##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840916.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3952704 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.88245312575897 39.0 37.0 39.0 32.0 39.0 2 37.52851465730801 39.0 37.0 39.0 35.0 39.0 3 37.63933676794417 39.0 37.0 39.0 35.0 39.0 4 38.06358609195123 39.0 37.0 40.0 35.0 41.0 5 38.65883051197358 40.0 38.0 41.0 35.0 41.0 6 38.660159981622705 40.0 38.0 41.0 35.0 41.0 7 38.529738882547235 40.0 38.0 41.0 35.0 41.0 8 38.45493338231246 40.0 38.0 41.0 34.0 41.0 9 38.292838016709574 40.0 38.0 41.0 34.0 41.0 10 38.19548466062726 40.0 37.0 41.0 34.0 41.0 11 37.99710805565 40.0 37.0 41.0 33.0 41.0 12 37.99865105001538 39.0 37.0 41.0 33.0 41.0 13 37.91361432578812 39.0 37.0 40.0 33.0 41.0 14 37.89590568886514 39.0 37.0 40.0 33.0 41.0 15 37.864685035864056 39.0 36.0 40.0 33.0 41.0 16 37.81001866064345 39.0 36.0 40.0 33.0 41.0 17 37.83607171192176 39.0 36.0 40.0 33.0 41.0 18 37.91064142419974 39.0 36.0 41.0 33.0 41.0 19 37.98032511414971 39.0 36.0 41.0 33.0 41.0 20 37.974612316024675 40.0 36.0 41.0 33.0 41.0 21 38.00749031549036 40.0 36.0 41.0 33.0 41.0 22 37.89622319303444 40.0 36.0 41.0 33.0 41.0 23 37.763522894707016 39.0 35.0 41.0 33.0 41.0 24 37.55122366865822 39.0 35.0 41.0 33.0 41.0 25 37.45876094388053 39.0 35.0 41.0 33.0 41.0 26 37.3997441874678 39.0 35.0 41.0 33.0 41.0 27 37.290847283196065 39.0 35.0 41.0 33.0 41.0 28 37.231615260435234 39.0 35.0 41.0 33.0 41.0 29 37.06291713625823 38.0 35.0 40.0 32.0 41.0 30 36.9315588314584 38.0 35.0 40.0 32.0 41.0 31 36.793907155522234 38.0 35.0 40.0 32.0 41.0 32 36.73974432746364 38.0 35.0 40.0 32.0 41.0 33 36.66493901161985 38.0 35.0 40.0 32.0 41.0 34 36.53017801914724 37.0 35.0 40.0 32.0 41.0 35 36.39127956892334 37.0 35.0 40.0 31.0 41.0 36 36.32560187316684 37.0 35.0 40.0 32.0 41.0 37 36.23879968795589 36.0 35.0 40.0 32.0 41.0 38 36.15957417611739 36.0 35.0 40.0 32.0 41.0 39 36.139969954390175 36.0 35.0 40.0 32.0 41.0 40 36.042090035225314 35.0 34.0 40.0 31.0 41.0 41 35.97707297200044 35.0 34.0 40.0 31.0 41.0 42 35.88234285461582 35.0 34.0 40.0 31.0 41.0 43 35.79795358467677 35.0 34.0 40.0 31.0 41.0 44 35.57797208128252 35.0 34.0 39.0 31.0 41.0 45 35.41214173557947 35.0 34.0 39.0 30.0 41.0 46 35.19231793121808 35.0 34.0 39.0 30.0 41.0 47 35.121816204120954 35.0 34.0 39.0 30.0 41.0 48 35.012212080783264 35.0 33.0 39.0 29.0 41.0 49 35.29739767040295 35.0 34.0 39.0 30.0 41.0 50 35.38624740735936 36.0 34.0 38.0 30.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 14.0 21 29.0 22 94.0 23 264.0 24 602.0 25 1497.0 26 3297.0 27 7273.0 28 14597.0 29 26989.0 30 47370.0 31 79595.0 32 127748.0 33 207865.0 34 320578.0 35 334079.0 36 466312.0 37 571263.0 38 608155.0 39 687560.0 40 447522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 42.79715860332573 19.854231432457375 37.3486099642169 2 27.646163234079758 19.166803282006445 29.503423479218277 23.68361000469552 3 27.1485798076456 16.722856049934425 26.24975206845744 29.878812073962536 4 21.944597925875552 19.780003764511587 29.97606195657454 28.299336353038324 5 21.784935072294815 20.658895783747024 30.446878896067098 27.109290247891067 6 25.681457554119913 24.223847776104662 24.871328589239162 25.22336608053626 7 30.330275173653277 18.50887392529266 21.626309483330903 29.534541417723158 8 27.370301444277136 21.611635983873317 24.016369553601788 27.001693018247764 9 26.548104791049365 22.40855879924224 27.424466896585226 23.61886951312317 10 30.52292810187659 17.77679279804407 22.08991616877965 29.61036293129969 11 27.62835264163469 20.138872022797557 24.392972506921843 27.839802828645908 12 26.968399353961235 20.890155195025987 24.38649592785091 27.754949523161866 13 28.39658623565033 19.154457303152476 24.388519858810575 28.060436602386616 14 23.164674106636873 21.92886186266414 21.254235075533103 33.65222895516588 15 26.354389299072228 21.75133781836434 24.241810163371706 27.65246271919172 16 26.485337632162693 23.19326213144217 20.487671224559186 29.833729011835945 17 25.842562458509416 25.679762511941195 20.04035718333576 28.437317846213627 18 26.818426069849906 23.402561891808745 19.36054407312058 30.41846796522077 19 29.061498154174963 22.784149787082463 21.962636210553583 26.191715848188984 20 29.966979566393032 18.436619590032542 23.68725308042292 27.909147763151505 21 29.072604475316137 22.931643755768203 20.380934165573745 27.614817603341912 22 29.12363283463674 22.36216018199187 21.569715313871214 26.94449166950017 23 32.778978643480514 21.351940342611034 19.618291680834183 26.25078933307427 24 29.674825132365086 22.10676539401888 22.95107349298101 25.267335980635032 25 28.774264980755294 23.446640884659775 21.623302541463964 26.15579159312097 26 29.759584495942487 22.82401422812767 23.81891492551341 23.597486350416432 27 27.053615575262164 26.26387459523291 20.32599169872632 26.356518130778607 28 27.747533730717798 20.4335424038491 23.170477312813393 28.64844655261971 29 31.534581292202894 23.16376544107075 21.85257099585072 23.449082270875635 30 27.84558880330692 25.280608370613777 19.994413845255632 26.87938898082367 31 26.961339262221056 23.452691204645795 24.108068655567852 25.477900877565297 32 26.128536040539935 22.363584996646257 24.883431185190176 26.624447777623633 33 27.05511562391591 20.108390372889946 23.600763200544744 29.2357308026494 34 26.95533471985869 22.403300402680063 25.066462086121234 25.574902791340016 35 24.0181632080129 21.463349416021167 22.293769412976904 32.22471796298903 36 25.647393158799858 20.617817488934286 29.259494903376833 24.475294448889017 37 24.142787726265034 24.402796937736625 23.178200104896185 28.27621523110216 38 25.6499536069199 23.11104482428153 22.731223138933345 28.507778429865223 39 26.318430439222386 24.49467922557901 22.63704786115501 26.54984247404359 40 25.287785749198022 25.338320308769248 22.965218514918792 26.40867542711394 41 27.338007900541395 22.286627202651747 20.85000041051333 29.525364486293526 42 25.890860225024287 24.260653845769774 20.867543235219856 28.980942693986083 43 26.126035094850675 20.03038270253042 24.777434578598786 29.066147624020118 44 26.908867103820118 21.727309492987942 23.317715664233653 28.046107738958288 45 26.612164624446866 21.653583833742232 27.777724218380946 23.95652732342996 46 24.994413692935183 28.565477725807497 21.52342041995236 24.91668816130496 47 28.077736685624306 20.97216438242637 25.381193606199044 25.56890532575028 48 23.44790100449659 22.93647600290831 20.123966233747897 33.491656758847206 49 25.04967684564969 23.55709739798407 21.229696010639547 30.16352974572669 50 22.597392342844735 29.021783116357568 26.126203612706373 22.254620928091324 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 32282.0 1 24832.5 2 18490.5 3 17052.5 4 11890.0 5 6923.0 6 3642.5 7 4047.0 8 6374.0 9 7302.0 10 6961.0 11 6465.5 12 5959.0 13 5333.0 14 5096.0 15 5250.0 16 5502.0 17 6091.5 18 7189.0 19 8515.0 20 9747.5 21 10889.5 22 11064.5 23 13026.0 24 17355.0 25 20734.0 26 25040.5 27 31717.5 28 35318.0 29 35290.0 30 37855.0 31 46762.0 32 59429.5 33 65885.0 34 70054.0 35 76661.0 36 80899.5 37 82323.0 38 82715.5 39 92397.5 40 117703.0 41 137492.0 42 139938.5 43 171501.0 44 202255.0 45 181818.0 46 164005.0 47 156425.0 48 159079.5 49 198934.5 50 243243.5 51 263181.5 52 261972.5 53 243113.5 54 243366.0 55 247920.0 56 239770.5 57 243137.5 58 248010.0 59 249753.5 60 253554.5 61 255797.0 62 247629.5 63 240581.5 64 238776.5 65 232269.5 66 216094.0 67 205544.0 68 206532.0 69 197801.0 70 174137.5 71 157395.5 72 151793.0 73 138655.5 74 119440.5 75 106054.0 76 91644.5 77 67327.0 78 49445.0 79 42106.5 80 35920.0 81 30409.0 82 22660.0 83 17581.5 84 16349.5 85 14021.0 86 11401.5 87 7837.0 88 4119.0 89 2388.5 90 1320.0 91 465.5 92 170.5 93 92.0 94 63.0 95 33.5 96 31.0 97 31.0 98 15.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 31106.0 25 27340.0 26 19190.0 27 20866.0 28 21971.0 29 26393.0 30 24353.0 31 25978.0 32 31191.0 33 26343.0 34 25494.0 35 21336.0 36 20887.0 37 23037.0 38 24000.0 39 23279.0 40 27777.0 41 27208.0 42 41142.0 43 66379.0 44 181897.0 45 315589.0 46 486673.0 47 429993.0 48 708040.0 49 566023.0 50 709219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.92455174819822 #Duplication Level Percentage of deduplicated Percentage of total 1 74.73142360068746 9.65870151543607 2 9.847036552277597 2.545370669726224 3 3.481189189198052 1.3497842946317529 4 1.7005493626615382 0.8791535295233821 5 1.0339164496618025 0.6681453328483673 6 0.7200144697806077 0.5583518564478581 7 0.5425230722180662 0.49083072650317133 8 0.4118402349135181 0.42582803425039006 9 0.3265582089287193 0.3798556623088342 >10 3.6621367727969827 11.199506084789972 >50 1.7771258462470998 16.876408026391985 >100 1.6671065781848773 36.34697055275805 >500 0.060897334137514934 5.313814652407093 >1k 0.03398138534745308 8.187666506440909 >5k 0.0023551455191304117 2.0066825267154673 >10k+ 0.0013457974395030927 3.1129300288205117 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 25511 0.6454062839008435 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC 16851 0.42631575751687956 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 14712 0.3722009034827804 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 14513 0.36716637522060847 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC 8404 0.21261394731302927 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6934 0.17542421592914623 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG 6627 0.1676573808714237 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 6570 0.1662153300626609 No Hit AATTCGTGGAGAAAGAAATGGCTCC 6291 0.1591568708408219 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT 5864 0.1483541393435987 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG 5457 0.13805739058629232 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG 4880 0.12345978853969333 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA 4817 0.12186594290895549 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC 4806 0.12158765240200126 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 4293 0.10860919512313595 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT 3961 0.10020988164051747 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGG 3956 0.10008338595553828 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.010828030634219006 0.0 0.0 0.0 0.0 2 0.020467001829633586 0.0 0.0 0.0 0.0 3 0.0282338368873561 0.0 0.0 0.0 0.0 4 0.03686084260293713 0.0 0.0 0.0 0.0 5 0.04483007075662635 0.0 0.0 0.0 0.0 6 0.060136048639108826 0.0 0.0 0.0 0.0 7 0.06949672932756917 0.0 0.0 0.0 0.0 8 0.07989467463285893 0.0 0.0 0.0 0.0 9 0.08892646654037338 0.0 0.0 0.0 0.0 10 0.09636441281714998 0.0 0.0 0.0 0.0 11 0.1047890254367643 0.0 0.0 0.0 0.0 12 0.11506047505707485 0.0 0.0 0.0 0.0 13 0.12290320752578487 0.0 0.0 0.0 0.0 14 0.1317326063373326 0.0 0.0 0.0 0.0 15 0.1418269619986723 0.0 0.0 0.0 0.0 16 0.15194661679700783 0.0 0.0 0.0 0.0 17 0.1662153300626609 0.0 0.0 0.0 0.0 18 0.19773805475947603 0.0 0.0 0.0 0.0 19 0.20894557244863263 0.0 0.0 0.0 0.0 20 0.21823035572610547 0.0 0.0 0.0 0.0 21 0.22817291656547012 0.0 0.0 0.0 0.0 22 0.23897564806269328 0.0 0.0 0.0 0.0 23 0.24962658473794141 0.0 0.0 0.0 0.0 24 0.25982213694726447 0.0 0.0 0.0 0.0 25 0.2710549537734169 0.0 0.0 0.0 0.0 26 0.28304674470944446 0.0 0.0 0.0 0.0 27 0.2970624666051392 0.0 0.0 0.0 0.0 28 0.30890246271919175 0.0 0.0 0.0 0.0 29 0.3210713476141902 0.0 0.0 0.0 0.0 30 0.33303783941322196 0.0 0.0 0.0 0.0 31 0.34654757856899987 0.0 0.0 0.0 0.0 32 0.35823577986107735 0.0 0.0 0.0 0.0 33 0.3698733828791632 0.0 0.0 0.0 0.0 34 0.3798918411295154 0.0 0.0 0.0 0.0 35 0.38841765029711306 0.0 0.0 0.0 0.0 36 0.39676636550573985 0.0 0.0 0.0 0.0 37 0.40445730315247486 0.0 0.0 0.0 0.0 38 0.4095677288256343 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTAGA 20 2.5670852E-7 221.74489 44 CTATCAT 5730 0.0 182.46544 44 TAAGCCA 5270 0.0 175.88112 44 ATTAGTC 235 0.0 165.12915 44 GCATTAC 195 0.0 164.8872 44 CCACGAC 85 0.0 130.43816 44 TATAGAG 45 9.814357E-8 123.19159 44 CGTTAGC 20 4.862508E-6 122.84181 43 ACATTGG 790 0.0 112.27588 44 AATACGG 30 3.579496E-4 110.87243 44 TTTTACA 225 0.0 103.480934 44 TTAAGCC 5080 0.0 101.68304 43 CGACGGG 2595 0.0 99.97745 44 ACTTCTA 1810 0.0 98.62133 44 TTAACGG 25 1.4738982E-5 98.273445 43 ATTCGGT 55 3.9327126E-5 80.63449 44 GATCGTT 25 1.3487679E-6 79.352615 42 TTATCCC 530 0.0 77.40151 44 CAACACG 130 1.8189894E-12 76.75784 44 TAATATG 235 0.0 75.48761 44 >>END_MODULE