##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840919.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5698918
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.952560994911664	39.0	37.0	39.0	33.0	39.0
2	37.56882850393706	39.0	37.0	39.0	35.0	39.0
3	37.85413283714558	39.0	37.0	39.0	35.0	40.0
4	38.40073291105434	39.0	38.0	40.0	35.0	41.0
5	39.00816190020632	40.0	38.0	41.0	35.0	41.0
6	38.958501245324115	40.0	38.0	41.0	35.0	41.0
7	38.86667083119989	40.0	38.0	41.0	35.0	41.0
8	38.75045894676849	40.0	38.0	41.0	35.0	41.0
9	38.67395372244345	40.0	38.0	41.0	35.0	41.0
10	38.58056704799051	40.0	38.0	41.0	35.0	41.0
11	38.52739695500093	40.0	38.0	41.0	35.0	41.0
12	38.48847307506443	40.0	38.0	41.0	35.0	41.0
13	38.3506035707129	40.0	37.0	41.0	34.0	41.0
14	38.381083742563064	40.0	37.0	41.0	34.0	41.0
15	38.33633595008737	40.0	37.0	41.0	34.0	41.0
16	38.33474266518662	40.0	37.0	41.0	34.0	41.0
17	38.34434396143268	40.0	37.0	41.0	34.0	41.0
18	38.37566762673195	40.0	37.0	41.0	34.0	41.0
19	38.395344870728096	40.0	37.0	41.0	34.0	41.0
20	38.411848880787545	40.0	37.0	41.0	34.0	41.0
21	38.342706457611776	40.0	37.0	41.0	34.0	41.0
22	38.28031145561315	40.0	36.0	41.0	34.0	41.0
23	38.16082368618043	40.0	36.0	41.0	34.0	41.0
24	37.99141486155793	40.0	35.0	41.0	34.0	41.0
25	37.93301987442694	40.0	35.0	41.0	33.0	41.0
26	37.85101557311445	40.0	35.0	41.0	33.0	41.0
27	37.73409207885143	39.0	35.0	41.0	33.0	41.0
28	37.663550431897114	39.0	35.0	41.0	33.0	41.0
29	37.531046404671294	39.0	35.0	41.0	33.0	41.0
30	37.364511615446716	39.0	35.0	41.0	33.0	41.0
31	37.27919869766253	39.0	35.0	41.0	33.0	41.0
32	37.130018419173815	38.0	35.0	41.0	33.0	41.0
33	37.021294235237214	38.0	35.0	41.0	33.0	41.0
34	36.88668408938698	38.0	35.0	40.0	33.0	41.0
35	36.75890503721427	37.0	35.0	40.0	33.0	41.0
36	36.67467850294393	37.0	35.0	40.0	33.0	41.0
37	36.5834820696248	37.0	35.0	40.0	33.0	41.0
38	36.46814848149616	36.0	35.0	40.0	33.0	41.0
39	36.389616035944876	36.0	35.0	40.0	33.0	41.0
40	36.27841318842475	36.0	35.0	40.0	32.0	41.0
41	36.17882000992664	35.0	35.0	40.0	32.0	41.0
42	36.099524195216844	35.0	35.0	40.0	32.0	41.0
43	35.94122890165976	35.0	35.0	40.0	32.0	41.0
44	35.77673860794617	35.0	34.0	39.0	31.0	41.0
45	35.58232438835632	35.0	34.0	39.0	31.0	41.0
46	35.33800542181854	35.0	34.0	39.0	30.0	41.0
47	35.274956210114404	35.0	34.0	39.0	31.0	41.0
48	35.258749094304065	35.0	34.0	39.0	30.0	41.0
49	35.425145034631846	35.0	34.0	39.0	31.0	41.0
50	35.658947815219186	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	7.0
20	22.0
21	69.0
22	176.0
23	386.0
24	914.0
25	1755.0
26	3051.0
27	6192.0
28	12254.0
29	24673.0
30	46151.0
31	84287.0
32	142025.0
33	249047.0
34	479669.0
35	409175.0
36	571975.0
37	802663.0
38	971566.0
39	992972.0
40	899888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	36.387293166878344	23.676617210495046	39.93608962262661
2	24.67973745191631	21.10135643292288	30.406526291481995	23.81237982367881
3	25.163531042208366	17.63078535258798	27.564249915510274	29.641433689693375
4	20.758607160166193	20.34007157148076	29.839295810187128	29.06202545816592
5	23.293614682646776	19.051844578216425	33.00252784826874	24.652012890868054
6	28.869076551022495	23.483194529207125	23.35581947309998	24.291909446670402
7	31.69689404199183	17.644103670205467	21.870677907630885	28.788324380171815
8	26.935183134763474	20.89731770136015	24.716604099234278	27.450895064642094
9	24.854331997758173	24.170868926347072	26.61775445795149	24.357044617943266
10	30.165182232837886	17.47521196128809	22.233571355123903	30.126034450750122
11	28.28443925671505	23.779110350420904	21.865589222375196	26.07086117048885
12	27.246838785888833	20.699999543773046	23.894658600106194	28.158503070231927
13	26.36451340412338	20.228471439666265	23.481545093296656	29.925470062913696
14	24.544325782543282	21.850779393562075	21.519190133986836	32.0857046899078
15	26.45933842178463	20.92233999506573	25.630549518347166	26.987772064802478
16	28.13830625392399	22.81877015952853	18.631694648001602	30.411228938545875
17	27.486006992906376	21.51829522726946	20.19620215626896	30.799495623555206
18	27.986031734445028	22.944565968487353	17.327885749540528	31.741516547527095
19	27.044589867760866	26.311169944891294	19.82632492694227	26.817915260405574
20	32.28632171931584	19.751363328968765	20.482502117068538	27.479812834646854
21	30.06604060630457	23.03309154474586	20.312557576718948	26.588310272230625
22	28.786324000450612	24.930855295689465	20.98949309325033	25.293327610609595
23	33.627997454955484	21.879083011898047	17.189298038680327	27.30362149446614
24	30.427793486412686	23.199719666083983	20.14978983729894	26.222697010204392
25	31.371319745916086	24.747511216672407	20.376641497088777	23.50452754032273
26	30.11648261044792	21.244497471178246	23.74369568425773	24.895324234116103
27	27.700102457902915	28.27793581264474	17.786057708153358	26.23590402129899
28	27.51663813120396	21.235018882077917	24.32064244874353	26.92770053797459
29	35.21433188806243	23.881395935279013	19.072629629216607	21.831642547441948
30	28.823268543625353	26.17607981841022	18.325923968330397	26.674727669634034
31	26.80613677201103	22.508171920050344	26.001768997472833	24.683922310465793
32	25.380647360989624	22.317670046841723	24.88729036722438	27.414392224944272
33	29.467743738633683	21.375757247046558	21.60314812580549	27.553350888514274
34	26.16592459585704	23.388600953579395	26.17230826672387	24.2731661838397
35	25.167526788785704	22.781364888118137	20.467943285351506	31.583165037744653
36	23.88290659767219	22.356058365005023	29.202924557059344	24.558110480263444
37	25.850304606142654	23.8495767944988	21.49078828034701	28.809330319011533
38	25.87957340701617	25.741759581276668	20.190655107051406	28.188011904655752
39	25.585547999180854	26.076388961784907	19.24776780729902	29.09029523173522
40	25.52213239856812	28.785398672407545	20.06547504844082	25.626993880583516
41	28.605307648162796	23.726792610860358	18.464667380143627	29.203232360833216
42	27.89152307981717	25.867866594757544	19.13168560277927	27.108924722646016
43	25.88332987995748	21.104492802291457	24.83991419750293	28.172263120248136
44	27.176388893956034	21.29511734086027	23.563393818814347	27.96509994636935
45	27.56874385811126	23.973037378057356	24.718346679044632	23.73987208478675
46	26.054552400528756	29.207499747648303	19.788720048026512	24.949227803796433
47	32.48802731488442	19.2903178140963	20.680957515833498	27.540697355185777
48	25.264835514163224	20.345904249682828	19.40412367295857	34.98513656319538
49	26.465456390347203	22.70519918824723	19.047679445890502	31.781664975515067
50	23.529808084244852	33.87367539276423	19.40717334402339	23.189343178967533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110719.0
1	80593.0
2	50851.0
3	43689.5
4	28798.5
5	18534.5
6	10949.5
7	6932.0
8	7976.5
9	8873.0
10	9197.0
11	8901.0
12	8889.5
13	8870.5
14	8929.0
15	8599.5
16	7161.0
17	5879.0
18	5707.5
19	6687.5
20	7859.5
21	8707.5
22	9893.0
23	13313.5
24	17606.5
25	20710.0
26	22626.5
27	22796.5
28	22568.5
29	23569.5
30	26651.5
31	37993.0
32	56611.5
33	67811.0
34	76600.0
35	85247.5
36	88109.5
37	89461.5
38	92145.5
39	106602.5
40	150932.5
41	209323.0
42	217321.5
43	270718.0
44	338912.0
45	268027.0
46	206821.5
47	205543.0
48	215649.5
49	255219.5
50	299470.5
51	320781.5
52	323336.0
53	332226.0
54	351761.0
55	352669.0
56	351509.0
57	369283.0
58	373640.0
59	372498.0
60	374476.5
61	381627.0
62	389313.0
63	392089.5
64	387656.5
65	370321.0
66	335891.0
67	311975.5
68	292775.5
69	263617.5
70	239698.0
71	235512.0
72	237260.0
73	211599.5
74	173917.5
75	153215.5
76	131849.0
77	96207.5
78	78525.0
79	75594.5
80	63346.0
81	43505.5
82	31234.5
83	26109.0
84	20609.5
85	14823.5
86	10431.0
87	7412.5
88	4375.5
89	2566.0
90	1584.0
91	1139.0
92	984.0
93	728.5
94	509.0
95	186.0
96	19.0
97	19.0
98	15.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	34805.0
25	26705.0
26	24371.0
27	24669.0
28	28626.0
29	35202.0
30	31033.0
31	32350.0
32	40535.0
33	31873.0
34	34083.0
35	27090.0
36	24006.0
37	32691.0
38	31300.0
39	32710.0
40	49029.0
41	43978.0
42	75314.0
43	104802.0
44	297550.0
45	495150.0
46	662503.0
47	685111.0
48	980072.0
49	922813.0
50	890547.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.17905381365882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66974033659318	11.296415355527794
2	9.065793109518342	2.5708873672671575
3	2.9346685346900454	1.2483246923586429
4	1.374636303383995	0.7796416847956279
5	0.7604676507514823	0.5391355871775985
6	0.527997488394133	0.4491902880850266
7	0.3680466390078171	0.365298717029967
8	0.2819386288204162	0.3198098392155088
9	0.2248835735617731	0.2869772662206254
>10	2.2176294377594816	7.303350626899905
>50	1.1387330846394554	11.916501635940762
>100	1.3207295318701908	33.96355918015458
>500	0.06726760059196807	6.647094954561387
>1k	0.04137592036411621	10.877571632657743
>5k	0.0030460800268061017	2.992779981626439
>10k+	0.0030460800268061017	8.443461190481287
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	47223	0.8286309787226278	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	38239	0.6709870189393846	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22036	0.38666989067047464	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	21141	0.3709651551399757	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	18304	0.32118377558687455	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG	15688	0.2752803251424218	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	14727	0.2584174750364894	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	12568	0.22053309066738633	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12413	0.21781327613417142	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	11911	0.20900458648466252	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	10331	0.18128002543640742	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	10011	0.17566492446460888	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG	9893	0.1735943559812582	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA	8826	0.15487150367841757	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8566	0.15030923413883127	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG	7984	0.14009676924637274	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	7059	0.12386561799976768	No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCT	6817	0.11961919788984504	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	6550	0.11493409801650066	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCCGTGG	5772	0.10128238377881556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.024530972370544725	0.0	0.0	0.0	0.0
2	0.044675147106871864	0.0	0.0	0.0	0.0
3	0.056238745670669416	0.0	0.0	0.0	0.0
4	0.06764441951963512	0.0	0.0	0.0	0.0
5	0.0760670709773329	0.0	0.0	0.0	0.0
6	0.0914384098876313	0.0	0.0	0.0	3.5094381073740664E-5
7	0.10235276240156464	0.0	0.0	0.0	3.5094381073740664E-5
8	0.11510956992186938	0.0	0.0	0.0	3.5094381073740664E-5
9	0.12662052691405631	0.0	0.0	0.0	3.5094381073740664E-5
10	0.134920348037996	0.0	0.0	0.0	3.5094381073740664E-5
11	0.1453258320263601	0.0	0.0	0.0	3.5094381073740664E-5
12	0.15569622163365046	0.0	0.0	0.0	3.5094381073740664E-5
13	0.16439962813993814	0.0	0.0	0.0	3.5094381073740664E-5
14	0.173892658220385	0.0	0.0	0.0	3.5094381073740664E-5
15	0.1847719163532446	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
16	0.19403683295671215	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
17	0.2073551505741967	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
18	0.23432518242936642	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
19	0.24818746295349398	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
20	0.2590491738958167	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
21	0.2728061712767231	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
22	0.28403637322032005	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
23	0.2946699706856635	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
24	0.30640904115482975	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
25	0.31700754423909944	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
26	0.3315717123847018	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
27	0.3454164457182925	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
28	0.3572783465212168	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
29	0.3706668529008489	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
30	0.38430101994799715	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
31	0.396636694895417	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
32	0.4095163327494798	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
33	0.42107993131327737	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
34	0.433169945593181	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
35	0.4437158071058401	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
36	0.4527526102323283	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
37	0.45959601454170773	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
38	0.4640354537475359	1.7547190536870332E-5	0.0	0.0	5.2641571610610996E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	12870	0.0	223.19812	44
CTATCAT	4920	0.0	198.52065	44
TGTCGTA	25	9.773627E-5	153.61282	44
AACGCAC	380	0.0	144.85419	44
CTATCTA	570	0.0	139.23969	44
ACTTCTA	1360	0.0	126.128174	44
CCGTACC	20	4.304273E-6	125.894035	43
AGGTACA	195	0.0	124.728355	44
CGACGGG	5835	0.0	117.37055	44
TTAAGCC	12385	0.0	115.474884	43
TTCATAG	210	0.0	109.723434	44
TCTAGAC	120	0.0	106.67557	44
GCTATAC	30	2.5588997E-7	104.911705	43
TTATGAG	460	0.0	102.96512	44
TTTGTAC	320	0.0	92.007675	44
GCATACC	290	0.0	88.28323	44
TATTATG	80	0.0	86.552155	43
CGAATTT	60	2.7284841E-11	83.92936	43
CGATCAT	30	3.2256165E-5	83.92936	43
ACATTGG	940	0.0	83.07076	44
>>END_MODULE