##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840917.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8419608
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.25033315090204	39.0	37.0	39.0	34.0	39.0
2	37.70980988663605	39.0	38.0	39.0	35.0	39.0
3	37.99266034713255	39.0	38.0	39.0	35.0	40.0
4	38.46580185205772	39.0	38.0	41.0	35.0	41.0
5	39.144840234842285	40.0	38.0	41.0	35.0	41.0
6	39.153486836916876	40.0	38.0	41.0	35.0	41.0
7	39.0854734567215	40.0	38.0	41.0	35.0	41.0
8	38.960814208927545	40.0	38.0	41.0	35.0	41.0
9	38.84270099035489	40.0	38.0	41.0	35.0	41.0
10	38.78463522292249	40.0	38.0	41.0	35.0	41.0
11	38.658844093454235	40.0	38.0	41.0	35.0	41.0
12	38.649821820683336	40.0	38.0	41.0	35.0	41.0
13	38.57091351521353	40.0	38.0	41.0	35.0	41.0
14	38.56727201551426	40.0	38.0	41.0	35.0	41.0
15	38.53231670643099	40.0	37.0	41.0	35.0	41.0
16	38.48050538694913	40.0	37.0	41.0	35.0	41.0
17	38.46058094391093	40.0	37.0	41.0	35.0	41.0
18	38.4731556385998	40.0	37.0	41.0	35.0	41.0
19	38.44581802383199	40.0	37.0	41.0	35.0	41.0
20	38.45475953274784	40.0	37.0	41.0	35.0	41.0
21	38.36428738725128	40.0	36.0	41.0	35.0	41.0
22	38.25927774784764	40.0	36.0	41.0	34.0	41.0
23	38.157192472618675	40.0	35.0	41.0	34.0	41.0
24	37.970770373157514	40.0	35.0	41.0	34.0	41.0
25	37.90541897046161	40.0	35.0	41.0	33.0	41.0
26	37.83354422481436	40.0	35.0	41.0	33.0	41.0
27	37.720964897631404	39.0	35.0	41.0	33.0	41.0
28	37.64306360270568	39.0	35.0	41.0	33.0	41.0
29	37.500267396614646	39.0	35.0	41.0	33.0	41.0
30	37.384264351928046	39.0	35.0	41.0	33.0	41.0
31	37.22340267641696	39.0	35.0	41.0	33.0	41.0
32	37.13361578167765	38.0	35.0	41.0	33.0	41.0
33	37.08691780310571	38.0	35.0	41.0	33.0	41.0
34	36.9001711580426	37.0	35.0	40.0	33.0	41.0
35	36.79929694937668	37.0	35.0	40.0	33.0	41.0
36	36.722916573082934	37.0	35.0	40.0	33.0	41.0
37	36.662148411777714	37.0	35.0	40.0	33.0	41.0
38	36.52135418063832	36.0	35.0	40.0	33.0	41.0
39	36.45720607259668	36.0	35.0	40.0	33.0	41.0
40	36.34151755617152	35.0	35.0	40.0	33.0	41.0
41	36.28533779343907	35.0	35.0	40.0	33.0	41.0
42	36.187642708133794	35.0	35.0	40.0	33.0	41.0
43	36.07779758566097	35.0	35.0	40.0	32.0	41.0
44	35.92105825527389	35.0	35.0	40.0	32.0	41.0
45	35.77883695646099	35.0	34.0	39.0	31.0	41.0
46	35.54283992101028	35.0	34.0	39.0	31.0	41.0
47	35.45930426383702	35.0	34.0	39.0	31.0	41.0
48	35.39535710233222	35.0	34.0	39.0	31.0	41.0
49	35.74721717906137	35.0	34.0	39.0	31.0	41.0
50	35.784319512386666	36.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	28.0
21	80.0
22	239.0
23	535.0
24	1144.0
25	2432.0
26	4570.0
27	9583.0
28	18760.0
29	35700.0
30	64551.0
31	112752.0
32	187139.0
33	337707.0
34	713838.0
35	600679.0
36	868292.0
37	1158530.0
38	1339941.0
39	1420039.0
40	1543063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.97960475119507	20.29954363671088	39.72085161209405
2	24.864399862796464	22.391446252604634	29.952047648774148	22.792106235824754
3	26.113234725417144	16.621842727119837	25.22311014954616	32.041812397916864
4	20.039614670896793	20.76533729361272	29.385121017510553	29.809927017979938
5	21.701592283156177	20.1410683252712	33.60256201951445	24.554777372058176
6	26.67150299633902	23.594649537128095	23.77437286866562	25.959474597867267
7	32.79930609596076	18.84278935551394	21.031442318929813	27.32646222959549
8	26.25022447600886	19.551361536071514	25.574551689342307	28.62386229857732
9	23.482043344535754	24.341798335504457	28.371308973054326	23.804849346905463
10	31.433850602070784	17.563810571703577	21.174560620874512	29.82777820535113
11	28.051982942673813	23.475499096870067	22.37530535863427	26.09721260182184
12	25.199641123434724	19.798760227316997	25.272423609270174	29.729175039978106
13	25.59766440432856	19.14008348132122	23.897953443913302	31.364298670436913
14	22.673704048929594	21.4279215849479	20.446474467694934	35.45189989842758
15	25.579456905832192	20.863845442685694	26.0831620664525	27.473535585029612
16	28.74629080118694	24.017757121234148	17.854263523907527	29.38168855367138
17	25.346168135143582	23.659034957446952	19.55373694357267	31.4410599638368
18	26.70073238564076	22.166625809657646	17.863990817624764	33.26865098707683
19	25.831808321717592	25.654353504343668	21.835897823271583	26.677940350667157
20	32.3913654887496	17.938329195373466	21.360317487464975	28.309987828411963
21	28.633542084144537	23.402621594734576	21.587002625300368	26.37683369582052
22	27.04712618449695	25.290049132928754	21.98117774604233	25.68164693653196
23	33.62519965300047	20.91383589354754	19.83852454888636	25.62243990456563
24	29.665454733759578	22.20067727618673	21.22189061533506	26.91197737471863
25	28.568340543116545	25.314648393646273	20.77030900403128	25.346702059205906
26	29.04274164770645	22.219850735673962	25.181223543728766	23.556184072890822
27	26.74843533617136	28.841579030821812	19.047389373248215	25.362596259758615
28	26.573400418605125	20.290898122474196	24.86092491107183	28.27477654784885
29	33.86229518081883	23.3134956937535	19.375294886980406	23.44891423844727
30	28.615603079166362	25.742743257527135	18.456634022948226	27.185019640358277
31	25.515858974547996	22.84267327170596	25.38797234139797	26.253495412348084
32	24.16338582068938	21.365157384823306	25.321440511274346	29.150016283212963
33	28.87371839538125	20.752401453314754	21.62027174995023	28.753608401353777
34	25.551007249311535	22.969369268345673	27.49021435554917	23.989409126793618
35	24.02857172352651	21.399534539294365	22.170367279869897	32.401526457309224
36	24.49895323302001	21.368066552742317	29.811290911380055	24.32168930285762
37	25.12351301284088	23.47358980708123	21.858083938918817	29.54481324115907
38	25.98077500367255	25.801963376701348	20.897877585953616	27.319384033672485
39	24.84428638072585	27.86898304446705	19.554153932768617	27.73257664203848
40	25.491317740286796	28.48247356203083	20.84676998685648	25.17943871082589
41	28.962479243158533	22.074316759535566	19.62658997829257	29.336614019013325
42	26.62651002618392	26.549401188760154	20.640143834962668	26.183944950093263
43	26.386654165665224	19.847051160689784	26.15270596625767	27.613588707387315
44	25.7982252961311	20.611370408477136	24.6044337610824	28.985970534309363
45	26.850537869698094	24.033888750126643	25.074261685727727	24.041311694447536
46	26.21041110417265	28.641202843288006	20.900523404324733	24.247862648214618
47	31.997491225821673	20.462392903341957	21.436294041509875	26.103821829326495
48	24.483804724955696	20.249905672474537	19.873587278099684	35.39270232447008
49	26.17920334311372	21.922225855210133	18.75457111143617	33.14399969023998
50	22.038359523634604	33.119288947887085	22.29574478326185	22.54660674521646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117150.0
1	90450.5
2	68174.0
3	61355.0
4	39739.0
5	24668.0
6	16008.0
7	13820.0
8	16693.5
9	18222.0
10	17939.5
11	16417.5
12	15059.0
13	13232.5
14	12105.0
15	12601.0
16	13381.5
17	15058.0
18	17449.0
19	19324.5
20	20362.0
21	21216.5
22	22136.0
23	26194.5
24	32485.0
25	38151.0
26	45223.5
27	53393.5
28	58093.0
29	61157.0
30	67698.0
31	82281.5
32	101411.0
33	110971.0
34	122864.5
35	137162.5
36	144768.5
37	155445.5
38	166980.0
39	184070.5
40	242933.5
41	327800.0
42	345599.5
43	443316.0
44	555471.0
45	438398.5
46	333065.5
47	331816.5
48	343119.5
49	400037.5
50	461722.0
51	479012.5
52	478164.5
53	477462.0
54	482447.0
55	473648.0
56	463899.0
57	486804.0
58	495492.5
59	475919.5
60	470248.5
61	497605.5
62	518243.5
63	520725.0
64	522707.5
65	501477.5
66	438801.5
67	394924.0
68	388121.5
69	379595.0
70	362451.0
71	372828.5
72	400686.0
73	368739.5
74	291762.5
75	245128.5
76	204068.5
77	143240.5
78	113081.0
79	109864.0
80	98647.0
81	71129.5
82	45673.5
83	34086.0
84	28274.5
85	23334.0
86	17885.0
87	12538.0
88	7169.0
89	3755.0
90	2686.5
91	2201.0
92	1743.0
93	1485.0
94	1173.5
95	574.0
96	241.5
97	172.5
98	342.0
99	512.0
100	512.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	55510.0
25	38331.0
26	32452.0
27	31591.0
28	36115.0
29	41186.0
30	45183.0
31	48085.0
32	54355.0
33	46524.0
34	47178.0
35	40193.0
36	31872.0
37	42683.0
38	44991.0
39	47988.0
40	63804.0
41	55060.0
42	98176.0
43	148200.0
44	391768.0
45	664124.0
46	1031978.0
47	946305.0
48	1546700.0
49	1210300.0
50	1578956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.904930525109428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3236930993603	13.071516597895691
2	10.581467021155857	3.577579296927527
3	3.306952488968156	1.677114062275331
4	1.5343870880203458	1.0375482848643562
5	0.8629804369555476	0.7294312165631053
6	0.5455013281965898	0.5532997232710959
7	0.41689426875523605	0.4933298054736493
8	0.32671806291206906	0.44185169238614863
9	0.2651791240949898	0.4034551202581656
>10	2.647793055179117	10.773057901572187
>50	1.2625565799293348	15.447059116840084
>100	0.8450612495053408	24.411788156600064
>500	0.047538939392443136	5.42527549315067
>1k	0.027617860027990774	9.382487541319072
>5k	0.0024901349205565452	2.7601291895455393
>10k+	0.003169262626162876	9.815076801057343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	64250	0.7630996597466295	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22557	0.26791033501797235	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT	22190	0.2635514622533496	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	18813	0.2234427065963166	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTG	17056	0.2025747516986539	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA	15465	0.18367838502695136	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT	13372	0.1588197455273452	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13319	0.15819026253953866	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	13244	0.15729948472660485	No Hit
TTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12542	0.1489618043975444	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	12473	0.1481422888096453	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA	10812	0.12841452951253787	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	10324	0.12261853520971523	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG	10061	0.119494874345694	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9471	0.11248742221728136	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGC	8529	0.10129925288683272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.013753609431698007	0.0	0.0	0.0	0.0
2	0.025595015824964772	0.0	0.0	0.0	0.0
3	0.03382580281647317	0.0	0.0	0.0	0.0
4	0.04346995727116987	0.0	0.0	0.0	0.0
5	0.052377735400507955	0.0	0.0	0.0	0.0
6	0.06668956559497782	0.0	0.0	0.0	0.0
7	0.07721262082510255	0.0	0.0	0.0	0.0
8	0.0875693975301463	0.0	0.0	0.0	0.0
9	0.09805682164775367	0.0	0.0	0.0	0.0
10	0.10606194492665216	0.0	0.0	0.0	0.0
11	0.11574173049386623	0.0	0.0	0.0	0.0
12	0.12461387751068696	0.0	0.0	0.0	0.0
13	0.13366418009009445	0.0	0.0	0.0	0.0
14	0.14303516268215813	0.0	0.0	0.0	0.0
15	0.15334443123717872	0.0	0.0	0.0	0.0
16	0.16320237236697954	0.0	0.0	0.0	0.0
17	0.1755901224855124	0.0	0.0	0.0	0.0
18	0.1979427070713981	0.0	0.0	0.0	0.0
19	0.21062738312757553	0.0	0.0	0.0	0.0
20	0.22033092276980115	0.0	0.0	0.0	0.0
21	0.23110339578754735	0.0	0.0	0.0	0.0
22	0.24083068950478453	0.0	0.0	0.0	0.0
23	0.2513181136223919	0.0	0.0	0.0	0.0
24	0.26197181626508026	0.0	0.0	0.0	0.0
25	0.27296995299543636	0.0	0.0	0.0	0.0
26	0.2857377683141543	0.0	0.0	0.0	0.0
27	0.2993369762582771	0.0	0.0	0.0	0.0
28	0.311665341189281	0.0	0.0	0.0	0.0
29	0.3246231891080915	0.0	0.0	0.0	0.0
30	0.3377116844394656	0.0	0.0	0.0	0.0
31	0.3502775901205852	0.0	0.0	0.0	0.0
32	0.3620714883638288	0.0	0.0	0.0	0.0
33	0.373889140682084	0.0	0.0	0.0	0.0
34	0.38575430115036236	0.0	0.0	0.0	0.0
35	0.3955884882051516	0.0	0.0	0.0	0.0
36	0.40556519971001026	0.0	0.0	0.0	0.0
37	0.4118837836630874	0.0	0.0	0.0	0.0
38	0.4168246312654936	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	20290	0.0	193.3186	44
CTATCAT	8270	0.0	160.63231	44
TTAAGCC	20210	0.0	110.73226	43
TTACCGT	90	9.094947E-12	94.69334	44
ACTTCTA	2560	0.0	91.965355	44
CGACGGG	7710	0.0	91.33117	44
CGGATAC	35	7.746468E-4	91.31143	44
ACGATAG	20	8.007695E-4	90.55431	43
AACGACA	555	0.0	85.93142	43
GCGATAA	105	3.6379788E-11	81.16572	44
ATACGCG	35	1.5988917E-9	77.875534	42
ATTAAGC	28520	0.0	74.91287	42
AATCGCA	1170	0.0	69.19898	44
ACGACAC	480	0.0	66.58125	44
CGTAATG	115	8.276402E-10	64.844345	44
ACATTGG	1340	0.0	64.395004	44
GCATACA	390	0.0	61.917473	43
GTGTTAG	1550	0.0	61.148502	43
ACGTTAT	125	1.3145836E-8	59.656803	44
ACCTTAG	2675	0.0	59.53475	42
>>END_MODULE