FastQCFastQC Report
Tue 24 May 2016
ERR840921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801890
Sequences flagged as poor quality0
Sequence length24-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC89140.4947027842987086No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81470.45213636792479006No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA54230.30096176792146023No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC53970.2995188385528529No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA45240.25106971013768875No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44350.2461304519143788No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC42090.23358806586417594No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40890.2269283918552187No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA36610.20317555455660447No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT34280.19024468752254578No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33050.1834185216633646No Hit
AATTCGTGGAGAAAGAAATGGCTCC32390.17975570095843807No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT29430.16332850506967683No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA26480.14695680646432357No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA25960.14407094772710877No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC24100.133748453013225No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT23920.13274950191188142No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAA23760.13186154537735378No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT23660.13130657254327402No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23380.12975264860785066No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23120.12830971923924325No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21470.119152667476927No Hit
AATTCGTGGAGAAAGAAATGGCTC20100.11154953965003413No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG19840.11010661028142672No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC19430.10783122166169966No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA19260.10688776784376405No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC19130.10616630315946035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18880.10477887107426091No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18300.10156002863659823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG100.0017342326232.137644
GACAATA252.224624E-9232.137644
CGGATAG151.8828014E-5232.137644
CATACCG151.8828014E-5232.137644
CCGACAC202.041179E-7232.137644
GTTCGAA1400.0207.2657244
AGTAGAT500.0185.7100844
TAAGCCA13550.0178.1720444
AACTAGC205.930134E-5174.1032144
AATTTGT800.0159.594644
CTATCAT24950.0150.2614344
TAACAGG750.0139.2825644
TAGAACA353.3143551E-6132.6500544
TTCGCAT353.3143551E-6132.6500544
TTGCCGA1750.0126.0175644
TATACGC204.3759683E-6125.4617243
TATCGCG650.0125.4617243
CGAGCAA152.188365E-4125.46171643
ATACGGA152.188365E-4125.46171643
AGGTCGA301.7807906E-9125.46171643
Produced by FastQC (version 0.11.5)