FastQCFastQC Report
Tue 24 May 2016
ERR840913.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR840913.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654378
Sequences flagged as poor quality0
Sequence length24-50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA109140.41116977310692No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA108480.4086833148858226No Hit
CTTTTCCAAGTCTTTTGCCAACCAGTTTGTTAGACTTTCCCCTATTCT81230.3060227292420296No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC68190.2568963425706512No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC59570.22442169125874312No Hit
CATTTTCCGGCAAGCCAAGGGCTGTCTGTGCCTCAGGAGTGGGGTCAGC51090.19247447047858293No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCG47220.17789478363669378No Hit
AGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACAGATG45670.17205537417805603No Hit
CACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAAAAAAAA41410.15600641656915482No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA37000.1393923548190951No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36160.13622777162860752No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA35300.13298784121929882No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG32680.12311735555373049No Hit
CAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCT29850.11245572409054023No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT28710.1081609326177357No Hit
CAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGATCTCACCGT27110.10213315511204508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT1200.083.4244444
TCACGGG1350.080.3346444
CAATTAG1150.079.7972944
GAACACG750.077.8628144
TGGTCGA1150.076.17013544
CCATAAC1100.075.8403944
TACTTAG600.069.5203644
TCCCACG555.456968E-1268.25635544
TACACAC750.066.7395544
TACCGGA1350.064.8856744
GTAGTTA1500.061.1779244
GAACACA15850.061.0551144
GAGCATC1600.059.96131544
TAAGCCA16350.059.69822344
CACACTT1400.059.58888244
CTCTAAT1200.058.17630843
TACGTTA303.6848905E-758.17630843
CGACTAA1450.058.17630443
TACGCCT1500.055.6162944
CTTATAT900.055.6162944
Produced by FastQC (version 0.11.5)