FastQCFastQC Report
Tue 24 May 2016
ERR840920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6563855
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA194060.2956494316221184No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG83290.1268918950829962No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69980.10661417718703413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACC400.0241.5037244
CTATCAT80250.0206.1863644
TCGTACA950.0188.8450344
ACATTGG15000.0149.9623344
ACTTCTA23550.0124.81721544
ATTACGA302.5632653E-7104.88251543
CCCATAC18000.0101.9682444
ACCACTC9600.0100.6265644
CCTATCA86050.087.9038643
CATGACC9950.085.9912844
AATCTAC1450.085.65649444
TTAATCG203.12207E-584.4851242
GGATAAT9250.082.99892443
ACCCATA500.001142427982.8012844
CATACAA4250.077.9306144
TCCGCAC4200.076.41440643
CCCTATC104350.074.40520542
AATTGCC5000.074.2568243
TTACGAG952.2812092E-672.63270644
CCAGAAT6500.072.1857344
Produced by FastQC (version 0.11.5)