959963184	25.39295738913106	19.361034116835853	25.381027534069904
31	27.4519752002555	25.56433525771483	21.226599859932143	25.757089682097522
32	27.301040758448387	24.487822713428656	23.088131494377116	25.123005033745844
33	26.31255121316187	22.837235359174773	22.381493856176757	28.468719571486595
34	28.12330658552935	24.601547294052896	22.826951086398665	24.448195034019086
35	25.394246876057146	23.51876090603136	21.26781312061215	29.81917909729934
36	25.95695578799931	23.555802963995365	25.256196145037922	25.231045102967403
37	24.768861402846767	26.83694799691052	24.294791090546013	24.0993995096967
38	25.931256028505985	24.022533363172286	23.551495581651245	26.49471502667049
39	27.45263400905687	25.4944700383183	21.27892890832453	25.7739670443003
40	26.657422589448565	24.674219364192428	22.31366390453825	26.354694141820755
41	27.022119451354147	23.93071771690433	21.346002735381074	27.70116009636045
42	24.394492348135564	27.00764025330408	22.006651859739847	26.591215538820517
43	27.03378564504004	24.27445603621794	21.791634551230572	26.900123767511445
44	26.67900415378286	26.290154340238608	20.351678101734503	26.679163404244026
45	26.634263973467643	22.7700567599172	23.872971275708753	26.722707990906404
46	25.046854050986262	25.670950328966775	22.2450328169663	27.037162803080665
47	26.507396264424777	23.566699903374083	23.5010758989238	26.42482793327734
48	24.072579938862376	25.88369596927777	20.222467986100582	29.821256105759268
49	25.049000621828792	28.008238773461947	19.957736162110802	26.98502444259846
50	19.510554913523457	33.19982519145586	25.43431352326809	21.855306371752597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	307586.0
1	246194.5
2	167670.5
3	138362.5
4	111755.0
5	78876.0
6	50012.0
7	45170.5
8	55643.5
9	61208.5
10	60242.0
11	58983.0
12	60014.5
13	58538.0
14	56172.0
15	56941.0
16	58678.5
17	61426.0
18	66911.5
19	74819.0
20	82573.5
21	89321.5
22	94344.5
23	104219.5
24	115081.0
25	123506.0
26	136918.5
27	148598.0
28	153359.5
29	160478.5
30	176956.0
31	210339.0
32	261264.0
33	294021.0
34	313391.0
35	327315.5
36	330527.5
37	334714.0
38	337097.5
39	381650.5
40	450302.0
41	500136.0
42	532315.0
43	523177.0
44	515507.0
45	540922.5
46	606040.0
47	675613.5
48	745934.0
49	865657.0
50	1006814.5
51	1091643.5
52	1103268.5
53	1036856.0
54	989691.0
55	1010994.0
56	1006590.0
57	1011664.0
58	1033487.5
59	1037829.5
60	1058432.5
61	1091624.5
62	1077579.0
63	1032800.0
64	993413.5
65	969935.5
66	992685.0
67	1032440.5
68	1010694.5
69	936159.5
70	822311.0
71	714280.0
72	653532.5
73	615505.0
74	570230.5
75	510149.0
76	439509.0
77	341654.5
78	268992.5
79	229973.5
80	188495.5
81	146312.0
82	110616.0
83	87099.0
84	70471.5
85	56629.5
86	42655.0
87	31899.0
88	20626.0
89	11783.0
90	7605.5
91	4750.5
92	3263.0
93	2516.0
94	1865.0
95	916.0
96	484.5
97	429.0
98	327.5
99	226.0
100	226.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	106947.0
25	86939.0
26	85698.0
27	88028.0
28	98310.0
29	115818.0
30	107659.0
31	112135.0
32	132368.0
33	116852.0
34	105366.0
35	92735.0
36	82805.0
37	104938.0
38	104677.0
39	102444.0
40	130867.0
41	126978.0
42	193256.0
43	281571.0
44	880833.0
45	1644427.0
46	1910512.0
47	2356783.0
48	2819951.0
49	3334628.0
50	2123466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.464077855904808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3206822270944	12.455499471502227
2	13.170155114138208	4.600092285753051
3	4.457088381312618	2.3351681550567682
4	2.2005155953421056	1.53719902720749
5	1.2332378784040323	1.0768681161649445
6	0.8402079324982153	0.8804074048978587
7	0.6552213463503987	0.8009985623859892
8	0.4732408381852534	0.6611771874128732
9	0.3785096170067836	0.594928927855366
>10	3.1937043949515456	12.877105487627388
>50	1.2256997173416	15.474675523804507
>100	0.7728191230677962	22.999337850254193
>500	0.04369577371738147	5.244805285727201
>1k	0.031036250490629733	10.17252916686743
>5k	0.002450230301891821	2.7198146004322066
>10k+	0.001735579797173373	5.569392947050428
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	39422	0.2259530024403635	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36534	0.2094000048489737	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG	28342	0.16244634963129173	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	26123	0.14972782412738106	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20802	0.11922972849587644	No Hit
AGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCA	19551	0.1120594376417114	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	17946	0.1028601436201807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03480829445031525	0.0	0.0	0.0	0.0
2	0.07382361806686322	0.0	0.0	0.0	0.0
3	0.09499632343479744	0.0	0.0	0.0	0.0
4	0.11774523182822758	0.0	0.0	0.0	0.0
5	0.13566236149259206	0.0	0.0	0.0	0.0
6	0.16313414731514447	0.0	0.0	0.0	0.0
7	0.18194541396851754	0.0	0.0	0.0	0.0
8	0.20195459492126752	0.0	0.0	0.0	0.0
9	0.22293242427877677	0.0	0.0	0.0	0.0
10	0.24169783775322634	0.0	0.0	0.0	0.0
11	0.2585316860655227	0.0	0.0	0.0	0.0
12	0.27790465416070886	0.0	0.0	0.0	0.0
13	0.298372366902694	0.0	0.0	0.0	0.0
14	0.3150056075572	0.0	0.0	0.0	0.0
15	0.33648782188286797	0.0	0.0	0.0	5.731647365439691E-6
16	0.3547431187417934	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
17	0.379440787239473	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
18	0.408901454697833	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
19	0.42716248320412387	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
20	0.44771043900922514	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
21	0.46514611029489267	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
22	0.4828683639488322	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
23	0.5012612203445281	5.731647365439691E-6	0.0	0.0	5.731647365439691E-6
24	0.5181523851304789	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
25	0.53667706941558	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
26	0.5580331874992083	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
27	0.5740588735329777	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
28	0.5911277193872571	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
29	0.6096638669670891	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
30	0.6281025765417085	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
31	0.6477334687683395	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
32	0.6665390037743472	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
33	0.6841638194230741	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
34	0.6992036621099879	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
35	0.7119451142033604	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
36	0.7243312041600755	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
37	0.7336164728920878	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
38	0.7402078673623435	5.731647365439691E-6	0.0	0.0	1.1463294730879381E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	16540	0.0	243.77872	44
ACTTCTA	5935	0.0	113.275154	44
ACCACTC	2585	0.0	108.31115	44
AATCGCA	2630	0.0	102.74428	44
CCTATCA	17310	0.0	93.54461	43
CCCATAC	3000	0.0	86.27425	44
ACATTGG	2420	0.0	84.75406	44
TAACTCG	185	1.8189894E-12	79.19105	44
TAATTCC	2635	0.0	77.22087	44
CCCTATC	23110	0.0	72.47817	42
AACGACA	1710	0.0	71.81071	43
CGATACT	370	0.0	70.392044	44
TGCACCG	2430	0.0	64.9342	43
TCATACG	180	8.227289E-9	63.30395	44
CTAATTC	1345	0.0	59.13107	43
ATTAAGC	1455	0.0	55.776577	42
ATTAGTC	440	0.0	55.493725	44
TACGCTA	2780	0.0	54.923878	42
TCGCAAA	2325	0.0	53.63659	43
AAACTAC	3820	0.0	53.298462	43
>>END_MODULE