##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840921.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1801890
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2640161164111	39.0	37.0	39.0	31.0	39.0
2	37.137187619666015	39.0	37.0	39.0	33.0	39.0
3	37.3652814544728	39.0	37.0	39.0	33.0	39.0
4	37.90225096981503	39.0	37.0	40.0	34.0	41.0
5	38.55803073439555	40.0	38.0	41.0	34.0	41.0
6	38.50669463729751	40.0	38.0	41.0	34.0	41.0
7	38.46612667809911	40.0	38.0	41.0	35.0	41.0
8	38.33377509170926	40.0	37.0	41.0	34.0	41.0
9	38.209856872506094	40.0	37.0	41.0	34.0	41.0
10	38.15064793078379	40.0	37.0	41.0	34.0	41.0
11	38.01301078312217	40.0	37.0	41.0	33.0	41.0
12	38.08922242756217	39.0	37.0	41.0	34.0	41.0
13	37.94585185555167	39.0	36.0	40.0	33.0	41.0
14	38.044804066840925	39.0	37.0	40.0	34.0	41.0
15	37.96683149359839	39.0	36.0	40.0	34.0	41.0
16	37.953800731454194	39.0	36.0	40.0	34.0	41.0
17	38.05617601518406	39.0	36.0	41.0	34.0	41.0
18	38.083690458352066	40.0	36.0	41.0	34.0	41.0
19	38.16823557486861	40.0	36.0	41.0	34.0	41.0
20	38.160410457908085	40.0	36.0	41.0	34.0	41.0
21	38.11484718823014	40.0	36.0	41.0	34.0	41.0
22	38.1153855118792	40.0	36.0	41.0	34.0	41.0
23	37.969822797174075	40.0	35.0	41.0	33.0	41.0
24	37.761604759447025	39.0	35.0	41.0	33.0	41.0
25	37.71446311535447	39.0	35.0	41.0	33.0	41.0
26	37.640022914999534	39.0	35.0	41.0	33.0	41.0
27	37.57464971761956	39.0	35.0	41.0	33.0	41.0
28	37.52976367970583	39.0	35.0	41.0	33.0	41.0
29	37.403834135678636	39.0	35.0	41.0	33.0	41.0
30	37.29108943893062	39.0	35.0	41.0	33.0	41.0
31	37.21022079570536	39.0	35.0	41.0	33.0	41.0
32	37.13464146822458	38.0	35.0	41.0	33.0	41.0
33	37.012705281456995	38.0	35.0	40.0	33.0	41.0
34	36.95629581996596	38.0	35.0	40.0	33.0	41.0
35	36.85678417550766	38.0	35.0	40.0	33.0	41.0
36	36.772837866938886	37.0	35.0	40.0	33.0	41.0
37	36.65529468554533	37.0	35.0	40.0	33.0	41.0
38	36.608635191356264	37.0	35.0	40.0	33.0	41.0
39	36.562448265317556	37.0	35.0	40.0	33.0	41.0
40	36.455711346797735	36.0	35.0	40.0	33.0	41.0
41	36.338449239620594	36.0	35.0	40.0	32.0	41.0
42	36.29570180877328	36.0	35.0	40.0	32.0	41.0
43	36.21617384846807	36.0	35.0	40.0	32.0	41.0
44	36.05364265633429	36.0	35.0	40.0	32.0	41.0
45	35.803929012707854	35.0	34.0	39.0	31.0	41.0
46	35.616541341831045	35.0	34.0	39.0	31.0	41.0
47	35.49741301801694	35.0	34.0	39.0	31.0	41.0
48	35.404398482709595	35.0	34.0	39.0	30.0	41.0
49	35.547591963849115	35.0	34.0	39.0	31.0	41.0
50	35.460673150181506	36.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	27.0
22	93.0
23	175.0
24	349.0
25	786.0
26	1308.0
27	2514.0
28	5090.0
29	9313.0
30	16931.0
31	31900.0
32	53174.0
33	93832.0
34	164298.0
35	126923.0
36	189330.0
37	242334.0
38	279574.0
39	336563.0
40	247367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.61991575512379	21.103507983284217	36.276576261592
2	27.36532196748969	22.241923757832055	30.412233821154455	19.980520453523802
3	25.06923286105145	21.367453063172558	26.214419304175063	27.34889477160093
4	21.254016615886652	24.850906548124467	28.25472143138593	25.640355404602943
5	20.144736915128004	23.11128870241802	29.33708494969171	27.406889432762267
6	23.351147961307294	28.14067451398254	23.544777983117726	24.96339954159244
7	29.532657376421422	22.436164249759972	20.31683399097614	27.71434438284246
8	23.409475606169078	26.015406045874055	24.963177552458806	25.61194079549806
9	24.610714305534746	28.297454339610077	24.965286449228312	22.12654490562687
10	28.07074793688849	20.498809583270898	22.185261031472507	29.2451814483681
11	25.95330458574053	25.213359306061967	23.498770735172513	25.334565373024994
12	25.57769897163534	23.223060231201682	26.171242417683654	25.027998379479328
13	23.219619399630385	26.387903812108398	23.592339154998363	26.80013763326285
14	23.134986042433223	25.735533245647623	21.433328338577827	29.696152373341324
15	22.745117626492185	26.310207615337227	24.00423999245237	26.94043476571822
16	23.569529771517683	28.45212526846811	20.602534005960408	27.375810954053797
17	27.273584958016308	28.91031084028437	18.082624355537796	25.73347984616153
18	25.536131506362764	27.07396122959781	19.44336224741799	27.94654501662144
19	25.162079816192996	25.900082690952274	22.22527457280966	26.712562920045062
20	26.46759791108225	23.088368324370524	23.964004461981585	26.48002930256564
21	24.4865668825511	31.215057522934252	20.052888911087802	24.245486683426844
22	25.877162312904783	28.484924162962223	20.26067074016727	25.377242783965727
23	27.953704166181065	27.428200389590927	20.90016593687739	23.717929507350615
24	26.38834779037566	27.854086542463747	21.665251485939763	24.09231418122083
25	24.89345951573422	27.412802302683442	21.2886394464256	26.40509873515674
26	26.109481024782188	29.434632037555385	22.675720117366215	21.780166820296206
27	24.3942547864998	32.237797867966854	18.234871991490415	25.133075354042926
28	24.957926729178773	26.483526532429785	22.016120466722217	26.54242627166922
29	27.5686797115552	30.009502583960895	20.468508779189058	21.953308925294852
30	25.951944462004402	30.127810950134187	21.130931514249426	22.78931307361198
31	24.0214801408602	29.291263918837917	22.001494531792297	24.68576140850959
32	23.474805914489107	29.03525051770658	22.0659469601257	25.42399660767861
33	22.347046450523045	29.103230923652497	23.340375899916005	25.209346725908453
34	24.237890526868203	30.170268920288777	21.96604976222596	23.62579079061706
35	23.014417986598165	27.896920988864967	22.44854695024172	26.640114074295145
36	22.080699487567536	28.40514169525081	25.79205735626727	23.722101460914384
37	21.11390185064114	32.11360935136341	20.07107313738075	26.701415660614703
38	24.768063042083817	30.347427061257353	19.370000373072806	25.514509523586028
39	23.044345743601856	31.088282756720588	22.24904705244769	23.618324447229867
40	21.952695645383287	32.93947609890378	19.89032925626934	25.217498999443595
41	24.35850029168197	29.987869733905793	19.921045240028025	25.73258473438421
42	23.689203872788312	30.95242847679558	18.952635564432324	26.405732085983786
43	22.202924593936643	26.812854121335544	23.045909205538962	27.938312079188847
44	21.80517085771194	27.13468401178472	21.58492053612183	29.475224594381512
45	23.21451498229459	26.87390851171042	27.27336734059957	22.63820916539542
46	22.61217008121787	32.002928514140145	22.099526459570836	23.285374945071155
47	25.301125070558232	27.292292191065258	23.079942707634263	24.326640030742244
48	23.432449069521546	27.449815160121677	20.48229921298689	28.635436557369882
49	21.789025028871464	28.63320929296993	20.498565963925408	29.0791997142332
50	20.042768149253156	35.34966803464988	24.324384887733608	20.28317892836335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47367.0
1	37598.0
2	28053.0
3	23605.0
4	15261.5
5	10433.0
6	6704.5
7	4223.0
8	4280.0
9	3855.5
10	3118.5
11	3834.0
12	5331.0
13	5545.0
14	5268.0
15	5461.0
16	5641.5
17	5441.0
18	5358.0
19	5973.5
20	7162.0
21	9368.0
22	12635.0
23	16995.5
24	21712.0
25	24175.5
26	24799.0
27	24620.5
28	23057.0
29	21835.5
30	22472.5
31	25779.0
32	32779.0
33	39438.0
34	47701.5
35	55756.0
36	56166.0
37	51633.0
38	49306.5
39	55077.5
40	65779.5
41	73109.5
42	74695.0
43	83675.5
44	95255.5
45	89783.5
46	80950.5
47	75829.0
48	79840.0
49	97626.0
50	111628.5
51	115416.5
52	110028.5
53	103627.5
54	101832.0
55	98563.5
56	95295.0
57	94610.0
58	96372.0
59	99092.0
60	98158.5
61	94799.5
62	91927.5
63	88747.0
64	82881.0
65	77584.5
66	75866.5
67	73804.0
68	68513.5
69	63434.0
70	58228.0
71	55877.0
72	55761.0
73	50022.0
74	41541.0
75	35038.5
76	27843.0
77	20398.0
78	17366.5
79	17471.0
80	16306.0
81	11918.5
82	8124.0
83	7235.0
84	6601.5
85	5040.0
86	3597.0
87	2369.5
88	1177.5
89	566.0
90	335.5
91	179.0
92	118.5
93	98.0
94	49.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	13368.0
25	11520.0
26	8643.0
27	7740.0
28	8464.0
29	12085.0
30	8424.0
31	10111.0
32	11931.0
33	9881.0
34	10999.0
35	7722.0
36	9203.0
37	9925.0
38	10419.0
39	12367.0
40	19173.0
41	16729.0
42	24984.0
43	35866.0
44	93626.0
45	147026.0
46	213930.0
47	195901.0
48	315159.0
49	264312.0
50	312382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.353390984507975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11705913541469	11.225947965434123
2	10.71326770291489	3.289699755291079
3	4.188026936585211	1.9290124503313177
4	2.116768011677813	1.2999826762715603
5	1.3303584456954891	1.0212756683152582
6	0.867011837573922	0.7986943038281489
7	0.6213125140385583	0.6677477766121108
8	0.4960547981471087	0.6092898612554993
9	0.39102082002772426	0.5403145979671719
>10	3.290932550529187	11.296049877893484
>50	1.4462962031538211	16.29703667360522
>100	1.3440269658074682	34.4552714459051
>500	0.04590494176341979	4.833833543450982
>1k	0.029634835821954548	9.248446980211991
>5k	0.002324300848780749	2.4873964236269765
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8914	0.4947027842987086	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8147	0.45213636792479006	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	5423	0.30096176792146023	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	5397	0.2995188385528529	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4524	0.25106971013768875	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4435	0.2461304519143788	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	4209	0.23358806586417594	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4089	0.2269283918552187	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	3661	0.20317555455660447	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	3428	0.19024468752254578	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3305	0.1834185216633646	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	3239	0.17975570095843807	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	2943	0.16332850506967683	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2648	0.14695680646432357	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	2596	0.14407094772710877	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	2410	0.133748453013225	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	2392	0.13274950191188142	No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAAAAAAAA	2376	0.13186154537735378	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	2366	0.13130657254327402	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2338	0.12975264860785066	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2312	0.12830971923924325	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2147	0.119152667476927	No Hit
AATTCGTGGAGAAAGAAATGGCTC	2010	0.11154953965003413	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG	1984	0.11010661028142672	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	1943	0.10783122166169966	No Hit
AGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	1926	0.10688776784376405	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	1913	0.10616630315946035	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1888	0.10477887107426091	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1830	0.10156002863659823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025084772100405685	0.0	0.0	0.0	0.0
2	0.04356536747526209	0.0	0.0	0.0	0.0
3	0.0539988567559618	0.0	0.0	0.0	0.0
4	0.06964909067701136	0.0	0.0	0.0	0.0
5	0.07836216417206378	0.0	0.0	0.0	0.0
6	0.1089411673298592	0.0	0.0	0.0	0.0
7	0.12026261314508654	0.0	0.0	0.0	0.0
8	0.1320835345109857	0.0	0.0	0.0	0.0
9	0.14418194229392473	0.0	0.0	0.0	0.0
10	0.1544489397244005	0.0	0.0	0.0	0.0
11	0.16421646160420447	0.0	0.0	0.0	0.0
12	0.17598188568669565	0.0	0.0	0.0	0.0
13	0.19007819567232184	0.0	0.0	0.0	0.0
14	0.20117765235391727	0.0	0.0	0.0	0.0
15	0.21288757915300047	0.0	0.0	0.0	0.0
16	0.2234875602839241	0.0	0.0	0.0	0.0
17	0.23802784853681414	0.0	0.0	0.0	0.0
18	0.26355659890448363	0.0	0.0	0.0	0.0
19	0.27798589259055767	0.0	0.0	0.0	0.0
20	0.28997330580668074	0.0	0.0	0.0	0.0
21	0.3030706646909634	0.0	0.0	0.0	0.0
22	0.3172779692434055	0.0	0.0	0.0	0.0
23	0.33181825749629557	0.0	0.0	0.0	0.0
24	0.34469362724694624	0.0	0.0	0.0	0.0
25	0.3606768448684437	0.0	0.0	0.0	0.0
26	0.3751616358379257	0.0	0.0	0.0	0.0
27	0.3873155409042727	0.0	0.0	0.0	0.0
28	0.4014673481733069	0.0	0.0	0.0	0.0
29	0.4177835494952522	0.0	0.0	0.0	0.0
30	0.43276781601540604	0.0	0.0	0.0	0.0
31	0.44830705536963966	0.0	0.0	0.0	0.0
32	0.46190388980459407	0.0	0.0	0.0	0.0
33	0.4749457514054687	0.0	0.0	0.0	0.0
34	0.48626719722069606	0.0	0.0	0.0	0.0
35	0.49670068650139576	0.0	0.0	0.0	0.0
36	0.5056912464134881	0.0	0.0	0.0	0.0
37	0.5125729095560773	0.0	0.0	0.0	0.0
38	0.5187886052977707	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	10	0.0017342326	232.1376	44
GACAATA	25	2.224624E-9	232.1376	44
CGGATAG	15	1.8828014E-5	232.1376	44
CATACCG	15	1.8828014E-5	232.1376	44
CCGACAC	20	2.041179E-7	232.1376	44
GTTCGAA	140	0.0	207.26572	44
AGTAGAT	50	0.0	185.71008	44
TAAGCCA	1355	0.0	178.17204	44
AACTAGC	20	5.930134E-5	174.10321	44
AATTTGT	80	0.0	159.5946	44
CTATCAT	2495	0.0	150.26143	44
TAACAGG	75	0.0	139.28256	44
TAGAACA	35	3.3143551E-6	132.65005	44
TTCGCAT	35	3.3143551E-6	132.65005	44
TTGCCGA	175	0.0	126.01756	44
TATACGC	20	4.3759683E-6	125.46172	43
TATCGCG	65	0.0	125.46172	43
CGAGCAA	15	2.188365E-4	125.461716	43
ATACGGA	15	2.188365E-4	125.461716	43
AGGTCGA	30	1.7807906E-9	125.461716	43
>>END_MODULE