##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR840914.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1661253 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.39006520981452 35.0 33.0 39.0 25.0 39.0 2 35.25485582268324 35.0 35.0 39.0 31.0 39.0 3 35.6018264526836 35.0 35.0 39.0 33.0 39.0 4 35.816085207972534 35.0 35.0 39.0 33.0 39.0 5 36.58194748181042 35.0 35.0 40.0 34.0 41.0 6 36.58653076924466 35.0 35.0 40.0 34.0 41.0 7 36.557385298927976 35.0 35.0 40.0 33.0 41.0 8 36.51460915345224 35.0 35.0 40.0 33.0 41.0 9 36.427914351396204 35.0 35.0 40.0 33.0 41.0 10 36.285731312449094 35.0 35.0 40.0 33.0 41.0 11 36.237606794389535 35.0 35.0 40.0 33.0 41.0 12 36.29398351726076 35.0 35.0 40.0 33.0 41.0 13 36.210557332326864 35.0 35.0 40.0 33.0 41.0 14 36.16384259351225 35.0 35.0 40.0 33.0 41.0 15 36.11502642884618 35.0 35.0 40.0 33.0 41.0 16 36.116137036321376 35.0 35.0 40.0 33.0 41.0 17 36.12149925387644 35.0 35.0 40.0 33.0 41.0 18 36.184994097828564 35.0 35.0 40.0 33.0 41.0 19 36.13829019420883 35.0 35.0 40.0 33.0 41.0 20 36.135927519769716 35.0 35.0 40.0 33.0 41.0 21 36.11237722369802 35.0 35.0 40.0 33.0 41.0 22 36.11025232159099 35.0 35.0 40.0 33.0 41.0 23 36.050615408971424 35.0 35.0 40.0 33.0 41.0 24 35.92414761628722 35.0 35.0 40.0 33.0 41.0 25 35.87115770884605 35.0 35.0 40.0 33.0 41.0 26 35.90147385224639 35.0 35.0 40.0 33.0 41.0 27 35.845281479197546 35.0 35.0 40.0 33.0 41.0 28 35.80945209632764 35.0 35.0 40.0 33.0 41.0 29 35.770872323986474 35.0 35.0 40.0 33.0 41.0 30 35.73287044488746 35.0 35.0 39.0 33.0 41.0 31 35.66424813184302 35.0 35.0 39.0 33.0 41.0 32 35.595942584174615 35.0 35.0 39.0 32.0 41.0 33 35.56490711806288 35.0 35.0 39.0 32.0 41.0 34 35.51135589415349 35.0 35.0 38.0 32.0 41.0 35 35.486294950079206 35.0 35.0 38.0 32.0 41.0 36 35.47007635899772 35.0 35.0 38.0 33.0 41.0 37 35.40652794745118 35.0 35.0 37.0 33.0 41.0 38 35.32412558941429 35.0 35.0 37.0 32.0 41.0 39 35.3116788527342 35.0 35.0 37.0 32.0 41.0 40 35.249415374532674 35.0 35.0 37.0 32.0 41.0 41 35.2051064236271 35.0 34.0 36.0 32.0 41.0 42 35.172663460968955 35.0 35.0 36.0 32.0 40.0 43 35.09416171297704 35.0 34.0 36.0 32.0 40.0 44 34.94664720725908 35.0 34.0 36.0 32.0 40.0 45 34.79779752629309 35.0 34.0 36.0 31.0 40.0 46 34.61318912673813 35.0 34.0 36.0 31.0 40.0 47 34.540244280934054 35.0 34.0 36.0 31.0 39.0 48 34.42612738136706 35.0 34.0 36.0 30.0 39.0 49 34.64194281522154 35.0 34.0 36.0 31.0 39.0 50 34.73608570949977 35.0 34.0 37.0 31.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 4.0 20 20.0 21 75.0 22 186.0 23 461.0 24 1111.0 25 2301.0 26 3998.0 27 7069.0 28 11995.0 29 19688.0 30 32897.0 31 55193.0 32 90517.0 33 180077.0 34 515865.0 35 110509.0 36 83954.0 37 108621.0 38 125073.0 39 153768.0 40 157870.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 41.79730600937967 19.763455656663975 38.439238333956354 2 24.117939892358358 24.194508602843754 30.051232413124314 21.636319091673574 3 23.178468300734444 20.93449944108453 27.744660205278787 28.142372052902235 4 20.42909779545921 23.790536420400745 29.357117790005493 26.423247994134545 5 21.70957704816786 22.080441690699733 29.101166408728833 27.10881485240358 6 23.874388789666597 27.41894220808029 23.012689819070303 25.69397918318281 7 28.627788783526654 22.508191106351653 21.12333280963225 27.740687300489448 8 25.33184289208206 24.87658412054034 24.590053411491205 25.201519575886394 9 25.183400722225933 26.01879424747465 24.279926055814496 24.517878974484923 10 29.31323525074146 20.67511691476253 21.318366317472414 28.693281517023593 11 25.959020089053265 25.347614120185185 23.275142317274973 25.418223473486577 12 24.54412422430539 24.02830875248984 25.02671176515558 26.400855258049194 13 25.762301106453982 23.713847318861127 23.104006433697936 27.419845140986954 14 21.132482529753144 24.916192777379482 22.47700982330807 31.4743148695593 15 26.551464466881324 25.200556447452616 23.78554019165052 24.46243889401554 16 26.083925807808928 26.374910985864286 17.88758244529882 29.65358076102797 17 24.88711833778479 27.7711462372077 20.302807579579994 27.03892784542752 18 28.223154450285413 26.173316165569 17.294475916672535 28.309053467473046 19 26.2838351533451 27.302644449701518 20.165020018022542 26.248500378930846 20 28.85677256865751 23.79529186704253 22.998122501509403 24.349813062790556 21 27.098461221740454 28.945922144309144 18.383894566330355 25.571722067620044 22 26.203112951489025 28.592514204639507 19.025458494281125 26.178914349590336 23 29.45932979504025 25.010338581781344 20.46118201140946 25.06914961176895 24 29.08942828094216 26.50667899471062 20.768600568366168 23.63529215598106 25 26.497647511689472 28.05581755125943 18.9266912709518 26.519843666099298 26 29.910205835853198 25.3038419622711 22.579318178329675 22.206634023546027 27 26.245305377965835 32.471182171766515 15.965367836715691 25.31814461355196 28 25.90255691707643 26.14224059949618 19.80522113658227 28.14998134684512 29 30.54733918457696 27.881337231927144 19.397935069678667 22.17338851381723 30 25.597346618827398 30.277580982242775 18.619483898604745 25.505588500325082 31 26.069367076649492 26.876758129283417 23.232742965727528 23.82113182833956 32 22.52633999484858 25.55578465018855 23.78766652985221 28.130208825110657 33 24.992169864971512 26.059986291589688 22.205809019926477 26.742034823512324 34 24.530388975757212 26.832370530668285 22.597909591394032 26.039330902180467 35 23.001886408003102 26.113736599601683 20.831662733911305 30.05271425848391 36 23.717976271641497 25.92343997053298 25.89407875550771 24.464505002317814 37 23.45392379012624 30.72788062255579 18.880946070098307 26.937249517219662 38 23.720856598734702 28.96888988047724 20.086009898218947 27.224243622569116 39 23.426706049454452 29.86037133879958 19.41787989325758 27.29504271848839 40 22.75232326843212 31.823259630578992 21.75531173028827 23.669105370700617 41 26.014717471624348 28.7597954428376 19.368520167439385 25.856966918098667 42 24.52338088619957 29.222147688908663 18.77681649699242 27.477654927899348 43 24.17828261554812 25.295826626906976 23.18145427867677 27.344436478868133 44 24.381245710889548 26.88790340798553 20.833443196321717 27.897407684803206 45 25.236483795882364 26.248161098997862 26.81962916936522 21.69572593575455 46 25.146692867834087 30.950880324164316 20.408861760180848 23.49356504782075 47 26.930680619494375 24.002163579806453 22.726270229920274 26.340885570778898 48 22.186996840677075 24.951587090865246 19.92602263897245 32.935393429485224 49 24.977405167882438 27.070310579675617 18.29614167243355 29.656142580008392 50 20.28831920908783 33.74948294664009 21.03663643542559 24.925561408846484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 74408.0 1 57226.5 2 27251.5 3 12655.5 4 9786.0 5 7224.0 6 4036.5 7 2430.0 8 2451.0 9 2171.0 10 1985.0 11 2552.5 12 3308.0 13 3497.0 14 3731.0 15 4007.0 16 3641.5 17 3869.0 18 5552.5 19 7283.0 20 8136.0 21 8862.0 22 9846.0 23 11162.5 24 12613.5 25 13977.0 26 15118.0 27 15051.0 28 13864.0 29 13942.5 30 15934.5 31 19411.0 32 24608.5 33 27453.5 34 30020.5 35 34955.5 36 38216.0 37 37659.5 38 35846.0 39 39612.5 40 51128.0 41 64069.0 42 69300.5 43 79964.0 44 90178.5 45 77810.5 46 68605.5 47 66252.5 48 66216.5 49 82091.0 50 98780.0 51 102321.0 52 96171.5 53 90411.5 54 95135.0 55 98751.5 56 94000.5 57 93191.5 58 93926.5 59 88463.0 60 86544.0 61 96731.5 62 101832.0 63 100025.0 64 98603.0 65 92664.0 66 80362.5 67 71511.5 68 68660.5 69 62596.0 70 57519.0 71 60324.5 72 62495.5 73 54105.5 74 44025.0 75 39463.0 76 34928.0 77 26659.0 78 19526.5 79 16466.0 80 14303.0 81 10687.5 82 7386.5 83 5490.5 84 3999.5 85 2918.5 86 2191.0 87 1980.0 88 1614.5 89 1222.5 90 821.0 91 394.0 92 223.5 93 94.5 94 46.5 95 123.0 96 220.5 97 220.0 98 110.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 16824.0 25 15704.0 26 13579.0 27 12200.0 28 12287.0 29 12597.0 30 11411.0 31 9798.0 32 14734.0 33 9787.0 34 10922.0 35 9211.0 36 6856.0 37 8306.0 38 10093.0 39 9930.0 40 11409.0 41 13607.0 42 18050.0 43 30685.0 44 72305.0 45 133047.0 46 195868.0 47 180981.0 48 292180.0 49 236359.0 50 292523.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.437263228823308 #Duplication Level Percentage of deduplicated Percentage of total 1 69.73688799776359 6.581253687938639 2 10.849546998841246 2.0478006188310958 3 4.400574974439282 1.245881531758677 4 2.358987655926494 0.8904954985009277 5 1.4717285551824704 0.6944544888316392 6 1.058044626477557 0.5991027388746444 7 0.7606290581806716 0.5024779649080071 8 0.6119740509179004 0.46202881661772355 9 0.47440335547936985 0.4029362408066265 >10 4.081717405871518 8.408254717839943 >50 1.572589131354509 10.934345231070346 >100 2.4488288318509333 44.95526270259763 >500 0.1115448625356007 7.06444449566515 >1k 0.058029119238173776 11.164169836451334 >5k 0.003223839957676321 2.100671128294369 >10k+ 0.0012895359830705285 1.9464203010132457 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 16179 0.9739034331314977 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 15809 0.9516310881003677 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 9147 0.5506084864858032 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC 7592 0.45700444182794553 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 6498 0.39115053516833376 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 6213 0.37399480994165246 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 5073 0.3053719090349273 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC 4985 0.30007470264914493 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC 4946 0.2977270770918096 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT 4687 0.28213643557001855 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4465 0.2687730285513405 No Hit AATTCGTGGAGAAAGAAATGGCTCC 4345 0.261549565298001 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4303 0.25902135315933217 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 4268 0.25691450971044144 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 4193 0.2523998451771043 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 4188 0.2520988675415484 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 3982 0.23969858895664897 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 3937 0.23698979023664668 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 3591 0.21616213785618446 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3210 0.19322764202683154 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3113 0.1873886758970488 No Hit AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 3112 0.18732848036993763 No Hit AATTCGTGGAGAAAGAAATGGCTC 2920 0.17577093916459444 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT 2856 0.17191842542948005 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 2819 0.16969119092636703 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2716 0.16349105163391728 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA 2688 0.16180557687480474 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2561 0.1541607449316871 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG 2558 0.1539801583503536 No Hit CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT 2491 0.14994705803390573 No Hit CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC 2445 0.14717806378679227 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG 2409 0.14501102481079042 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC 2380 0.1432653545245667 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2352 0.14157987976545414 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 2266 0.1364030644338942 No Hit AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG 2194 0.13206898648189047 No Hit CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC 2182 0.13134664015655653 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 2137 0.1286378414365542 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2090 0.12580865166232957 No Hit AGCAGTGACGTGACACGCAGCCCACGGTCTGTACTGACGCGCCCTCGCT 2088 0.12568826060810726 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 2043 0.12297946188810495 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG 2022 0.12171535581877053 No Hit CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC 2019 0.12153476923743704 No Hit CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA 1997 0.12021046764099147 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1983 0.1193677302614352 No Hit CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA 1973 0.11876577499032356 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT 1900 0.11437150151120871 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 1818 0.10943546828809339 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG 1803 0.10853253538142595 No Hit AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 1749 0.10528197691742316 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC 1719 0.1034761111040883 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1719 0.1034761111040883 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1715 0.10323532899564364 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03750181339025423 0.0 0.0 0.0 0.0 2 0.07482304019917496 0.0 0.0 0.0 0.0 3 0.09751675392008322 0.0 0.0 0.0 0.0 4 0.12153476923743704 0.0 0.0 0.0 0.0 5 0.14820138774768202 0.0 0.0 0.0 0.0 6 0.18245264267393346 0.0 0.0 0.0 0.0 7 0.20273853531039523 0.0 0.0 0.0 0.0 8 0.2238069697993021 0.0 0.0 0.0 0.0 9 0.24102289055309456 0.0 0.0 0.0 0.0 10 0.2602854592286665 0.0 0.0 0.0 0.0 11 0.279728614485572 0.0 0.0 0.0 0.0 12 0.29634257996825286 0.0 0.0 0.0 0.0 13 0.3130167409780449 0.0 0.0 0.0 0.0 14 0.3274034719576127 0.0 0.0 0.0 0.0 15 0.3426329403167368 0.0 0.0 0.0 0.0 16 0.35701967129630463 0.0 0.0 0.0 0.0 17 0.37598126233632084 0.0 0.0 0.0 0.0 18 0.41312190256390807 0.0 0.0 0.0 0.0 19 0.4278698067061429 0.0 0.0 0.0 0.0 20 0.4452663140412689 0.0 0.0 0.0 0.0 21 0.4636861453372846 0.0 0.0 0.0 0.0 22 0.47897580922351984 0.0 0.0 0.0 0.0 23 0.4933625402030877 0.0 0.0 0.0 0.0 24 0.508652204089323 0.0 0.0 0.0 0.0 25 0.5247846053551145 0.0 0.0 0.0 0.0 26 0.5452510845729097 0.0 0.0 0.0 0.0 27 0.5650554129924822 0.0 0.0 0.0 0.0 28 0.5834150487613867 0.0 0.0 0.0 0.0 29 0.6023164442742918 0.0 0.0 0.0 0.0 30 0.6187498231756391 0.0 0.0 0.0 0.0 31 0.6359657439294316 0.0 0.0 0.0 0.0 32 0.6544457707525585 0.0 0.0 0.0 0.0 33 0.6709393451810169 0.0 0.0 0.0 0.0 34 0.6844833387810285 0.0 0.0 0.0 0.0 35 0.6958000978779271 0.0 0.0 0.0 0.0 36 0.7058527509054913 0.0 0.0 0.0 0.0 37 0.7124742588877191 0.0 0.0 0.0 0.0 38 0.716507359204167 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATCGC 20 2.1735832E-7 229.22334 44 GGACTAT 25 2.399247E-9 229.22334 44 TTGTACA 10 0.0018010035 229.22334 44 TGGTTAT 15 1.9799756E-5 229.22334 44 TAACCGC 45 0.0 229.22334 44 CAAATCA 10 0.0018010035 229.22334 44 CTAATGA 10 0.0018010035 229.22334 44 ATAACCC 75 0.0 213.94179 44 CATCGCA 35 7.8216544E-11 196.47714 44 CTATCAT 2085 0.0 186.89674 44 TAAGCCA 2160 0.0 185.18274 44 TGTATCT 25 6.609207E-7 183.37868 44 GACTCGC 55 0.0 166.7079 44 ACAGGGA 60 0.0 152.81557 44 GGTCATC 110 0.0 145.86942 44 CCTATAT 105 0.0 130.98477 44 CGACGGG 735 0.0 127.86609 44 ACACGAC 55 1.8208084E-9 125.03092 44 AACTTCG 15 2.2198336E-4 125.01106 43 ATGAGAT 15 2.2198336E-4 125.01106 43 >>END_MODULE