##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840923.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13560087
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.40737976091156	39.0	38.0	39.0	35.0	39.0
2	37.80877932420345	39.0	38.0	39.0	35.0	39.0
3	38.04416719450251	39.0	38.0	39.0	35.0	40.0
4	38.56176424236806	39.0	38.0	41.0	35.0	41.0
5	39.31446007684169	41.0	39.0	41.0	35.0	41.0
6	39.30394974604514	41.0	39.0	41.0	35.0	41.0
7	39.29447790416094	41.0	39.0	41.0	35.0	41.0
8	39.15926136757087	40.0	38.0	41.0	35.0	41.0
9	39.10122752162283	40.0	38.0	41.0	35.0	41.0
10	39.055402078172506	40.0	38.0	41.0	35.0	41.0
11	38.958370989802646	40.0	38.0	41.0	35.0	41.0
12	38.95327470981565	40.0	38.0	41.0	35.0	41.0
13	38.836162186865025	40.0	38.0	41.0	35.0	41.0
14	38.83866165460443	40.0	38.0	41.0	35.0	41.0
15	38.844798193403925	40.0	38.0	41.0	35.0	41.0
16	38.808446509229626	40.0	38.0	41.0	35.0	41.0
17	38.81347405809417	40.0	38.0	41.0	35.0	41.0
18	38.848206947344806	40.0	38.0	41.0	35.0	41.0
19	38.863752127844016	40.0	38.0	41.0	35.0	41.0
20	38.84303345546382	40.0	38.0	41.0	35.0	41.0
21	38.78780903101875	40.0	38.0	41.0	35.0	41.0
22	38.75757456423399	40.0	38.0	41.0	35.0	41.0
23	38.66775994873779	40.0	38.0	41.0	35.0	41.0
24	38.491503704954106	40.0	37.0	41.0	35.0	41.0
25	38.4571178530837	40.0	37.0	41.0	35.0	41.0
26	38.41327614418939	40.0	37.0	41.0	34.0	41.0
27	38.33014271927372	40.0	36.0	41.0	34.0	41.0
28	38.284880058613396	40.0	36.0	41.0	34.0	41.0
29	38.18385372660692	40.0	36.0	41.0	34.0	41.0
30	38.08528016625072	40.0	35.0	41.0	34.0	41.0
31	37.992579215759235	40.0	35.0	41.0	33.0	41.0
32	37.89253826087607	40.0	35.0	41.0	33.0	41.0
33	37.79553955757766	39.0	35.0	41.0	33.0	41.0
34	37.67072460647404	39.0	35.0	41.0	33.0	41.0
35	37.57107134112541	39.0	35.0	41.0	33.0	41.0
36	37.49353489069579	39.0	35.0	41.0	33.0	41.0
37	37.377048313551654	39.0	35.0	41.0	33.0	41.0
38	37.29707974230469	38.0	35.0	41.0	33.0	41.0
39	37.21953652381143	38.0	35.0	41.0	33.0	41.0
40	37.144426432548634	38.0	35.0	41.0	33.0	41.0
41	37.05997846322374	37.0	35.0	41.0	33.0	41.0
42	36.97652822183545	37.0	35.0	41.0	33.0	41.0
43	36.880054141712044	37.0	35.0	41.0	33.0	41.0
44	36.758099183745486	37.0	35.0	40.0	33.0	41.0
45	36.594079334083844	37.0	35.0	40.0	32.0	41.0
46	36.37316677238376	37.0	35.0	40.0	32.0	41.0
47	36.240014235707626	36.0	35.0	40.0	31.0	41.0
48	36.194313135534664	36.0	35.0	40.0	31.0	41.0
49	36.389388286793164	37.0	35.0	40.0	32.0	41.0
50	36.56195684817353	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	11.0
20	38.0
21	138.0
22	351.0
23	695.0
24	1572.0
25	3298.0
26	5786.0
27	11380.0
28	22497.0
29	43460.0
30	80879.0
31	146349.0
32	247076.0
33	439360.0
34	923726.0
35	735982.0
36	1029814.0
37	1604953.0
38	2019982.0
39	2635348.0
40	3607387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.53541006042218	20.10161144246346	40.36297849711436
2	23.410602011624263	22.502333502727527	34.09168392503676	19.995380560611448
3	24.654849190864336	18.597107820915898	25.549467344862904	31.19857564335686
4	19.595722357828528	21.416662002242315	30.822132630859965	28.16548300906919
5	20.660000190264267	19.44604042732174	33.212205791894995	26.681753590518998
6	24.325374903568097	23.96930049195112	24.07583373174523	27.629490872735552
7	29.76775149009	19.130555725785538	19.90456255922252	31.197130224901947
8	23.696979230295497	22.797958449676614	25.985216761514877	27.519845558513012
9	23.69539369474547	23.400874935389425	28.093057220060608	24.810674149804495
10	31.86106401824708	17.951728480798096	21.476786985216243	28.710420515738576
11	25.238746624560743	23.919492551928318	22.878599525209538	27.963161298301404
12	24.95520862071165	21.45360129326604	25.554010088578337	28.037179997443967
13	26.41617269859699	19.830425866736697	22.368949402758258	31.384452031908054
14	21.597545797456906	21.398387783205226	21.791032756648242	35.213033662689625
15	24.90475171730093	23.907862833033448	25.271851131928578	25.91553431773705
16	27.036463704104552	24.190965736429273	19.4162766064849	29.35629395298128
17	24.43415001688411	26.30981644881777	18.78417889206758	30.47185464223054
18	24.49824252602509	23.908423301413922	18.860697575170423	32.73263659739057
19	25.092715113111	26.031632392919015	21.449648516266894	27.426003977703097
20	29.284981726149695	20.092393212521422	23.486751965529425	27.135873095799457
21	25.573936214421046	26.55853904182178	22.172711723752215	25.694813020004958
22	26.822165668996078	25.99102055908638	20.521040904826055	26.665772867091487
23	32.14572295885712	21.390555975046475	20.640582910714365	25.82313815538204
24	26.603649371866123	22.85548020451491	22.683195174190253	27.85767524942871
25	25.717997429840455	25.846379295341865	20.933150555222266	27.502472719595417
26	27.81132438293242	22.60262092250115	26.27293124723836	23.313123447328074
27	25.011040581433004	30.263659323643033	18.50214604688142	26.22315404804254
28	24.31892640708485	21.55056999036485	24.85311589157842	29.277387710971887
29	30.002799316418677	25.742146366452655	20.34209333152836	23.91296098560031
30	26.223232606893777	27.004181220183156	20.073864341793918	26.698721831129152
31	25.708858115984402	24.15291441850222	24.419983065198984	25.718244400314394
32	22.710473175823317	24.237824356980497	26.045104682599646	27.006597784596536
33	27.627378209080174	21.3431222559126	22.621806286407274	28.40769324859995
34	25.753920353399284	24.739015762046172	25.53394944136842	23.973114443186127
35	23.379857204646413	22.741168351607925	21.424686295175857	32.45428814856981
36	23.062585522530664	22.93212392049871	29.43703255680553	24.5682580001651
37	22.19714067193489	27.03148807694224	21.158527930758	29.612843320364874
38	24.439816848585703	26.294795190445306	20.749262798319425	28.51612516264956
39	23.24951661861482	28.55210711221715	21.719422377253267	26.478953891914763
40	23.214977746991206	28.576951188558287	23.41396024600286	24.794110818447653
41	26.915271956299385	23.777155597117382	20.140820958957182	29.16675148762605
42	24.6622163995971	27.337760354385537	21.30656500623647	26.69345823978089
43	23.469280012820633	21.908152639286065	25.918557652779878	28.704009695113424
44	24.02423817323902	23.089260856380932	23.399885376813355	29.486615593566697
45	23.96363162176135	26.3214467248721	26.45691124368555	23.258010409681
46	23.183384155041146	30.810531459466606	21.338949117635362	24.667135267856885
47	27.722650307682507	22.827513296973702	23.865448175790704	25.584388219553084
48	22.374150192231802	21.925650943453824	20.194759928091376	35.505438936223
49	23.338080016394326	25.28958826058912	19.158430310685873	32.21390141233068
50	18.719146112480825	34.10127743242902	24.724785490001466	22.45479096508869
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157561.0
1	119387.0
2	86864.0
3	80436.0
4	56485.5
5	38512.0
6	25940.5
7	20958.5
8	23340.0
9	25274.5
10	26158.0
11	24642.0
12	21772.5
13	19660.5
14	20497.0
15	22581.0
16	24173.5
17	25965.0
18	27749.5
19	31375.0
20	35773.0
21	39922.5
22	42999.5
23	50229.0
24	62466.5
25	76001.5
26	90101.0
27	102058.5
28	109047.5
29	116556.0
30	134249.5
31	179439.0
32	250666.0
33	279661.0
34	282681.5
35	310792.0
36	328882.0
37	335312.5
38	342735.0
39	367720.5
40	461088.5
41	595914.5
42	628976.0
43	759245.5
44	916592.0
45	758770.5
46	614227.0
47	592813.5
48	602431.0
49	730361.0
50	876452.0
51	936953.5
52	918610.5
53	830798.5
54	780074.0
55	762319.5
56	727181.0
57	726267.5
58	713008.5
59	698313.0
60	720448.0
61	762558.0
62	769228.5
63	750984.0
64	726890.0
65	678003.5
66	598270.5
67	539883.5
68	530762.5
69	520904.5
70	485537.5
71	472411.0
72	473632.5
73	427705.5
74	360775.0
75	322921.5
76	282532.5
77	212802.5
78	173727.5
79	163028.5
80	136287.0
81	93350.0
82	59605.5
83	43962.0
84	35477.0
85	28417.5
86	20764.5
87	14969.5
88	9503.5
89	5758.0
90	4145.0
91	3142.5
92	2381.5
93	1803.5
94	1250.5
95	669.0
96	515.0
97	443.0
98	377.5
99	312.5
100	313.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	79990.0
25	52840.0
26	47074.0
27	46712.0
28	51657.0
29	56488.0
30	57176.0
31	67692.0
32	85202.0
33	67635.0
34	74373.0
35	56450.0
36	50442.0
37	61895.0
38	71579.0
39	68963.0
40	86657.0
41	88043.0
42	133973.0
43	205328.0
44	554657.0
45	1086979.0
46	1633165.0
47	1568193.0
48	2444300.0
49	2108785.0
50	2653839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.546674690267587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53258058375867	12.167196900553416
2	12.652284077094707	4.187064574250772
3	4.097047602328995	2.0337754159883605
4	1.8528781414280038	1.2263588738766718
5	1.0092360255345025	0.8349750100109004
6	0.6965898561305784	0.6915747445159791
7	0.48118064132619887	0.5573357677495259
8	0.3628123142317756	0.4802669869773066
9	0.2688863634402645	0.4004257666045605
>10	2.7863221352325946	11.061955468923196
>50	1.2933323700412331	15.52921743262043
>100	0.8825877419903726	24.80222560924051
>500	0.05102541151881918	5.631331987617047
>1k	0.028399464515061437	8.996632693861429
>5k	0.0026526972349233215	2.946661729030758
>10k+	0.0021845741934662646	8.453001038179126
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	96898	0.7145824359386485	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	55027	0.4058012312162894	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	24555	0.18108290898133617	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	21488	0.15846505999555904	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16515	0.12179125399416685	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	15752	0.11616444643754867	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	14305	0.1054934234566489	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.015073649601215685	0.0	0.0	0.0	0.0
2	0.029387717055207683	0.0	0.0	0.0	0.0
3	0.0396531379186579	0.0	0.0	0.0	0.0
4	0.050420030490954816	0.0	0.0	0.0	0.0
5	0.05996274212694948	0.0	0.0	0.0	0.0
6	0.07646706101516901	0.0	0.0	0.0	0.0
7	0.08724870275537318	0.0	0.0	0.0	0.0
8	0.10124566309935917	0.0	0.0	0.0	0.0
9	0.1119904319197952	0.0	0.0	0.0	0.0
10	0.12218210694370914	0.0	0.0	0.0	0.0
11	0.13199767818598804	0.0	0.0	0.0	0.0
12	0.14386338376737554	0.0	0.0	0.0	0.0
13	0.1531184866291787	0.0	0.0	0.0	0.0
14	0.16298567995913302	0.0	0.0	0.0	0.0
15	0.17445315800702457	0.0	0.0	0.0	0.0
16	0.18457108719140225	0.0	0.0	0.0	0.0
17	0.19923176009121477	0.0	0.0	0.0	1.4749167907256052E-5
18	0.23501324143421792	0.0	0.0	0.0	1.4749167907256052E-5
19	0.24898070344238943	0.0	0.0	0.0	1.4749167907256052E-5
20	0.2601384489642286	0.0	0.0	0.0	1.4749167907256052E-5
21	0.27235075999143665	0.0	0.0	0.0	1.4749167907256052E-5
22	0.2831545254835017	0.0	0.0	0.0	1.4749167907256052E-5
23	0.2945335085239497	0.0	0.0	0.0	1.4749167907256052E-5
24	0.3064213378571981	0.0	0.0	0.0	1.4749167907256052E-5
25	0.31888438473882946	0.0	0.0	0.0	1.4749167907256052E-5
26	0.3361040382705509	0.0	0.0	0.0	1.4749167907256052E-5
27	0.35097857410501865	0.0	0.0	0.0	1.4749167907256052E-5
28	0.3636038618336298	0.0	0.0	0.0	1.4749167907256052E-5
29	0.37795480220739	0.0	0.0	0.0	1.4749167907256052E-5
30	0.3920255083909122	0.0	0.0	0.0	1.4749167907256052E-5
31	0.4043041906737029	0.0	0.0	0.0	1.4749167907256052E-5
32	0.4177775555569813	0.0	0.0	0.0	1.4749167907256052E-5
33	0.42950314404324985	0.0	0.0	0.0	1.4749167907256052E-5
34	0.4419661909248812	0.0	0.0	0.0	1.4749167907256052E-5
35	0.4526150901539201	0.0	0.0	0.0	1.4749167907256052E-5
36	0.46154571132176364	0.0	0.0	0.0	1.4749167907256052E-5
37	0.46846307107026675	0.0	0.0	0.0	1.4749167907256052E-5
38	0.4741415007145603	0.0	0.0	0.0	1.4749167907256052E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	32360	0.0	179.33998	44
CTATCAT	22745	0.0	160.65987	44
TTAAGCC	31135	0.0	106.085976	43
ACTTCTA	8135	0.0	104.92167	44
ACATTGG	2980	0.0	97.76907	44
CGACGGG	8115	0.0	84.32119	44
ACCACTC	3615	0.0	72.93303	44
ATTAAGC	43030	0.0	72.437645	42
AATCGCA	2475	0.0	70.46495	44
CCTATCA	32035	0.0	65.45445	43
TCGCAAA	1590	0.0	64.691315	43
CTAATTC	2625	0.0	62.388657	43
CAGGCAC	5585	0.0	61.35115	44
CGGTTAA	90	3.45608E-11	57.463238	43
ACCTTAG	9625	0.0	56.56721	42
GATTAAG	48000	0.0	56.031475	41
CGAATAT	130	1.6807462E-9	55.240055	44
TAATTCC	5260	0.0	55.195053	44
CCCTATC	39615	0.0	54.898506	42
CCTTAGC	15190	0.0	53.453293	43
>>END_MODULE