FastQCFastQC Report
Tue 24 May 2016
ERR840922.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840922.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3562871
Sequences flagged as poor quality0
Sequence length24-50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC132470.3718068939346948No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC113390.31825457615501657No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC101570.2850790836940209No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA92760.2603518342370521No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT80320.22543617212074196No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC69200.1942253873351014No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT66030.18532806829099344No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA62670.17589747144928908No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59070.165793260547463No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG52620.14768988268169125No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT50780.1425255082207579No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG45040.12641490528284635No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG43910.12324330574977314No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT41490.11645103064354562No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA40640.11406531418061444No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT39270.11022010058741953No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA39100.10974295729483328No Hit
CCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCCCTTGAG38070.10685203028681083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAT152.7036149E-6377.4944244
GATATTG201.805347E-8377.494444
GATAGTA500.0339.7449644
TAAGCCA32400.0286.0335744
CTATCAT19750.0269.5023244
GTTCTAA502.910383E-11226.4966444
CCGATTG1300.0203.2662244
TATTGCG650.0203.2662244
ATAACTA650.0203.2662244
AAGTCGA650.0203.2662244
GATTAAC200.0032217682188.747244
TAACTAT701.8189894E-12188.747244
TATGTTA950.0178.8131344
CGAATCC1000.0169.8724844
ACTTCTA5450.0159.3095744
CCAGAAT5100.0159.1397944
CGACGGG14450.0138.4581544
TACGATG100.009294534132.4498343
TCAAGTA352.3646862E-11132.4498343
ATAATCT1150.0131.302444
Produced by FastQC (version 0.11.5)