FastQCFastQC Report
Tue 24 May 2016
ERR840919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5698918
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC472230.8286309787226278No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA382390.6709870189393846No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220360.38666989067047464No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC211410.3709651551399757No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC183040.32118377558687455No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG156880.2752803251424218No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA147270.2584174750364894No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG125680.22053309066738633No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124130.21781327613417142No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA119110.20900458648466252No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT103310.18128002543640742No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG100110.17566492446460888No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG98930.1735943559812582No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA88260.15487150367841757No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85660.15030923413883127No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG79840.14009676924637274No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT70590.12386561799976768No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT68170.11961919788984504No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC65500.11493409801650066No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCCGTGG57720.10128238377881556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA128700.0223.1981244
CTATCAT49200.0198.5206544
TGTCGTA259.773627E-5153.6128244
AACGCAC3800.0144.8541944
CTATCTA5700.0139.2396944
ACTTCTA13600.0126.12817444
CCGTACC204.304273E-6125.89403543
AGGTACA1950.0124.72835544
CGACGGG58350.0117.3705544
TTAAGCC123850.0115.47488443
TTCATAG2100.0109.72343444
TCTAGAC1200.0106.6755744
GCTATAC302.5588997E-7104.91170543
TTATGAG4600.0102.9651244
TTTGTAC3200.092.00767544
GCATACC2900.088.2832344
TATTATG800.086.55215543
CGAATTT602.7284841E-1183.9293643
CGATCAT303.2256165E-583.9293643
ACATTGG9400.083.0707644
Produced by FastQC (version 0.11.5)