FastQCFastQC Report
Tue 24 May 2016
ERR840916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3952704
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC255110.6454062839008435No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC168510.42631575751687956No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA147120.3722009034827804No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA145130.36716637522060847No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC84040.21261394731302927No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69340.17542421592914623No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG66270.1676573808714237No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA65700.1662153300626609No Hit
AATTCGTGGAGAAAGAAATGGCTCC62910.1591568708408219No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT58640.1483541393435987No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG54570.13805739058629232No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG48800.12345978853969333No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA48170.12186594290895549No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC48060.12158765240200126No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC42930.10860919512313595No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT39610.10020988164051747No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG39560.10008338595553828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA202.5670852E-7221.7448944
CTATCAT57300.0182.4654444
TAAGCCA52700.0175.8811244
ATTAGTC2350.0165.1291544
GCATTAC1950.0164.887244
CCACGAC850.0130.4381644
TATAGAG459.814357E-8123.1915944
CGTTAGC204.862508E-6122.8418143
ACATTGG7900.0112.2758844
AATACGG303.579496E-4110.8724344
TTTTACA2250.0103.48093444
TTAAGCC50800.0101.6830443
CGACGGG25950.099.9774544
ACTTCTA18100.098.6213344
TTAACGG251.4738982E-598.27344543
ATTCGGT553.9327126E-580.6344944
GATCGTT251.3487679E-679.35261542
TTATCCC5300.077.4015144
CAACACG1301.8189894E-1276.7578444
TAATATG2350.075.4876144
Produced by FastQC (version 0.11.5)