FastQCFastQC Report
Tue 24 May 2016
ERR840914.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840914.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661253
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA161790.9739034331314977No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT158090.9516310881003677No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC91470.5506084864858032No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC75920.45700444182794553No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC64980.39115053516833376No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA62130.37399480994165246No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA50730.3053719090349273No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC49850.30007470264914493No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC49460.2977270770918096No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT46870.28213643557001855No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44650.2687730285513405No Hit
AATTCGTGGAGAAAGAAATGGCTCC43450.261549565298001No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43030.25902135315933217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA42680.25691450971044144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA41930.2523998451771043No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA41880.2520988675415484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA39820.23969858895664897No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG39370.23698979023664668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35910.21616213785618446No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32100.19322764202683154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA31130.1873886758970488No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC31120.18732848036993763No Hit
AATTCGTGGAGAAAGAAATGGCTC29200.17577093916459444No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT28560.17191842542948005No Hit
AAAAAAAAAAAAAAAAAAAAAAAA28190.16969119092636703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27160.16349105163391728No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA26880.16180557687480474No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25610.1541607449316871No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG25580.1539801583503536No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT24910.14994705803390573No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC24450.14717806378679227No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG24090.14501102481079042No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC23800.1432653545245667No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23520.14157987976545414No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT22660.1364030644338942No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG21940.13206898648189047No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC21820.13134664015655653No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA21370.1286378414365542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20900.12580865166232957No Hit
AGCAGTGACGTGACACGCAGCCCACGGTCTGTACTGACGCGCCCTCGCT20880.12568826060810726No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA20430.12297946188810495No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG20220.12171535581877053No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC20190.12153476923743704No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA19970.12021046764099147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19830.1193677302614352No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA19730.11876577499032356No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT19000.11437150151120871No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC18180.10943546828809339No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG18030.10853253538142595No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT17490.10528197691742316No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC17190.1034761111040883No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17190.1034761111040883No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17150.10323532899564364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGC202.1735832E-7229.2233444
GGACTAT252.399247E-9229.2233444
TTGTACA100.0018010035229.2233444
TGGTTAT151.9799756E-5229.2233444
TAACCGC450.0229.2233444
CAAATCA100.0018010035229.2233444
CTAATGA100.0018010035229.2233444
ATAACCC750.0213.9417944
CATCGCA357.8216544E-11196.4771444
CTATCAT20850.0186.8967444
TAAGCCA21600.0185.1827444
TGTATCT256.609207E-7183.3786844
GACTCGC550.0166.707944
ACAGGGA600.0152.8155744
GGTCATC1100.0145.8694244
CCTATAT1050.0130.9847744
CGACGGG7350.0127.8660944
ACACGAC551.8208084E-9125.0309244
AACTTCG152.2198336E-4125.0110643
ATGAGAT152.2198336E-4125.0110643
Produced by FastQC (version 0.11.5)