##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840922.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3562871
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.15663182865728	39.0	37.0	39.0	34.0	39.0
2	37.68992983467546	39.0	37.0	39.0	35.0	39.0
3	37.9388577357979	39.0	38.0	39.0	35.0	40.0
4	38.632428173795795	39.0	38.0	41.0	35.0	41.0
5	39.0618043706887	40.0	38.0	41.0	35.0	41.0
6	39.05443054211056	40.0	38.0	41.0	35.0	41.0
7	38.91969117040723	40.0	38.0	41.0	35.0	41.0
8	38.87211886144629	40.0	38.0	41.0	35.0	41.0
9	38.757147255682284	40.0	38.0	41.0	35.0	41.0
10	38.64308278351925	40.0	38.0	41.0	35.0	41.0
11	38.536993621155524	40.0	38.0	41.0	34.0	41.0
12	38.440026315855945	40.0	38.0	41.0	34.0	41.0
13	38.31153246918005	40.0	37.0	41.0	34.0	41.0
14	38.3071006500095	40.0	37.0	41.0	34.0	41.0
15	38.305570142730396	40.0	37.0	41.0	34.0	41.0
16	38.22286633448138	40.0	37.0	41.0	34.0	41.0
17	38.22243466013785	40.0	37.0	41.0	34.0	41.0
18	38.2501173912836	40.0	37.0	41.0	34.0	41.0
19	38.28720293269108	40.0	37.0	41.0	34.0	41.0
20	38.246139138913534	40.0	36.0	41.0	34.0	41.0
21	38.16208361178387	40.0	36.0	41.0	34.0	41.0
22	38.04887631351233	40.0	36.0	41.0	34.0	41.0
23	37.88358489543966	40.0	35.0	41.0	33.0	41.0
24	37.70033689123182	39.0	35.0	41.0	33.0	41.0
25	37.567481918608166	39.0	35.0	41.0	33.0	41.0
26	37.45112722567726	39.0	35.0	41.0	33.0	41.0
27	37.287466290043774	39.0	35.0	41.0	33.0	41.0
28	37.20773271435541	38.0	35.0	41.0	33.0	41.0
29	36.98373818828896	38.0	35.0	41.0	33.0	41.0
30	36.83261944454996	38.0	35.0	40.0	33.0	41.0
31	36.691101842815	37.0	35.0	40.0	32.0	41.0
32	36.49697836799863	37.0	35.0	40.0	32.0	41.0
33	36.36480271264193	37.0	35.0	40.0	32.0	41.0
34	36.25368200144085	36.0	35.0	40.0	32.0	41.0
35	36.089498944677175	36.0	35.0	40.0	31.0	41.0
36	35.9570383337605	35.0	35.0	39.0	31.0	41.0
37	35.93783776887861	35.0	35.0	39.0	32.0	41.0
38	35.83202789719277	35.0	35.0	39.0	32.0	41.0
39	35.73627997172065	35.0	35.0	39.0	32.0	41.0
40	35.603095438444946	35.0	34.0	39.0	31.0	41.0
41	35.51158733021699	35.0	34.0	39.0	31.0	41.0
42	35.43529820583503	35.0	34.0	38.0	31.0	41.0
43	35.319581150626966	35.0	34.0	38.0	31.0	41.0
44	35.148792465536225	35.0	34.0	37.0	31.0	41.0
45	34.9232478650974	35.0	34.0	37.0	30.0	40.0
46	34.61720809577427	35.0	33.0	37.0	30.0	40.0
47	34.53318750761797	35.0	33.0	37.0	30.0	40.0
48	34.61117093594727	35.0	33.0	37.0	30.0	40.0
49	34.56845874500988	35.0	33.0	37.0	30.0	40.0
50	34.95322012261514	35.0	33.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	14.0
21	27.0
22	95.0
23	231.0
24	456.0
25	1019.0
26	2103.0
27	4465.0
28	9178.0
29	18128.0
30	33316.0
31	58676.0
32	97324.0
33	168118.0
34	313439.0
35	295153.0
36	428999.0
37	585300.0
38	600664.0
39	549235.0
40	396930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.45972222962886	18.56651559935793	41.973762171013206
2	27.641360015560483	16.97611280341051	29.38192261241005	26.000604568618957
3	23.588280350312992	16.588784718840508	30.18630200195292	29.63663292889358
4	20.23966065569031	17.740889299668723	34.17499538995378	27.84445465468719
5	23.531135424212664	17.74170324999137	32.91118314415537	25.81597818164059
6	28.363446220758483	18.885303453310545	25.476167955561678	27.27508237036929
7	31.26029541905952	16.666643277289577	21.813756377932293	30.25930492571861
8	26.893844879592887	18.199592407359123	24.414159255274747	30.492403457773236
9	26.628132200127368	21.131301133271453	24.546552485341174	27.694014181260002
10	30.224080523824746	16.046665736705034	22.97756500305512	30.7516887364151
11	30.875240781942427	17.803984483300127	22.651648066966217	28.66912666779123
12	29.65030168086355	17.461648204495756	23.477442770170462	29.41060734447023
13	30.215463877305687	16.415497501874192	24.39358595918853	28.97545266163159
14	26.90813111111797	20.491704583185864	20.29503734488282	32.30512696081335
15	26.654824157259693	19.944140554064404	22.306505062911345	31.09453022576456
16	29.606404497945615	21.521435943091962	18.9293129052385	29.94284665372392
17	29.805260981944055	18.820720705296377	19.94439315933695	31.42962515342262
18	29.020472534649727	20.222511564409714	18.28306441630921	32.47395148463135
19	29.764198591529134	20.41449157154441	20.872464930669675	28.94884490625678
20	31.85358100251174	17.57299099518338	21.598480551218387	28.974947451086496
21	32.67527788685024	21.976349971694177	18.28121197764387	27.06716016381171
22	31.141037663165466	20.44250268954447	19.842452898238527	28.574006749051538
23	34.03836400475908	21.076513856381553	18.746482822420457	26.138639316438905
24	34.35462580598624	20.73886480874553	19.633997413883353	25.272511971384876
25	32.485136782289246	21.52924582838175	21.267299451808476	24.71831793752052
26	33.84205685834906	19.582777763414015	20.647028589985563	25.92813678825136
27	30.519841206411115	24.85582642769229	18.371126922980313	26.253205442916283
28	31.12651421908402	20.38218714833514	21.22945044689205	27.26184818568879
29	34.930135292280426	20.301996019256013	21.386356904700712	23.38151178376285
30	32.208974206919386	23.69850743065864	18.46965752767231	25.622860834749655
31	29.327401831180218	20.360719000237072	23.397688148824873	26.914191019757837
32	28.865946079601702	19.50207310472945	23.982293374492954	27.649687441175892
33	31.33592520804504	18.2638605861278	23.38149015221883	27.018724053608324
34	29.898200725795267	20.2635594395894	23.66593729164709	26.172302542968247
35	27.195580589749298	18.919068288262473	22.819722867750794	31.06562825423744
36	28.03546126728211	19.739505002683895	26.039344452557923	26.185689277476076
37	28.27426225444932	21.16094687065639	22.89550548692178	27.669285387972515
38	29.878213031995326	20.890508660937197	20.208414706267686	29.02286360079979
39	28.885014255194946	22.252089760749925	20.17281824664953	28.690077737405602
40	28.113837817443475	22.455767039742643	21.581315918156612	27.84907922465727
41	28.3808174635383	19.970953390148953	21.21003742616224	30.438191720150503
42	28.415323657311525	21.530705577799647	20.73632535470786	29.31764541018097
43	28.674981444131088	18.915825589862372	24.380764831183967	28.02842813482258
44	28.513678880646953	20.30452161230157	22.672823461136133	28.50897604591534
45	30.129010348261275	18.87171178579319	26.001084073645806	24.998193792299727
46	29.33063049800868	22.31613624518085	21.473973605579673	26.879259651230797
47	30.40706175458643	18.98272445293003	21.030669049197826	29.57954474328572
48	27.61350533383488	18.399210312162715	21.101346550524532	32.88593780347787
49	29.621835126538592	19.400048645212063	19.328677291607384	31.64943893664196
50	26.09970123956082	27.170572364927565	22.167273663489542	24.562452732022077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30423.0
1	24883.0
2	21684.5
3	20319.0
4	14156.5
5	9821.5
6	6027.0
7	4569.0
8	5271.5
9	5764.5
10	5826.0
11	5477.5
12	5019.5
13	4191.5
14	3249.0
15	3163.5
16	3856.0
17	4320.0
18	4578.5
19	4254.5
20	3743.5
21	3810.5
22	4140.5
23	5736.5
24	7610.5
25	7836.0
26	8912.5
27	11349.0
28	13112.0
29	14638.0
30	15654.5
31	17606.0
32	20819.0
33	22401.0
34	24813.5
35	28737.0
36	31380.0
37	32692.0
38	36540.0
39	45281.0
40	59672.0
41	76273.0
42	87532.0
43	111371.5
44	130921.0
45	116526.5
46	104226.5
47	118038.5
48	144246.0
49	166569.0
50	187468.0
51	202316.5
52	210992.0
53	222812.5
54	237166.0
55	246071.0
56	251518.0
57	263670.0
58	274070.5
59	278537.0
60	284567.0
61	291811.0
62	291856.0
63	283958.5
64	271671.5
65	260253.0
66	238749.0
67	216269.5
68	200507.5
69	183363.0
70	167881.0
71	163482.5
72	163951.0
73	146520.0
74	124644.0
75	113724.5
76	98247.0
77	73254.5
78	55381.0
79	47306.0
80	40947.0
81	33274.0
82	26402.5
83	21661.0
84	18282.0
85	14842.0
86	11049.0
87	8082.0
88	4589.0
89	2125.5
90	1607.0
91	1414.0
92	907.0
93	488.5
94	240.0
95	39.5
96	17.0
97	16.0
98	10.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	23597.0
25	19615.0
26	16243.0
27	14654.0
28	18054.0
29	22225.0
30	19110.0
31	24094.0
32	29087.0
33	24007.0
34	22872.0
35	20689.0
36	16432.0
37	25340.0
38	20667.0
39	23249.0
40	29448.0
41	25106.0
42	41339.0
43	65060.0
44	196864.0
45	351709.0
46	388499.0
47	497939.0
48	562119.0
49	691979.0
50	372874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.182443005706697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.52029467302374	8.7129191360546
2	10.88321836994318	2.6516837502098585
3	4.186600326890407	1.530090596100462
4	2.1458836091567672	1.0456841906173
5	1.2772127019696617	0.7779785473955028
6	0.9218739246316588	0.67384059271634
7	0.6872424584710487	0.5860604456997711
8	0.5449667819932754	0.5311221409309173
9	0.43274979751462894	0.4744754769557772
>10	3.7985509851141788	10.070440615407616
>50	1.622808014885576	14.630762555027923
>100	1.8554700330881893	38.85189604504637
>500	0.07962557186164504	6.57910175808965
>1k	0.039230159795249506	8.715731749780987
>5k	0.0031073393897227334	2.5242177195104394
>10k+	0.001165252271146025	1.643994680456485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	13247	0.3718068939346948	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	11339	0.31825457615501657	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	10157	0.2850790836940209	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	9276	0.2603518342370521	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	8032	0.22543617212074196	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6920	0.1942253873351014	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	6603	0.18532806829099344	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	6267	0.17589747144928908	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5907	0.165793260547463	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	5262	0.14768988268169125	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	5078	0.1425255082207579	No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAG	4504	0.12641490528284635	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	4391	0.12324330574977314	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT	4149	0.11645103064354562	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4064	0.11406531418061444	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	3927	0.11022010058741953	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	3910	0.10974295729483328	No Hit
CCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCCCTTGAG	3807	0.10685203028681083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.010777824961947822	0.0	0.0	0.0	0.0
2	0.019141866208459413	0.0	0.0	0.0	0.0
3	0.02641128460727318	0.0	0.0	0.0	0.0
4	0.033428097733541294	0.0	0.0	0.0	0.0
5	0.04008003657724347	0.0	0.0	0.0	0.0
6	0.052906770972061576	0.0	0.0	0.0	0.0
7	0.06096207244101737	0.0	0.0	0.0	0.0
8	0.07039266928272171	0.0	0.0	0.0	0.0
9	0.07982326612442606	0.0	0.0	0.0	0.0
10	0.08810310561342244	0.0	0.0	0.0	0.0
11	0.09562512928478185	0.0	0.0	0.0	0.0
12	0.10334362372367678	0.0	0.0	0.0	0.0
13	0.11002362981988402	0.0	0.0	0.0	0.0
14	0.11844380557140576	0.0	0.0	0.0	0.0
15	0.12689204857543257	0.0	0.0	0.0	0.0
16	0.13472281202434777	0.0	0.0	0.0	0.0
17	0.144490215896113	0.0	0.0	0.0	0.0
18	0.16225678673182387	0.0	0.0	0.0	0.0
19	0.17275393916872095	0.0	0.0	0.0	0.0
20	0.18302655358557748	0.0	0.0	0.0	0.0
21	0.19346757151746444	0.0	0.0	0.0	0.0
22	0.2020561507840166	0.0	0.0	0.0	0.0
23	0.21333918629105572	0.0	0.0	0.0	0.0
24	0.22403280949548832	0.0	0.0	0.0	0.0
25	0.23573685379010353	0.0	0.0	0.0	0.0
26	0.24598140095445498	0.0	0.0	0.0	0.0
27	0.2584713283192122	0.0	0.0	0.0	0.0
28	0.2701192381088173	0.0	0.0	0.0	0.0
29	0.28283370349361514	0.0	0.0	0.0	0.0
30	0.29574463964594844	0.0	0.0	0.0	0.0
31	0.3077574237181195	0.0	0.0	0.0	0.0
32	0.32179104997065566	0.0	0.0	0.0	0.0
33	0.3333828252552506	0.0	0.0	0.0	0.0
34	0.3447219952672999	0.0	0.0	0.0	0.0
35	0.3544893991390651	0.0	0.0	0.0	0.0
36	0.36408839949579985	0.0	0.0	0.0	0.0
37	0.3709087418545325	0.0	0.0	0.0	0.0
38	0.37481008995273757	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAT	15	2.7036149E-6	377.49442	44
GATATTG	20	1.805347E-8	377.4944	44
GATAGTA	50	0.0	339.74496	44
TAAGCCA	3240	0.0	286.03357	44
CTATCAT	1975	0.0	269.50232	44
GTTCTAA	50	2.910383E-11	226.49664	44
CCGATTG	130	0.0	203.26622	44
TATTGCG	65	0.0	203.26622	44
ATAACTA	65	0.0	203.26622	44
AAGTCGA	65	0.0	203.26622	44
GATTAAC	20	0.0032217682	188.7472	44
TAACTAT	70	1.8189894E-12	188.7472	44
TATGTTA	95	0.0	178.81313	44
CGAATCC	100	0.0	169.87248	44
ACTTCTA	545	0.0	159.30957	44
CCAGAAT	510	0.0	159.13979	44
CGACGGG	1445	0.0	138.45815	44
TACGATG	10	0.009294534	132.44983	43
TCAAGTA	35	2.3646862E-11	132.44983	43
ATAATCT	115	0.0	131.3024	44
>>END_MODULE