FastQCFastQC Report
Tue 24 May 2016
ERR840915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682904
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC113150.3072303812426281No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA66910.1816772850989328No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA49290.13383460443172018No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA47240.12826834476271984No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC36920.10024697901438648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGTA252.7343413E-7218.9448444
ACGGTTC500.0218.9448444
CTATCAT27600.0191.8742244
GATCGTT150.0035662225182.4540344
ATAAGTT452.7941496E-8152.0450344
ATGGTGT6600.0130.620544
TCCGGTA550.0126.9677543
GGTAACA1400.0117.2918844
ACTTCTA11000.0115.69244444
CGTATCC601.5552178E-7114.0337644
TAGCACA700.0108.829543
TCGTAGG1150.0107.0925844
CGACGGG5400.0106.4315244
ACGTATC1053.6379788E-12104.25944544
CCAATCG1450.0103.8100644
CGAACTT704.6344212E-897.7432344
TCCGATA206.5557787E-495.22581543
GCTATGC1450.094.3727844
ATCACAC4800.094.0778644
ACGACAC3050.089.7314844
Produced by FastQC (version 0.11.5)