##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR840915.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3682904
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.00547910018833	35.0	33.0	39.0	24.0	39.0
2	35.01489612544883	35.0	35.0	39.0	31.0	39.0
3	35.31475297754164	35.0	35.0	39.0	33.0	39.0
4	35.47506315668288	35.0	35.0	39.0	33.0	39.0
5	36.07841746621688	35.0	35.0	40.0	33.0	41.0
6	36.08060785727784	35.0	35.0	40.0	33.0	41.0
7	36.03481546084286	35.0	35.0	40.0	33.0	41.0
8	35.96070139216227	35.0	35.0	39.0	33.0	41.0
9	35.87348652041975	35.0	35.0	39.0	33.0	41.0
10	35.74991148289502	35.0	35.0	39.0	33.0	41.0
11	35.53460149925168	35.0	35.0	39.0	32.0	41.0
12	35.52327266743852	35.0	35.0	39.0	32.0	41.0
13	35.30365385576165	35.0	34.0	38.0	32.0	41.0
14	35.3052449914524	35.0	34.0	38.0	32.0	41.0
15	35.27663794657694	35.0	34.0	37.0	32.0	40.0
16	35.19982600686849	35.0	34.0	37.0	32.0	40.0
17	35.19059524766325	35.0	35.0	37.0	32.0	40.0
18	35.15592939701931	35.0	35.0	36.0	33.0	40.0
19	35.12519061045305	35.0	35.0	36.0	33.0	39.0
20	35.061666825961254	35.0	35.0	36.0	33.0	39.0
21	34.9524815200179	35.0	35.0	36.0	33.0	39.0
22	34.817338165751806	35.0	35.0	35.0	33.0	39.0
23	34.758736583956576	35.0	35.0	35.0	32.0	39.0
24	34.63713661827732	35.0	34.0	35.0	32.0	38.0
25	34.54851925793111	35.0	34.0	35.0	32.0	37.0
26	34.51898698696278	35.0	34.0	35.0	32.0	37.0
27	34.45023629476565	35.0	34.0	35.0	32.0	37.0
28	34.37838801237346	35.0	34.0	35.0	32.0	37.0
29	34.30157329554541	35.0	34.0	35.0	32.0	37.0
30	34.23292298750298	35.0	34.0	35.0	32.0	36.0
31	34.07181086854082	35.0	34.0	35.0	31.0	36.0
32	34.069592456893794	35.0	34.0	35.0	31.0	36.0
33	34.05304069396855	35.0	34.0	35.0	31.0	36.0
34	34.0609651073383	35.0	34.0	35.0	31.0	36.0
35	33.99895599748534	35.0	34.0	35.0	31.0	35.0
36	34.03359070160372	35.0	34.0	35.0	31.0	35.0
37	34.010130785091846	35.0	34.0	35.0	31.0	35.0
38	34.02604489852643	35.0	34.0	35.0	31.0	35.0
39	34.00507154963357	35.0	34.0	35.0	31.0	35.0
40	33.99362835706646	35.0	34.0	35.0	31.0	35.0
41	33.97059260439815	35.0	34.0	35.0	31.0	35.0
42	33.944944987424876	35.0	34.0	35.0	31.0	35.0
43	33.907320682195035	35.0	34.0	35.0	31.0	35.0
44	33.79244263756022	35.0	34.0	35.0	31.0	35.0
45	33.684019073682464	35.0	34.0	35.0	31.0	35.0
46	33.495269645637286	35.0	33.0	35.0	30.0	35.0
47	33.4540310049282	35.0	33.0	35.0	30.0	35.0
48	33.3941887381792	35.0	33.0	35.0	30.0	35.0
49	33.47760669719771	35.0	33.0	35.0	30.0	35.0
50	33.236152829842545	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	18.0
21	100.0
22	305.0
23	840.0
24	2111.0
25	4473.0
26	9363.0
27	18014.0
28	32860.0
29	56501.0
30	93708.0
31	154471.0
32	254826.0
33	491189.0
34	1331591.0
35	343056.0
36	360996.0
37	344524.0
38	93653.0
39	60192.0
40	30109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.21921179590888	16.387041313050787	42.39374689104033
2	29.493274872220397	16.67385302467835	29.908843673362107	23.924028429739142
3	25.46327571937797	16.666521853406984	26.250942191270806	31.619260235944246
4	20.95892263279195	19.57479749675799	32.32587653655919	27.140403333890866
5	23.4815515147829	20.432245858159757	29.328785110879892	26.75741751617745
6	26.537808207870743	22.231315288153045	26.077763634349417	25.153112869626792
7	30.432425064568612	18.723594207179985	22.00092644282881	28.843054285422593
8	27.128103257646686	19.260860451426375	24.11005011262851	29.500986178298426
9	28.37413084891705	20.3177166714093	24.032746984444884	27.275405495228767
10	31.404701290068925	17.427443126402427	23.050858778833224	28.116996804695425
11	31.3502062502851	18.86546594752402	22.609739488186495	27.17458831400438
12	30.41154480268831	18.989199827092968	22.771323933504647	27.82793143671407
13	28.01666836822247	19.911515478003228	26.0121089227414	26.059707231032903
14	26.502455670851045	22.153632025162754	21.277394143317338	30.066518160668863
15	27.661595306312627	23.529312737991543	22.364145250595726	26.444946705100104
16	26.49555893935872	23.968612812063526	20.876080397425508	28.659747851152247
17	28.693498391486717	24.08952283306869	18.83236706685811	28.384611708586483
18	27.59021142011847	20.40468608467666	20.06131574431481	31.94378675089006
19	28.885765146199848	19.518591850344187	23.04933823960657	28.546304763849395
20	29.42615392635811	19.567520630459008	23.698608489387723	27.307716953795158
21	30.163751213716132	23.99237123747999	18.8697017353697	26.974175813434183
22	30.56148083143085	21.5892675996985	21.606563733401686	26.242687835468963
23	32.12220573764616	21.36849073448561	20.70480251453744	25.804501013330782
24	31.387106479017646	22.56911936884589	20.949473567597742	25.094300584538722
25	28.500545992708798	22.439944903129355	21.268316182545842	27.79119292161601
26	31.497985966343183	22.820685876152734	21.319517687167178	24.361810470336902
27	28.69451625095525	23.891456144077733	20.14431499347534	27.269712611491677
28	29.804197665007266	21.41163948421718	19.722733471555443	29.061429379220115
29	31.142352766139318	23.379910697618435	20.962070727530776	24.515665808711468
30	30.965607905857457	24.47790833202785	18.854712241798666	25.70177152031603
31	27.879198304735624	23.715981139901636	21.71907934277467	26.68574121258807
32	27.336571478091383	21.48364613773812	24.008575069505515	27.171207314664986
33	26.357844858146386	21.08919662957705	22.895418499528386	29.657540012748186
34	28.165192016063394	21.21074207833155	23.801691363387018	26.822374542218036
35	25.47412574585084	22.246599372959956	22.950205404664896	29.32906947652431
36	25.918457003901946	22.56831324795101	25.15198224520658	26.36124750294046
37	26.31810195898997	23.36952727891336	23.856942990136123	26.455427771960544
38	27.724779580709857	21.323333064826183	22.3989254992979	28.552961855166064
39	26.343117670281003	24.507701121107512	21.41140223034203	27.73777897826945
40	27.362882297829362	23.54464525443415	21.713970157152655	27.378502290583835
41	27.91468324968651	22.600699445795957	20.208149776423927	29.2764675280936
42	25.847619850177615	26.047197112555935	21.530094365731394	26.575088671535052
43	26.33556259259623	22.647350644126334	21.829253784794105	29.187832978483335
44	27.0956414461594	24.1062851648264	21.063064353724176	27.735009035290027
45	27.084065182236593	20.448613011568604	26.346328316125557	26.120993490069246
46	26.54243851990186	23.77881487240822	22.246448653585386	27.432297954104534
47	26.716096744473656	22.049098984241663	24.373047088434856	26.86175718284983
48	24.721983698899272	22.94320180740469	20.391382036553814	31.943432457142222
49	26.014407963900016	24.35414282774588	20.361688626456274	29.26976058189783
50	23.727576117511244	26.533422031319777	24.927593165313652	24.811408685855326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34173.0
1	23398.0
2	14392.0
3	14210.5
4	11031.5
5	7467.0
6	4000.5
7	3521.5
8	4948.0
9	5912.0
10	6132.5
11	6412.0
12	6324.0
13	5237.5
14	4014.0
15	3666.0
16	4085.0
17	4713.0
18	5731.0
19	6695.5
20	7432.0
21	8877.0
22	11470.5
23	16261.5
24	20697.5
25	22233.0
26	23305.5
27	25311.0
28	25931.5
29	23972.0
30	24274.0
31	32333.5
32	45599.0
33	54438.0
34	61125.5
35	66983.5
36	68178.0
37	67724.0
38	68491.5
39	79426.0
40	95564.0
41	107770.0
42	119137.5
43	126060.0
44	126338.0
45	125416.5
46	132330.0
47	142073.5
48	151720.0
49	175472.5
50	204974.0
51	227569.0
52	240105.0
53	232462.5
54	224380.0
55	226243.5
56	232480.5
57	248131.0
58	257547.5
59	250794.5
60	251222.0
61	264211.0
62	268692.5
63	265324.5
64	249991.0
65	233578.0
66	223532.0
67	209429.0
68	194064.0
69	179221.5
70	166285.0
71	158246.5
72	147657.5
73	130593.0
74	116142.5
75	106435.0
76	94942.0
77	76940.0
78	59552.5
79	43963.0
80	31920.0
81	24290.0
82	18476.0
83	15246.5
84	12319.5
85	9595.5
86	7259.0
87	5170.0
88	3206.0
89	1880.5
90	1274.0
91	705.5
92	449.0
93	391.0
94	291.0
95	147.5
96	104.0
97	105.0
98	53.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	25346.0
25	18592.0
26	14256.0
27	16990.0
28	18566.0
29	20863.0
30	24301.0
31	24485.0
32	27243.0
33	25000.0
34	21868.0
35	17362.0
36	18622.0
37	20301.0
38	20119.0
39	20717.0
40	22783.0
41	26517.0
42	37501.0
43	64563.0
44	182284.0
45	326560.0
46	436939.0
47	451861.0
48	637824.0
49	621782.0
50	539659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.036093194834459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.2257314556171	9.415413663205111
2	10.541154424873115	2.7483094292757517
3	3.9096116044407965	1.5289818369328947
4	1.949289047659578	1.0164445475584145
5	1.2334832801570939	0.8039901497198989
6	0.8740016700026964	0.6836140333557661
7	0.65160180212446	0.594603927289158
8	0.5163140343897243	0.5384574296084328
9	0.43808192145590974	0.513978907956425
>10	4.017108596842671	11.923885186710411
>50	1.795069963981154	17.147551082354177
>100	1.741946372641872	38.243646044003796
>500	0.07107055054256564	6.259979002735531
>1k	0.03481739092236802	7.738607352275031
>5k	3.5894217445740225E-4	0.3130855154040756
>10k+	3.5894217445740225E-4	0.5294518916151719
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	11315	0.3072303812426281	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	6691	0.1816772850989328	No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAA	4929	0.13383460443172018	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	4724	0.12826834476271984	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC	3692	0.10024697901438648	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.019984229836020707	0.0	0.0	0.0	0.0
2	0.03537968950588992	0.0	0.0	0.0	0.0
3	0.04512743204818806	0.0	0.0	0.0	0.0
4	0.05927387735330598	0.0	0.0	0.0	0.0
5	0.0703249392327359	0.0	0.0	0.0	0.0
6	0.09324163757730312	0.0	0.0	0.0	0.0
7	0.10483574918053797	0.0	0.0	0.0	0.0
8	0.11596826851853863	0.0	0.0	0.0	0.0
9	0.12460275912703671	0.0	0.0	0.0	0.0
10	0.13304718233220306	0.0	0.0	0.0	0.0
11	0.14192604531641334	0.0	0.0	0.0	0.0
12	0.1519181602344237	0.0	0.0	0.0	0.0
13	0.16220895250052675	0.0	0.0	0.0	0.0
14	0.17437326631375674	0.0	0.0	0.0	0.0
15	0.18485412598319154	0.0	0.0	0.0	0.0
16	0.19490054587358238	0.0	0.0	0.0	0.0
17	0.20915560112346127	0.0	0.0	0.0	0.0
18	0.23272395913659438	0.0	0.0	0.0	0.0
19	0.242634616596034	0.0	0.0	0.0	0.0
20	0.25507045527116645	0.0	0.0	0.0	0.0
21	0.2698685602448503	0.0	0.0	0.0	0.0
22	0.2816527392514168	0.0	0.0	0.0	0.0
23	0.2951203724017786	0.0	0.0	0.0	0.0
24	0.30818071825928667	0.0	0.0	0.0	0.0
25	0.32257153594011684	0.0	0.0	0.0	0.0
26	0.3371795735104689	0.0	0.0	0.0	0.0
27	0.35176045859463073	0.0	0.0	0.0	0.0
28	0.36555392157927546	0.0	0.0	0.0	0.0
29	0.38008050169105684	0.0	0.0	0.0	0.0
30	0.395584571305687	0.0	0.0	0.0	0.0
31	0.4107628110860343	0.0	0.0	0.0	0.0
32	0.4235244795954497	0.0	0.0	0.0	0.0
33	0.43859410943103594	0.0	0.0	0.0	0.0
34	0.4501339160618903	0.0	0.0	0.0	0.0
35	0.46018033595228114	0.0	0.0	0.0	0.0
36	0.47038967075981347	0.0	0.0	0.0	0.0
37	0.4772592497659456	0.0	0.0	0.0	0.0
38	0.48225530722495075	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCAGTA	25	2.7343413E-7	218.94484	44
ACGGTTC	50	0.0	218.94484	44
CTATCAT	2760	0.0	191.87422	44
GATCGTT	15	0.0035662225	182.45403	44
ATAAGTT	45	2.7941496E-8	152.04503	44
ATGGTGT	660	0.0	130.6205	44
TCCGGTA	55	0.0	126.96775	43
GGTAACA	140	0.0	117.29188	44
ACTTCTA	1100	0.0	115.692444	44
CGTATCC	60	1.5552178E-7	114.03376	44
TAGCACA	70	0.0	108.8295	43
TCGTAGG	115	0.0	107.09258	44
CGACGGG	540	0.0	106.43152	44
ACGTATC	105	3.6379788E-12	104.259445	44
CCAATCG	145	0.0	103.81006	44
CGAACTT	70	4.6344212E-8	97.74323	44
TCCGATA	20	6.5557787E-4	95.225815	43
GCTATGC	145	0.0	94.37278	44
ATCACAC	480	0.0	94.07786	44
ACGACAC	305	0.0	89.73148	44
>>END_MODULE