FastQCFastQC Report
Tue 24 May 2016
ERR840917.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840917.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8419608
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA642500.7630996597466295No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225570.26791033501797235No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT221900.2635514622533496No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA188130.2234427065963166No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG170560.2025747516986539No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA154650.18367838502695136No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT133720.1588197455273452No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133190.15819026253953866No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG132440.15729948472660485No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125420.1489618043975444No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC124730.1481422888096453No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA108120.12841452951253787No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG103240.12261853520971523No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG100610.119494874345694No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94710.11248742221728136No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGC85290.10129925288683272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA202900.0193.318644
CTATCAT82700.0160.6323144
TTAAGCC202100.0110.7322643
TTACCGT909.094947E-1294.6933444
ACTTCTA25600.091.96535544
CGACGGG77100.091.3311744
CGGATAC357.746468E-491.3114344
ACGATAG208.007695E-490.5543143
AACGACA5550.085.9314243
GCGATAA1053.6379788E-1181.1657244
ATACGCG351.5988917E-977.87553442
ATTAAGC285200.074.9128742
AATCGCA11700.069.1989844
ACGACAC4800.066.5812544
CGTAATG1158.276402E-1064.84434544
ACATTGG13400.064.39500444
GCATACA3900.061.91747343
GTGTTAG15500.061.14850243
ACGTTAT1251.3145836E-859.65680344
ACCTTAG26750.059.5347542
Produced by FastQC (version 0.11.5)