##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840920.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6563855
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.57883286574734	39.0	37.0	39.0	32.0	39.0
2	37.40709125963325	39.0	37.0	39.0	35.0	39.0
3	37.44122211718571	39.0	37.0	39.0	34.0	39.0
4	37.98633059383548	39.0	37.0	40.0	34.0	41.0
5	38.468835768005235	40.0	38.0	41.0	34.0	41.0
6	38.40018205764753	40.0	38.0	41.0	34.0	41.0
7	38.297347823801715	40.0	38.0	41.0	34.0	41.0
8	38.19683600567045	39.0	37.0	41.0	34.0	41.0
9	37.97738630728436	39.0	37.0	40.0	33.0	41.0
10	37.89202930290203	39.0	37.0	40.0	33.0	41.0
11	37.71442452644064	39.0	36.0	40.0	33.0	41.0
12	37.66029505526859	39.0	36.0	40.0	33.0	41.0
13	37.43683445170559	39.0	36.0	40.0	33.0	41.0
14	37.32007943502713	38.0	36.0	40.0	32.0	41.0
15	37.285335980151906	38.0	35.0	40.0	32.0	41.0
16	37.27245269738591	38.0	35.0	40.0	32.0	41.0
17	37.332950834532454	38.0	35.0	40.0	33.0	41.0
18	37.44993056671727	39.0	35.0	40.0	33.0	41.0
19	37.52805889222111	39.0	35.0	40.0	33.0	41.0
20	37.55251509973941	39.0	35.0	40.0	33.0	41.0
21	37.49726022284161	39.0	35.0	40.0	33.0	41.0
22	37.37667148344989	39.0	35.0	40.0	33.0	41.0
23	37.19067895314567	39.0	35.0	40.0	33.0	41.0
24	36.94361636568754	38.0	35.0	40.0	32.0	41.0
25	36.84193725980929	38.0	35.0	40.0	32.0	41.0
26	36.659835749335016	38.0	35.0	40.0	32.0	41.0
27	36.54812975096608	37.0	35.0	40.0	32.0	41.0
28	36.42271237228972	37.0	35.0	40.0	31.0	41.0
29	36.27656849385503	37.0	35.0	40.0	31.0	41.0
30	36.12678589291867	37.0	34.0	40.0	31.0	41.0
31	35.966599100011514	36.0	34.0	40.0	31.0	41.0
32	35.848138066789645	36.0	34.0	40.0	31.0	41.0
33	35.70682110404875	36.0	34.0	39.0	31.0	41.0
34	35.620408980465534	35.0	34.0	39.0	31.0	41.0
35	35.383097443540734	35.0	34.0	39.0	30.0	41.0
36	35.375277904940255	35.0	34.0	39.0	31.0	41.0
37	35.28954350856485	35.0	34.0	39.0	31.0	40.0
38	35.30973468332079	35.0	34.0	39.0	31.0	41.0
39	35.223801752695934	35.0	34.0	39.0	31.0	40.0
40	35.07573227645647	35.0	34.0	38.0	30.0	40.0
41	35.01020213273081	35.0	34.0	38.0	31.0	40.0
42	34.94254445265922	35.0	34.0	38.0	31.0	40.0
43	34.813716105210005	35.0	33.0	37.0	30.0	40.0
44	34.65538420797715	35.0	33.0	37.0	30.0	40.0
45	34.403521800599925	35.0	33.0	37.0	30.0	39.0
46	34.129652977873036	35.0	33.0	37.0	29.0	39.0
47	33.95825382634686	35.0	33.0	36.0	29.0	39.0
48	33.99793869658891	35.0	33.0	36.0	29.0	39.0
49	33.92160422119546	35.0	33.0	36.0	29.0	39.0
50	34.11045168741178	35.0	33.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	16.0
21	49.0
22	147.0
23	405.0
24	950.0
25	2576.0
26	5565.0
27	12209.0
28	26137.0
29	51318.0
30	94657.0
31	165436.0
32	269208.0
33	428546.0
34	649412.0
35	741575.0
36	1001026.0
37	1061953.0
38	926235.0
39	829912.0
40	296517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.28427958874777	17.939381659101244	35.776338752150984
2	27.188199617450415	18.772169708197392	29.77073686118904	24.268893813163146
3	25.14827033808638	18.08048776214587	29.626797057521838	27.144444842245907
4	22.294779516000887	19.786025133096327	30.282356938110304	27.636838412792482
5	22.724085160321184	19.527488038660206	28.449501093488504	29.298925707530106
6	25.171107527512415	21.880114658230568	25.18142158838061	27.767356225876412
7	28.60939188936989	18.96781693075182	21.19589783747508	31.226893342403205
8	27.28609940347555	19.65434946384404	23.972528338910596	29.08702279376982
9	28.109776952720615	19.320719302909648	24.771251040737493	27.798252703632244
10	30.57730556205157	17.876415612471636	22.793008072238035	28.75327075323876
11	29.563252082808045	17.57407803798225	22.324975186075864	30.537694693133837
12	30.21795880621982	20.709887710804093	21.737850699017574	27.334302783958513
13	30.133237251584628	19.19283713610371	22.65289224091635	28.021033371395315
14	27.699773989522924	20.428437252194023	20.648079520342847	31.223709237940206
15	27.497895672588747	22.269367620095203	20.935456374340994	29.297280332975056
16	27.83414624485154	21.52724275597191	20.979470143688427	29.659140855488126
17	28.152130112563423	22.531500162633087	19.150910554849247	30.16545916995424
18	28.059593028791767	21.263754302921072	18.18128218859192	32.49537047969524
19	30.401859882645184	18.815162735922716	22.391323391513065	28.391653989919035
20	29.45410890399011	18.084448849037646	24.037596808582762	28.423845438389485
21	30.862153414418813	24.34627212209898	17.91465533592683	26.876919127555375
22	32.102796298821346	21.26989398760332	19.14204381419151	27.48526589938382
23	33.103244968086585	20.693830073942827	21.427271016803388	24.775653941167196
24	30.890901764283335	21.768747176773406	21.779015532792847	25.56133552615041
25	30.98299917495545	21.327094892391493	21.456694362400054	26.233211570253
26	30.878467594630248	22.428629542773507	21.70253271216688	24.99037015042936
27	31.263034773933047	22.836000565502985	19.648202518350523	26.25276214221345
28	31.280488967326235	21.038117987088256	21.145439777130708	26.535953268454797
29	31.370680251143586	23.638467003034492	20.922054559745185	24.06879818607674
30	30.715135213240114	23.402394157987754	20.204753065738515	25.677717563033614
31	29.311913758922664	21.76360979191829	21.840484942734335	27.083991506424702
32	28.533152644369412	22.410100616481536	22.9141039174802	26.142642821668844
33	27.71959748627197	20.108469756883025	24.06064436597136	28.111288390873646
34	30.211674868851553	19.931449343784013	22.89001986485963	26.966855922504802
35	26.67938090053704	20.79354451825502	22.810766122693742	29.716308458514195
36	26.352426025508606	20.678037785018034	25.51713610287627	27.45240008659709
37	25.235949573655848	24.387884260613724	23.62544546881322	26.750720696917206
38	26.69016822015438	21.110689018025944	23.093634719132574	29.1055080426871
39	28.33403366422897	21.27346387089773	23.308349435800743	27.08415302907256
40	26.567585613353607	21.394598904649758	24.809174690694434	27.228640791302205
41	27.877073466483804	21.698967053901537	20.962486480094146	29.46147299952051
42	26.521317397806026	22.907194316933502	21.892962176569675	28.678526108690793
43	27.741026298220824	21.062548503302462	21.656404611940584	29.540020586536137
44	25.691296812527636	22.59887406398589	21.70170461830922	30.008124505177257
45	28.180338167294387	19.203576356761854	26.64689005796785	25.96919541797591
46	26.343894717849743	22.445043439275228	23.09635372528007	28.114708117594954
47	25.339494182142904	22.136955367391188	25.00820423687075	27.51534621359516
48	25.687503014077862	20.3210432815064	21.248662401121955	32.742791303293785
49	25.826241558045805	23.11181179694862	20.65785859926884	30.40408804573673
50	24.833932161341373	22.727233842337142	24.52970614654177	27.90912784977972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46598.0
1	43123.5
2	48775.5
3	50417.5
4	38283.0
5	28996.0
6	20313.0
7	16319.5
8	16539.5
9	16179.0
10	14807.5
11	12795.5
12	10976.5
13	9678.5
14	8914.0
15	8609.5
16	8338.5
17	8977.5
18	10045.0
19	10611.5
20	11110.0
21	11512.5
22	11705.5
23	12841.5
24	14651.0
25	16661.5
26	20420.5
27	24725.0
28	26750.0
29	26704.0
30	29406.5
31	42874.5
32	64228.5
33	72639.5
34	75339.0
35	85469.0
36	91236.5
37	94663.5
38	101716.5
39	121248.0
40	145754.0
41	166818.0
42	191053.0
43	208875.0
44	215505.0
45	227792.5
46	248500.0
47	256614.5
48	280456.5
49	345198.5
50	415939.5
51	454664.5
52	457233.0
53	439369.5
54	424669.5
55	421934.5
56	432904.0
57	454970.5
58	465038.0
59	466246.0
60	475226.0
61	477745.5
62	461083.0
63	441695.0
64	430600.5
65	427942.5
66	409617.0
67	378868.5
68	352582.5
69	328348.5
70	302650.0
71	277779.5
72	263791.0
73	253418.0
74	237418.5
75	217693.0
76	190590.0
77	147159.5
78	114928.0
79	100538.0
80	82157.0
81	62885.5
82	48683.5
83	38501.0
84	30496.5
85	23394.0
86	16356.0
87	11367.5
88	7373.0
89	4538.0
90	2753.5
91	1313.0
92	902.5
93	792.0
94	595.5
95	389.5
96	370.5
97	367.0
98	191.0
99	7.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	42995.0
25	41008.0
26	36031.0
27	44375.0
28	49453.0
29	52163.0
30	52639.0
31	55549.0
32	61851.0
33	59286.0
34	56870.0
35	43767.0
36	40966.0
37	46156.0
38	47789.0
39	48871.0
40	50873.0
41	53744.0
42	72892.0
43	108396.0
44	307200.0
45	618506.0
46	726901.0
47	776642.0
48	1008094.0
49	1125678.0
50	935160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.904837430378393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71293161207143	13.735325693218744
2	10.140629863147517	3.631326582825932
3	3.3710299979908904	1.8107323226086707
4	1.606478124751897	1.1505491863656743
5	0.9162196076029462	0.8202381562327917
6	0.6291507855200319	0.6758905520358621
7	0.46424940885741556	0.5818617134918906
8	0.33452701820012976	0.4791721501554046
9	0.2996642920481362	0.482889638953018
>10	3.0550887562997464	13.097562427747327
>50	1.384472792012352	17.97629571151337
>100	1.019856860409472	31.257094729649754
>500	0.04056531673235127	4.913055818617494
>1k	0.024140096460356274	7.595075696564753
>5k	7.466009214543177E-4	0.9282132593041929
>10k+	2.4886697381810595E-4	0.8647163607151441
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	19406	0.2956494316221184	No Hit
CTCTTTCCCTGCCGCCGCCGAGTCGCGCGGAGGCGGAGGCTTGGG	8329	0.1268918950829962	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6998	0.10661417718703413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.017687776466725726	0.0	0.0	0.0	0.0
2	0.03249614746212401	0.0	0.0	0.0	0.0
3	0.04293208792698803	0.0	0.0	0.0	0.0
4	0.05374890213144562	0.0	0.0	0.0	0.0
5	0.06435242704173082	0.0	0.0	0.0	0.0
6	0.07973972612131133	0.0	0.0	0.0	0.0
7	0.09191245083872206	0.0	0.0	0.0	0.0
8	0.10248550584983977	0.0	0.0	0.0	0.0
9	0.11167218044883685	0.0	0.0	0.0	0.0
10	0.11965529403071823	0.0	0.0	0.0	0.0
11	0.12712041932675233	0.0	0.0	0.0	0.0
12	0.13662702786700803	0.0	0.0	0.0	0.0
13	0.1458137024660051	0.0	0.0	0.0	0.0
14	0.15525937120792582	0.0	0.0	0.0	0.0
15	0.1658933660173785	0.0	0.0	0.0	0.0
16	0.17634454143182626	0.0	0.0	0.0	0.0
17	0.1881668623088109	0.0	0.0	0.0	0.0
18	0.21467567458452388	0.0	0.0	0.0	0.0
19	0.22537060919231153	0.0	0.0	0.0	0.0
20	0.23525809147216079	0.0	0.0	0.0	0.0
21	0.24686712305497302	0.0	0.0	0.0	0.0
22	0.25652608109106617	0.0	0.0	0.0	0.0
23	0.26741907004344245	0.0	0.0	0.0	0.0
24	0.27872340263457984	0.0	0.0	0.0	0.0
25	0.28922028289777885	0.0	0.0	0.0	0.0
26	0.3034344908594111	0.0	0.0	0.0	0.0
27	0.31316962364342293	0.0	0.0	0.0	0.0
28	0.3251595289658288	0.0	0.0	0.0	0.0
29	0.3367990304478085	0.0	0.0	0.0	0.0
30	0.3497639725435739	0.0	0.0	0.0	0.0
31	0.3616929380676447	0.0	0.0	0.0	0.0
32	0.3751301636005061	0.0	0.0	0.0	0.0
33	0.3856727487124563	0.0	0.0	0.0	0.0
34	0.395179357252712	0.0	0.0	0.0	0.0
35	0.40395468821294805	0.0	0.0	0.0	0.0
36	0.41297377836652394	0.0	0.0	0.0	0.0
37	0.4192505775950261	0.0	0.0	0.0	0.0
38	0.4237448877222303	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATACC	40	0.0	241.50372	44
CTATCAT	8025	0.0	206.18636	44
TCGTACA	95	0.0	188.84503	44
ACATTGG	1500	0.0	149.96233	44
ACTTCTA	2355	0.0	124.817215	44
ATTACGA	30	2.5632653E-7	104.882515	43
CCCATAC	1800	0.0	101.96824	44
ACCACTC	960	0.0	100.62656	44
CCTATCA	8605	0.0	87.90386	43
CATGACC	995	0.0	85.99128	44
AATCTAC	145	0.0	85.656494	44
TTAATCG	20	3.12207E-5	84.48512	42
GGATAAT	925	0.0	82.998924	43
ACCCATA	50	0.0011424279	82.80128	44
CATACAA	425	0.0	77.93061	44
TCCGCAC	420	0.0	76.414406	43
CCCTATC	10435	0.0	74.405205	42
AATTGCC	500	0.0	74.25682	43
TTACGAG	95	2.2812092E-6	72.632706	44
CCAGAAT	650	0.0	72.18573	44
>>END_MODULE