FastQCFastQC Report
Tue 24 May 2016
ERR840923.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840923.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13560087
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA968980.7145824359386485No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA550270.4058012312162894No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT245550.18108290898133617No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT214880.15846505999555904No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165150.12179125399416685No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT157520.11616444643754867No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG143050.1054934234566489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA323600.0179.3399844
CTATCAT227450.0160.6598744
TTAAGCC311350.0106.08597643
ACTTCTA81350.0104.9216744
ACATTGG29800.097.7690744
CGACGGG81150.084.3211944
ACCACTC36150.072.9330344
ATTAAGC430300.072.43764542
AATCGCA24750.070.4649544
CCTATCA320350.065.4544543
TCGCAAA15900.064.69131543
CTAATTC26250.062.38865743
CAGGCAC55850.061.3511544
CGGTTAA903.45608E-1157.46323843
ACCTTAG96250.056.5672142
GATTAAG480000.056.03147541
CGAATAT1301.6807462E-955.24005544
TAATTCC52600.055.19505344
CCCTATC396150.054.89850642
CCTTAGC151900.053.45329343
Produced by FastQC (version 0.11.5)