##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840918.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8557567 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.391473300764105 39.0 38.0 39.0 35.0 39.0 2 37.84851991226011 39.0 38.0 39.0 35.0 39.0 3 37.96192480876866 39.0 38.0 39.0 35.0 39.0 4 38.26696793609679 39.0 38.0 40.0 35.0 41.0 5 39.19179119485714 40.0 39.0 41.0 35.0 41.0 6 39.197103569273835 40.0 38.0 41.0 35.0 41.0 7 39.26917639090643 40.0 39.0 41.0 35.0 41.0 8 39.14682911626634 40.0 38.0 41.0 35.0 41.0 9 39.07207539245676 40.0 38.0 41.0 35.0 41.0 10 38.976689402490216 40.0 38.0 41.0 35.0 41.0 11 38.734190804465804 40.0 38.0 41.0 35.0 41.0 12 38.8246073913298 40.0 38.0 41.0 35.0 41.0 13 38.71628302764092 40.0 38.0 41.0 35.0 41.0 14 38.70773468673982 40.0 38.0 41.0 35.0 41.0 15 38.69679816704912 40.0 38.0 41.0 35.0 41.0 16 38.64695561250061 40.0 38.0 41.0 35.0 41.0 17 38.649616415506884 40.0 38.0 41.0 35.0 41.0 18 38.6937497538728 40.0 38.0 41.0 35.0 41.0 19 38.72939539941668 40.0 38.0 41.0 35.0 41.0 20 38.72271721623681 40.0 38.0 41.0 35.0 41.0 21 38.69870875682305 40.0 38.0 41.0 35.0 41.0 22 38.55552728947375 40.0 37.0 41.0 35.0 41.0 23 38.48258085504911 40.0 37.0 41.0 35.0 41.0 24 38.30586579105954 40.0 37.0 41.0 34.0 41.0 25 38.28109300255862 40.0 36.0 41.0 34.0 41.0 26 38.24848463781112 40.0 36.0 41.0 34.0 41.0 27 38.168470524753424 40.0 36.0 41.0 34.0 41.0 28 38.12076259136066 40.0 36.0 41.0 34.0 41.0 29 38.02555981633042 40.0 35.0 41.0 34.0 41.0 30 37.98326343250987 40.0 35.0 41.0 34.0 41.0 31 37.83807958658134 40.0 35.0 41.0 33.0 41.0 32 37.78345718084009 39.0 35.0 41.0 33.0 41.0 33 37.68106679278377 39.0 35.0 41.0 33.0 41.0 34 37.57403317947021 39.0 35.0 41.0 33.0 41.0 35 37.44475089160111 39.0 35.0 41.0 33.0 41.0 36 37.39442043953144 39.0 35.0 41.0 33.0 41.0 37 37.32996682256857 38.0 35.0 41.0 33.0 41.0 38 37.21608840274263 38.0 35.0 41.0 33.0 41.0 39 37.17144773731265 38.0 35.0 41.0 33.0 41.0 40 37.111324086688505 38.0 35.0 41.0 33.0 41.0 41 37.06005671633249 37.0 35.0 41.0 33.0 41.0 42 36.942235684712074 37.0 35.0 40.0 33.0 41.0 43 36.870271440758586 37.0 35.0 40.0 33.0 41.0 44 36.70007056948115 37.0 35.0 40.0 33.0 41.0 45 36.55771240698275 37.0 35.0 40.0 33.0 41.0 46 36.28576934576921 36.0 35.0 40.0 32.0 41.0 47 36.13446066936894 36.0 35.0 40.0 31.0 41.0 48 35.86281562764754 36.0 34.0 40.0 31.0 41.0 49 36.27517343749661 36.0 35.0 40.0 32.0 41.0 50 35.85322033599676 36.0 34.0 39.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 5.0 20 25.0 21 75.0 22 210.0 23 464.0 24 868.0 25 1783.0 26 3281.0 27 6528.0 28 13333.0 29 26508.0 30 50263.0 31 91168.0 32 154668.0 33 273201.0 34 539279.0 35 530925.0 36 806004.0 37 1100657.0 38 1289928.0 39 1735516.0 40 1932877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 48.77671422262893 19.02991819988088 32.19336757749019 2 26.974734758138613 23.442843041719684 29.551553613310887 20.030868586830813 3 27.623014812504536 20.29192409478068 24.76400126344322 27.321059829271565 4 23.56046993263389 23.45045034412234 27.379849903599933 25.60922981964383 5 23.761496696432527 23.64854403126496 28.2334920661445 24.356467206158012 6 21.869101346212073 28.09485453049915 24.609938782833954 25.42610534045483 7 30.134534733996237 23.228740131394822 22.063490709450477 24.573234425158457 8 24.530991109973197 24.549734755217226 24.771748792618276 26.1475253421913 9 25.197699299345246 25.13157069059465 27.730159752181898 21.940570257878203 10 31.552052119486767 21.789078601429587 22.6277632415849 24.03110603749874 11 25.478035988500004 25.675206516057656 25.46962238215605 23.377135113286286 12 26.396860229081465 24.260984459718514 25.152113912751133 24.19004139844888 13 25.04326288067625 23.6477610984524 25.08240952130436 26.22656649956699 14 20.3409684084273 23.219952586991138 24.202778663608477 32.23630034097308 15 22.32830896912639 26.09531424060133 27.054535477198133 24.521841313074148 16 24.211542836883424 28.52705681416225 21.095178103776462 26.166222245177867 17 24.37710391282943 31.223407307240482 19.040890944821115 25.358597835108977 18 23.535567994968666 25.62536758403411 20.46150500486879 30.37755941612844 19 26.5918338705382 25.23727830585492 22.823274419002505 25.34761340460437 20 26.377602419005306 22.87467921665118 23.974711503865525 26.77300686047798 21 24.5240615703038 27.081938125637812 22.96891160770345 25.425088696354937 22 25.918383110526626 26.310585707362854 24.52534698238413 23.245684199726394 23 29.483555314261633 24.032192794984837 22.992177566357352 23.492074324396174 24 26.16874632708105 24.97288072649621 25.38075366514805 23.477619281274688 25 24.312642968320944 28.831286231536573 22.304334845972836 24.551735954169647 26 25.857920946487432 28.481443094434265 24.432358436333978 21.228277522744328 27 22.96915005455927 29.73498646319722 22.85579217103859 24.440071311204914 28 24.543436669228353 24.68874326961314 24.18025363668938 26.587566424469127 29 25.84608560231278 27.961429915672127 22.48573176961045 23.706752712404647 30 24.759298086358054 27.368310486376267 22.211887066866044 25.66050436039963 31 23.829551034091683 29.48721861662971 23.19861473547644 23.484615613802166 32 24.054268961647228 27.515343410043585 23.883403111063657 24.546984517245534 33 22.891957803728545 26.539841014977878 22.814311446965725 27.753889734327853 34 23.506205976938404 28.71259638011894 25.05944095371721 22.721756689225455 35 22.003042378576055 26.093869890158118 22.88851206686878 29.014575664397046 36 22.469945496944103 26.894717635957697 28.466491383548515 22.168845483549685 37 22.506165576013043 30.078190614345523 23.55405984814314 23.861583961498297 38 23.166992750612508 28.513094977300025 23.95537308284043 24.364539189247036 39 23.956994941401078 28.696558809854704 22.914021554470835 24.432424694273383 40 24.28388271435236 29.072329852316454 23.615990680825142 23.027796752506045 41 26.09823892951676 27.002122595718596 21.20163992045495 25.69799855430969 42 23.108439632129798 31.178749182391496 22.25760992909451 23.455201256384196 43 24.329247364293987 25.989437846653917 23.58708082204877 26.094233967003326 44 24.459660207286266 27.41893115702173 22.595341619841015 25.52606701585099 45 23.508246297058946 27.249260070938163 24.87683183338032 24.365661798622572 46 23.23566395715201 30.262295634128066 22.146072109805072 24.355968298914853 47 27.104721378263584 26.9541599031744 23.957695139593007 21.98342357896901 48 22.13544474666688 27.379434190951436 22.174704387269905 28.310416675111778 49 23.263679259470806 28.381422644845465 21.696547870547548 26.65835022513618 50 21.506875864438435 31.62650973897263 25.654991058292882 21.211623338296054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 151479.0 1 118893.0 2 91788.0 3 88163.0 4 69739.0 5 51436.5 6 37036.0 7 32723.0 8 34408.5 9 34683.5 10 33973.5 11 33959.5 12 34269.0 13 32352.5 14 29024.5 15 28389.5 16 31442.5 17 34509.5 18 35478.0 19 37076.0 20 40923.5 21 49106.0 22 58593.5 23 69975.5 24 82062.0 25 92461.0 26 107205.5 27 126369.0 28 137918.5 29 141484.5 30 148329.5 31 172201.0 32 216472.5 33 249225.5 34 263369.5 35 264180.5 36 261210.0 37 272705.5 38 285034.0 39 300653.5 40 339126.0 41 394729.0 42 408185.0 43 430399.0 44 467972.5 45 418241.0 46 375406.5 47 373036.0 48 379767.5 49 427542.0 50 485123.0 51 517038.0 52 512693.5 53 483212.5 54 466824.5 55 451626.5 56 432403.0 57 421478.0 58 415407.0 59 417611.0 60 414796.0 61 401166.5 62 385399.0 63 375027.5 64 352941.5 65 329545.0 66 316864.0 67 311842.5 68 353641.0 69 396925.0 70 354587.0 71 285941.5 72 247485.0 73 219409.5 74 183209.0 75 144852.5 76 113184.5 77 84589.0 78 69596.5 79 64740.5 80 53975.0 81 37490.5 82 24969.0 83 18387.0 84 14678.5 85 11965.5 86 9518.5 87 7315.5 88 4641.5 89 3107.0 90 2343.0 91 1531.0 92 794.0 93 495.0 94 353.0 95 207.5 96 196.0 97 196.0 98 154.0 99 112.0 100 112.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 59618.0 25 40235.0 26 36597.0 27 34712.0 28 40766.0 29 43877.0 30 45144.0 31 43157.0 32 55963.0 33 43143.0 34 57501.0 35 38588.0 36 34827.0 37 40699.0 38 49250.0 39 43868.0 40 56805.0 41 53783.0 42 82262.0 43 103932.0 44 270723.0 45 512885.0 46 1107093.0 47 728319.0 48 1708883.0 49 954677.0 50 2270260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.845381417521104 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70359360730785 14.455084776242499 2 10.736084117638667 4.046512002549823 3 3.4623110985374463 1.9574571971416397 4 1.54411286762759 1.1639758376857683 5 0.8470481529092424 0.7981472760290705 6 0.5868797076054447 0.663598316161672 7 0.40938873046084645 0.5400560741498575 8 0.3199386964967404 0.48234934125644763 9 0.2630218826948757 0.44610729304854835 >10 2.951175112867463 13.90370936430604 >50 1.3283816964830228 17.8753121319174 >100 0.7945023748931126 24.85880762387237 >500 0.029756641376474186 3.8355825879508947 >1k 0.020404554086725153 7.653981419829325 >5k 0.0019129269456304833 2.7349409810530734 >10k+ 0.0014878320688237092 4.584377776805538 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 27689 0.3235615917468131 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 21820 0.2549790144792322 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC 15368 0.17958375318592304 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15103 0.1764870786287738 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC 13721 0.16033762867413134 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10685 0.12486025525713092 No Hit TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10065 0.11761520534983834 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGG 9788 0.11437830402028987 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 9356 0.10933014021391826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01991220168068798 0.0 0.0 0.0 0.0 2 0.0377794295972208 0.0 0.0 0.0 0.0 3 0.04939488057762212 0.0 0.0 0.0 0.0 4 0.06366295466924185 0.0 0.0 0.0 0.0 5 0.07516155000597717 0.0 0.0 0.0 0.0 6 0.09430250443846949 0.0 0.0 0.0 0.0 7 0.10878091868868804 0.0 0.0 0.0 0.0 8 0.12295550826537496 0.0 0.0 0.0 0.0 9 0.13694312881219628 0.0 0.0 0.0 0.0 10 0.14811452834666675 0.0 0.0 0.0 0.0 11 0.1612374171303596 0.0 0.0 0.0 0.0 12 0.17334366181415817 0.0 0.0 0.0 0.0 13 0.18700408655871464 0.0 0.0 0.0 0.0 14 0.19846762520235015 0.0 0.0 0.0 0.0 15 0.21088938012404693 0.0 0.0 0.0 0.0 16 0.22374350092730796 0.0 0.0 0.0 0.0 17 0.23921518814868759 0.0 0.0 0.0 3.505669309980278E-5 18 0.2659517594194705 0.0 0.0 0.0 3.505669309980278E-5 19 0.2794602718272612 0.0 0.0 0.0 3.505669309980278E-5 20 0.2921274236006566 0.0 0.0 0.0 3.505669309980278E-5 21 0.3050633433544838 0.0 0.0 0.0 3.505669309980278E-5 22 0.31712284578081595 0.0 0.0 0.0 3.505669309980278E-5 23 0.32862144111755126 0.0 0.0 0.0 3.505669309980278E-5 24 0.3399798096818874 0.0 0.0 0.0 3.505669309980278E-5 25 0.35339483757474527 0.0 0.0 0.0 3.505669309980278E-5 26 0.3694157463213551 0.0 0.0 0.0 3.505669309980278E-5 27 0.3840343873439729 0.0 0.0 0.0 3.505669309980278E-5 28 0.39747278636556393 0.0 0.0 0.0 3.505669309980278E-5 29 0.410677474099823 0.0 0.0 0.0 3.505669309980278E-5 30 0.42506240383510874 0.0 0.0 0.0 3.505669309980278E-5 31 0.43907339551066327 0.0 0.0 0.0 3.505669309980278E-5 32 0.4524183100173215 0.0 0.0 0.0 3.505669309980278E-5 33 0.4645946680873197 0.0 0.0 0.0 3.505669309980278E-5 34 0.47784609807904516 0.0 0.0 0.0 3.505669309980278E-5 35 0.48715949287922605 0.0 0.0 0.0 3.505669309980278E-5 36 0.4977232430666333 0.0 0.0 0.0 3.505669309980278E-5 37 0.5049682929739259 0.0 0.0 0.0 3.505669309980278E-5 38 0.5114187245042896 0.0 0.0 0.0 3.505669309980278E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGCCA 10345 0.0 115.07881 44 CTATCAT 10545 0.0 102.16132 44 TTAAGCC 10045 0.0 84.3364 43 AACCGGT 60 5.158654E-6 63.310482 44 ACGGTAG 70 4.4929038E-10 61.161213 43 ATTAAGC 13055 0.0 60.90848 42 CCTATCA 13155 0.0 57.889286 43 CTATCGT 80 4.2544343E-7 56.979435 44 GGCCGAG 7480 0.0 56.8787 43 AGGCCGA 9430 0.0 54.81698 42 CCTTAGC 6230 0.0 54.28916 43 CGCGATA 45 0.008013404 50.648384 44 AACCTTA 6270 0.0 49.96685 41 CCCTATC 15950 0.0 49.436398 42 GATACGA 65 1.1395714E-6 49.39944 43 AACGGTA 80 0.0 48.122593 42 CGTATGT 110 0.0 47.19092 41 ACTTCTA 3075 0.0 47.189472 44 GATTAAG 15350 0.0 47.105923 41 CCCCTAT 15030 0.0 46.869556 41 >>END_MODULE